Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
1997
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 106 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV012192035 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 980929s1997 gw ad|| m||| 00||| ger d | ||
| 016 | 7 | |a 954385209 |2 DE-101 | |
| 035 | |a (OCoLC)51616732 | ||
| 035 | |a (DE-599)BVBBV012192035 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a ger | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 |a DE-83 |a DE-11 |a DE-188 | ||
| 100 | 1 | |a Timmermann, Stefanie Alexandra |d 1967- |e Verfasser |0 (DE-588)12042052X |4 aut | |
| 245 | 1 | 0 | |a Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis |c vorgelegt von Stefanie Alexandra Timmermann |
| 264 | 1 | |c 1997 | |
| 300 | |a 106 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Köln, Univ., Diss., 1997 | ||
| 650 | 0 | 7 | |a Retinoblastom |0 (DE-588)4177902-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mundhöhlenkrebs |0 (DE-588)4040733-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Carcinogenese |0 (DE-588)4069853-1 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Mundhöhlenkrebs |0 (DE-588)4040733-0 |D s |
| 689 | 0 | 1 | |a Carcinogenese |0 (DE-588)4069853-1 |D s |
| 689 | 0 | 2 | |a Retinoblastom |0 (DE-588)4177902-2 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008262338&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008262338 | |
Datensatz im Suchindex
| _version_ | 1807773355050270720 |
|---|---|
| adam_text |
CONTENTS
ABBREVIATIONS
1
1.
INTRODUCTION
2
1.1
1.1.1
1.1.2
1.1.3
ORAL
CANCER
2
EPIDEMIOLOGY
2
ETIOLOGY
2
MOLECULAR
CHANGES
3
1.2
1.2.1
1.2.2
THE
P53
PATHWAY
4
OVERVIEW
-YY
4
THE
P53
PATHWAY
AND
CANCER
5
1.3
1.3.1
1.3.2
1.3.3
1.3.4
1.3.5
2.
MAL
THE
PRB
PATHWAY
6
OVERVIEW
6
REGULATION
OF
PRB
ACTIVITY
8
REGULATION
OF
CDK4
AND
CDK6
COMPLEXES
9
REGULATION
OF
E2F
10
THE
PRB
PATHWAY
AND
CANCER
11
:ERIAL
AND
METHODS
13
2.1
2.2
2.3
2.4
2.5
2.6
2.7
2.8
2.9
2.10
2.11
2.12
2.13
2.14
TUMOR
BIOPSIES
13
CELL
CULTURE
13
CELL
STAINING
AND
MICROSCOPY
14
TRANSFECTIONS
14
IMMUNOLOGICAL
METHODS
15
KINASE
ASSAYS
15
ANTIBODIES
16
IN
VITRO
BINDING
EXPERIMENTS
16
CELL
CYCLE
ANALYSIS
17
PCR
AND
SEQUENCING
17
SOUTHERN
AND
NORTHERN
BLOT
ANALYSIS
18
EMSA
19
DETERMINATION
OF
HETEROZYGOSITY
IN
THE
RB
GENE
19
PCR-SSCP
20
22
3.
