Genome analysis: a laboratory manual 1 Analyzing DNA
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Plainview, NY
Cold Spring Harbor Laboratory Press
1997
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXVII, 675 S. Ill., graph. Darst. |
| ISBN: | 0879694963 |
Internformat
MARC
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| 008 | 980121s1997 ad|| |||| 00||| eng d | ||
| 020 | |a 0879694963 |9 0-87969-496-3 | ||
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| 041 | 0 | |a eng | |
| 049 | |a DE-20 |a DE-355 |a DE-11 |a DE-188 | ||
| 245 | 1 | 0 | |a Genome analysis |b a laboratory manual |n 1 |p Analyzing DNA |c series ed. Eric D. Green ... vol. eds. Bruce Birren ... |
| 264 | 1 | |a Plainview, NY |b Cold Spring Harbor Laboratory Press |c 1997 | |
| 300 | |a XXVII, 675 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 7 | |a Genome Analysis |2 cabt | |
| 700 | 1 | |a Green, Eric D. |e Sonstige |4 oth | |
| 700 | 1 | |a Birren, Bruce |e Sonstige |4 oth | |
| 773 | 0 | 8 | |w (DE-604)BV011728062 |g 1 |
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Datensatz im Suchindex
| _version_ | 1804126267989032960 |
|---|---|
| adam_text | Contents
VOLUME 1
Preface, xv
Acknowledgments, xix
Contributors, xxi
Abbreviations and Acronyms, xxiii
1 Purifying and Analyzing Genomic DNA, 1
OVERVIEW OF GENOMIC DNA ANALYSIS, 2
Historical Perspective, 2
Issues of Contamination and Safety, 2
ISOLATION OF GENOMIC DNA, 3
DNA FROM MAMMALIAN SOURCES, 4
PROTOCOLS
DNA Isolation from Tissue culture Cell Lines Growing in Suspension, 6
Transforming Lymphoblasts with EBV, 9
Maintaining B95 8 and IA 3 EBV producing Cell Lines, 11
DNA Isolation from Adherent Fibroblasts Using Trypsin, 12
DNA Isolation from Adherent Fibroblasts ( Sliming ), 13
DNA Isolation from Peripheral Blood, 14
DNA Isolation from Fresh and Frozen Tissues, 15
DNA Isolation from a Mammalian Model Organism: The Mouse, 16
DNA FROM ANIMAL MODEL ORGANISMS, 17
PROTOCOLS
DNA Isolation from D. melanogaster, 18
DNA Isolation from C. elegans, 20
DNA FROM PLANTS, 23
PROTOCOL
DM4 Isolation from Arabidopsis thaliana, 24
Vi Contents
DNA FROM YEAST, 26
PROTOCOLS
DNA Isolation from S. cerevisiae, 27
DNA Isolation from S. pombe, 30
DNA FROM BACTERIA, 34
PROTOCOL
DNA Isolation from E. coli, 35
TROUBLESHOOTING FOR GENOMIC DNA ISOLATION, 37
Degradation and Fragmentation of the DNA, 37
Poor Recovery of DNA, 37
Contamination of the DNA with Proteins, 37
Contamination of the DNA with RNA, 37
SOUTHERN BLOTTING, 38
DIGESTION OF DNA WITH RESTRICTION ENZYMES, 39
PROTOCOL
Digesting Genomic DNA with Restriction Enzymes, 43
ELECTROPHORETIC SEPARATION OF DIGESTION PRODUCTS, 45
PROTOCOL
Size Fractionating Digested DNA on Agarose Gels, 47
TRANSFER OF DNA ONTO SOLID SUPPORTS, 49
PROTOCOL
Transferring Size fractionated DNA onto Solid Supports, 50
HYBRIDIZATION OF PROBES TO IMMOBILIZED DNA, 56
PROTOCOL
Gel Purification of DNA Fragments for Use as Probes, 57
RADIOLABELING DNA PROBES, 59
