Molecular genetics of bacteria:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Chichester [u.a.]
Wiley
1998
|
| Ausgabe: | 3. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 310 S. Ill., graph. Darst. |
| ISBN: | 0471977837 0471977829 |
Internformat
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| 100 | 1 | |a Dale, Jeremy W. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Molecular genetics of bacteria |c Jeremy W. Dale |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a Chichester [u.a.] |b Wiley |c 1998 | |
| 300 | |a XIV, 310 S. |b Ill., graph. Darst. | ||
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| 650 | 7 | |a Bacteriën |2 gtt | |
| 650 | 7 | |a Genetica |2 gtt | |
| 650 | 4 | |a Genetic Engineering | |
| 650 | 4 | |a Genetic engineering | |
| 650 | 4 | |a Genetics, Microbial | |
| 650 | 4 | |a Microbial genetics | |
| 650 | 4 | |a Molecular Biology | |
| 650 | 4 | |a Molecular genetics | |
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Datensatz im Suchindex
| _version_ | 1804126339127574529 |
|---|---|
| adam_text | Contents
Preface xiii
Chapter 1 NUCLEIC ACID STRUCTURE AND FUNCTION 1
Structure of nucleic acids 1
DNA 1
RNA 4
Hydrophobic interactions 4
Different forms of the double helix 5
Supercoiling 7
Action of topoisomerases 10
Denaturation and hybridization 12
Orientation of nucleic acid strands 14
Replication of DNA 14
Unwinding and rewinding 16
Fidelity of replication; proof reading 17
DNA repair 17
Excision repair 18
Recombination (post replication) repair 20
SOS repair 20
Gene expression 22
Transcription 22
Transcriptional terminators 24
Translation 26
The genetic code 26
Ribosomes 28
Transfer RNA 28
Mechanism of protein synthesis 31
Post translational events 33
vi Molecular Genetics of Bacteria
Secretion 34
Other post translational modifications 34
Gene organization 34
Chapter 2 MUTATION AND VARIATION 39
Variation and evolution 39
Fluctuation test 40
Directed mutation in bacteria? 42
Types of mutations 43
Point mutations 43
Variation due to larger scale DNA alterations 45
Extrachromosomal agents 46
Phenotypes 46
Reversion and suppression 49
Conditional mutants 51
Complementation 51
Recombination 52
Mechanisms of mutation 53
Spontaneous mutation 53
Chemical mutagens 55
Ultraviolet irradiation 58
Photoreactivation 59
SOS repair 61
Isolation and identification of mutants 62
Mutation and selection 62
Replica plating 63
Penicillin enrichment 65
Isolation of other mutants 65
Reverse genetics 66
Chapter 3 REGULATION OF GENE EXPRESSION 69
Gene copy number 71
Transcriptional control 71
Promoters 71
Alternative promoters 74
Terminators, attenuators and antiterminators 76
Induction and repression: the lac operon 77
Regulatory mutants of the lac operon 79
Catabolite repression 82
Other operons 84
Arabinose operon 84
Biosynthesis: trp operon 85
Contents vii
Upstream activator sequences and enhancers 89
Two component regulatory systems 90
Global control systems 92
Translational control 93
Ribosome binding 93
Codon usage 95
Stringent response 96
Antisense RNA 96
Developmental regulation: quorum sensing and
cross talking 97
Chapter 4 GENETICS OF BACTERIOPHAGES 99
Single strand DNA bacteriophages 103
j |3X174 103
M13 106
RNA containing phages: MS2 106
Double stranded DNA phages 107
Bacteriophage T4 107
Replication and packaging 107
Control of phage development 109
Bacteriophage X 110
Replication and packaging 110
Lysogeny 112
Lyric and lysogenic regulation of bacteriophage K 116
Temporal control of the lytic cycle 116
Control of lysogeny 118
Mechanism of binding of repressor and
Cro proteins to the operator site 120
Superinfection immunity and zygotic
induction 122
