Biological specimen preparation for transmission electron microscopy:
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
London
Portland Press
1998
|
| Schriftenreihe: | Practical methods in electron microscopy
17 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXI, 326 S. Ill., graph. Darst. |
| ISBN: | 1855780607 1855780615 |
Internformat
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| 100 | 1 | |a Glauert, Audrey M. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Biological specimen preparation for transmission electron microscopy |c Audrey M. Glauert ; Peter R. Lewis |
| 264 | 1 | |a London |b Portland Press |c 1998 | |
| 300 | |a XXI, 326 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Practical methods in electron microscopy |v 17 | |
| 650 | 7 | |a Elektronenmicroscopie |2 gtt | |
| 650 | 7 | |a Preparaten |2 gtt | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Mounting of microscope specimens | |
| 650 | 4 | |a Transmission electron microscopy |x Technique | |
| 650 | 0 | 7 | |a Biologisches Material |0 (DE-588)4145633-6 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Biologisches Material |0 (DE-588)4145633-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Lewis, Peter Rhys |e Verfasser |4 aut | |
| 830 | 0 | |a Practical methods in electron microscopy |v 17 |w (DE-604)BV001891054 |9 17 | |
| 856 | 4 | 2 | |m GBV Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008266579&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
Datensatz im Suchindex
| _version_ | 1805083633510252544 |
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| adam_text |
BIOLOGICAL SPECIMEN PREPARATION FOR TRANSMISSION ELECTRON MICROSCOPY
AUDREY M. GLAUERT CLARE HALL UNIVERSITY OF CAMBRIDGE U.K. PETER R. LEWIS
PHYSIOLOGICAL LABORATORY UNIVERSITY OF CAMBRIDGE U.K. PORTLAND PRESS
LONDON CONTENTS XIII CONTENTS EDITOR'S PREFACE TO THE SERIES VII
AUTHORS' PREFACE AND ACKNOWLEDGEMENTS IX I AN INTRODUCTION TO FIXATION
AND EMBEDDING PROCEDURES I AND THEIR SAFE USE IN THE LABORATORY 1.1 THE
SCOPE OF THIS BOOK I 1.2 CRITERIA FOR THE GOOD PRESERVATION OF
UKRASTRUCTURE 4 1.3 ARTEFACTS IN ELECTRON MICROGRAPHS 6 1.4 SAFETY
PRECAUTIONS IN THE ELECTRON MICROSCOPE LABORATORY 9 1.4.1 GENERAL SAFETY
PRECAUTIONS 9 1.4.2 HAZARDS FROM ELECTRICAL EQUIPMENT AND FIRE 10 1.4.3
SAFE PROCEDURES FOR HANDLING ANIMALS II 1.4.4 DANGERS FROM SHARP
IMPLEMENTS 12 1.4.5 SAFE PROCEDURES WITH CHEMICALS 12 1.4.5A LABELLING
FOR TOXIDTY AND FLAMMABILIVY 12 1.4.5B THE SAFE HANDLING OF CHEMICALS 15
1.4.5C STORAGE AND DISPOSAL OF CHEMICALS 16 1.4.6 HAZARDS WITH LIQUID
NITROGEN II 1.4.7 FIRST-AID IN THE LABORATORY 18 2 FIXATIVES 2 F 2. / AN
