RNA isolation and characterization protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1998
|
| Schriftenreihe: | Methods in molecular biology
86 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 264 S. Ill., graph. Darst. |
| ISBN: | 0896034941 0896033937 |
Internformat
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| 245 | 1 | 0 | |a RNA isolation and characterization protocols |c ed. by Ralph Rapley ... |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1998 | |
| 300 | |a XIII, 264 S. |b Ill., graph. Darst. | ||
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| 650 | 7 | |a RNA |2 gtt | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
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Datensatz im Suchindex
| _version_ | 1804126725982912512 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
1 Introduction to Isolating RNA,
Donald E. Macfarlane and Christopher E. Dahle 1
2 Large and Small Scale RNA Preparations from Eukaryotic Cells,
Wolfgang Uckert, Wolfgang Walther, and Ulrike Stein 7
3 An Improved Rapid Method of Isolating RNA from Cultured Cells,
David B. Batt, Gordon G. Carmichael, and Zhong Liu 15
4 Isolating RNA with the Cationic Surfactant, Catrimox 14,
Christopher E. Dahle and Donald E. Macfarlane 19
5 RNA Extraction from Formalin Fixed and Paraffin Embedded
Tissues,
Giorgi Stanta, Serena Bonin, and Rosella Perin 23
6 Extraction and Purification of RNA from Plant Tissues Enriched
in Polysaccharides,
Shu Hua Cheng and Jeffrey R. Seemann 27
7 Isolation of Plant Mitochondrial RNA from Green Leaves,
Fei Ye, Wolfgang O. Abel, and Ralf Reski 33
8 Extraction of RNA from Fresh and Frozen Blood,
Bimal D. M. Theophilus 39
9 Isolation of Total RNA from Bacteria,
John Heptinstall 47
10 Isolation of Total RNA from Tissues or Cell Lines:
Visualization in Gel,
Tapas Mukhopadhyay and Jack A. Roth 55
11 Isolation of Messenger RNA,
Sian Bryant and David L. Manning 61
12 UV Spectrometric Analysis of Ribonucleic Acids
Ralph Rapley and John Heptinstall 65
vii
Vjjj Contents
13 Formaldehyde Gel Electrophoresis of Total RNA,
Sian Bryant and David L Manning 69
14 Preparation of RNA Dot Blots,
Rachel Hodge 73
15 Nonradioactive Northern Blotting,
RainerLow 77
16 The Use of RNA Probes for the Analysis of Gene Expression:
Northern Blot Hybridization and Ribonuclease Protection
Assay,
Dominique Belin 87
17 Analysis of RNA by Northern Blotting Using Riboprobes,
RaiAjit K. Srivastava 103
18 RNA Quantitative Analysis from Fixed and Paraffin Embedded
Tissues,
Giorgi Stanta, Serena Bonin, and Rene Utrera 113
19 Quantitative Analysis of RNA Species by PCR and Solid Phase
Minisequencing,
Anu Suomalainen and Ann Christine Syvanen 121
20 Preparation of Tissue Sections and Slides for mRNA
Hybridization,
Giorgi Terenghi 133
21 Detecting mRNA in Tissue Sections with Digoxigenin Labeled
Probes,
Giorgi Terenghi 137
22 One Tube RT PCR with Sequence Specific Primes,
Ulrich Pfeffer U3
23 Identification of Differentially Exposed Genes by Nonradioactive
Differential Display Messenger RNA,
Thomas C. G. Bosch and Jan U. Lohmann 153
24 Characterization of RNA Using Continuous RT PCR Coupled
with ELOSA,
Francois Mallet, Guy Oriol, and Bernard Mandrand 161
25 Gene Expression Analysis by CD RT PCR,
Eric de Kant 173
26 Primer Extension Analysis of mRNA,
Maggie Walmsley, Mark Leonard, and Roger Patient 187
Contents ix
27 S1 Mapping Using Single Stranded DNA Probes,
Stephane Viville and Roberto Mantovani 195
28 Measurements of Rate of Transcription in Isolated Nuclie
by Nuclear Run Off Assay,
Rai Ajit K. Srivastava and Gustov Schonfeld 201
29 Transcription In Vitro Using Bacteriophage RNA Polymerases,
Elaine T. Schenhborn 209
30 In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte
Lysate Cell Free System,
Louise Olliver and Charles D. Boyd 221
31 In Vitro Translation of Messenger RNA in a Wheat Germ
Extract Cell Free System,
Louise Olliver, Anne Grobler Rabie, Charles D. Boyd 229
32 The Xenopus Egg Extract Translation System,
Glenn M. Matthews and Alan Colman 235
33 Purification and Characterization of Viral dsRNA Genome
Profiles by Crosshybridization,
Lesley Ann Martin and Peter P. C. Mertens 249
Index 261
|
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| indexdate | 2024-07-09T18:22:02Z |
| institution | BVB |
| isbn | 0896034941 0896033937 |
| language | English |
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| spelling | RNA isolation and characterization protocols ed. by Ralph Rapley ... Totowa, NJ Humana Press 1998 XIII, 264 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 86 Isolering (proces) gtt Karakterisering (algemeen) gtt RNA gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten RNA Analysis Laboratory manuals RNA Purification Laboratory manuals RNA isolation & purification Laboratory Manuals RNS (DE-588)4076759-0 gnd rswk-swf Isolierung Chemie (DE-588)4122223-4 gnd rswk-swf Chemische Analyse (DE-588)4009840-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS (DE-588)4076759-0 s Isolierung Chemie (DE-588)4122223-4 s DE-604 Chemische Analyse (DE-588)4009840-0 s Rapley, Ralph Sonstige oth Methods in molecular biology 86 (DE-604)BV035362695 86 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008207668&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | RNA isolation and characterization protocols Methods in molecular biology Isolering (proces) gtt Karakterisering (algemeen) gtt RNA gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten RNA Analysis Laboratory manuals RNA Purification Laboratory manuals RNA isolation & purification Laboratory Manuals RNS (DE-588)4076759-0 gnd Isolierung Chemie (DE-588)4122223-4 gnd Chemische Analyse (DE-588)4009840-0 gnd |
| subject_GND | (DE-588)4076759-0 (DE-588)4122223-4 (DE-588)4009840-0 (DE-588)4143413-4 |
| title | RNA isolation and characterization protocols |
| title_auth | RNA isolation and characterization protocols |
| title_exact_search | RNA isolation and characterization protocols |
| title_full | RNA isolation and characterization protocols ed. by Ralph Rapley ... |
| title_fullStr | RNA isolation and characterization protocols ed. by Ralph Rapley ... |
| title_full_unstemmed | RNA isolation and characterization protocols ed. by Ralph Rapley ... |
| title_short | RNA isolation and characterization protocols |
| title_sort | rna isolation and characterization protocols |
| topic | Isolering (proces) gtt Karakterisering (algemeen) gtt RNA gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten RNA Analysis Laboratory manuals RNA Purification Laboratory manuals RNA isolation & purification Laboratory Manuals RNS (DE-588)4076759-0 gnd Isolierung Chemie (DE-588)4122223-4 gnd Chemische Analyse (DE-588)4009840-0 gnd |
| topic_facet | Isolering (proces) Karakterisering (algemeen) RNA Wetenschappelijke technieken Wissenschaftliches Arbeiten RNA Analysis Laboratory manuals RNA Purification Laboratory manuals RNA isolation & purification Laboratory Manuals RNS Isolierung Chemie Chemische Analyse Aufsatzsammlung |
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