Restriction and modification 123
Complementation and recombination 126
Chapter 5 EXTRACHROMOSOMAL INHERITANCE 129
Some bacterial characteristics are determined by
plasmids 129
Antibiotic resistance 129
Colicins and bacteriocins 130
Virulence determinants 131
Plasmids in plant associated bacteria 132
Metabolic activities 133
Biodegradation and bioremediation 133
viii Molecular Genetics of Bacteria
Molecular properties of plasmids 134
Plasmid maintenance and control of replication 136
Replication of ColEl 138
Replication of R100 139
Control of plasmid replication by DNA
repeats (iterons) 142
Plasmid replication via single stranded forms 143
Replication of linear plasmids 147
Plasmid stability 148
Plasmid integrity 150
Partitioning 150
Differential growth rate 152
Classification of plasmids 154
Antibiotic resistance pattern 154
Incompatibility groups 154
Phage susceptibility 155
Molecular characterization 155
Host range 155
Methods for studying plasmids 157
Tests of plasmid involvement 157
Detection and isolation of plasmid DNA 159
Extrachromosomal inheritance in eukaryotes 161
Chapter 6 GENE TRANSFER 163
Transformation 164
Natural competence 164
Induced competence 165
Protoplast transformation 167
Electroporation 168
Conjugation 169
Mechanism of conjugation 169
Mating pair formation 169
Transfer of DNA 170
Mobilization and chromosomal transfer 172
The F plasmid 172
Hfr strains 173
Integration and excision of F; formation of F
plasmids 173
Conjugation in other bacteria 175
Conjugative transposons 175
Transduction 176
Specialized transduction 178
Contents ix
Recombination 179
General (homologous) recombination 179
Site specific and non homologous (illegitimate)
recombination 184
Mosaic genes and chromosome plasticity 185
Chapter 7 MOVABLE GENES 187
Insertion sequences 187
Structure of insertion sequences 188
Occurrence of insertion sequences 190
Transposons 191
Structure of transposons 193
Integrons 195
Mechanisms of transposition 196
Replicative transposition 196
Molecular basis of replicative transposition 196
Non replicative (conservative) tranposition 198
Regulation of transposition 202
Activation of genes by transposable elements 203
Mu: a transposable bacteriophage 203
Inversions 204
Salmonella phase variation 204
Inversion in phage Mu 206
Antigenic variation 207
Variation in the gonococcus 208
Borrelia 208
Trypanosoma brucei 209
Mating type genes in Saccharomyces cerevisiae 210
Chapter 8 GENETIC MODIFICATION 211
Strain development 211
Generation of variation 211
Selection of desired variants 212
Genetic exchange 213
Overproduction of primary metabolites 213
Simple pathways 214
Feedback regulation 214
Antimetabolites 215
Branched pathways 215
Lysine production 217
Glutamic acid production 218
Overproduction of secondary metabolites 218
x Molecular Genetics of Bacteria
Penicillin production 219
Rational screening 220
Production of novel metabolites 221
Mutational biosynthesis 221
Gene cloning 221
Cutting and joining DNA 222
Plasmid vectors 223
Origin of replication 223
Selectable marker 223
Cloning site 225
Insertional inactivation 225
Bacteriophage X vectors 225
Insertion vectors 225
Replacement vectors 227
Cloning larger fragments 227
Bacteriophage M13 vectors 228
Gene libraries 229
Construction of genomic libraries 229
Screening a gene library 229
Screening with gene probes 231
Polymerase chain reaction 231
Screening gene libraries with antibodies 233
Construction of a cDNA library 233
Expression vectors 235
Xgtll 236
Maximizing product formation 237
Making new genes 237
DNA synthesis 237
Site directed mutagenesis 237
Protein engineering 239
Products from recombinant E. coli 239
Other host vector systems 240
