HPLC columns: theory, technology, and practice
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Wiley-VCH
1997
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 393 S. graph. Darst. |
| ISBN: | 0471190373 |
Internformat
MARC
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Datensatz im Suchindex
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| adam_text | HPLC COLUMNS THEORY, TECHNOLOGY, AND PRACTICE UWE D. NEUE WITH A
CONTRIBUTION FROM M. ZOUBAIR EL FALLAH WILEY-VCH NEW YORK * CHICHESTER *
WEINHEIM * BRISBANE * SINGAPORE * TORONTO CONTENTS PREFACE IX 1
INTRODUCTION 1 1.1 HISTORY 1 1.2 SCOPE OF THIS BOOK 3 1.3 WHAT IS HPLC?
3 1.4 SEPARATION MECHANISMS USED IN HPLC 4 2 THEORY OF CHROMATOGRAPHY 6
2.1 DEFINITIONS 7 2.1.1 ISOCRATIC AND GRADIENT CHROMATOGRAPHY 7 2.1.2
THE HEIGHT EQUIVALENT TO A THEORETICAL PLATE 7 2.1.3 RESOLUTION 9 2.1.4
EFNCIENCY, PLATE COUNT 11 2.1.5 PEAK ASYMMETRY 13 2.1.6 LINEAR VELOCITY
14 2.1.7 REDUCED PARAMETERS 15 2.2 HYDRODYNAMICS OF CHROMATOGRAPHY 16
2.2.1 VAN DEEMTER EQUATION 17 2.2.2 GIDDINGS EQUATION 23 2.2.3 KNOX
EQUATION 27 2.2.4 COMPARISON OF THE DIFFERENT EQUATIONS 28 2.2.5
PERMEABILITY 29 2.2.6 ESTIMATION OF THE DIFFUSION COEFFICIENT IN THE
MOBILE PHASE 33 2.2.7 PERFORMANCE INDEX AND SEPARATION IMPEDANCE 33 2.3
THERMODYNAMICS OF CHROMATOGRAPHY 35 2.3.1 DEFINITION OF THE RETENTION
FACTOR 35 2.3.2 RETENTION FACTOR AND CHEMICAL EQUILIBRIUM 36 2.3.3
RELATIVE RETENTION A 38 2.3.4 RELATIONSHIPS TO THE FREE ENERGY 38
REFERENCES 39 ADDITIONAL READING 40 VIII CONTENTS COLUMN DESIGN 41 3.1
CHOICE OF COLUMN DIMENSIONS AND PARTICLE SIZE 41 3.1.1 INFLUENCE OF
PARTICLE SIZE AND COLUMN LENGTH 42 3.1.2 INFLUENCE OF COLUMN DIAMETER 49
3.2 DESIGN LIMITS 53 3.2.1 HEAT OF FRICTION 54 3.2.2 EXTRACOLUMN EFFECTS
55 3.3 COLUMN HARDWARE 61 3.3.1 END-FITTING DESIGN 61 3.3.2 TUBING 64
3.3.3 COLUMNS OR CARTRIDGES? 65 3.4 ALTERNATIVE COLUMN DESIGNS 66 3.4.1
RADIAL COMPRESSION 67 3.4.2 AXIAL COMPRESSION 70 3.4.3 ALTERNATIVES TO
PACKED BEDS 70 3.4.3.1 MEMBRANES 71 3.4.3.2 MONOLITHIC STRUCTURES 72
3.4.3.3 FIBERS 72 3.4.4 EVALUATING ALTERNATIVE DESIGNS 73 REFERENCES 79
4 PHYSICAL PROPERTIES OF HPLC PACKINGS 81 4.1 PARTICLE SIZE, PARTICLE
SHAPE, AND PARTICLE SIZE DISTRIBUTION 81 4.2 POROSITY, PORE SIZE, AND
SPECIFIC SURFACE AREA 86 4.3 POROUS VERSUS NONPOROUS PARTICLES 89 4.4
PARTICLE STRENGTH 90 REFERENCES 91 5 COLUMN PACKING AND TESTING 93 5.1
COLUMN PACKING 94 5.1.1 PRINCIPLES 94 5.1.2 APPARATUS 96 5.1.3 SLURRY
PREPARATION 97 5.1.4 PACKING TECHNIQUES 99 5.2 COLUMN TESTING 101 5.3
SUMMARY 103 REFERENCES 104 ADDITIONAL READING 104 OTHER LITERATURE
RELATED TO COLUMN PACKING 104 CONTENTS IX 6 COLUMN CHEMISTRY 106 6.1
INORGANIC VERSUS POLYMERIE PACKINGS 107 6.2 SILICA-BASED BONDED PHASES
