Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
1997
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Frankfurt (Main), Univ., Diss., 1997 |
| Beschreibung: | V, 162 S. graph. Darst. |
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| 264 | 1 | |c 1997 | |
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Datensatz im Suchindex
| _version_ | 1807594089572466688 |
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| adam_text |
CONTENTS
I
CONTENTS
1.
INTRODUCTION
AND
AIM
OF
THE
THESIS
1
1.1.
THE
INTRACELLULAR
PH
1
1.2.
THE
REGULATION
OF
INTRACELLULAR
PH
3
1.2.1.
PROTON
TRANSPORTING
ATPASES
5
1.2.2.
LACTATE/H*
COTRANSPORTER
6
1.2.3.
NA
+
-INDEPENDENT
C17HC0/
EXCHANGER
(CBE)
7
1.2.4.
NA7HCOA"
COTRANSPORTER
(NBC)
8
1.2.5.
THENATBT
EXCHANGER
(NHE)
10
1.2.5.1.
BASIC
PROPERTIES
OF
THE
NA
+
/H*
EXCHANGER
10
1.2.5.1.1.
STOICHIOMETRY
10
1.2.5.1.2.
SELECTIVITY
11
1.2.5.1.3.
KINETICS
11
1.2.5.1.4.
PRIMARY
AND
SECONDARY
STRUCTURE
12
1.2.5.1.5.
INHIBITION
14
I.2.5.2.
PHYSIOLOGICAL
ROLE
OF
THE
NA7FT
EXCHANGER
16
1.2.5.2.1.
REGULATION
OF
INTRACELLULAR
PH
16
I.2.5.2.2.
CONTROL
OF
CELL
VOLUME
17
I.2.5.2.3.
TRANSEPITHELIAL
TRANSPORT
17
I.2.5.2.4.
CONTROL
OF
CELL
GROWTH
AND
PROLIFERATION
18
1.2.5.3.
PATHOPHYSIOLOGICAL
ROLE
OF
THE
NA7H*
EXCHANGER
20
1.2.5.3.1.
MYOCARDIAL
ISCHAEMIA
AND
REPERFUSION
20
I.2.5.3.2.
ESSENTIAL
HYPERTENSION
24
I.2.5.3.3.
CANCER
26
I.2.5.3.4.
DIABETIC
NEPHROPATHY
27
N
CONTENTS
I.2.5.4.
NA
+
/LT
EXCHANGER
SUBTYPES
29
1.2.5.4.
1.NHE1
29
I.2.5.4.2.
NHE2
30
I.2.5.4.3.
NHE3
31
I.2.5.4.4.
NHE4
32
I.2.5.4.5.
NHE5
33
1.2.6.
THE
NA
+
-DEPENDENT
CITHCOJ
'
EXCHANGER
(NCBE)
34
1.2.6.1.
BASIC
PROPERTIES
OF
THE
NA*-DEPENDENT
CITHCOJ
'
EXCHANGER
34
1.2.6.1.1.
STOICHIOMETRY
34
1.2.6.1.2.
SELECTIVITY
AND
KINETICS
35
1.2.6.1.3,
INHIBITION
35
1.2.6.2.
PHYSIOLOGICAL
ROLE
OF
THE
NA*-DEPENDENT
CITHCOJ
'
EXCHANGER
37
1.2.6.2.1.
REGULATION
OF
INTRACELLULAR
PH
37
I.2.6.2.2.
CONTROL
OF
CELL
GROWTH
AND
PROLIFERATION
38
1.2.6.3.
PATHOPHYSIOLOGICAL
ROLE
OF
THE
NA*-DEPENDENT
CITHCOA'
39
EXCHANGER
1.3.
MEASUREMENT
OF
INTRACELLULAR
PH
40
1.3.1.
DISTRIBUTION
OF
WEAK
ACIDS
AND
BASES
40
1.3.2.
PH-SENSITIVE
MICROELECTRODES
40
1.3.3.
NUCLEAR
MAGNETIC
RESONANCE
SPECTROSCOPY
(NMR)
41
1.3.4.
PH-SENSITIVE
FLUORESCENT
DYES
41
1.4.
AIM
OF
THE
THESIS
45
CONTENTS
M
2.
MATERIALS
AND
METHODS
47
2.1.
CELL
CULTURE
47
2.1.1.
BOVINE
AORTIC
ENDOTHELIAL
CELLS
47
2.1.2.
MOUSE
LUNG
FIBROBLAST
CELL
LINE
LAP1
48
2.1.3.
