Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
1997
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Stuttgart, Univ., Diss., 1997 |
| Beschreibung: | XXII, 142 S. Ill., graph. Darst. |
Internformat
MARC
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| 008 | 980331s1997 gw ad|| m||| 00||| ger d | ||
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| 041 | 0 | |a ger | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 | ||
| 100 | 1 | |a Soumanou, Mohamed M. |d 1959- |e Verfasser |0 (DE-588)115846417 |4 aut | |
| 245 | 1 | 0 | |a Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position |c von Mohamed M. Soumanou |
| 264 | 1 | |c 1997 | |
| 300 | |a XXII, 142 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Stuttgart, Univ., Diss., 1997 | ||
| 650 | 0 | 7 | |a Triglyceride |0 (DE-588)4127027-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Lipasen |0 (DE-588)4167788-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Enzymkatalyse |0 (DE-588)4152480-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Umesterung |0 (DE-588)4222675-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Enzymkatalyse |0 (DE-588)4152480-9 |D s |
| 689 | 0 | 1 | |a Lipasen |0 (DE-588)4167788-2 |D s |
| 689 | 0 | 2 | |a Umesterung |0 (DE-588)4222675-2 |D s |
| 689 | 0 | 3 | |a Triglyceride |0 (DE-588)4127027-7 |D s |
| 689 | 0 | |5 DE-604 | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008024775 | |
Datensatz im Suchindex
| _version_ | 1807229772756942848 |
|---|---|
| adam_text |
XVI
C
ONTENT
ACKNOWLEDGMENTS
N
ABSTRACT
V
ZUSAMMENFASSUNG
VII
CONTENT
XVI
ABBREVIATIONS
XXI
1
I
NTRODUCTION
1
1.1
AIM
OF
THE
WORK
1
1.2
PRODUCTION
AND
IMPORTANCE
OF
FATS
AND
OILS
3
1.3
PHYSICAL
PROPERTIES
OF
FATS
AND
OILS
4
1.4
NUTRITIONAL
FUNCTIONS
OF
FATS
AND
OILS
5
1.5
FAT
PROCESSING
8
1.5.1
HYDROGENATION
8
1.5.2
INTERESTERIFICATION
9
1.5.3
FRACTIONATION
11
1.6
LIPASES
11
1.6.1
LIPASE
SOURCES
12
1.6.1.1
MICROBIAL
LIPASES
12
1.6.1.2
MAMMALIAN
LIPASES
12
1.6.1.3
PLANT
LIPASES
14
1.6.2
MECHANISM
OF
ACTION
OF
LIPASES
14
1.6.2.1
INTERFACIAL
ACTIVATION
OF
LIPASES
14
1.6.2.2
THE
THREE
DIMENSIONAL
STRUCTURES
OF
LIPASES
16
1.6.3
SUBSTRATE
SPECIFICITY
19
1.6.4
LIPASES
IN
NON-AQUEOUS
MEDIA
20
1.6.4.1
CHOICE
OF
SOLVENT
20
1.6.4.2
WATER
CONTENT
AND
WATER
ACTIVITY
21
XVN
1.6.4.3
DETERMINATION
OF
WATER
ACTIVITY
22
1.6.4.4
EFFECT
OF
WATER
ACTIVITY
ON
ENZYME
ACTIVITY
23
1.6.5
MODIFIED
LIPASE
24
1.6.5.1
ADSORPTIVE
BINDING
24
1.6.5.2
COVALENT
BINDING
25
1.6.5.3
ENTRAPMENT
AND
LIPASE-SURFACTANT
COMPLEX
26
1.6.5.4
LIPASES
IN
MICROEMULSIONS
26
1.6.5.5
OTHER
NON-CONVENTIONAL
MEDIAS
27
1.6.6
LIPASE-CATALYZED
SYNTHESIS
OF
GLYCERIDES
27
1.6.6.1
LIPASE-CATALYZED
SYNTHESIS
OF
MONOACYLGLYCEROLS
27
1.6.6.2
LIPASE-CATALYZED
SYNTHESIS
OF
STRUCTURED
TRIGLYCERIDES
29
2
R
ESULTS
33
2.1
CHARACTERIZATION
OF
COMMERCIAL
LIPASES
33
2.2
SCREENING
OF
COMMERCIAL
LIPASES
FOR
HYDROLYSIS
33
2.3
ONE-STEP
REACTION
FOR
SYNTHESIS
38
2.3.1
ACIDOLYSIS
REACTION
38
2.3.1.1
EFFECT
OF
WATER
ACTIVITY
ON
REACTION
RATE
