Immunochemical protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1998
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
80 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 508 S. Ill., graph. Darst. |
| ISBN: | 0896034933 0896033880 |
Internformat
MARC
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| 245 | 1 | 0 | |a Immunochemical protocols |c ed. by John D. Pound |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1998 | |
| 300 | |a XIII, 508 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 80 | |
| 650 | 7 | |a Immunochemie |2 gtt | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Antibodies |x isolation & purification |v Laboratory Manuals | |
| 650 | 4 | |a Immunochemistry |x Methodology | |
| 650 | 4 | |a Immunochemistry |x methods |v Laboratory Manuals | |
| 650 | 0 | 7 | |a Molekularbiologie |0 (DE-588)4039983-7 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1804126677611053056 |
|---|---|
| adam_text | Contents
Preface v
List of Contributors xi
1 Production of Polyclonal Antisera
Jonathan A. Green and Margaret M. Manson /
2 Raising Polyclonal Antibodies Using Nitrocellulose Bound Antigen
Monique Diano, Andre Le Bivic, and Michel Him 5
3 Production and Characterization of Antibodies Against Synthetic
Peptides
David C. Hancock and Gerard I. Evan 15
4 Preparation and Testing of Monoclonal Antibodies
to Recombinant Proteins
Christopher J. Dean 23
5 A Simple Hollow Fiber Bioreactor for the In House Production
of Monoclonal Antibodies
Margaret Goodaii 39
6 Screening of Monoclonal Antibodies Using Antigens Labeled
with Acetylcholinesterase
Yveline Frobert and Jacques Grassi 57
7 Synthesis of Peptides for Use as Immunogens
David C. Hancock, Nicola J. O Reilly, and Gerard I. Evan 69
8 Use of Proteins Blotted to Polyvinylidene Difluoride Membranes
as Immunogens
Boguslaw Szewczyk and Donald F. Summers 81
9 Purification of Glycoproteins and Their Use as Immunogens
Boguslaw Szewczyk and Donald F. Summers 87
10 IgG Purification
Mark Page and Robin Thorpe 95
11 Purification of Monoclonal Antibodies
Mark Page and Robin Thorpe 113
12 Production of Bispecific and Trispecific F(ab)2 and F(ab)3 Antibody
Derivatives
Ruth R. French 121
vii
viii Contents
13 Preparation of Cytotoxic Antibody—Toxin Conjugates
AlanJ. Cumber and Edward J. Wawrzynczak 135
14 Immunoaffinity Purification and Quantification of Antibody—Toxin
Conjugates
Edward J. Wawrzynczak and Alan J. Cumber 145
15 Competitive ELISA
Kittima Makarananda, Lucinda R. Weir, and Gordon E. Neal 155
16 Epitope Mapping
Glenn E. Morris 161
17 Coupling of Antibodies with Biotin
Rosaria P. Haugland and Wendy W. You 173
18 Preparation of Avidin Conjugates
Rosaria P. Haugland and Mahesh K. Bhalgat 185
19 Enhanced Chemiluminescence Immunoassay
Richard A. W. Stott 197
20 Nonradioactive Methods for Visualization of Protein Blots
Michael Finney 207
21 Colloidal Gold Staining and Immunoprobing on the Same
Western Blot
Denise Egger and Kurt Bienz 217
22 Erasure of Western Blots After Autoradiographic
or Chemiluminescent Detection
Scott H. Kaufmann and Udo Kellner 223
23 Colloidal Gold Staining and Immunodetection in 2D Protein Mapping
Anthony H. V. Schapira 237
24 Enzyme Antienzyme Method for Immunohistochemistry
Michael G. Ormerod, Elizabeth Philp, and Susanne F. Imrie 243
25 Immunohistochemical Detection of Bromodeoxyuridine Labeled
Nuclei for In Vivo Cell Kinetic Studies
Jonathan A. Green, Richard E. Edwards,
and Margaret M. Manson 257
26 Cell Kinetic Studies Using a Monoclonal Antibody
to Bromodeoxyuridine
George D. Wilson 255
27 Immunohistochemical Detection of Cells in the Division Cycle Using
Antibodies to Proliferating Cell Nuclear Antigen (PCNA)
Richard E. Edwards and Jerry Styles 267
Contents ix
28 Double Label Immunohistochemistry on Tissue Sections Using
Alkaline Phosphatase and Peroxidase Conjugates
Jonathan A. Green and Margaret M. Manson 271
29 Preparation of Colloidal Gold Probes
David A. Hughes and Julian E. Beesley 275
30 Immunogold Probes in Light Microscopy
David A. Hughes and Julian E. Beesley 283
31 Immunogold Probes in Electron Microscopy
David J. P. Ferguson, David A. Hughes,
and Julian E. Beesley 297
32 Electron Microscopic Silver Enhancement for Double Labeling
with Antibodies Raised in the Same Species
Kurt Bienz and Denise Egger 313
33 Flow Cytometric Analysis of Cell Surface Antigen Density
R. Adrian Robins 319
34 Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry
R. Adrian Robins 337
35 Flow Cytometric Methods of Analyzing Apoptotic Cells