PROTOCOLS
Radiolabeling Probes by Random Priming with Hexameric Oligonucleotides, 60
End labeling Oligonucleotide Probes, 62
BLOCKING REPETITIVE DNA SEQUENCES, 64
PROTOCOLS
Preannealing Probes to Cot 1 Blocking DNA, 65
Preannealing Probes to Genomic Blocking DNA, 67
DETECTING UNIQUE SEQUENCES ON MEMBRANES BY USING RADIOLABELED
PROBES, 68
PROTOCOLS
Hybridization Using Probes Labeled by Random Priming, 70
Hybridization Using End labeled Oligonucleotide Probes, 75
USE OF NONRADIOACTIVE PROBES FOR HYBRIDIZATION, 77
Biotinylation of Probe DNA, 77
Membrane Preparation, 77
Hybridization and Washing, 77
Detection of Chemiluminescence, 77
TROUBLESHOOTING FOR SOUTHERN BLOTTING, 78
Problems with the DNA Sample, 78
Problems with the Gel, 79
Problems with Transferring the DNA onto the Solid Support, 79
Problems with Hybridizing Radioactive or Nonradioactive Probes to the DNA, 79
Contents vii
2 Preparation, Manipulation, and Mapping of HMW DNA, 83
PRINCIPLES OF PREPARING AND MANIPULATING HMW DNA, 84
General Characteristics of HMW DNA, 84
Principles in the Preparation of HMW DNA, 84
Assessing the Quality of HMW DNA, 89
Quantitating HMW DNA, 89
PREPARATION OF HMW MAMMALIAN DNA, 91
PROTOCOLS
Preparing Mononuclear Cells from Whole Blood by Centrifugation on Histopaque, 93
Isolating White Blood Cells from Whole Blood by Sedimentation After Lysis of Red Blood Cells, 95
Preparing HMW DNA in Agarose Starting from Frozen Whole Blood, 97
Preparing Cells from Tissues, 99
Preparing Cells from Tissue Culture, 100
Preparing Cells from Frozen Semen, 101
Using Proteinase K to Prepare HMW Mammalian DNA Embedded in Agarose Plugs, 103
Using LIDS to Prepare HMW Mammalian DNA Embedded in Agarose Plugs, 106
Preparing HMW Mammalian DNA in Agarose Beads, 109
Purifying HMW Mammalian DNA in Liquid Form, 112
PREPARATION OF HMW YEAST DNA, 117
PROTOCOLS
Growing S. cerevisiae Cells, 119
Using Proteinase K to Prepare HMW Yeast DNA Embedded in Agarose Plugs, 121
Using LIDS to Prepare HMW Yeast DNA Embedded in Agarose Plugs, 125
Preparing HMW Yeast DNA in Agarose Beads, 128
Purifying HMW Yeast DNA in Liquid Form, 132
PREPARATION of HMW DNA FROM BACTERIAL CLONES, 135
PROTOCOLS
Preparing HMW Bacterial DNA Embedded in Agarose Plugs, 136
Preparing HMW Bacterial DNA in Agarose Beads, 139
DIGESTION OF HMW DNA WITH RESTRICTION ENZYMES, 142
COMPLETE DIGESTION OF HMW DNA SAMPLES, 143
PROTOCOLS
Complete Digestion of HMW DNA Embedded in Agarose Plugs, 145
Complete Digestion of HMW DNA Embedded in Agarose Beads, 148
Complete Digestion of HMW DNA in Liquid Form, 151
PARTIAL DIGESTION OF HMW DNA SAMPLES, 152
PROTOCOLS
Partial Digestion by Varying the Concentration of Restriction Enzyme, 157
Partial Digestion by Varying the Length of the Incubation with the Restriction Enzyme, 160
Partial Digestion by Varying the Mg++ Concentration, 162
Partial Digestion by Methylase Competition, 165
ANALYSIS AND PREPARATIVE SIZE FRACTIONATION OF PARTIAL DIGESTION
PRODUCTS, 169
PROTOCOLS
