Lipase and phospholipase protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1999
|
| Schriftenreihe: | Methods in molecular biology
109 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 362 S. graph. Darst. |
| ISBN: | 0896035468 |
Internformat
MARC
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| 490 | 1 | |a Methods in molecular biology |v 109 | |
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Datensatz im Suchindex
| _version_ | 1804126981397151744 |
|---|---|
| adam_text | Contents
Preface v
Contributors xiii
Part I. Lipase Activity, Purification, and Expression
1 Phospholipase A2 and Phosphatidylinositol Specific
Phospholipase C Assays by HPLC and TLC with
Fluorescent Substrate
H. Stewart Hendrickson /
2 Fluorometric Phospholipase Assays Based on Polymerized
Liposome Substrates
Wonhwa Cho, Shih Kwang Wu, Edward Yoon,
and Lenka Lichtenbergova 7
3 Triglyceride Lipase Assays Based on a Novel Fluorogenic
Alkyldiacyl Glycerol Substrate
Albin Hermetter 19
4 Purification and Assay of Mammalian Group I and Group Ha
Secretory Phospholipase A2
Wonhwa Cho, Sang Kyou Han, Byung ln Lee, Yana Snitko,
and Rajiv Dua 31
5 Determination of Plasmalogen Selective Phospholipase A2 Activity
by Radiochemical and Fluorometric Assay Procedures
Akhlaq A. Farooqui, Hsiu Chiung Yang, Yutaka Hirashima,
and Lloyd A. Horrocks 39
6 Human Plasma Platelet Activating Factor Acetylhydrolase
Diana M. Stafforini and Larry W. Tjoelker 49
7 Assays for Pancreatic Triglyceride Lipase and Colipase
Mark E. Lowe 59
8 Bile Salt Activated Lipase
Chi Sun Wang, Azar Dashti, and Deborah Downs 71
9 Determining Lipoprotein Lipase and Hepatic Lipase Activity Using I
Radiolabeled Substrates
Veronique Briquet Laugier, Osnat Ben Zeev,
and Mark H. Doolittle 81
10 Lysosomal Acid Lipase: Assay and Purification
Martin Merkel, Anne Christine Tilkorn, Heiner Greten,
and Detlev Ameis 95
11 Hormone Sensitive Lipase and Neutral Cholesteryl Ester Lipase
Cecilia Holm and Torben Osterlund 109
12 Lecithin Cholesterol Acyltransferase: Assay of Cholesterol
Esterification and Phospholipase A2 Activities
John S. Parks, Abraham K. Gebre, and James W. Furbee 123
13 Purification of Lipases and Phospholipases by Heparin Sepharose
Chromatography
Akhlaq A. Farooqui and Lloyd A. Horrocks 133
14 Large Scale Lipoprotein Lipase Purification from Adipose Tissue
Andre Bensadoun, Jean Hsu, and Barry Hughes 145
15 Purification of Rat Hepatic Lipase Essentially Free
of Apolipoprotein E and Apolipoprotein B
Andre Bensadoun, Barry Hughes, Kristan Mel ford, Jean Hsu,
and Dawn L. Braesaemle 151
16 High Level Expression and Purification of Human Hepatic Lipase
from Mammalian Cells
John S. Hill 157
17 High Level Baculoviral Expression of Hormone Sensitive Lipase
Cecilia Holm and Juan Antonio Contreras 165
18 High Level Baculoviral Expression of Lysosomal Acid Lipase
Anne Christine Tilkorn, Martin Merkel, Heiner Greten,
and Detlev Ameis 177
19 One Step Purification and Biochemical Characterization of
Recombinant Pancreatic Lipases Expressed in Insect Cells
Sofiane Bezzine, Francine Ferrato, Veronique Lopez,
Alain de Caro, Robert Verger, and Frederic Carriere 187
20 Modulation of the Expression Level of Human Acidic Lipases
by Various Signal Peptides
Stephane Canaan, Liliane Dupuis, Mireille Riviere,
Robert Verger, and Catherine Wicker Planquart 203
Part II. Biochemical Characterization
21 Immune detection of Lipoprotein Lipase: Antibody Production,
Immunoprecipitation, and Western Blotting Techniques
Mark H. Doolittle and Osnat Ben Zeev 215
22 Immunological Characterization of Digestive Lipases
Alain De Caro, Sofiane Bezzine, Veronique Lopez,
Mustapha Aoubala, Cecile Daniel, Robert Verger,
and Frederic Carriere 239
23 Determining Lipase Subunit Structure by
Sucrose Gradient Centrifugation
Osnat Ben Zeev and Mark H. Doolittle 257
24 Detecting Ligands Interacting with Lipoprotein Lipase
Sivaram Pillarisetti 267
25 Monolayer Techniques for Studying Lipase Kinetics
Stephane Ransac, Margarita Ivanova, Ivan Panaiotov,
and Robert Verger 279
26 Efficient Immobilization of Phospholipase A2
Wonhwa Cho andZhen Shen 303
Part III. Molecular Genetic Characterization
27 An Introduction to Internet Resources for the Molecular
and Genetic Analysis of the Lipases
Karen Reue 309
28 Techniques for the Measurement of Lipoprotein Lipase
Messenger RNA
Gouri Ranganathan and Philip A. Kern 329
29 In Vitro Transcription and Translation of Lipoprotein Lipase
Gouri Ranganathan and Philip A. Kern 337
30 Induced Lipase Mutations in the Mouse
Clay F. Semenkovich 343
Index 357
i
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| isbn | 0896035468 |
| language | English |
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| physical | XV, 362 S. graph. Darst. |
| publishDate | 1999 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Lipase and phospholipase protocols ed. by Mark Doolittle ... Totowa, NJ Humana Press 1999 XV, 362 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 109 Lipase Laboratory manuals Phospholipases Laboratory manuals Lipasen (DE-588)4167788-2 gnd rswk-swf Phospholipasen (DE-588)4174399-4 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Lipasen (DE-588)4167788-2 s Biochemische Analyse (DE-588)4255721-5 s DE-604 Phospholipasen (DE-588)4174399-4 s Doolittle, Mark Sonstige oth Methods in molecular biology 109 (DE-604)BV035362695 109 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008376760&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lipase and phospholipase protocols Methods in molecular biology Lipase Laboratory manuals Phospholipases Laboratory manuals Lipasen (DE-588)4167788-2 gnd Phospholipasen (DE-588)4174399-4 gnd Biochemische Analyse (DE-588)4255721-5 gnd |
| subject_GND | (DE-588)4167788-2 (DE-588)4174399-4 (DE-588)4255721-5 (DE-588)4143413-4 |
| title | Lipase and phospholipase protocols |
| title_auth | Lipase and phospholipase protocols |
| title_exact_search | Lipase and phospholipase protocols |
| title_full | Lipase and phospholipase protocols ed. by Mark Doolittle ... |
| title_fullStr | Lipase and phospholipase protocols ed. by Mark Doolittle ... |
| title_full_unstemmed | Lipase and phospholipase protocols ed. by Mark Doolittle ... |
| title_short | Lipase and phospholipase protocols |
| title_sort | lipase and phospholipase protocols |
| topic | Lipase Laboratory manuals Phospholipases Laboratory manuals Lipasen (DE-588)4167788-2 gnd Phospholipasen (DE-588)4174399-4 gnd Biochemische Analyse (DE-588)4255721-5 gnd |
| topic_facet | Lipase Laboratory manuals Phospholipases Laboratory manuals Lipasen Phospholipasen Biochemische Analyse Aufsatzsammlung |
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