Bacterial hosts 240
Shuttle vectors 241
Eukaryotic hosts 242
Cloning in animal and plant cells; transgenic
plants and animals 243
Novel vaccines 243
Chapter 9 GENETIC ANALYSIS 245
Microbial biochemistry and physiology 245
Metabolic pathways 245
Contents xi
Complementation 246
Cross feeding 247
Arginine biosynthesis 247
Microbial physiology 248
Motility and chemotaxis 249
Gene mapping 250
Conjugational analysis 250
Gradient of transfer 250
Interrupted mating 252
Co transformation and co transduction 252
Three factor cross 255
Molecular techniques for gene mapping 258
Gene libraries 258
Restriction mapping and pulsed field gel
electrophoresis 259
DNA sequence determination 262
Genome sequencing 263
Physical and genetic maps 264
Deletions and insertions 266
Transposon mutagenesis 266
PCR analysis of mutants 268
Gene replacement 268
Site directed mutagenesis 269
Analysis of gene expression 270
Western blots 270
RNA levels: Northern blots and RT PCR 270
Reporter genes 271
Detection of virulence genes 273
Diagnosis and epidemiology 275
Gene probes for detection of pathogens 275
Diagnostic use of PCR 275
Molecular epidemiology; RFLP analysis 275
Genetic fingerprinting 277
Human genetic diseases and gene therapy 277
Conclusion 278
Appendix A FURTHER READING 279
Appendix B ABBREVIATIONS USED 283
Appendix C GLOSSARY 285
xii Molecular Genetics of Bacteria
Appendix D ENZYMES 297
Appendix E GENES 299
Index 303
|
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| spelling | Dale, Jeremy W. Verfasser aut Molecular genetics of bacteria Jeremy W. Dale 3. ed. Chichester [u.a.] Wiley 1998 XIV, 310 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bacteriofagen gtt Bacteriën gtt Genetica gtt Genetic Engineering Genetic engineering Genetics, Microbial Microbial genetics Molecular Biology Molecular genetics Gentechnologie (DE-588)4071722-7 gnd rswk-swf Bakterien (DE-588)4004296-0 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Bakterien (DE-588)4004296-0 s Molekulargenetik (DE-588)4039987-4 s DE-604 Gentechnologie (DE-588)4071722-7 s HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007962733&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dale, Jeremy W. Molecular genetics of bacteria Bacteriofagen gtt Bacteriën gtt Genetica gtt Genetic Engineering Genetic engineering Genetics, Microbial Microbial genetics Molecular Biology Molecular genetics Gentechnologie (DE-588)4071722-7 gnd Bakterien (DE-588)4004296-0 gnd Molekulargenetik (DE-588)4039987-4 gnd |
| subject_GND | (DE-588)4071722-7 (DE-588)4004296-0 (DE-588)4039987-4 |
| title | Molecular genetics of bacteria |
| title_auth | Molecular genetics of bacteria |
| title_exact_search | Molecular genetics of bacteria |
| title_full | Molecular genetics of bacteria Jeremy W. Dale |
| title_fullStr | Molecular genetics of bacteria Jeremy W. Dale |
| title_full_unstemmed | Molecular genetics of bacteria Jeremy W. Dale |
| title_short | Molecular genetics of bacteria |
| title_sort | molecular genetics of bacteria |
| topic | Bacteriofagen gtt Bacteriën gtt Genetica gtt Genetic Engineering Genetic engineering Genetics, Microbial Microbial genetics Molecular Biology Molecular genetics Gentechnologie (DE-588)4071722-7 gnd Bakterien (DE-588)4004296-0 gnd Molekulargenetik (DE-588)4039987-4 gnd |
| topic_facet | Bacteriofagen Bacteriën Genetica Genetic Engineering Genetic engineering Genetics, Microbial Microbial genetics Molecular Biology Molecular genetics Gentechnologie Bakterien Molekulargenetik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007962733&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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