Plant biochemistry and molecular biology:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Chichester [u.a.]
Wiley
1999
|
| Ausgabe: | 2. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 364 S. Ill., graph. Darst. |
| ISBN: | 0471976822 0471976830 |
Internformat
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| 245 | 1 | 0 | |a Plant biochemistry and molecular biology |c ed. by Peter J. Lea ... |
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Datensatz im Suchindex
| _version_ | 1804126958895759360 |
|---|---|
| adam_text | PLANT BIOCHEMISTRY
AND
MOLECULAR BIOLOGY
SECOND EDITION
Edited by
PETER J LEA
Department of Biological Sciences, Lancaster University, UK
and
RICHARD C LEEGOOD
Department of Animal and Plant Sciences, University of Sheffield, UK
JOHN WILEY amp; SONS
Chichester • New York • Weinheim • Brisbane • Singapore • Toronto
CONTENTS
Contributors xv
Preface to First Edition xvii
Preface to Second Edition xix
Abbreviations xxi
1 ENERGY PRODUCTION IN PLANT CELLS 1
J H Bryce and S A Hill
THE IMPORTANCE OF PHOTOSYNTHESIS 1
ADENOSINE TRIPHOSPHATE - ENERGY FOR BIOSYNTHESIS 2
SYNTHESIS OF ATP 3
ATPase 5
OVERVIEW OF PHOTOSYNTHESIS 5
PHOTOSYNTHETIC FORMATION OF ATP AND NADPH 5
Light Energy and Photosynthesis 5
The Pigments that Absorb Light 7
Photosystems and Electron Transport 9
GLYCOLYSIS AND THE OXIDATIVE PENTOSE PHOSPHATE PATHWAY 11
PPj-Fructose 6-Phosphate 1-Phosphotransferase (PFP) 14
GLUCONEOGENESIS 15
The Pathway of Gluconeogenesis and its Control 16
MITOCHONDRIA 17
Mitochondrial Transporters 18
Oxidation of Carbon Compounds 19
Electron Transport and Terminal Oxidases 20
Non-phosphorylating Electron Transport 24
CONTROL OF ENERGY PRODUCTION 26
REFERENCES 27
FURTHER READING 28
VI CONTENTS
2 PHOTOSYNTHESIS IN C3 PLANTS: THE BENSON-CALVIN CYCLE AND
PHOTORESPIRATION 29
R C Leegood
THE BENSON-CALVIN CYCLE 29
Formulation of the Benson-Calvin Cycle 30
Regulation of the Benson-Calvin Cycle 31
REACTIONS OF THE BENSON-CALVIN CYCLE 33
Reactions Catalysed by Rubisco 33
Regulation of Rubisco 34
Reduction of Glycerate 3-Phosphate to Triose Phosphate 36
Regeneration of RuBP from Triose Phosphate 36
Fructose 1,6-bisphosphatase 36
Sedoheptulose 1,7-bisphosphatase 36
Ribulase 5-phosphate kinase 37
Control of Flux by Enzymes of the Benson-Calvin Cycle 37
TRANSPORT ACROSS THE CHLOROPLAST ENVELOPE 38
Properties of the Phosphate Translocator 39
Translocation of Other Metabolites Across the Chloroplast Envelope 41
PHOTORESPIRATION 42
Inhibition of Carbon Assimilation by Oxygen 42
The Origin of Glycollate 42
The Recovery of Glycollate 43
The Reassimilation of NH3 46
Measurement of Photorespiratory Fluxes 46
Post-illumination Carbon Dioxide Burst 46
Stimulation of Photosynthetic Carbon Assimilation by Decreased Oxygen 47
Efflux of Carbon Dioxide into Carbon Dioxide-free Air 47
Uptake of UC02 47
Uptake of ls02 47
Photorespiratory Mutants 47
Factors Affecting the Rate of Photorespiration 48
The Role of Photorespiration 48
REFERENCES 49
FURTHER READING 50
3 CARBON DIOXIDE-CONCENTRATING MECHANISMS: C4 PHOTOSYNTHESIS
AND CRASSULACEAN ACID METABOLISM 51
R C Leegood
C4 PLANTS: THE SUPPRESSION OF PHOTORESPIRATION 52
The Elucidation of the C4 Pathway 52
Investigations using ,4C02 52
Leaf Anatomy in C4 Plants 54
Enzyme Distribution Between the Cell Types 55
The Operation of the C4 System 56
Differences in the C4 Mechanism 56
Intercellular Transport in C4 Plants 58
Regulation of the C4 Pathway 60
Sucrose and Starch Synthesis in C4 Plants 62
CONTENTS vii
CRASSULACEAN ACID METABOLISM 64