Anne E. Milner, J. Michaela Levens,
and Christopher D. Gregory 347
36 Flow Cytometric Detection of Proliferation Associated Antigens,
PCNA and Ki 67
George D. Wilson 355
37 Sorting of Human Peripheral Blood T Cell Subsets Using
Immunomagnetic Beads
Eddie C. Y. Wang 365
38 Preparation and Use of Nonradioactive Hybridization Probes
Victor T. W. Chan 377
39 Cellular Human and Viral DNA Detection by Nonisotopic In Situ
Hybridization
C. S. Herrington 385
40 Biotinylated Probes in Colony Hybridization
Michael J. Haas 397
41 Nonisotopic In Situ Hybridization: Gene Mapping and Cytogenetics
Bhupendra Bhatt, Tulin Sahinoglu, and Cliff Stevens 405
42 Expression of Recombinant Antibody Fusion Proteins in E. coli
Stephen M. Hobbs 419
x Contents
43 Expression of Antibody Fusion Proteins in Mammalian Cells
Anita A. Hamilton, John R. Adair, and Simon J. Forster 427
44 Antibody Screening of Bacteriophage X gt 11 DNA Expression Libraries
Peter Jones 439
45 Display of Antibody Chains on Filamentous Bacteriophage
Peter Jones 449
46 Production of Human Fab Antibody Fragments from Phage Display
Libraries
J. Mark Hexham 461
47 Antibodies from Phage Display Libraries
as Immunochemical Reagents
Dario Neri, Alessandro Pini, and Ahuva Nissim 475
Index 501
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV012076383 |
| callnumber-first | Q - Science |
| callnumber-label | QR183 |
| callnumber-raw | QR183.6 |
| callnumber-search | QR183.6 |
| callnumber-sort | QR 3183.6 |
| callnumber-subject | QR - Microbiology |
| classification_rvk | WC 4150 WD 4150 |
| ctrlnum | (OCoLC)37588535 (DE-599)BVBBV012076383 |
| dewey-full | 616.07/56 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.07/56 |
| dewey-search | 616.07/56 |
| dewey-sort | 3616.07 256 |
| dewey-tens | 610 - Medicine and health |
| discipline | Biologie Medizin |
| edition | 2. ed. |
| format | Book |
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| id | DE-604.BV012076383 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:21:15Z |
| institution | BVB |
| isbn | 0896034933 0896033880 |
| language | English |
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| physical | XIII, 508 S. Ill., graph. Darst. |
| publishDate | 1998 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Immunochemical protocols ed. by John D. Pound 2. ed. Totowa, NJ Humana Press 1998 XIII, 508 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 80 Immunochemie gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Antibodies isolation & purification Laboratory Manuals Immunochemistry Methodology Immunochemistry methods Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Immunchemie (DE-588)4161366-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Immunchemie (DE-588)4161366-1 s Methode (DE-588)4038971-6 s DE-604 Molekularbiologie (DE-588)4039983-7 s Pound, John D. Sonstige oth Methods in molecular biology 80 (DE-604)BV035362695 80 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008175640&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Immunochemical protocols Methods in molecular biology Immunochemie gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Antibodies isolation & purification Laboratory Manuals Immunochemistry Methodology Immunochemistry methods Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Immunchemie (DE-588)4161366-1 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4038971-6 (DE-588)4161366-1 (DE-588)4143413-4 |
| title | Immunochemical protocols |
| title_auth | Immunochemical protocols |
| title_exact_search | Immunochemical protocols |
| title_full | Immunochemical protocols ed. by John D. Pound |
| title_fullStr | Immunochemical protocols ed. by John D. Pound |
| title_full_unstemmed | Immunochemical protocols ed. by John D. Pound |
| title_short | Immunochemical protocols |
| title_sort | immunochemical protocols |
| topic | Immunochemie gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Antibodies isolation & purification Laboratory Manuals Immunochemistry Methodology Immunochemistry methods Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Immunchemie (DE-588)4161366-1 gnd |
| topic_facet | Immunochemie Wetenschappelijke technieken Wissenschaftliches Arbeiten Antibodies isolation & purification Laboratory Manuals Immunochemistry Methodology Immunochemistry methods Laboratory Manuals Molekularbiologie Methode Immunchemie Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008175640&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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