39
2.3.1.2
EFFECT
OF
SUPPORT
AND
IMMOBILIZATION
METHODS
42
2.3.1.3
EFFECT
OF
ORGANIC
SOLVENT
45
2.3.1.4
EFFECT
OF
TYPE
OF
ACYL
ACCEPTOR
AND
DONOR
48
2.3.1.5
EFFECT
OF
TEMPERATURE
52
2.3.2
TRANSESTERIFICATION
OF
TRIGLYCERIDES
MIXTURE
53
2.3.2.1
TIME
COURSE
OF
THE
TRANSESTERIFICATION
REACTION
54
2.3.2.2
EFFECT
OF
MOLAR
RATIO
OF
SUBSTRATES
56
2.3.2.3
EFFECT
OF
PH
58
XVIII
23.2.4
FREE
FATTY
ACID
COMPOSITION
59
23.2.5
INFLUENCE
OF
TEMPERATURE
ON
THE
STABILITY
OF
LIPASE
62
23.2.6
EFFECT
OF
REACTION
SYSTEM
64
2.3.3
TWO-STEP
ENZYMATIC
REACTION
65
23.3.1
ALCOHOLYSIS
REACTION
66
23.3.1.1
EFFECT
OF
THE
CHAIN-LENGTH
OF
THE
ALCOHOL
69
2.33.1.2
EFFECT
OF
THE
TYPE
OF
TRIGLYCERIDE
69
23.3.2
ESTERIFICATION
REACTION
BETWEEN
PURE
2-MG
72
WITH
MEDIUM-CHAIN
FATTY
ACIDS
23.3.2.1
EFFECT
OF
MOLAR
RATIO
OF
CAPRYLIC
ACID
TO
2-MO
73
2.33.2.2
EFFECT
OF
ORIGIN
OF
LIPASE
AND
SUPPORT
FOR
IMMOBILIZATION
74
2.3.3.23
EFFECT
OF
FATTY
ACID
CHAIN
LENGTH
ON
ESTERIFICATION
RATE
80
2.3.33
ESTERIFICATION
OF
MIXED
2-MG
WITH
MEDIUM-CHAIN
FATTY
ACID
80
23.3.3.1
EFFECT
OF
ORGANIC
SOLVENT
ON
ESTERIFICATION
REACTION
86
23.3.3.2
FATTY
ACID
DISTRIBUTION
OF
SYNTHESIZED
TRIGLYCERIDES
87
3
D
ISCUSSION
89
3.1
LIPASE
SCREENING
FOR
HYDROLYSIS
AND
SYNTHESIS
89
33
COMPARISON
OF
LIPASE
PERFORMANCES
IN
ACIDOLYSIS
AND
91
TRANSESTERIFICATION
REACTIONS
3.2.1
EFFECT
OF
ORGANIC
SOLVENT
91
3.2.2
SOLVENT
FREE
SYSTEM
92
3.2.3
EFFECT
OF
LIPASE
IMMOBILIZATION
92
3.3
EFFECT
OF
PH
94
3.4
STABILITY
OF
IMMOBILIZED
LIPASES
94
3.5
EFFECT
OF
TYPE
OF
SUBSTRATE
ON
INTERESTERIFICATION
RATE
95
XIX
3.6
CONCENTRATION
OF
THE
SYNTHESIZED
TRIGLYCERIDES
96
3.7
COMPARISON
OF
LIPASE
PERFORMANCES
IN
A
TWO-STEP
PROCESS
97
3.7.1
ALCOHOLYSIS
OF
PURE
TRIGLYCERIDES
AND
NATURAL
OILS
97
3.7.2
ESTERIFICATION
OF
2-MG
AND
MEDIUM-CHAIN
FATTY
ACIDS
98
4
C
ONCLUSION
105
5
M
ATERIAL
AND
M
ETHODS
107
5.1
MATERIAL
107
5.1.1
LIPASES
107
5.1.2
EQUIPMENTS
107
5.1.3
CHEMICALS
109
5.2
METHODS
111
5.2.1
LIPASE
CHARACTERIZATION
111
5.2.1.1
PROTEIN
DETERMINATION
111
5.2.1.2
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
111
5.2.1.3
ACTIVITY
STAINING
(ZYMOGRAM)
113
5.2.1.4
PROTEASE
ACTIVITY
114
5.2.1.5
HYDROLYTIC
ACTIVITY
114
5.2.2
IMMOBILIZATION
OF
LIPASE
114
5.2.2.1
ADSORPTIVE
BINDING
115
5.2.2.1.1
IMMOBILIZATION
OF
LIPASE
ON
CELITE
OR
HYFLO
SUPER
CEL
115
5.2.2.1.2
IMMOBILIZATION
OF
LIPASE
ON
POLYPROPYLENE
115
5.2.2.2
COVALENT
BINDING
115
5.2.3
WATER
CONTENT
AND
WATER
ACTIVITY
DETERMINATION
116
5.2.3.1
DETERMINATION
OF
WATER
CONTENT
116
XX
7
5.23.2
DETERMINATION
OF
WATER
ACTIVITY
117
5.2.4
INTERESTERIFICATION
REACTION
118
5.2.4.1
INTERESTERIFICATION
REACTION
BETWEEN
A
TRIGLYCERIDE
AND
FATTY
ACIDS
118
5.2.4.2
INTERESTERIFICATION
BETWEEN
TRIGLYCERIDES
118
5.2.5
TWO-STEP
ENZYMATIC
REACTION
119
5.2.5.1
ALCOHOLYSIS
OF
TRIGLYCERIDES
119
5.2.5.2
ESTERIFICATION
REACTION
119
5.2.6
PRODUCT
CHARACTERIZATION
120
5.2.6.1
PH
OF
THE
REACTION
MEDIUM
120
5.2.6.2
DETERMINATION
OF
FREE
FATTY
ACID
120
5.2.63
DETERMINATION
OF
FREE
FATTY
ACID
COMPOSITION
120
5.2.6.4
DETERMINATION
OF