The Evidence for Crassulacean Acid Metabolism 65
The Nocturnal Conversion of Carbohydrate to Malate 66
Transport of Malate into the Vacuole 67
The Decarboxylation of Malate 68
Gas Exchange in Crassulacean Acid Metabolism Plants 69
Phase I: Malic Acid Synthesis in the Dark 69
Phase 2: The Initial Period in the Light 69
Phase 3: Malic Acid Decarboxylation 69
Phase 4: Malate Decarboxylation Ceases, as does Glucan Accumulation 69
Regulation of Crassulacean Acid Metabolism 69
Crassulacean Acid Metabolism as a Survival Mechanism 71
C3-C4 INTERMEDIATES 71
THE AVAILABILITY OF CARBON DIOXIDE IN THE AQUATIC ENVIRONMENT 73
C3, C4 AND CRASSULACEAN ACID METABOLISM COMPARED 74
Response to Carbon Dioxide and the Carbon Dioxide Compensation Concentration 74
Responses to Light and Temperature and Quantum Yields for Carbon Dioxide
Fixation in C3 and C4 Plants 75
Water-use Efficiency 77
Carbon Isotope Discrimination 77
REFERENCES 78
FURTHER READING 78
4 CARBOHYDRATE BIOCHEMISTRY 81
C J Smith
INTRODUCTION 82
THE HEXOSE MONOPHOSPHATE JUNCTION AND SINK-SOURCE
RELATIONS IN THE CYTOPLASM 83
COMPARTMENTALIZATION OF CARBOHYDRATE METABOLISM 86
METABOLISM OF SUCROSE 87
Role 87
Synthesis 88
Sources of Carbon for Sucrose Synthesis 89
Sucrose Cleavage 89
Sucrose Storage 90
Regulation of Sucrose Metabolism in the Cytoplasm and Starch Metabolism in the
Chloroplast 92
Feedforward Control of Sucrose Synthesis 92
Feedback Control of Sucrose Synthesis 95
Regulation of Starch Synthesis in the Chloroplast 96
Sucrose Transport 96
General 96
Transport of Sucrose Between Leaf Cells 97
Phloem Loading 97
Phloem Unloading 98
STARCH 99
Structure and Occurrence 99
Synthesis 100
Vlll CONTENTS
ADP-Glucose Pyrophosphorylase 100
Starch Synthase (ADP-glucose: 1,4-ct-D-glucan 4-D-glucosyltransferase) 101
Branching Enzyme [ J ,4-ct-glucan: 1,4-ct-D-glucan 6-cc-D-( 1,4-ct-D-glucano)-transferase] 102
Sources of Carbon for Starch Synthesis 102
Regulation of Starch Synthesis in Storage Tissues 103
Breakdown 103
a-Amylase (1,4-ct-D-glucan 4-glucanohvdrolase) 104
P-Amylase (1,4-ct-D-glucan maltohydrolase) 104
ct-Glucosidase (a-D-glucoside hydrolase) 104
Debranching Enzyme (a-dextrin 6-glucanohydrolase) 104
a 1:4 Glucan Phosphorylase (1,4-ct-D-glucan: orthophosphate a-D-glucosyltransferase) 104
FRUCTANS 105
General 105
Structure 105
Inulins 106
Levans 106
Neokestose Series 106
Synthesis 106
Synthesis of 1-Kestotriose 107
Chain Elongation 107
Degradation 108
Fructan Accumulation 108
THE CELL WALL 108
General 108
Polysaccharides of the Wall 109
Polymers of the Matrix 109
Pec tic Polysaccharides 109
Hemicellulosic Polysaccharides 110
Cellulose 111
Structural Proteins 111
Phenolic Compounds 111
Structure of the Wall 112
Cell Wall Synthesis 113
Provision of Nucleoside Diphosphate Sugars 113
Synthesis of Cellulose 114
Synthesis of Non-cellulosic Polysaccharides 114
CARBOHYDRATE STRUCTURE AND NOMENCLATURE 115
Monosaccharide Structure 115
Stereoisomerism: D- and L-Sugars 115
Ring Formation and Anomers 115
Glycoside Formation 116
REFERENCES 117
FURTHER READING 118
5 PLANT LIPIDS - THEIR METABOLISM, FUNCTION AND UTILIZATION 119
D J Murphy
INTRODUCTION 119
STRUCTURE 120
CONTENTS ix
Fatty Acids 120
Glycerolipids 121
BIOSYNTHESIS 122
Generation of Acetyl-CoA 122
Fatty Acid Biosynthesis 122
Formation of Oleoyl-CoA 126
Modification of Oleoyl-CoA 126
Assembly of Membrane Lipids 127
Assembly of Storage Lipids 129
Assembly of Structural Lipids 129
MOBILIZATION 130
Membrane Lipid Turnover 130
Storage Lipid Mobilization 131
Membrane Lipid Mobilization 131
MEMBRANE ORGANIZATION AND FUNCTION 132
