Phospholipid signaling protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1998
|
| Schriftenreihe: | Methods in molecular biology
105 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 380 S. Ill., graph. Darst. |
| ISBN: | 0896034917 |
Internformat
MARC
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| 245 | 1 | 0 | |a Phospholipid signaling protocols |c ed. by Ian M. Bird. |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1998 | |
| 300 | |a XII, 380 S. |b Ill., graph. Darst. | ||
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| 650 | 4 | |a Membrane lipids |x Research |x Methodology | |
| 650 | 4 | |a Phospholipids |x Research |x Methodology | |
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Datensatz im Suchindex
| _version_ | 1804126716207038464 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I. Monitoring of Activation of Phospholipid Derived Cell Signaling
Pathways
1 Phosphoinositidase C Activation Assay I: Cell Labeling, Stimulation,
and Recovery of Cellular fHJPhosphoinositides
and ^HJPhosphoinositols
Ian M. Bird 7
2 Phosphoinositidase C Activation Assay II: Simple Analysis
of Recovered Cellular Phosphoinositides and Phosphoinositols
lanM. Bird 11
3 Phosphoinositidase C Activation Assay III: HPLC Analysis of Cellular
Phosphoinositides and Phosphoinositols
lanM. Bird 25
4 Measurement of Phosphoinositols and Phosphoinositides Using
Radio High Performance Liquid Chromatography Flow Detection
Lubo Zhang and lain L. O. Buxton 47
5 Preparation of [3H]Phosphoinositol Standards and Conversion
of [3H]Phosphoinositides to [3H]Phosphoinositols
Ian M. Bird 65
6 Inositol 1,4,5 Trisphosphate Mass Assay
Lubo Zhang 77
7 Measurement of Cellular Diacylglycerol Content
Wendy Bollinger Bollag and Richard D. Griner 89
8 Phosphatidylinositol 4 Kinases: Assays and Product Analysis
Rachel E. Meyers and Lewis C. Cantley 99
9 Phosphatidylinositol(3.4.5)Trisphosphate (Ptdins(3.4.5)P_) Mass
Measurement Using a Radioligand Displacement Assay
Jeroen van der Kaay, Pete J. Cullen, and C. Peter Downes 109
vii
10 Detection of Phosphatidylinositol 4 Phosphate 5 Kinase Activity
Using Thin Layer Chromatography
Gregory J. Parker, Joost C. Loijens, and Richard A. Anderson... 127
11 Determination of Phospholipase C or Phospholipase D Catalyzed
Phosphatidylcholine Hydrolysis
Michael J. O. Wakelam and Trevor R. Pettitt 141
12 Measurement of Phospholipase D Activity
Wendy Bollinger Bollag 151
13 Monitoring of Phospholipase A2 Activation in Cultured Cells Using
Tritiated Arachidonic Acid
Sandie I. Briand, Sylvie G. Bernier, and Gaetan Guillemette 161
14 Assay of Cellular Diacylglycerol and Monoacylglycerol Upases
Bryan A. Wolf 167
15 Isotopic Efflux Studies as Indices of Phospholipase Activation
Jacob Vadakekalam and Stewart Metz 175
16 Extraction and Measurements of Prostanoids and Leukotrienes
by Radioimmunoassays
Bruno Battistini, Serge Picard, Pierre Borgeat,
and Pierre Sirois 187
17 Measurement of Prostanoids, Leukotrienes, and Isoprostanes
by Enzyme Immunoassays
Bruno Battistini, Serge Picard, Pierre Borgeat,
and Pierre Sirois 201
18 Measurements of Arachidonic Acid Metabolites Derived
from the Lipoxygenase Pathways by High Pressure Liquid
Chromatography
Pierre Borgeat, Serge Picard, Bruno Battistini,
and Pierre Sirois 209
19 Measurement of Sphingomyelin and Ceramide Cellular Levels After
Sphingomyelinase Mediated Sphingomyelin Hydrolysis
Pino Santana, Luisa F. Fanjul, and C. M. Ruiz de Galarreta 217
20 Sphingomyelin and Ceramide Mass Assay
Pino Santana, C. M. Ruiz de Galarreta, and Luisa F. Fanjul 223
21 Sphingosine Kinase: Assay and Product Analysis
Ana Olivera and Sarah Spiegel 233
22 Analytical Methods and Steps to Sample Preparation
for Determination of Molecular Species of Fatty Acids
Sanda Clejan 243
23 HPLC Analytical Methods for the Separation of Molecular Species
of Fatty Acids In Diacylglycerol and Cellular Phospholipids
Sanda Clejan 255
24 Analysis of Molecular Species of Cellular Sphingomyelins
and Ceramides