Size Fractionation of Partial Digestion Fragments Using a Sucrose Density Gradient, 171
Concentration of Liquid HMW DNA Fragments by Vacuum Dialysis, 175
Vl ii Contents *
LONG RANGE RESTRICTION MAPPING, 179 ™
General Principles for Long range Restriction Mapping, 179
Selection of Enzymes for Generating Large DNA Fragments, 181 I
Mapping Reagents, 182 ¦
Construction of a Long range Restriction Map, 183
Troubleshooting for Long range Restriction Mapping, 184 ¦
SITE SPECIFIC CLEAVAGE OF DNA USING RARE CLEAVAGE, 186 ¦
General Principles of RARE Cleavage, 186
Applications of RARE Cleavage, 189 I
PROTOCOLS FOR RARE CLEAVAGE, 190 ¦
PROTOCOLS
RARE Cleavage with EcoRI or Alu/ in Low percentage agarose Plugs, 193 ¦
RARE Cleavage with EcoRI in Diced Agarose Plugs, 198 m
RARE Cleavage with EcoRI in Agarose Beads, 202
Potential Problems and Troubleshooting for RARE Cleavage, 206 fl
PULSED FIELD GEL ELECTROPHORESIS, 208 I
Conventional Agarose Gel Electrophoresis and the Need for PFGE, 208
Setting Up the Components of a PFGE System, 210 M
Achieving Optimal Separation of DNAs, 210 |
Selecting Appropriate Electrophoresis Conditions, 215
Estimating DNA Fragment Sizes on a PFG, 218 M
Potential Problems with PFGE and Troubleshooting, 221 |
SETTING UP AND RUNNING A PFG, 223
PROTOCOLS m
Preparing the Gel and Loading DNA Samples in Agarose Beads or Plugs, 224 |
Casting the Gel Around DNA Samples in Agarose Plugs, 227
Preparing the Gel and Loading Liquid DNA Samples or Samples in Agarose Beads in a *m
Submerged Gel, 228 ¦
Running and Staining the Gel, 230
PREPARATIVE PFGE FOR RECOVERY OF HMW DNA, 232 —
RECOVERING HMW DNA FROM A PREPARATIVE PFG, 233 I
PROTOCOLS
Recovery of DNA from PFGsfor Applications That Do Not Require Intact HMW DNA, 234 _
Recovery of Intact HMW DNA from PFGs, 238 I
Troubleshooting for the Recovery of Intact HMW DNA, 243
Handling and Treatment of Gel purified HMW DNA for Transfer into Mammalian
Cells, 243 ¦
3 PCR in Genome Analysis, 249 ¦
BASIC PRINCIPLES AND COMPONENTS OF PCR, 250 Wk
A Simple PCR Protocol, 251 V
Oligonucleotide Primers, 251
Sample Preparation, 252 d|
Thermostable DNA Polymerases, 253 Jm
Instrumentation, 254
Contents i
BASIC PROTOCOLS NEEDED FOR PREPARING KEY COMPONENTS OF THE
PCR ASSAY, 256
PROTOCOLS
Basic DNA Amplification, 257
Desalting Oligonuckotides by Precipitation, 259
Desalting Oligonuckotides on Nensorb Affinity Columns, 260
Rapid Preparation of Template DNA from Mammalian Cells, 262
OPTIMIZING PCR ASSAYS, 264
Selecting New Primers, 264
Modifying Thermal Cycling Conditions, 264
Altering the Mg++ Concentration, 265
Altering the pH, 265
Altering Other Reaction Components, 265
Troubleshooting, 265
METHODS FOR IMPROVING PCR, 267
Hot Start PCR, 267
Nested PCR, 267
Touch down PCR, 267
Booster PCR, 267
DETECTING AND CHARACTERIZING PCR PRODUCTS, 268
Detection Schemes, 268
Quantitative PCR, 269
Cloning PCR Products, 270
Amplification Using Mixed Oligonucleotides as Primers, 271
In Vitro Gene Assembly by PCR, 272