AGRICULTURAL AND BIOTECHNOLOGICAL USES OF PLANT LIPIDS 134
Oilseeds 134
UTILIZATION OF SEED OILS 134
Edible Products 134
Non-edible Oils 134
Designer Oilseed Crops 134
REFERENCES 134
FURTHER READING 134
6 NITROGEN FIXATION 137
R J Smith, P J Lea and J R Gallon
INTRODUCTION 137
Sources of Nitrogen: The Nitrogen Cycle 138
Nitrogen Fixation 138
The Organisms 139
NITROGENASE 141
Molybdenum Nitrogenase 141
Isolation and Properties 141
Reaction Mechanism 143
Mo-independent Nitrogenases 145
Integration with Metabolism 146
THE GENETICS OF NITROGEN FIXATION 147
PLANTS AND NITROGEN FIXATION 149
Diazotrophic Cyanobacteria 149
Oxygen 149
Energy and Reductant 149
Assimilation of Fixed Nitrogen 149
The Rhizobium-Legume Symbiosis 150
The Control of Nodulation 152
Oxygen 152
Energy and Reductant 153
Assimilation of Fixed Nitrogen 154
Other Nitrogen-fixing Associations 155
X CONTENTS
REGULATION 156
Regulation of Nitrogenase Activity 156
Genetic Regulation of Nitrogen Fixation 157
CONCLUDING REMARKS ~ 159
REFERENCES 160
FURTHER READING 162
7 NITROGEN METABOLISM 163
P J Lea
INTRODUCTION 163
THE UPTAKE OF NITRATE 164
NITRATE REDUCTION 165
NITRATE REDUCTASE 165
Structure 165
Genetics and Molecular Biology 165
Regulation 166
NITRITE REDUCTASE 167
Genetics and Molecular Biology 167
Regulation 167
AMMONIA ASSIMILATION 168
GLUTAMINE SYNTHETASE 168
Structure 169
Molecular Biology and Regulation 169
GLUTAMATE SYNTHASE^ 170
Structure 170
Molecular Biology and Regulation 170
AMINOTRANSFERASES 171
NITROGEN STORAGE AND TRANSPORT 172
Asparagine Metabolism 172
The Ureides 173
THE ROLE OF AMMONIA IN PLANT METABOLISM 174
AMINO ACID FAMILIES 176
THE ASPARTATE PATHWAY 176
Lysine Synthesis 176
Threonine Synthesis 178
Methionine Synthesis 179
Isoleucine Synthesis 180
Overall Regulation of Aspartate Pathway 181
The Overproduction of Lysine and Threonine 181
THE GLUTAMATE AMINO ACIDS 183
Proline 183
Arginine 183
y-Aminobutyrate 184
THE AROMATIC AMINO ACIDS 184
SULPHATE ASSIMILATION 186
Sulphate Reduction 186
Glutathione 187
PHOTOSYNTHESIS INVOLVES NITROGEN AND SULPHUR METABOLISM 188
CONTENTS Xi
REFERENCES 190
FURTHER READING 191
8 SECONDARY METABOLISM 193
R Edwards and J A Gatehouse
INTRODUCTION 193
OVERVIEW OF SECONDARY METABOLISM 194
Branch Point Enzymes 194
Oxygenases 197
Methyltransferases 197
Structural Elaboration 198
Glycosyltransferases and Vacuolar Storage 198
MAJOR CLASSES OF SECONDARY PRODUCTS 200
PHENOLIC METABOLITES 200
Lignin 201
Flavonoids 202
POLYKETIDES 204
ISOPRENOIDS 204
NITROGEN-CONTAINING SECONDARY METABOLITES 206
Alkaloids Derived from Amino Acids 207
Other Alkaloids 208
Glycosylated Nitrogen-containing Toxins 209
Non-protein Amino Acids 209
FUNCTIONS OF SECONDARY METABOLITES IN PLANTS 210
Role in Plant-Microbe Interactions 210
Role in Plant-Insect Interactions 213
Role in Plant-Vertebrate Interactions 215
Role in Plant-Plant Interactions 216
IMPORTANCE OF PLANT SECONDARY METABOLITES TO MAN 216
REFERENCES 218
FURTHER READING 218
9 PLANT PIGMENTS 219
GAF Hendry
INTRODUCTION 219
COLOUR 220
THE PIGMENTS 223
Perspective 223
Tetrapyrroles 224
Tetraterpenoids 227
Flavonoids 229
Quinoid Compounds 231
Indole Compounds 232
COMMERCIAL APPLICATIONS AND BIOTECHNOLOGY 233
PLANT PIGMENTS IN A BIOLOGICAL CONTEXT 233
ACKNOWLEDGEMENTS 233
REFERENCES 234
FURTHER READING 234
xii CONTENTS
10 GENOME ORGANIZATION, PROTEIN SYNTHESIS AND PROCESSING IN
PLANTS 235
M D Watson and D J Murphy
INTRODUCTION 235
NUCLEAR GENOME ORGANIZATION 236
PLASTID GENOME ORGANIZATION 237
MITOCHONDRIAL GENOME ORGANIZATION 243