INTRODUCTION TO FIXATION 21 2.1.1 THE DEVELOPMENT OF FIXATIVES FOR
ELECTRON MICROSCOPY 22 2.1.2 THE CHEMISTRY AND PHYSICS OF FIXATION 24
2.1.3 THE PENETRATION OF FIXATIVES 25 2.1.4 THE CHOICE OF ALDEHYDE
FIXATIVE 27 2.2 THE SAFE PREPARATION AND DISPOSAL OF FIXATIVES 29 2.2.1
PREPARATION OF A FIXATIVE 30 2.2.2 DISPOSAL OF FIXATIVES 32 2.2.3
SPILLAGES 33 XIV CONTENTS 2.3 VEHICLES FOR FIXATIVES 33 2.3.1 THE CHOICE
OF BUFFER 34 2.3.2 OSMOLARITY " 35 2.3.3 THE PREPARATION OF BUFFERS 37
2.4 GLUTARALDEHYDE FIXATIVES 38 2.4.1 THE EFFECTS OF GLUTARALDEHYDE ON
CELLS AND TISSUES 40 2.4.1 A REACTIONS OF GLUTARALDEHYDE WITH PROTEINS
40 2.4.1B REACTIONS OF GLUTARALDEHYDE WITH OTHER CELL CONSTITUENTS 44
2.4.1C THE EFFECTS OF THE OSMOLARITY OF GLUTARALDEHYDE FIXATIVES 44
2.4.2 THE PREPARATION OF GLUTARALDEHYDE FIXATIVES 45 2.5 FORMALDEHYDE
FIXATIVES 46 2.5.1 THE EFFECTS OF FORMALDEHYDE ON CELLS AND TISSUES 47
2.5.2 THE PREPARATION OF FORMALDEHYDE FIXATIVES 50 2.5.2A A
PHOSPHATE-BUFFERED FORMALDEHYDE FIXATIVE 51 2.5.2B A CACODYLATE-BUFFERED
FORMALDEHYDE FIXATIVE 51 2.5.2C A PIPES-BUFFERED FORMALDEHYDE FIXATIVE
52 2.5.3 GLUTARALDEHYDE-FORMALDEHYDE FIXATIVES 52 2.5.3A THE PREPARATION
OF A GLUTARALDEHYDE-FORMALDEHYDE FIXATIVE 53 2.5.4 ADDITIVES TO ALDEHYDE
FIXATIVES 53 2.6 OSMIUM TETROXIDE FIXATIVES 55 2.6.1 THE EFFECTS OF
OSMIUM TETROXIDE FIXATION 56 2.6.2 ROUTINE POST-FIXATION WITH OSMIUM
TETROXIDE 58 2.6.3 OSMIUM TETROXIDE AND COMPLEX CYANIDES 60 2.6.4 AMINE
COMPLEXES WITH OSMIUM 61 2.6.5 OTHER OSMIUM 2.7 URANYL ACETATE 63 2.8
OTHER FIXATIVES 66 2.8.1 ACROLEIN 66 2.8.2 TANNICADD 67 2.8.3 POTASSIUM
DICHROMATE 70 2.8.4 OTHER MISCELLANEOUS FIXATIVES 71 3 FIXATION METHODS
77 3.1 THE CHOICE OF FIXATION METHOD 77 3.2 THE CHOICE OF FIXATIVES 81
CONTENTS XV 3.3 STANDARD FIXATION METHODS 83 3.3.1 PRIMARY FIXATION 83
3.3.2 WASHING AND STORAGE " 83 3.3.3 SECONDARY AND TERTIARY FIXATION 85
3.4 FIXATION BY IMMERSION 85 3.4.1 A STANDARD SCHEDULE FOR FIXATION BY
IMMERSION 88 3.5 THE PRIMARY FIXATION OF ANIMAL TISSUES 89 3.5.1
IMMERSION FIXATION OF ANIMAL TISSUES 89 3.5.1A PREPARATION OF THIN
TISSUE SLICES 90 3.5.1B ORIENTATION OF TISSUE PIECES AND SLICES 92 3.5.2
SURFACE APPLICATION OF A FIXATIVE 93 3.5.3 FIXATION BY INJECTION 94 3.6
THE FIXATION OF INVERTEBRATES 94 3.7 THE FIXATION OF BOTANICAL SPECIMENS
95 3.7.1 FIXATION PROCEDURES 95 3.7.2 THE CHOICE OF FIXATIVES 97 3.7.3 A
STANDARD FIXATION PROCEDURE FOR PLANT TISSUES 98 3.8 FIXATION BY
VASCULAR PERFUSION 98 3.8.1 AN OUTLINE OF PERFUSION FIXATION 99 3.8.2
THE CHOICE OF ANAESTHETIC 99 3.8.3 THE CHOICE OF PERFUSION FLUIDS 101
