Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
[1998]
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Jena, Univ., Diss., 1998 |
| Beschreibung: | 89 Bl. graph. Darst. |
Internformat
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| 246 | 1 | 3 | |a Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI 3 K gamma |
| 264 | 1 | |c [1998] | |
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Datensatz im Suchindex
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| adam_text |
CONTENTS
CONTENTS
1. INTRODUCTION
1.1. SIGNAL TRANSDUCTION 1
1.2. THE PHOSPHOINOSITIDE 3-KINASE (PI3K) FAMILY 1
1.2.1. CLASS I PI3KS --2
1.2.2. CLASS II PI3KS 6
1.2.3. CLASS IHPI3KS . 7
1.2.4. PI3K RELATED PROTEINS 7
1.3. REGULATION OF PI3KS 8
1.3.1. REGULATION OF PI3K ACTIVITY VIA TYROSINE KINASE RECEPTORS 9
1.3.2. REGULATION OF PI3K ACTIVITY BY SMALL G-PROTEINS AND G-PROTEIN
COUPLED RECEPTORS
11
1.3.3. DOWNSTREAM TARGETS OF PI3K ACTIVATION OR LIPID-REGULATED
KINASES 14'
1.4. MAPK CASCADE AND CELL SIGNALLING 17
1.5. AIM OF THIS WORK 22
2. MATERIALS AND METHODS 23
2.1. MATERIALS 23
2.1.1. BACTERIAL STRAINS 23
2.1.2. ESTABLISHED CELL LINES 23
2.1.3. ANTIBODIES 23
2.1.4. PLASMIDS AND PHAGMIDS 24
2.1.5. OLIGONUCLEOTIDES 26
2.1.6. OTHER REAGENTS AND MATERIALS 27
2.2. METHODS 28
2.2.1. PURIFICATION OF PLASMID DNA 28
2.2.2. SEQUENCING OF DOUBLE STRANDED DNA 28
2.2.3. CONSTRUCTION OF PCDNA3-PI3KY WILD TYPE EXPRESSION VECTOR 28
2.2.4. CONSTRUCTION OF CATALYTICALLY INACTIVE PI3KY MUTANTS 29
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/955028817
CONTENTS
2
.
2
.
5
.
CONSTRUCTION OF CHIMERIC MUTANTS BETWEEN PI3KY AND MAMMALIAN PLLOCPK,
FRAP(MTOR) AND HUMAN HOMOLOGUE OF VPS34 30
2.2.6. DEAE-DEXTRAN TRANSFECTION OF COS-7 CELLS 31
2.2.7. TRANSFECTION OF HEK 293 CELLS BY CA
3(P04)2 32
2.2.8. TRANSFECTION OF SF9 INSECT CELL WITH RECOMBINANT BACULOVIRUSES 32
2.2.9. VIRUS INFECTION OF SF9 CELLS WITH RECOMBINANT VIRUSES 32
2.2.10. PKB ASSAY 3 3
2.2.11. MAPK ASSAY 34
2.2.12. METABOLIC LABELLING IN COS-7 CELLS 35
2.2.13. FISHING OF GST-FUSION PROTEINS ON GLUTATHION-SEPHAROSE BEADS 36
2.2.14. PI3-KINASE PROTEIN KINASE ASSAY 36
2.2.15. PI3-KINASE LIPID KINASE ASSAY 36
2.2.16. WESTERN BLOTS 37
3. RESULTS 39
3.1. CONSTRUCTION AND CHARACTERISATION OF PI3KY CHIMERIC MUTANTS 3 9
3.2. IN VITRO CHARACTERISATION OF CONSTRUCTED PI3KY MUTANT 40
3.3. ACTIVATION OF MAPK VIA SOLUBLE FORM OF PI3KY CHIMERIC MUTANTS 42
3.4. CONSTRUCTION AND CHARACTERISATION UNDER IN VIVO CONDITIONS OF
MEMBARENE
ATTACHED PI3KY WILD TYPE AND CHIMERIC MUTANTS FORMS 46
3.4. 1.CONSTRUCTION OF MEMBRANE ATTACHED PI3KY WILD TYPE AND CHIMERIC
MUTANTS
FORMS 46
3.4.2. CHARACTERISATION UNDER IN VIVO CONDITIONS OF THE MEMBRANE BOUND
FORMS
OF THE WILD TYPE AND SUBSTRATE MUTANTS OF PI3KY 47
3.5. LIPID PRODUCTION AND DIFFERENTIAL ACTIVATION OF MAPK AND PKB
PATHWAYS 49
3.5.1. LIPIDS AND MAPK CASCADE ACTIVATION 49
3.5.2. ACTIVATION OF PKB VIA WILD TYPE PI3KYCAAX 50
3.6. MAPK CASCADE OF PROTEINS AND PI3KY 52
4. DISCUSSION
61
4.1. DIFFERENTIAL ACTIVATION OF MAPK AND PKB PATHWAYS VIA PI3KY PROTEIN
AND
CONTENTS
LIPID KINASE ACTIVITIES 61
4.2. PROTEIN KINASE ACTIVITY OF PI3KY IS SUFFICIENT FOR MAPK ACTIVATION
62
4.3. MEMBRANE ATTACHED PI3KY WILD TYPE CAN ACTIVATE PKB PATHWAY
BUT NOT MAPK 64
4.4. PI3KY AND MAPK CASCADE OF PROTEINS 65
5. REFERENCES 71
6. APPENDIX
84 |
| any_adam_object | 1 |
| author | Bondeva, Cvetanka T. 1962- |
| author_GND | (DE-588)120623102 |
| author_facet | Bondeva, Cvetanka T. 1962- |
| author_role | aut |
| author_sort | Bondeva, Cvetanka T. 1962- |
| author_variant | c t b ct ctb |
| building | Verbundindex |