TRANSFECTED
FIBROBLAST
CELL
LINES
48
2.2.
ASSAYS
49
2.2.1.
MEASUREMENT
OF
PH
49
2.2.2.
MEASUREMENT
OF
PH;
RECOVERY
FROM
INTRACELLULAR
ACIDIFICATION
IN
50
BOVINE
AORTIC
ENDOTHELIAL
CELLS
2.2.3.
SCREENING
ASSAY
OF
THE
NA7H*
EXCHANGER
IN
BOVINE
AORTIC
ENDOTHELIAL
51
CELLS
2.2.4.
SCREENING
ASSAY
OF
THE
NA'TH
'
EXCHANGER
SUBTYPES
51
2.2.5.
SCREENING
ASSAY
OF
THE
NA*-DEPENDENT
CLTHCOJ'
EXCHANGER
52
23.
SOLUTIONS
AND
SUBSTANCES
53
2.3.1.
SOLUTIONS
53
2.3.2.
SUBSTANCES
53
2.4.
DATA
ANALYSIS
54
2.4.1.
DETERMINATION
OF
ICSO
VALUES
54
2.4.2.
STATISTICS
55
IV
CONTENTS
3.
RESULTS
56
3.1.
INTRACELLULAR
PH
REGULATION
IN
BOVINE
AORTIC
ENDOTHELIAL
CELLS
56
3.1.1.
DETERMINATION
OF
PH,
56
3.1.2.
PHJ
RECOVERY
AFTER
AN
ACID
LOAD
WITH
THE
NHC1
PREPULSE
TECHNIQUE
58
3.1.3.
PHI
RECOVERY
AFTER
AN
ACID
LOAD
WITH
CO2/HCO3'
64
3.2.
NA
+
/LT
EXCHANGE
IN
BOVINE
AORTIC
ENDOTHELIAL
CELLS
AND
ITS
INHIBITION
68
3.2.1.
NA7H*
EXCHANGE
68
3.2.2.
INHIBITION
OFNA7H*
EXCHANGE
70
3.2.3.
COMPARISON
OF
INHIBITORY
NHE
POTENCIES
OF
GUANIDINE
DERIVATIVES
IN
79
BOVINE
AORTIC
ENDOTHELIAL
CELLS
AND
IN
RABBIT
ERYTHROCYTES
3.3.
NA
+
/EF
EXCHANGER SUBTYPES
(NHE1,
NHE2,
AND
NHE3)
80
3.3.1.
EXCHANGE
ACTIVITIES
OF
NHE
SUBTYPES
AFTER
INTRACELLULAR
ACIDIFICATION
80
3.3.2.
SUBTYPE-SPECIFICITY
OFPOTENTIALNHE
INHIBITORS
83
3.4.
NA
'
-DEPENDENT
CI
/HCOJ
'
EXCHANGE
AND
ITS
INHIBITION
85
3.4.1.
DEMONSTRATION
OF
DEFECTIVE
NA7H*
EXCHANGERACTIVITY
IN
LAP
1
CELLS
85
3.4.2.
DEMONSTRATION
OF
NA^-DEPENDENT
CLTHCCH'
EXCHANGER
IN
LAP1
CELLS
87
3.4.3.
INHIBITION
OF
THE
NA
+
-DEPENDENT
CLTHCQJ'
EXCHANGER
90
CONTENTS
V
4.
DISCUSSION
98
4.1.
METHOD
DEVELOPMENT
98
4.2.
INTRACELLULAR
PH
REGULATION
IN
BOVINE
AORTIC
ENDOTHELIAL
CELLS
102
4.3.
NAVET
EXCHANGE
IN
BOVINE
AORTIC
ENDOTHELIAL
CELLS
AND
ITS
INHIBITION
106
4.4.
NA
+
/H*
EXCHANGER
SUBTYPES
(NHE1,
NHE2,
AND
NHE3)
111
4.5.
NA*-DEPENDENT
CLTHCOJ"
EXCHANGE
AND
ITS
INHIBITION
115
5.
SUMMARY
119
6.
ZUSAMMENFASSUNG
125
7.
GLOSSARY
OF
ABBREVIATIONS
133
8.
REFERENCES
135
9.
ACTIVE
PARTICIPATIONS
IN
CONGRESSES
AND
SYMPOSIA
160
10.
PUBLICATIONS
161
11.