RT PCR for Amplification from RNA, 273
In Situ PCR, 274
Amplification of Large DNA Segments (Long range PCR), 275
PCR based Fingerprinting, 277
Multiplex PCR, 281
PROTOCOLS FOR DETECTING AND CHARACTERIZING PCR PRODUCTS, 282
PROTOCOLS
Long range PCR Protocol, 283
DD PCR Protocol, 285
CONTAMINATION IN PCR ASSAYS, 291
Sources of Contamination, 291
Avoidance of Contamination, 291
Elimination of Contamination, 291
FURTHER READING, 293
4 Fluorescence based DNA Sequencing, 301
OVERVIEW OF DNA SEQUENCING METHODS, 303
PREPARING SUBCLONED DNA TEMPLATES, 306
PROTOCOLS
Preparing Single stranded Bacteriophage M13 DNA Using PEG and Phenol, 308
X Contents
Preparing Single stranded Bacteriophage Ml 3 DNA Using Detergent, 310
Preparing Single stranded Phagemid DNA, 312
Purifying Double stranded Plasmid DNA by Alkaline Lysis, 314
Purifying Double stranded Plasmid DNA in a 96 well Format Using an Alkaline Lysis Kit, 317
Denaturing Double stranded DNA for Sequencing, 319
Denaturing Double stranded DNA and Annealing Primer to Template for Sequencing, 320
PREPARING SEQUENCING TEMPLATES BY PCR, 321
PROTOCOLS
Amplifying Subclone Inserts by PCR and Precipitating PCR Products with PEG to Purify Sequencing
Template, 326
Treating PCR Products with Exonuclease I plus Shrimp Alkaline Phosphatase to Purify Sequencing
Template, 329
Preparing Single stranded Template DNA by Asymmetric PCR, 330
Preparing Single stranded Template DNA from PCR Products Using Bacteriophage T7 Gene 6
Exonuclease, 332
Preparing Single stranded Template DNA from PCR Products Using Bacteriophage X Exonuclease, 334
Purifying Single stranded Template DNA from PCR Products Using Streptavidin linked Magnetic
Beads, 336
SEQUENCING PRIMERS, 339
FLUORESCENCE BASED DNA SEQUENCING REACTIONS, 340
PROTOCOLS
Sequencing Reactions Using Fluorescent Dye labeled Primers and Sequenase DNA Polymerase, 342
Sequencing Reactions Using Fluorescent Dye labeled Primers and ThermoSequenase DNA
Polymerase, 346
Sequencing Reactions Using Fluorescent Dye labeled Primers and SequiTherm DNA Polymerase, 350
Sequencing Reactions Using Fluorescent Dye labeled Terminators and ThermoSequenase DNA
Polymerase, 355
Sequencing Reactions Using Fluorescent Dye labeled Terminators and AmpliTaq DNA Polymerase
FS, 358
Removing Unincorporated Dye labeled Terminators after Cycle Sequencing by Using Sephadex in Filter
Plates, 359
Removing Unincorporated Dye labeled Terminators after Cycle Sequencing by Using Centri Sep
Columns, 361
PREPARING, LOADING, AND RUNNING GELS ON FLUORESCENCE BASED
SEQUENCING INSTRUMENTS, 363
PROTOCOLS
Washing and Assembling Gel Plates, 364
Pouring the Gel, 365
Loading and Running the Gel, 368
TROUBLESHOOTING FOR FLUORESCENCE BASED DNA SEQUENCING, 370
Problems Caused by Software, 370
Problems Caused by Templates or by Pipetting Errors, 372
Problems Caused by DNA Structure, 376
STRATEGIES FOR DETERMINING CONTIGUOUS SEGMENTS OF DNA
SEQUENCE, 379
Types of Strategies, 379
Choosing a Strategy, 379