3.8.4 APPARATUS FOR PERFUSION FIXATION 102 3.8.5 THE ROUTINE PERFUSION
OF MAMMALS 104 3.8.5A A STANDARD PERFUSION TECHNIQUE FOR SMALL MAMMALS
105 3.8.5B PERFUSION TECHNIQUES FOR LARGER ANIMALS 106 3.8.6 PERFUSION
OF HEART TISSUES 106 3.8.7 PERFUSION OF THE KIDNEYS 107 3.8.8 FIXATION
OF LUNG TISSUE 107 3.8.9 PORTAL PERFUSION OF LIVER TISSUE 108 3.8.10 THE
PERFUSION OF FISH 108 3.9 FIXATION OF ORGAN AND MONOLAYER CELL CULTURES
109 3.10 FIXATION OF ISOLATED CELLS III 3.10.1 FIXATION OF CELLS IN
SUSPENSION IN MEDIUM 112 3.10.2 FIXATION OF CELLS AND ORGANELLES IN A
PELLET 114 3.10.3 ENCAPSULATING METHODS FOR PELLETS 115 3.10.4
COLLECTING CELLS AND VIRUSES ON MILLIPORE FILTERS 118 3.11 FIXATION BY
MICROWAVE IRRADIATION 118 XVI CONTENTS 3.12 FIXATION METHODS FOR
SPECIALIZED TECHNIQUES 119 3.12.1 FIXATION FOR GENERAL CYTOCHEMICAL
TECHNIQUES 120 3.12.2 FIXATION FOR ENZYME CYTOCHEMICAL TECHNIQUES 123
3.12.3 FIXATION FOR IMMUNOCYTOCHEMICAL TECHNIQUES 125 4 DEHYDRATION
METHODS 129 4.1 CHEMICAL AND MORPHOLOGICAL EFFECTS OF DEHYDRATION 129
4.1.1 DIMENSIONAL CHANGES DURING DEHYDRATION 130 4.1.2 THE RETENTION OF
LIPIDS 131 4.2 SAFETY PROCEDURES 134 4.3 DEHYDRATING AGENTS AND
INTERMEDIATE SOLVENTS 134 4.3.1 THE STANDARD DEHYDRATING AGENTS AND
INTERMEDIATE SOLVENTS 134 4.3.2 ALTERNATIVE DEHYDRATING AGENTS AND
INTERMEDIATE SOLVENTS 135 4.4 DEHYDRATION SCHEDULES 136 4.4.1 A STANDARD
DEHYDRATION SCHEDULE 137 4.4.2 ALTERNATIVE DEHYDRATION SCHEDULES 138
4.4.3 RAPID DEHYDRATION 139 4.4.4 PARTIAL DEHYDRATION 140 4.5
DEHYDRATION BY FREEZE-SUBSTITIITION 142 4.5.1 SOLVENTS FOR
FREEZE-SUBSTITUTION 142 4.5.2 FIXATIVES FOR FREEZE-SUBSTITUTION 143
4.5.3 THE FREEZE-SUBSTITUTION PROCEDURE 144 EMBEDDING METHODS 147 5.1
THE ATTRIBUTES OF EMBEDDING MEDIA 147 147 149 149 150 151 152 152 153
5.2 THE SAFE HANDLING OF EMBEDDING MEDIA 154 THE ATTRIBUTES OF EMBEDDING
MEDIA 5.1.1 5.1.2 5.1.3 5.1.4 5.1.5 5.1.6 5.1.7 5.1.8 TOXICITY
AVAILABILITY UNIFORMITY OF INFILTRATION INTERACTIONS WITH THE SPECIMEN
UNIFORMITY OF HARDENING EASE OF SECTIONING SECTION STAINING STABILITY IN
THE ELECTRON BEAM CONTENTS XVII 5.3 EMBEDDING PROCEDURES 155 5.3.1
INFILTRATION OF EMBEDDING MEDIA 156 5.3.1A STANDARD INFILTRATION METHODS
156 5.3.1B CONTINUOUS INFILTRATION 156 5.3.1C VACUUM INFILTRATION 157
5.3.2 EMBEDDING MOULDS 158 5.3.3 CURING AND POLYMERIZATION OF EMBEDDING
MEDIA 161 5.3.3A EMBEDDING IN CAPSULES 161 5.3.3B FLAT EMBEDDING 163
5.3.3C METHODS OF CURING AND POLYMERIZATION 164 5.3.3D ULTRA-VIOLET AND
MICROWAVE IRRADIATION 164 5.4 EMBEDDING METHODS FOR CELL MONOLAYERS 165
5.4.1 REMOVAL OF HARDENED BLOCKS FROM THE SUBSTRATE 166 5.4.2 MONOLAYER
EMBEDDED AND SECTIONED ON THE SUBSTRATE 169 6 EMBEDDING IN EPOXY RESINS