109 6.2.1 BONDING TECHNOLOGY 110 6.2.2 MONOMERIC VERSUS POLYMERIE
BONDING 111 6.2.3 TYPES OF BONDED PHASES 115 6.2.4 REPRODUCIBILITY OF
BONDED PHASES 119 6.2.5 ALTERNATIVE BONDING TECHNOLOGIES 120 6.3 THE
CHEMISTRY OF POLYMERIE PACKINGS 122 6.3.1 DIVINYLBENZENE-BASED PACKINGS
122 6.3.2 METHACRYLATE-BASED PACKINGS 124 6.3.3 VINYLALCOHOL-BASED
PACKINGS 125 6.4 OTHER SURFACE MODIFICATION TECHNIQUES FOR INORGANIC
PACKINGS 125 6.4.1 PROPERTIES OF OTHER INORGANIC PACKINGS 126 6.4.2
OTHER SURFACE MODIFICATION TECHNIQUES 127 REFERENCES 128 ADDITIONAL
READING 129 COLUMN SELECTION 7.1 INTRODUCTION 7.2 POLYMER ANALYSIS 7.3
PROTEIN ANALYSIS 7.4 PEPTIDE ANALYSIS 7.5 NUCLEIC ACID ANALYSIS 7.6
CARBOHYDRATE ANALYSIS 7.7 ANALYSIS OF INORGANIC IONS 7.8 ANALYSIS OF
LOW-MOLECULAR-WEIGHT ORGANIC COMPOUNDS 7.9 SUMMARY 130 130 131 132 134
134 135 136 136 139 8 SIZE-EXCLUSION CHROMATOGRAPHY 140 8.1 SEPARATION
MECHANISM 140 8.2 SELECTION OF MOBILE PHASE AND COLUMN TYPE 146 8.3
SELECTION OF THE PORE SIZE 151 8.4 SELECTION OF THE PARTICLE SIZE 154
8.5 POLYMERIE PACKINGS FOR ORGANIC SEC 157 8.6 POLYMERIE PACKINGS FOR
AQUEOUS SEC 158 8.7 SILICA-BASED PACKINGS 159 8.8 PRACTICAL ASPECTS OF
SEC 161 REFERENCES 162 X CONTENTS 9 NORMAL-PHASE CHROMATOGRAPHY 164 9.1
RETENTION MECHANISM 164 9.2 SILICA 171 9.3 SILICA-BASED BONDED PHASES
175 9.4 ALUMINA 178 9.5 SPECIAL TECHNIQUES 179 9.6 PRACTICAL ASPECTS OF
NORMAL-PHASE CHROMATOGRAPHY 180 REFERENCES 181 ADDITIONAL READING 182 10
REVERSED-PHASE CHROMATOGRAPHY 183 10.1 RETENTION MECHANISM 184 10.2
SILICA-BASED BONDED PHASES 191 10.2.1 SILANOL-GROUP ACTIVITY 194 10.2.2
CHARACTERIZATION OF PACKINGS 197 10.2.3 REPRODUCIBUEITY OF REVERSED-PHASE
PACKINGS 201 10.3 POLYMERIE REVERSED-PHASE PACKINGS 204 10.3.1
STYRENE-DIVINYLBENZENE-BASED PACKINGS 204 10.3.2 METHACRYLATE-BASED
PACKINGS 205 10.3.3 POLYVINYLALCOHOL-BASED PACKINGS 205 10.4 OTHER TYPES
206 10.4.1 POROUS GRAPHITIZED CARBON 206 10.4.2 POLYMER-COATED ALUMINA
207 10.4.3 INTERNAL-SURFACE REVERSED-PHASE PACKINGS 207 10.4.4
IMMOBILIZED ARTIFICIAL MEMBRANES 208 10.5 SPECIAL TECHNIQUES 209 10.5.1
PAIRED-ION CHROMATOGRAPHY 209 10.5.2 RPLC OF PEPTIDES 211 10.6 PRACTICAL
ASPECTS OF REVERSED-PHASE CHROMATOGRAPHY 213 10.6.1 COLUMN SELECTION 213
10.6.2 MOBILE-PHASE CONSIDERATIONS 214 10.6.3 COLUMN CARE 214 REFERENCES
215 ADDITIONAL READING 216 11 HYDROPHILIC INTERACTION CHROMATOGRAPHY 217
11.1 RETENTION MECHANISM 217 11.2 PACKINGS FOR HILIC 218 11.3
APPLICATIONS 220 11.4 PRACTICAL ASPECTS 221 REFERENCES 223 CONTENTS XI
12 ION-EXCHANGE CHROMATOGRAPHY 224 12.1 RETENTION MECHANISM 224 12.1.1
GENERAL ION-EXCHANGE MECHANISM 225 12.1.2 BEHAVIOR OF COIONS 228 12.1.3
ELUTION STRENGTH SCALES 229 12.1.4 INFLUENCE OF PH 230 12.1.5
ION-EXCHANGE CAPACITY 232 12.1.6 NONIDEAL INTERACTIONS 233 12.2