PROTEIN SYNTHESIS 244
Nuclear-encoded Proteins 244
Initiation 244
Elongation and Release 245
Chloroplast and Mitochondrial Protein Synthesis 246
PROTEIN SORTING 248
Targeting to Plastids 248
Investigating Chloroplast Protein Import 248
Precursors of Chloroplast Proteins 250
Structure and Specificity of Transit Peptides 250
Targeting of Proteins to the Stroma 250
Targeting to the Envelope Membranes and the Intermembrane Space 251
Targeting to the Thylakoid 251
The Secretory and Vacuolar Pathways 254
Secretion 255
Sorting to the Vacuole 255
Mitochondria 256
Microbodies 256
REFERENCES 258
FURTHER READING 258
11 REGULATION OF GENE EXPRESSION 261
A H Shirsat, J A Gatehouse and N J Robinson
INTRODUCTION 262
A REVIEW OF PLANT GENE STRUCTURE AND EXPRESSION 262
Genes are only a Fraction of Plant DNA 263
Role of Proximal Promoter Regulatory Sequences 263
Role of Distal Regulatory Sequences 263
Enhancers 265
Processing of RNA 266
Capping 266
Polyadenvlation 267
RNA Splicing 267
REGULATORY MECHANISMS: AN OVERVIEW 268
Control of Transcription 268
Measurement of Transcriptional Activity 269
Enabling Transcription 270
Activating Transcription 270
Post-transcriptional Control of Gene Expression 272
A Note about Regulation at the Level of Translation 273
Other Regulatory Mechanisms 273
CONTENTS xiii
Promoter-Reporter Gene Fusions 273
Luciferase 274
P-Glucuronidase 274
Aequorin 274
Green Fluorescent Protein 275
Signal Transduction Pathways 275
Primary Sensors 276
Secondary Messengers 277
REGULATORY MECHANISMS: SOME SELECTED EXAMPLES 277
The Perception of Ethylene 277
etrl: An Ethylene Sensor 278
Ctrl: One of the Messengers 279
Responses to Pathogens 280
Induced Genes 280
The Plant Cell Wall 281
The Hypersensitive Response and the Isolation of Resistance Genes 282
REFERENCES 284
FURTHER READING 285
12 MOLECULAR CONTROL OF DEVELOPMENT 287
J A Bryant and A C Cuming
INTRODUCTION 287
EXPERIMENTAL METHODS IN THE STUDY OF DEVELOPMENT 289
Map-based Cloning 290
Gene Tagging: Transposons 293
Gene Tagging: T-DNA 296
Isolation of Tagged Genes 297
Reverse Genetics 298
GENE REGULATION IN PLANT DEVELOPMENT 300
FERTILIZATION AND SEED DEVELOPMENT 300
Introduction 300
Fertilization 303
Gametophytic Incompatibility 304
Sporophytic Incompatibility 305
Barriers to Interspecies Pollination 308
Embryogenesis 308
Embryonic Maturation 311
FRUIT DEVELOPMENT AND RIPENING 313
Introduction 313
Climacteric and Non-climacteric Fruit 314
Ripening in Tomato 314
GERMINATION 320
Introduction 320
Hormones and Germination 320
Mobilization of Reserves in Cereal Grains 321
Regulation of Gene Expression by Gibberellic Acid 321
FLORAL DEVELOPMENT 325
Homoeotic Mutations in Floral Development 327
xiv CONTENTS
Molecular Analysis of Homoeotic Genes 330
FURTHER READING 332
13 CELL CULTURE, TRANSFORMATION AND GENE TECHNOLOGY 335
R Walden
INTRODUCTION 335
PLANT CELL AND TISSUE CULTURE 336
Protoplast Isolation 336
Transient Expression Assays 336
DMGT and Stable Transformation 338
Plant Regeneration 338
AGROBACTERIUM-MEDIATED TRANSFORMATION 339
The Biology of the Agrobacterium-P sint Interaction 339
Transformation using Agrobacterium 340
VIRAL VECTORS ~ 342
USING TRANSGENIC PLANTS 343
Promoter Analysis and Controlling Gene Expression in Transgenic Plants 343
Knocking Out Gene Activity by Antisense Expression and Co-suppression 344
Biochemical Function of Gene Products 345
Gene Tagging 345
ENGINEERING POTENTIALLY USEFUL AGRONOMIC TRAITS 346
Herbicide Tolerance 346
Resistance to Insects 348
Engineering Viral Resistance 348
Resistance to Fungal and Bacterial Pathogens 349