175 6.1 THE COMPONENTS OF EPOXY RESIN EMBEDDING MEDIA 175 6.1.1 EPOXY
RESINS 176 6.1.2 HARDENERS FOR EPOXY RESINS 179 6.1.3 ACCELERATORS FOR
EPOXY RESINS 181 6.1.4 ADDITIVES FOR EPOXY RESIN EMBEDDING MEDIA 182
6.1.4A PLASTIDZERS 183 6.1.4B REACTIVE FLEXIBILIZERS 183 6.1.5 THE
ANHYDRIDE:EPOXIDE RATIO 186 6.1.6 CURING TEMPERATURES FOR EPOXY RESINS
187 6.2 EPOXY RESIN EMBEDDING MEDIA 188 6.2.1 THE DEVELOPMENT OFEPOXY
RESIN EMBEDDING MEDIA 188 6.2.2 THE AROMATIC ARALDITE EPOXY RESINS 189
6.2.3 ARALDITE EMBEDDING MEDIA 191 6.2.3A THE STANDARD EMBEDDING MEDIA 1
9 / 6.2.3B ADJUSTMENT OF THE CONTENT OFDIBUTYL PHTHALATE 192 6.2.3C
ADJUSTMENT OF THE HARDNESS OF THE FINAL BLOCK 192 6.2.4 ALTERNATIVE
AROMATIC EPOXY RESINS 193 6.2.4A DGEBA RESINS CONTAINING BGE 193 6.2.4B
DGEBA RESINS CONTAINING BDE 194 6.2.5 THE ALIPHATIC 'EPON' EPOXY RESINS
195 XVIII CONTENTS 6.2.6 'EPON' EMBEDDING MEDIA 196 6.2.6A LUFT'S A AND
B MIX 'EPON' EMBEDDING MEDIUM 196 6.2.6B SINGLE-MIX'EPON'EMBEDDING MEDIA
197 6.2.6C 'EPON' EMBEDDING MEDIA WITH LOWER VISCOSITIES 198 6.2.7
ARALDITE/'EPON' EMBEDDING MEDIA 199 6.2.8 STORAGE AND MIXING OF THE
COMPONENTS OF THE MEDIA 200 6.2.8A PREPARATION OFEPOXY RESIN EMBEDDING
MEDIA 201 6.2.8B STORAGE OFEPOXY RESIN EMBEDDING MEDIA 202 6.2.9
EMBEDDING METHODS FOR EPOXY RESINS 202 6.2.9A THE STANDARD PROCEDURE FOR
INFILTRATION WITH EPOXY RESINS 202 6.2.9B MODIFIED INFILTRATION
PROCEDURES 204 6.2.9C INFILTRATION AFTER DEHYDRATION BY
FREEZE-SUBSTITUTION 206 6.2.9D THE CURING OFEPOXY RESINS 207 6.2.9E
RAPID INFILTRATION AND CURING OFEPOXY RESINS 208 6.3 LOW VISCOSITY EPOXY
RESIN EMBEDDING MEDIA 209 6.3.1 SPURR'S EMBEDDING MEDIUM 210 6.3.1A
PREPARATION OF SPURR'S EMBEDDING MEDIUM 211 6.3.1B INFILTRATION
SCHEDULES FOR SPURR'S MEDIUM 212 6.3.1 C EMBEDDING PROCEDURE FOR SPURR'S
MEDIUM 214 6.3.2 ULTRA-LOW VISCOSITY EPOXY RESIN EMBEDDING MEDIA 215
6.3.3 QUETOL 651 EPOXY RESIN EMBEDDING MEDIUM 215 6.3.4 PROPERTIES OF
LOW VISCOSITY EPOXY RESIN EMBEDDING MEDIA 217 6.4 WATER-MISCIBLE EPOXY
RESINS 218 6.4.1 AQUON 219 6.4.2 DURCUPAN 219 - 6.4.2A DEHYDRATION AND
EMBEDDING IN DURCUPAN 219 6.4.2B CONVENTIONAL EMBEDDING AFTER DURCUPAN
DEHYDRATION 220 6.4.3 AQUEMBED 221 7 EMBEDDING IN ACRYLIC RESINS 225 7.1
ACRYLIC RESINS FOR ELECTRON MICROSCOPY 225 7.1.1 THE PROPERTIES OF
ACRYLIC RESINS 225 7.1.2 THE POLYMERIZATION OF ACRYLIC RESINS 227 7.1.3
STABILITY IN THE ELECTRON BEAM 228 7.1.4 THE DEVELOPMENT OF ACRYLIC
RESIN EMBEDDING MEDIA 228 CONTENTS XIX 7.1.5 EMBEDDING METHODS FOR
ACRYLIC RESINS 231 7.1.5A SAFE HANDLING OF ACRYLIC RESINS 231 7.1.5B
STORAGE AND MIXING OF ACRYLIC RESINS 232 7.1.5C INFILTRATION AND
