Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
1998
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | PST: Modulation of the epidermal growth factor receptor (EGF-R) signal transduction by monoclonal anti-EGF-R antibodies, the ganglioside GM3 and the receptor like protein tyrosinphosphatase RPTP [RPTP sigma]. - Jena, Univ., Diss., 1998 |
| Beschreibung: | II, 86 Bl. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Suárez Pestana, Eduardo Raúl |d 1965- |e Verfasser |0 (DE-588)12062284X |4 aut | |
| 245 | 1 | 0 | |a Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP |c von Eduardo Raúl Suárez Pestana |
| 246 | 1 | 3 | |a Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP sigma |
| 264 | 1 | |c 1998 | |
| 300 | |a II, 86 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 338 | |b nc |2 rdacarrier | ||
| 500 | |a PST: Modulation of the epidermal growth factor receptor (EGF-R) signal transduction by monoclonal anti-EGF-R antibodies, the ganglioside GM3 and the receptor like protein tyrosinphosphatase RPTP [RPTP sigma]. - Jena, Univ., Diss., 1998 | ||
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Datensatz im Suchindex
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CONTENTS
1
CONTENTS
CONTENTS PAG.
1 .INTRODUCTION 1
1.1 GENERAL OVERVIEW 1
1.2 PROTEIN TYROSINE KINASE (PTK) 1
1.2.1 NON-RECEPTOR PTKS 1
1.2.2 RECEPTOR PROTEIN TYROSINE KINASES (RPTKS) , 2
1.3 THE EPIDERMAL GROWTH FACTOR RECEPTOR (EGF-R) 4
1.3.1 EGF-R STRUCTURE 4
1.3.2 THE ERBB RECEPTOR FAMILY 5
1.3.3 THE LIGANDS FOR THE ERBB FAMILY 6
1.4 ROLE OF EGF/EGF-R IN MALIGNANCY DEVELOPMENT 6
1.5 THE IMMUNOTHERAPY WITH ANTIBODIES AGAINST EGF-R -8
1.6 INHIBITORS OF EGF-R TYROSINE KINASE 9
1.7 GANGLIOSIDES 9
1.7.1 GANGLIOSIDES AND EGF-R 10
1.8 PROTEIN TYROSINE PHOSPHATASES (PTPASES) 1 1
1.8.1 THE CYTOSOLIC PTPASES 11
1.8.2 RECEPTOR PTPASE FAMILIES 12
1.8.3 TYPE II RECEPTOR-LIKE PTPASES 13
1.9 AIMS OF THIS WORK 15
2. MATERIALS AND METHODS 18
2.1 MATERIALS
18
2.1.1 CHEMICALS 18
2.1.2 ENZYMES 19
2.1.3 MEDIA 19
2.1.3.1 MEDIUM FOR E. COLI XL-1 BLUE 19
2.13.2 CELL CULTURE MEDIA 20
2.1.3.3 TRANSFECTION* MEDIA 20
2.1.4 PRIMERS 20
2.1.5 PLASMIDS 21
2.1.6 CELL LINES 21
2.1.7 ANTIBODIES 23
2.1.8 GENERAL SOLUTIONS 24
2.1.9 FURTHER REAGENTS 27
2.2 METHODS 28
2.2.1 EGF-R ASSAY 28
2.2.2 GROWTH ASSAY 28
2.2.3 SOFT AGAR ASSAY 29
2.2.4 EGF-R AUTOPHOSPHORYLATION 29
2.2.5 DEPHOSPHORYLATION ASSAYS 29
2.2.6 CELL TRANSFECTIONS 30
2.2.7 PTPASES IMMUNOBLOTTING AND IMMUNOPRECIPITATION 31
2.2.8 PTPASE ASSAYS 31
2.2.9 15S-METABOLIC LABELLED OF THE CELLS WITH 15S-METHIONINE 32
2.2.10 DNA PREPARATIONS 32
2.2.11 DNA RESTRICTIONS 33
2.2.12 PURIFICATION OF DNA FRAGMENTS 33
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/955028663
CONTENTS
II
2.2.13 LIGATIONS 33
2.2.14 TRANSFORMATION 33
2.2.15 DNA SEQUENCING 34
2.2.16 PCR 34
2.2.17 CONSTRUCTIONS OF PCDNA3-SIGMA 34
2.2.18 PCDNA3-SIGMA-VSV 35
2.2.19 PNRTIS 33-SIGMA AND PNRTIS 33-A-SIGMA 36
3. RESULTS 38
3.1 EGF-R EXPRESSION IN LUNG CANCER CELL LINES 38
3.2 GROWTH MODULATION OF HI25 CELLS BY EGF AND EGF-R-SPECIFIC TYROSINE
KINASE
INHIBITORS 39
3.3 GROWTH MODULATION OF HI25 CELLS BY AEGF-R ANTIBODY AEGF-R
IOR EGF/R3 41
3.4 GROWTH MODULATION OF HI25 CELLS BY GANGLIOSIDE IN COMBINATION WITH
AEGF-R
ANTIBODY AEGF-R IOR EGF/R3 42
3.5 EFFECT OF THE AEGF-R ANTIBODY AEGF-R
IOR EGF/R3 AND GANGLIOSIDE GM3 ON EGF-
R SIGNALLING ACTIVITY 43
3.6 GM
3 ACTIVATES EGF-R-DIRECTED PTPASE ACTIVITY _ 45
3.7 OVEREXPRESSION OF THE PTP RPTPA LEADS TO DEPHOSPHORYLATION OF THE
EGF-R 46
3.8 GM3 ACTIVATES RPTPA TOWARDS EGF-R IN HEK 293 CELLS 48