CURRICULUM
VITAE
162 |
| any_adam_object | 1 |
| author | Faber, Sabine 1955- |
| author_GND | (DE-588)120821532 |
| author_facet | Faber, Sabine 1955- |
| author_role | aut |
| author_sort | Faber, Sabine 1955- |
| author_variant | s f sf |
| building | Verbundindex |
| bvnumber | BV012052125 |
| ctrlnum | (OCoLC)64632315 (DE-599)BVBBV012052125 |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV012052125 |
| illustrated | Illustrated |
| indexdate | 2024-08-17T00:54:15Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008157459 |
| oclc_num | 64632315 |
| open_access_boolean | |
| owner | DE-29T DE-355 DE-BY-UBR |
| owner_facet | DE-29T DE-355 DE-BY-UBR |
| physical | V, 162 S. graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| record_format | marc |
| spelling | Faber, Sabine 1955- Verfasser (DE-588)120821532 aut Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition vorgelegt von Sabine Faber 1997 V, 162 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Frankfurt (Main), Univ., Diss., 1997 Natriumion (DE-588)4171261-4 gnd rswk-swf Natrium-Wasserstoff-Antiport (DE-588)4443085-1 gnd rswk-swf Regulation (DE-588)4049075-0 gnd rswk-swf Wasserstoffion (DE-588)4189272-0 gnd rswk-swf Chloridion (DE-588)4147794-7 gnd rswk-swf Intrazellulärraum (DE-588)4330337-7 gnd rswk-swf Wasserstoffionenkonzentration (DE-588)4189273-2 gnd rswk-swf Hydrogencarbonate (DE-588)4280442-5 gnd rswk-swf Cotransport (DE-588)4246072-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Intrazellulärraum (DE-588)4330337-7 s Wasserstoffionenkonzentration (DE-588)4189273-2 s Regulation (DE-588)4049075-0 s Natrium-Wasserstoff-Antiport (DE-588)4443085-1 s DE-604 Chloridion (DE-588)4147794-7 s Hydrogencarbonate (DE-588)4280442-5 s Cotransport (DE-588)4246072-4 s Natriumion (DE-588)4171261-4 s Wasserstoffion (DE-588)4189272-0 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008157459&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Faber, Sabine 1955- Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition Natriumion (DE-588)4171261-4 gnd Natrium-Wasserstoff-Antiport (DE-588)4443085-1 gnd Regulation (DE-588)4049075-0 gnd Wasserstoffion (DE-588)4189272-0 gnd Chloridion (DE-588)4147794-7 gnd Intrazellulärraum (DE-588)4330337-7 gnd Wasserstoffionenkonzentration (DE-588)4189273-2 gnd Hydrogencarbonate (DE-588)4280442-5 gnd Cotransport (DE-588)4246072-4 gnd |
| subject_GND | (DE-588)4171261-4 (DE-588)4443085-1 (DE-588)4049075-0 (DE-588)4189272-0 (DE-588)4147794-7 (DE-588)4330337-7 (DE-588)4189273-2 (DE-588)4280442-5 (DE-588)4246072-4 (DE-588)4113937-9 |
| title | Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition |
| title_auth | Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition |
| title_exact_search | Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition |
| title_full | Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition vorgelegt von Sabine Faber |
| title_fullStr | Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition vorgelegt von Sabine Faber |
| title_full_unstemmed | Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition vorgelegt von Sabine Faber |
| title_short | Assays for examination of intracellular pH regulation via Na1hn+/H1hn+ exchanger and Na1hn+-dependent Cl1hn-/HCO1tn31hn- exchanger and their inhibition |
| title_sort | assays for examination of intracellular ph regulation via na1hn h1hn exchanger and na1hn dependent cl1hn hco1tn31hn exchanger and their inhibition |
| topic | Natriumion (DE-588)4171261-4 gnd Natrium-Wasserstoff-Antiport (DE-588)4443085-1 gnd Regulation (DE-588)4049075-0 gnd Wasserstoffion (DE-588)4189272-0 gnd Chloridion (DE-588)4147794-7 gnd Intrazellulärraum (DE-588)4330337-7 gnd Wasserstoffionenkonzentration (DE-588)4189273-2 gnd Hydrogencarbonate (DE-588)4280442-5 gnd Cotransport (DE-588)4246072-4 gnd |
| topic_facet | Natriumion Natrium-Wasserstoff-Antiport Regulation Wasserstoffion Chloridion Intrazellulärraum Wasserstoffionenkonzentration Hydrogencarbonate Cotransport Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008157459&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT fabersabine assaysforexaminationofintracellularphregulationviana1hnh1hnexchangerandna1hndependentcl1hnhco1tn31hnexchangerandtheirinhibition |