POLYMERIZATION OF ACRYLIC RESINS 232 7.2 GLYCOL METHACRYLATE FOR LIGHT
MICROSCOPY 233 7.2.1 GLYCOL METHACRYLATE 233 7.2.2 POLYMERIZATION OF
GLYCOL METHACRYLATE 234 7.2.3 EMBEDDING IN GLYCOL METHACRYLATE FOR LIGHT
MICROSCOPY 236 7.2.4 EMBEDDING IN GLYCOL METHACRYLATE AT LOW
TEMPERATURES 239 7.3 THE LONDON RESINS, LR WHITE AND LR GOLD 240 7.3.1
LR WHITE 243 7.3.2 VNCATALYSED' LR WHITE 244 7.3.3 PROCESSING SCHEDULES
FOR LR WHITE 244 7.3.3A POLYMERIZATION BY HEAT 245 7.3.3B POLYMERIZATION
AT 0C 246 7.3.3C POLYMERIZATION BY UV IRRADIATION BELOW 0C 247 7.3.4
LR GOLD 248 8 THE LOW/CRY/ RESINS AND EMBEDDING AT LOW TEMPERATURES 25 /
8./ THE ADVANTAGES OF LOW TEMPERATURE EMBEDDING 251 8.2 THE LOWICRYL
RESINS 251 8.3 THE POLAR LOWICRYL RESINS 254 8.3.1 LOWICRYL K4M 254
8.3.2 LOWICRYL KL IM 255 8.4 THE NON-POLAR LOWICRYL RESINS 256 8.4.1
LOWICRYL HM20 256 8.4.2 LOWICRYL HM23 257 8.5 THE PREPARATION OF
LOWICRYL EMBEDDING MEDIA 258 8.6 APPARATUS FOR DEHYDRATION AND EMBEDDING
AT LOW TEMPERATURES 259 8.6.1 COOLING BATHS AND COLD CABINETS 259 8.6.2
COMMERCIAL UNITS 260 8.7 INFILTRATION SCHEDULES FOR LOWICRYL RESINS 262
8.7.1 INFILTRATION AFTER DEHYDRATION BY THE PLT METHOD 263 8.7.2
INFILTRATION AFTER DEHYDRATION BY FREEZE-SUBSTITUTION 265 8.7.3
INFILTRATION AFTER FREEZE-DRYING 267 XX CONTENTS 8.8 POLYMERIZATION OF
LOWICRYL RESINS AT LOW TEMPERATURES 269 8.8.1 REQUIREMENTS FOR
POLYMERIZATION AT LOW TEMPERATURES 269 8.8.2 PROCEDURES FOR
POLYMERIZATION AT LOW TEMPERATURES 2 71 9 OTHER EMBEDDING MEDIA 279 9.1
POLYESTER RESINS 279 9.1.1 VESTOPAL W EMBEDDING MEDIUM 279 9.1.2
EMBEDDING SCHEDULES FOR VESTOPAL W 280 9.2 MELAMINE RESINS 281 9.2.1 THE
PROPERTIES OF MELAMINE RESINS 281 9.2.2 NANOPLAST FB 101 EMBEDDING
MEDIUM 283 9.2.3 EMBEDDING SCHEDULE FOR NANOPLAST FB 101 285 9.2.4 LOW
TEMPERATURE EMBEDDING IN NANOPLAST 286 9.3 WATER-MISDBLE EMBEDDING MEDIA
287 9.4 POLYETHYLENE GLYCOL AND OTHER REVERSIBLE EMBEDDING MEDIA 288 10
PROCESSING SCHEDULES 293 10.1 THE STANDARD SCHEDULE FOR UKRASTRUCTURAL
STUDIES 293 10.2 SCHEDULES FOR GENERAL CYTOCHEMISTRY 294 10.2.1 A
SCHEDULE FOR STAINING SECTIONS WITH COLLOIDAL IRON 295 10.2.2 A SCHEDULE
FOR STAINING TISSUES WITH COLLOIDAL THORIUM DIOXIDE 295 10.2.3 A
SPECIFIC STAINING SCHEDULE FOR BIOGENIC AMINES 297 10.3 SCHEDULES FOR
ENZYME CYTOCHEMISTRY 297 10.3.1 A ROUTINE SCHEDULE FOR THE LOCALIZATION
OF ENZYMES 298 10.3.2 A SPECIFIC SCHEDULE FOR 5 '-NUCLEOTIDASE 299 10.4
SCHEDULES FOR IMMUNOCYTOCHEMISTRY 300 10.4.1 PRE-EMBEDDING
IMMUNOLABELLING 301 10.4.2 POST-EMBEDDING IMMUNOLABELLING 301 10.4.2A
IMMUNOLABELLING OF ACRYLIC RESIN SECTIONS 302 10.4.2B IMMUNOLABELLING OF