ION-EXCHANGE PACKINGS 234 12.2.1 SILICA-BASED ION EXCHANGERS 234 12.2.2
ION EXCHANGERS BASED ON ORGANIC POLYMERS 235 12.2.3 ION EXCHANGERS FOR
ION CHROMATOGRAPHY 238 12.3 ION CHROMATOGRAPHY 239 12.4 ION-EXCHANGE
SEPARATIONS OF BIOPOLYMERS 241 12.4.1 PROTEINS 242 12.4.2 NUCLEIC ACIDS
245 12.5 PRACTICAL ASPECTS 247 REFERENCES 249 ADDITIONAL READING 249 13
HYDROPHOBIE INTERACTION CHROMATOGRAPHY 250 251 253 254 256 258 259 260
261 261 261 263 265 267 268 273 275 15 PREPARATIVE CHROMATOGRAPHY 276
15.1 PRINCIPLES 276 13.1 13.2 13.3 13.4 13.5 RETENTION MECHANISM
OPERATING CONDITIONS PACKINGS FOR HIC APPLICATIONS PRACTICAL ASPECTS
REFERENCES ADDITIONAL READING 14 SPECIAL TECHNIQUES 14.1 14.2 14.3 14.4
14.5 COLUMN COUPLING 14.1.1 PRINCIPLES OF COLUMN COUPLING 14.1.2 GUARD
COLUMNS COLUMN SWITCHING RECYCLING AND WALKING COLUMN SIMULATED MOVING
BED CONTINUOUS CHROMATOGRAPHY REFERENCES XII CONTENTS 15.1.1 INFLUENCE
OF THE INJECTION VOLUME 277 15.1.2 INFLUENCE OF MASS OVERLOAD 278 15.1.3
INFLUENCE OF COLUMN DISPERSION 284 15.1.4 COMBINED EFFECT OF INJECTION
VOLUME, MASS OVERLOAD, AND DISPERSION 284 15.2 TECHNOLOGIES FOR
LABORATORY-SCALE PREPARATIVE CHROMATOGRAPHY 288 15.3 PRACTICAL ASPECTS
290 15.3.1 LOADING STUDIES 290 15.3.2 SCALING LAWS 291 15.3.3 PARTICLE
SIZE 294 REFERENCES 295 ADDITIONAL READING 295 16 HPLC METHODS
DEVELOPMENT 296 16.1 INTRODUCTION 296 16.2 THEORETICAL CONSIDERATIONS
297 16.2.1 LINEAR-SOLVENT-STRENGTH THEORY 297 16.2.2 CHARACTERISTIC
PARAMETERS IN LINEAR-SOLVENT-STRENGTH GRADIENTS 298 16.2.2.1 RETENTION
TIME 298 16.2.2.2 PEAK WIDTH 299 16.2.2.3 RESOLUTION 300 16.2.3
RELATIONSHIP BETWEEN SOLVENT STRENGTH AND MOBILE-PHASE COMPOSITION 301
16.2.4 MEASURING THE QUALITY OF SEPARATION BETWEEN ADJACENT PEAKS 302
16.2.4.1 RESOLUTION 302 16.2.4.2 OTHER SEPARATION CRITERIA 303 16.2.4.3
THE DISCRIMINATION FACTOR 303 16.2.5 BASIS OF MOBILE-PHASE SELECTIVITY
IN HPLC 308 16.3 METHOD DEVELOPMENT STRATEGY 310 16.3.1 CONSIDERATIONS
IN THE CHOICE OF ISOCRATIC VERSUS GRADIENT ELUTION 310 16.3.2 DEVELOPING
ISOCRATIC SEPARATIONS 315 16.3.2.1 BINARY MOBILE PHASES 319 16.3.2.2
SOLVENT SELECTIVITY AND TERNARY MOBILE PHASES 323 16.3.2.3 SEPARATION OF
TOTALLY OR PARTIALLY UNKNOWN MIXTURES 327 16.3.3 DEVELOPING GRADIENT
SEPARATIONS 329 16.3.4 COMPUTER-ASSISTED METHOD DEVELOPMENT 332 CONTENTS
XUEI 16.4 ENHANCING SENSITIVITY 333 16.5 TEMPERATURE EFFECTS 337
REFERENCES 339 17 COLUMN MAINTENANCE 342 17.1 CARE AND USE OF
CHROMATOGRAPHIE COLUMNS 343 17.1.1 INSTALLATION AND EQUILIBRATION 343
17.1.2 COLUMN USE 346 17.1.3 PERFORMANCE MONITORING DURING USE 347
17.1.4 COLUMN PROTECTION 349 17.1.5 STORAGE 350 17.2 TROUBLESHOOTING OF
COLUMN PROBLEMS 351 17.2.1 PEAK-SHAPE PROBLEMS 351 17.2.1.1 EXTRA-COLUMN
EFFECTS 352 17.2.1.1.1 EXTRA-COLUMN BAND SPREADING 352 17.2.1.1.2