RESULTS
3.1
RATIONALE
22
3.2
THE
P53
PATHWAY
23
3.2.1
EXPERIMENTAL
STRATEGY
TO
TEST
FOR
A
FUNCTIONAL
P53
PATHWAY
23
3.2.2
P53
PROTEIN
LEVELS
IN
SCC
LINES
23
3.2.3
DNA
DAMAGE
RESPONSE
IN
SCC
LINES
23
3.3
THE
PRB
PATHWAY
26
3.3.1
ANALYSIS
OF
PRB
IN
ORAL
CARCINOMA
BIOPSIES
26
3.3.1.1
ANALYSIS
OF
LOSS
OF
HETEROZYGOSITY
(LOH)
26
3.3.1.2
ANALYSIS
OF
PRB
MUTATIONS
BY
SINGLE
STRAND
CONFORMATION
POLYMORPHISM
(SSCP)
27
3.3.2
EXPERIMENTAL
STRATEGY
TO
TEST
FOR
A
FUNCTIONAL
PRB
PATHWAY
29
3.3.3
STATUS
OF
THE
PRB
PROTEIN
IN
SCC
LINES
32
3.3.3.1
PHOSPHORYLATION
STATUS
OF
PRB
IN
CELL
LINES
32
3.3.3.2
ABILITY
OF
PRB
TO
BIND
TO
A
VIRAL
ONCOPROTEIN,
E7
32
3.3.4
CELL
CYCLE
ANALYSIS
32
3.3.5
STATUS
OF
THE
E2F-1
PROTEIN
34
3.3.5.1
E2F-1
PROTEIN
LEVELS
35
3.3.5.2
SEQUENCE
ANALYSIS
OF
THE
PRB
BINDING
DOMAIN
OF
E2F-1
35
3.3.5.3
ELECTROPHORETIC
MOBILITY
SHIFT
ANALYSIS
(EMSA)
OF
E2F-1
35
3.3.6
STATUS
OF
THE
PRB
KINASES
CDK4
AND
CDK6
39
3.3.6.1
STATUS
OF
CDK4
39
3.3.6.2
STATUS
OF
CDK6
41
3.3.7
D-TYPE
CYCLINS
44
3.3.7.1
CYCLIN
DI
44
33.7.2
CYCLIN
D2
AND
CYCLIN
D3
46
3.3.8
ASSOCIATION
OF
CDK4
AND
CDK6
WITH
LOW
MOLECULAR
WEIGHT
PROTEINS
47
3.3.9
STATUS
OF
CYCLIN-DEPENDENT
KINASE
INHIBITORS
50
3.3.9.1
P21DPI
50
3.3.9.2
PL8 NK4C
50
3.3.9.3
PL6INK4A
53
3.4
THE
PRB
PATHWAY
IS
PARTLY
RESTORED
BY
RE-EXPRESSION
OF
PL6INK*
A
58
3.4.1
EXPERIMENTAL
DESIGN
FOR
RE-EXPRESSING
ENDOGENOUS
PL6
INK4A
58
3.4.2
METHYLATION
STATUS
OF
PL6
INK4
A
58
3.4.2.1
METHYLATION
STATUS
IN
ORAL
CELL
LINES
58
3.4.2.2
METHYLATION
STATUS
AFTER
TREATMENT
WITH
A
DEMETHYLATING
AGENT,
5-AZA-2'-DEOXYCYTIDINE
60
3.4.3
PL6INK4A
EXPRESSION
AFTER
5-AZA-2'-DEOXYCYTIDINE
TREATMENT
63
3.4.4
EXPRESSION
OF
OTHER
CELL
CYCLE
REGULATORY
PROTEINS
IN
RESPONSE
TO
5-AZA-2'-DEOXYCYTIDINE
TREATMENT
63
3.4.5
CDK4
AND
CDK6
KINASE
ASSAYS
AFTER
5-AZA-2'-DEOXYCYTIDINE
TREATMENT
63
3.4.6
PHOSPHORYLATION
STATUS
OF
PRB
AFTER
5-AZA-2'-DEOXYCYTIDINE
TREATMENT
66
3.4.7
MORPHOLOGICAL
CHANGES
AFTER
5-AZA-2'-DEOXYCYTIDINE
TREATMENT
68
3.4.8
INDUCTION
OF
DIFFERENTIATION
MARKERS
AFTER
5-AZA-2'-DEOXYCYTIDINE
TREATMENT
68
4,
SUMMARY
AND
DISCUSSION
70
4.1
SUMMARY
70
4.2
THE
P53
PATHWAY
IS
DISRUPTED
BY
MUTATIONS
IN
THE
P53
PROTEIN
73
4.3
A
MODEL
FOR
DEREGULATION
OF
THE
PRB
PATHWAY
BY
SPECIFIC
ELEVATION
OF
CDK6
KINASE
ACTIVITY
74
4.4
RE-EXPRESSION
OF
PL6
INK4A
RESTORES
THE
PRB
PATHWAY
79
4.5
CONCLUDING
REMARKS
80
5.