EPOXY RESIN SECTIONS 303 10.5 SCHEDULES FOR RAPID PROCESSING 303 10.5.1
A RAPID PROCESSING SCHEDULE FOR SMALL SPECIMENS 303 10.5.2 A SCHEDULE
FOR RAPID PROCESSING OF BIOPSIES 305 10.5.3 RAPID PROCESSING BY
MICROWAVE IRRADIATION 305 CONTENTS XXI 10.6 SCHEDULES FOR REPROCESSING
WAX-EMBEDDED MATERIAL 307 10.6.1 A SCHEDULE FOR REMOVAL OF PARAFFIN WAX
WITH XYLENE 307 10.6.2 A SCHEDULE FOR REMOVAL OF PARAFFIN WAX WITH
INHIBISOL 307 10.7 A SCHEDULE FOR THE DECALCIFICATION OF MINERALIZED
TISSUE 308 10.8 SCHEDULES FOR EMBEDDING HARD MATERIALS 309 10.8.1 A
SCHEDULE FOR EMBEDDING HARD BIOLOGICAL SPECIMENS 309 10.8.2 A SCHEDULE
FOR EMBEDDING HARD MATERIALS IN NANOPLAST 310 APPENDIX: LIST OF
SUPPLIERS 313 SUBJECT INDEX 319 |
| any_adam_object | 1 |
| author | Glauert, Audrey M. Lewis, Peter Rhys |
| author_facet | Glauert, Audrey M. Lewis, Peter Rhys |
| author_role | aut aut |
| author_sort | Glauert, Audrey M. |
| author_variant | a m g am amg p r l pr prl |
| building | Verbundindex |
| bvnumber | BV012199740 |
| callnumber-first | Q - Science |
| callnumber-label | QH239 |
| callnumber-raw | QH239 |
| callnumber-search | QH239 |
| callnumber-sort | QH 3239 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 3100 |
| classification_tum | CHE 264f BIO 040f |
| ctrlnum | (OCoLC)632791748 (DE-599)BVBBV012199740 |
| dewey-full | 570/.28/27 570.2825 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 570 - Biology |
| dewey-raw | 570/.28/27 570.2825 |
| dewey-search | 570/.28/27 570.2825 |
| dewey-sort | 3570 228 227 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| format | Book |
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| id | DE-604.BV012199740 |
| illustrated | Illustrated |
| indexdate | 2024-07-20T07:51:39Z |
| institution | BVB |
| isbn | 1855780607 1855780615 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008266579 |
| oclc_num | 632791748 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-20 DE-91G DE-BY-TUM DE-B16 |
| owner_facet | DE-355 DE-BY-UBR DE-20 DE-91G DE-BY-TUM DE-B16 |
| physical | XXI, 326 S. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
| publisher | Portland Press |
| record_format | marc |
| series | Practical methods in electron microscopy |
| series2 | Practical methods in electron microscopy |