BOUND
FATTY
ACID
120
5.2.6.5
DETERMINATION
OF
THE
FATTY
ACID
DISTRIBUTION
OF
TRIGLYCERIDES
121
5.2.6.6
GLYCERIDE
COMPOSITION
OF
THE
REACTION
MEDIUM
BY
TLC/FID
122
5.2.6.7
PURIFICATION
OF
PRODUCED
TG
123
5.2.6.8
HPLC
SEPARATION
OF
TRIACYLGLYCEROLS
123
5.2.6.9
NMR-SPECTROSCOPY
125
6
REFERENCES
126
CURRICULUM
VITAE
142 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV011876947 |
| illustrated | Illustrated |
| indexdate | 2024-08-13T00:23:36Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008024775 |
| oclc_num | 51997134 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | XXII, 142 S. Ill., graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| record_format | marc |
| spelling | Soumanou, Mohamed M. 1959- Verfasser (DE-588)115846417 aut Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position von Mohamed M. Soumanou 1997 XXII, 142 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Stuttgart, Univ., Diss., 1997 Triglyceride (DE-588)4127027-7 gnd rswk-swf Lipasen (DE-588)4167788-2 gnd rswk-swf Enzymkatalyse (DE-588)4152480-9 gnd rswk-swf Umesterung (DE-588)4222675-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Enzymkatalyse (DE-588)4152480-9 s Lipasen (DE-588)4167788-2 s Umesterung (DE-588)4222675-2 s Triglyceride (DE-588)4127027-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008024775&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Soumanou, Mohamed M. 1959- Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position Triglyceride (DE-588)4127027-7 gnd Lipasen (DE-588)4167788-2 gnd Enzymkatalyse (DE-588)4152480-9 gnd Umesterung (DE-588)4222675-2 gnd |
| subject_GND | (DE-588)4127027-7 (DE-588)4167788-2 (DE-588)4152480-9 (DE-588)4222675-2 (DE-588)4113937-9 |
| title | Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position |
| title_auth | Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position |
| title_exact_search | Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position |
| title_full | Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position von Mohamed M. Soumanou |
| title_fullStr | Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position von Mohamed M. Soumanou |
| title_full_unstemmed | Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position von Mohamed M. Soumanou |
| title_short | Lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position |
| title_sort | lipase catalyzed synthesis of structured triglycerides containing medium chain fatty acids in sn1 and sn3 position and a long chain fatty acid in sn2 position |
| topic | Triglyceride (DE-588)4127027-7 gnd Lipasen (DE-588)4167788-2 gnd Enzymkatalyse (DE-588)4152480-9 gnd Umesterung (DE-588)4222675-2 gnd |
| topic_facet | Triglyceride Lipasen Enzymkatalyse Umesterung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008024775&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT soumanoumohamedm lipasecatalyzedsynthesisofstructuredtriglyceridescontainingmediumchainfattyacidsinsn1andsn3positionandalongchainfattyacidinsn2position |