MANIPULATING PLANT PRODUCTS ~ 349
Carbohydrates 349
Fats and Oils 350
ENGINEERING PLANTS TO SYNTHESIZE NOVEL PRODUCTS 351
CONCLUSIONS AND FUTURE PROSPECTS 352
REFERENCES 353
FURTHER READING 355
INDEX 357
|
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| id | DE-604.BV012332518 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:25:44Z |
| institution | BVB |
| isbn | 0471976822 0471976830 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008362067 |
| oclc_num | 39384947 |
| open_access_boolean | |
| owner | DE-M49 DE-BY-TUM DE-703 |
| owner_facet | DE-M49 DE-BY-TUM DE-703 |
| physical | XVI, 364 S. Ill., graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Wiley |
| record_format | marc |
| spelling | Plant biochemistry and molecular biology ed. by Peter J. Lea ... 2. ed. Chichester [u.a.] Wiley 1999 XVI, 364 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biochemie gtt Biologie moléculaire végétale Chimie végétale Moleculaire biologie gtt Planten gtt Botanical chemistry Molecular Biology Plant Physiological Phenomena Plant molecular biology Plants chemistry Phytochemie (DE-588)4174632-6 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf Stoffwechsel (DE-588)4057699-1 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Biochemie (DE-588)4006777-4 gnd rswk-swf Pflanzen (DE-588)4045539-7 s Molekularbiologie (DE-588)4039983-7 s DE-604 Biochemie (DE-588)4006777-4 s Stoffwechsel (DE-588)4057699-1 s Phytochemie (DE-588)4174632-6 s 1\p DE-604 Lea, Peter J. 1944- Sonstige (DE-588)12248956X oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008362067&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Plant biochemistry and molecular biology Biochemie gtt Biologie moléculaire végétale Chimie végétale Moleculaire biologie gtt Planten gtt Botanical chemistry Molecular Biology Plant Physiological Phenomena Plant molecular biology Plants chemistry Phytochemie (DE-588)4174632-6 gnd Pflanzen (DE-588)4045539-7 gnd Stoffwechsel (DE-588)4057699-1 gnd Molekularbiologie (DE-588)4039983-7 gnd Biochemie (DE-588)4006777-4 gnd |
| subject_GND | (DE-588)4174632-6 (DE-588)4045539-7 (DE-588)4057699-1 (DE-588)4039983-7 (DE-588)4006777-4 |
| title | Plant biochemistry and molecular biology |
| title_auth | Plant biochemistry and molecular biology |
| title_exact_search | Plant biochemistry and molecular biology |
| title_full | Plant biochemistry and molecular biology ed. by Peter J. Lea ... |
| title_fullStr | Plant biochemistry and molecular biology ed. by Peter J. Lea ... |
| title_full_unstemmed | Plant biochemistry and molecular biology ed. by Peter J. Lea ... |
| title_short | Plant biochemistry and molecular biology |
| title_sort | plant biochemistry and molecular biology |
| topic | Biochemie gtt Biologie moléculaire végétale Chimie végétale Moleculaire biologie gtt Planten gtt Botanical chemistry Molecular Biology Plant Physiological Phenomena Plant molecular biology Plants chemistry Phytochemie (DE-588)4174632-6 gnd Pflanzen (DE-588)4045539-7 gnd Stoffwechsel (DE-588)4057699-1 gnd Molekularbiologie (DE-588)4039983-7 gnd Biochemie (DE-588)4006777-4 gnd |
| topic_facet | Biochemie Biologie moléculaire végétale Chimie végétale Moleculaire biologie Planten Botanical chemistry Molecular Biology Plant Physiological Phenomena Plant molecular biology Plants chemistry Phytochemie Pflanzen Stoffwechsel Molekularbiologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008362067&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT leapeterj plantbiochemistryandmolecularbiology |