DETECTOR TIME CONSTANT 354 17.2.1.2 COLUMN OVERLOAD 355 17.2.1.2.1
VOLUME OVERLOAD 355 17.2.1.2.2 MASS OVERLOAD 356 17.2.1.3 DETECTOR
OVERLOAD 356 17.2.1.4 MOBILE PHASE OVERLOAD 356 17.2.1.4.1 PH-CONTROL
356 17.2.1.4.2 INCORRECT SAMPLE SOLVENT 357 17.2.1.4.3 SOLUBILITY 357
17.2.1.5 TAILING DUE TO SECONDARY INTERACTIONS 359 17.2.1.6 SAMPLE
DISTRIBUTION 359 17.2.1.7 COLUMN COLLAPSE 360 17.2.1.8 SECONDARY
EQUILIBRIA 362 17.2.2 RETENTION-TIME PROBLEMS 365 17.2.2.1 RETENTION
TIME REPRODUCIBILITY 365 17.2.2.1.1 TEMPERATURE 365 17.2.2.1.2
MOBILE-PHASE COMPOSITION 366 17.2.2.2 RETENTION TIME DRIFT 369
17.2.2.2.1 COLUMN EQUILIBRATION 367 17.2.2.2.2 STATIONARY-PHASE
STABILITY 369 17.2.2.2.3 COLUMN CONTAMINATION 370 17.2.2.2.4
MOBILE-PHASE COMPOSITION 372 17.2.2.2.5 OTHER COLUMN AGING MECHANISMS
372 17.2.2.2.6 HYDROPHOBIE COLLAPSE 373 17.2.2.3 COLUMN-TO-COLUMN
REPRODUCIBILITY 374 XIV CONTENTS 17.2.3 MISCELLANEOUS PROBLEMS 375
17.2.3.1 EXTRA PEAKS 375 17.2.3.2 EXCESSIVE BACKPRESSURE 378 17.2.3.3
BASELINE DRIFT 379 REFERENCES 379 APPENDIX: TEACHING COLUMN
CHROMATOGRAPHY 380 LIST OF SYMBOLS 383 INDEX 389
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| id | DE-604.BV012049815 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:20:47Z |
| institution | BVB |
| isbn | 0471190373 |
| language | English |
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| physical | XVI, 393 S. graph. Darst. |
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| spelling | Neue, Uwe D. Verfasser aut HPLC columns theory, technology, and practice Uwe D. Neue New York [u.a.] Wiley-VCH 1997 XVI, 393 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier HPLC (DE-588)4072641-1 gnd rswk-swf HPLC (DE-588)4072641-1 s DE-604 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008156091&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Neue, Uwe D. HPLC columns theory, technology, and practice HPLC (DE-588)4072641-1 gnd |
| subject_GND | (DE-588)4072641-1 |
| title | HPLC columns theory, technology, and practice |
| title_auth | HPLC columns theory, technology, and practice |
| title_exact_search | HPLC columns theory, technology, and practice |
| title_full | HPLC columns theory, technology, and practice Uwe D. Neue |
| title_fullStr | HPLC columns theory, technology, and practice Uwe D. Neue |
| title_full_unstemmed | HPLC columns theory, technology, and practice Uwe D. Neue |
| title_short | HPLC columns |
| title_sort | hplc columns theory technology and practice |
| title_sub | theory, technology, and practice |
| topic | HPLC (DE-588)4072641-1 gnd |
| topic_facet | HPLC |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008156091&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT neueuwed hplccolumnstheorytechnologyandpractice |