3.9 ACTIVITY OF THREE PTPASES TOWARDS THE SUBSTRATE
32P-RAYTIDE IS UNAFFECTED BY GM3-
TREATMENT 50
3.10 GENERATION OF STABLE CELL LINES WITH INDUCIBLE EXPRESSION OF RPTPA
AND RPTPA
ANTISENSE RNA 53
3.11 EGF-R PHOSPHORYLATION IS MODULATED BY RPTPA EXPRESSION IN A431
CELLS 57
3.12 SOFT AGAR COLONY FORMATION EFFICIENCY OF A431 CELLS IS AFFECTED BY
MODULATION
OF THE RPTPA LEVEL 5 8
3.13 EFFECT OF
GM3 ON EGF-R PHOSPHORYLATION IN A431 CELL LINES WITH REGULABLE
LEVEL OF RPTPA 60
4. DISCUSSION 62
4.1 EGF-R A POSSIBLE TARGET FOR ANTIMOUR THERAPY IN NSCLC 62
4.2 EFFECT OF THE AEGF-R
IOR EGF/R3 ON THE GROWTH OF HI25 NSCLC CELL LINE 63
4.3 COMBINATION OF MAB AEGF-R IOR EGF/R3 AND THE GANGLIOSIDE GM3 64
4.4 ACTIVATION OF PTPASE ACTIVITY BY GANGLIOSIDE
GM3 65
4.5 ACTIVATION BY GANGLIOSIDES A NOVEL WAY TO REAGULATE TRANSMEMBRANE
PTPASES 66
4.6 RPTPA REGULATES EGF-R PHOSPHORYLATION IN A431 CELLS AND
EGF-R-RELATED
TRANSFORMATION 68
4.7 CONCLUSIONS 71
5. REFERENCES 72
6. APPENDIX 80
6.1 ABBREVIATIONS 80
6.2 PUBLICATIONS 82
6.3 ACKNOWLEDGEMENTS 83
6.4 LEBENSLAUF 84
6.5 SELBSTANDIGKEITSERKLARUNG 85
6.6 ERKLARUNG ZUR BEWERBUNG 86 |
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| author | Suárez Pestana, Eduardo Raúl 1965- |
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| id | DE-604.BV012339716 |
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| indexdate | 2024-10-09T16:03:03Z |
| institution | BVB |
| language | German |
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| physical | II, 86 Bl. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
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| spelling | Suárez Pestana, Eduardo Raúl 1965- Verfasser (DE-588)12062284X aut Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP von Eduardo Raúl Suárez Pestana Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP sigma 1998 II, 86 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier PST: Modulation of the epidermal growth factor receptor (EGF-R) signal transduction by monoclonal anti-EGF-R antibodies, the ganglioside GM3 and the receptor like protein tyrosinphosphatase RPTP [RPTP sigma]. - Jena, Univ., Diss., 1998 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008367311&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Suárez Pestana, Eduardo Raúl 1965- Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP |
| subject_GND | (DE-588)4113937-9 |
| title | Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP |
| title_alt | Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP sigma |
| title_auth | Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP |
| title_exact_search | Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP |
| title_full | Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP von Eduardo Raúl Suárez Pestana |
| title_fullStr | Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP von Eduardo Raúl Suárez Pestana |
| title_full_unstemmed | Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP von Eduardo Raúl Suárez Pestana |
| title_short | Modulation der Signaltransduktion des Rezeptors für den epidermalen Wachstumsfaktor (EGF-R) durch monoklonale Anti-EGF-R Antikörper, das Gangliosid GM3 und die rezeptorähnliche Protein-Tyrosinphosphatase RPTP |
| title_sort | modulation der signaltransduktion des rezeptors fur den epidermalen wachstumsfaktor egf r durch monoklonale anti egf r antikorper das gangliosid gm3 und die rezeptorahnliche protein tyrosinphosphatase rptp |
| topic_facet | Hochschulschrift |
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