Sanda Clejan 275
Part II. General Methods for Monitoring Changes in Levels of Key Signaling
Pathway Proteins and Associated mRNA
25 Solubilization and Assay of Cellular and Tissue Protein
Cynthia E. Shaw 287
26 Western Immunoblot Analysis
Cynthia E. Shaw and Jing Zheng 295
27 Immunohistochemistry and Immunocytochemistry
Jing Zheng 307
28 Extraction of Cellular and Tissue RNA
DanielS. Millican and Ian M. Bird 315
29 Size Separation and Quantification of mRNA by Northern Analysis
Ian M. Bird 325
30 Preparation of Single Stranded Antisense cDNA Probes
by Asymmetric PCR
DanielS. Millican and Ian M. Bird 337
31 Optimization of a Reverse Transcription Polymerase Chain Reaction
(RT PCR) Mass Assay for Low Abundance mRNA
Jacqueline M. Cale, Cynthia E. Shaw, and Ian M. Bird 351
Index 373
|
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| id | DE-604.BV012111806 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:21:52Z |
| institution | BVB |
| isbn | 0896034917 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008200807 |
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| physical | XII, 380 S. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Phospholipid signaling protocols ed. by Ian M. Bird. Totowa, NJ Humana Press 1998 XII, 380 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 105 Cellular signal transduction Research Methodology Membrane lipids Research Methodology Phospholipids Research Methodology Second messengers (Biochemistry) Research Methodology Methode (DE-588)4038971-6 gnd rswk-swf Phospholipidmembran (DE-588)4174401-9 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Phospholipide (DE-588)4131432-3 gnd rswk-swf Zellkommunikation (DE-588)4136131-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Phospholipide (DE-588)4131432-3 s Signaltransduktion (DE-588)4318717-1 s DE-604 Phospholipidmembran (DE-588)4174401-9 s Zellkommunikation (DE-588)4136131-3 s Methode (DE-588)4038971-6 s Bird, Ian M. Sonstige oth Methods in molecular biology 105 (DE-604)BV035362695 105 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008200807&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Phospholipid signaling protocols Methods in molecular biology Cellular signal transduction Research Methodology Membrane lipids Research Methodology Phospholipids Research Methodology Second messengers (Biochemistry) Research Methodology Methode (DE-588)4038971-6 gnd Phospholipidmembran (DE-588)4174401-9 gnd Signaltransduktion (DE-588)4318717-1 gnd Phospholipide (DE-588)4131432-3 gnd Zellkommunikation (DE-588)4136131-3 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4174401-9 (DE-588)4318717-1 (DE-588)4131432-3 (DE-588)4136131-3 (DE-588)4143413-4 |
| title | Phospholipid signaling protocols |
| title_auth | Phospholipid signaling protocols |
| title_exact_search | Phospholipid signaling protocols |
| title_full | Phospholipid signaling protocols ed. by Ian M. Bird. |
| title_fullStr | Phospholipid signaling protocols ed. by Ian M. Bird. |
| title_full_unstemmed | Phospholipid signaling protocols ed. by Ian M. Bird. |
| title_short | Phospholipid signaling protocols |
| title_sort | phospholipid signaling protocols |
| topic | Cellular signal transduction Research Methodology Membrane lipids Research Methodology Phospholipids Research Methodology Second messengers (Biochemistry) Research Methodology Methode (DE-588)4038971-6 gnd Phospholipidmembran (DE-588)4174401-9 gnd Signaltransduktion (DE-588)4318717-1 gnd Phospholipide (DE-588)4131432-3 gnd Zellkommunikation (DE-588)4136131-3 gnd |
| topic_facet | Cellular signal transduction Research Methodology Membrane lipids Research Methodology Phospholipids Research Methodology Second messengers (Biochemistry) Research Methodology Methode Phospholipidmembran Signaltransduktion Phospholipide Zellkommunikation Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008200807&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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