| bvnumber | BV012339719 |
| ctrlnum | (OCoLC)64640646 (DE-599)BVBBV012339719 |
| format | Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV012339719 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T16:03:02Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008367313 |
| oclc_num | 64640646 |
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| owner | DE-29T |
| owner_facet | DE-29T |
| physical | 89 Bl. graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
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| spelling | Bondeva, Cvetanka T. 1962- Verfasser (DE-588)120623102 aut Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg von Tzvetanke Todorova Bondeva Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI 3 K gamma [1998] 89 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 1998 Proteinkinase B (DE-588)4522666-0 gnd rswk-swf Regulation (DE-588)4049075-0 gnd rswk-swf Enzymaktivität (DE-588)4152470-6 gnd rswk-swf Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase (DE-588)4451113-9 gnd rswk-swf Mitogene (DE-588)4466670-6 gnd rswk-swf Proteinkinasen (DE-588)4175996-5 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Signaltransduktion (DE-588)4318717-1 s Proteinkinasen (DE-588)4175996-5 s Regulation (DE-588)4049075-0 s Mitogene (DE-588)4466670-6 s Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase (DE-588)4451113-9 s Enzymaktivität (DE-588)4152470-6 s DE-604 Proteinkinase B (DE-588)4522666-0 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008367313&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bondeva, Cvetanka T. 1962- Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg Proteinkinase B (DE-588)4522666-0 gnd Regulation (DE-588)4049075-0 gnd Enzymaktivität (DE-588)4152470-6 gnd Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase (DE-588)4451113-9 gnd Mitogene (DE-588)4466670-6 gnd Proteinkinasen (DE-588)4175996-5 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| subject_GND | (DE-588)4522666-0 (DE-588)4049075-0 (DE-588)4152470-6 (DE-588)4451113-9 (DE-588)4466670-6 (DE-588)4175996-5 (DE-588)4318717-1 (DE-588)4113937-9 |
| title | Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg |
| title_alt | Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI 3 K gamma |
| title_auth | Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg |
| title_exact_search | Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg |
| title_full | Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg von Tzvetanke Todorova Bondeva |
| title_fullStr | Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg von Tzvetanke Todorova Bondeva |
| title_full_unstemmed | Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg von Tzvetanke Todorova Bondeva |
| title_short | Differentielle Aktivierung des MAPK- und PKB-Weges über die Proteinkinase- bzw. Lipidkinaseaktivität von PI3Kg |
| title_sort | differentielle aktivierung des mapk und pkb weges uber die proteinkinase bzw lipidkinaseaktivitat von pi3kg |
| topic | Proteinkinase B (DE-588)4522666-0 gnd Regulation (DE-588)4049075-0 gnd Enzymaktivität (DE-588)4152470-6 gnd Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase (DE-588)4451113-9 gnd Mitogene (DE-588)4466670-6 gnd Proteinkinasen (DE-588)4175996-5 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| topic_facet | Proteinkinase B Regulation Enzymaktivität Phosphatidylinositolkinase Phosphatidylinositol-3-Kinase Mitogene Proteinkinasen Signaltransduktion Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008367313&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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