| spelling | Glauert, Audrey M. Verfasser aut Biological specimen preparation for transmission electron microscopy Audrey M. Glauert ; Peter R. Lewis London Portland Press 1998 XXI, 326 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Practical methods in electron microscopy 17 Elektronenmicroscopie gtt Preparaten gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Mounting of microscope specimens Transmission electron microscopy Technique Biologisches Material (DE-588)4145633-6 gnd rswk-swf Raster-Transmissions-Elektronenmikroskopie (DE-588)4320991-9 gnd rswk-swf Raster-Transmissions-Elektronenmikroskopie (DE-588)4320991-9 s Biologisches Material (DE-588)4145633-6 s DE-604 Lewis, Peter Rhys Verfasser aut Practical methods in electron microscopy 17 (DE-604)BV001891054 17 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008266579&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Glauert, Audrey M. Lewis, Peter Rhys Biological specimen preparation for transmission electron microscopy Practical methods in electron microscopy Elektronenmicroscopie gtt Preparaten gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Mounting of microscope specimens Transmission electron microscopy Technique Biologisches Material (DE-588)4145633-6 gnd Raster-Transmissions-Elektronenmikroskopie (DE-588)4320991-9 gnd |
| subject_GND | (DE-588)4145633-6 (DE-588)4320991-9 |
| title | Biological specimen preparation for transmission electron microscopy |
| title_auth | Biological specimen preparation for transmission electron microscopy |
| title_exact_search | Biological specimen preparation for transmission electron microscopy |
| title_full | Biological specimen preparation for transmission electron microscopy Audrey M. Glauert ; Peter R. Lewis |
| title_fullStr | Biological specimen preparation for transmission electron microscopy Audrey M. Glauert ; Peter R. Lewis |
| title_full_unstemmed | Biological specimen preparation for transmission electron microscopy Audrey M. Glauert ; Peter R. Lewis |
| title_short | Biological specimen preparation for transmission electron microscopy |
| title_sort | biological specimen preparation for transmission electron microscopy |
| topic | Elektronenmicroscopie gtt Preparaten gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Mounting of microscope specimens Transmission electron microscopy Technique Biologisches Material (DE-588)4145633-6 gnd Raster-Transmissions-Elektronenmikroskopie (DE-588)4320991-9 gnd |
| topic_facet | Elektronenmicroscopie Preparaten Wetenschappelijke technieken Wissenschaftliches Arbeiten Mounting of microscope specimens Transmission electron microscopy Technique Biologisches Material Raster-Transmissions-Elektronenmikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008266579&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV001891054 |
| work_keys_str_mv | AT glauertaudreym biologicalspecimenpreparationfortransmissionelectronmicroscopy AT lewispeterrhys biologicalspecimenpreparationfortransmissionelectronmicroscopy |