REFERENCES
82
6.
DEUTSCHE
ZUSAMMENFASSUNG
101
ANMERKUNG
104
LEBENSLAUF
105
UNTERZEICHNETE
ERKLARUNG
106 |
| any_adam_object | 1 |
| author | Timmermann, Stefanie Alexandra 1967- |
| author_GND | (DE-588)12042052X |
| author_facet | Timmermann, Stefanie Alexandra 1967- |
| author_role | aut |
| author_sort | Timmermann, Stefanie Alexandra 1967- |
| author_variant | s a t sa sat |
| building | Verbundindex |
| bvnumber | BV012192035 |
| ctrlnum | (OCoLC)51616732 (DE-599)BVBBV012192035 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV012192035</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">980929s1997 gw ad|| m||| 00||| ger d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">954385209</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)51616732</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV012192035</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">ger</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-83</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Timmermann, Stefanie Alexandra</subfield><subfield code="d">1967-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)12042052X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis</subfield><subfield code="c">vorgelegt von Stefanie Alexandra Timmermann</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1997</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">106 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Köln, Univ., Diss., 1997</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Retinoblastom</subfield><subfield code="0">(DE-588)4177902-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Mundhöhlenkrebs</subfield><subfield code="0">(DE-588)4040733-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Carcinogenese</subfield><subfield code="0">(DE-588)4069853-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Mundhöhlenkrebs</subfield><subfield code="0">(DE-588)4040733-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Carcinogenese</subfield><subfield code="0">(DE-588)4069853-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Retinoblastom</subfield><subfield code="0">(DE-588)4177902-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008262338&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-008262338</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV012192035 |
| illustrated | Illustrated |
| indexdate | 2024-08-19T00:23:37Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008262338 |
| oclc_num | 51616732 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-83 DE-11 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-83 DE-11 DE-188 |
| physical | 106 S. Ill., graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| record_format | marc |
| spelling | Timmermann, Stefanie Alexandra 1967- Verfasser (DE-588)12042052X aut Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis vorgelegt von Stefanie Alexandra Timmermann 1997 106 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1997 Retinoblastom (DE-588)4177902-2 gnd rswk-swf Mundhöhlenkrebs (DE-588)4040733-0 gnd rswk-swf Carcinogenese (DE-588)4069853-1 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Mundhöhlenkrebs (DE-588)4040733-0 s Carcinogenese (DE-588)4069853-1 s Retinoblastom (DE-588)4177902-2 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008262338&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Timmermann, Stefanie Alexandra 1967- Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis Retinoblastom (DE-588)4177902-2 gnd Mundhöhlenkrebs (DE-588)4040733-0 gnd Carcinogenese (DE-588)4069853-1 gnd |
| subject_GND | (DE-588)4177902-2 (DE-588)4040733-0 (DE-588)4069853-1 (DE-588)4113937-9 |
| title | Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis |
| title_auth | Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis |
| title_exact_search | Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis |
| title_full | Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis vorgelegt von Stefanie Alexandra Timmermann |
| title_fullStr | Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis vorgelegt von Stefanie Alexandra Timmermann |
| title_full_unstemmed | Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis vorgelegt von Stefanie Alexandra Timmermann |
| title_short | Deregulation of the pRB pathway by a novel mechanism, and disruption of the p53 pathway during oral carcinogenesis |
| title_sort | deregulation of the prb pathway by a novel mechanism and disruption of the p53 pathway during oral carcinogenesis |
| topic | Retinoblastom (DE-588)4177902-2 gnd Mundhöhlenkrebs (DE-588)4040733-0 gnd Carcinogenese (DE-588)4069853-1 gnd |
| topic_facet | Retinoblastom Mundhöhlenkrebs Carcinogenese Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008262338&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT timmermannstefaniealexandra deregulationoftheprbpathwaybyanovelmechanismanddisruptionofthep53pathwayduringoralcarcinogenesis |