PRIMER DIRECTED SEQUENCING, 384
Contents xi
DELETIONAL SEQUENCING, 385
PROTOCOLS
Digestion of Template DNA with Restriction Enzymes, 387
Digestion of Template DNA with Exonuclease III and Mung bean Nudease, 389
Religation ofSubdones That Have Deletions and Transformation ofE. coli, 391
SEQUENCING cDNA CLONES, 392
5 Shotgun Sequencing
OVERVIEW OF SHOTGUN SEQUENCING, 398
LIBRARY CONSTRUCTION, 399
PREPARATION OF TARGET DNA, 402
PROTOCOLS
Purification of Bacterial Clone DNA on CsCl Gradients, 404
Preparation of Bacterial Clone DNA by Gentle Alkaline Lysis, 408
Purification of Agarose embedded HMW Yeast DNA for Isolation of YAC DNA by Preparative
PFGE, 410
FRAGMENTATION OF TARGET DNA, 414
PROTOCOLS
Random Fragmentation by Low pressure Shearing in a French Pressure Cell, 415
Random Fragmentation by Sonication, 418
Random Fragmentation by Nebulization, 421
Random Fragmentation with DNase I, 424
Random Fragmentation by Partial Digestion with Sau3A/, 426
END REPAIR AND SIZE FRACTIONATION OF FRAGMENTED DNA, 428
PROTOCOLS
Using DNA Polymerases and Polynucleotide Kinase to Repair Fragment Ends, 429
Using Mung bean Nudease to Repair Fragment Ends, 431
Size Fractionation of Repaired DNA Fragments, 432
CLONING FRAGMENTED DNA, 434
PROTOCOLS
Vector Preparation, 436
Ligation of Vector DNA and Insert DNA, 439
Transforming Bacteria with Ligated DNA by Using Electroporation, 441
Preparation of Cells Competent for Electroporation, 441
Electroporation, 442
Transforming Bacteria with Ligated DNA by Using CaCl2 and Heat Shock, 444
Preparation of Competent Cells, 444
Transformation, 445
INITIAL SEQUENCE ACQUISITION, DATA PROCESSING, AND SEQUENCE
ASSEMBLY, 447
SEQUENCE FINISHING, 448
Subclone directed Closure, 448
Primer directed Closure, 449
Resolving Ambiguities, 450
Resolving Repeats, 456
Xii Contents
6 Transposon mediated DNA Sequencing, 455
COMPARISON OF SHOTGUN SEQUENCING AND TRANSPOSON MEDIATED
SEQUENCING, 456
TRANSPOSONS USED FOR DNA SEQUENCING, 456
y5 TRANSPOSON MEDIATED DNA SEQUENCING, 459
PREPARING LIBRARIES OF SUBCLONES WITH 3 4 KB INSERTS FROM LARGE
GENOMIC CLONES, 460
PROTOCOLS
Sonication, End Repair, and Size Selection of3 4 kb Subclone Inserts, 463
Preparation of stXI digested pOT2A Vector DNA, 466
Addition of BstXI Adapters to Size selected Insert DNA and Ligation to BstXI digested Vector DNA, 468
BUILDING INITIAL SUBCLONE TILING PATHS, 472
PROTOCOL
Building Initial Subclone Tiling Paths from End Sequences, 477
SEQUENCING SUBCLONES BY USING THE y5 TRANSPOSON, 483
PROTOCOLS
Mobilizing Transposons into Subclones, 487
Mapping yb Transposon insertion Sites by Using PCR, 489
Sequencing the Minimal Set ofTransposon bearing Templates, 491
Assembling Subclone Sequences, 492
EXTENDING SUBCLONE TILING PATHS AND ASSEMBLING SUBCLONE
SEQUENCES INTO LARGER CONTIGS, 494
PROTOCOL
Extending Stalled Contigs in the Tiling Path and Assembling the Finished Genomic Clone Sequence, 495
IN VITRO TY1 TRANSPOSON MEDIATED DNA SEQUENCING, 498
INTEGRATION OF ARTIFICIAL TRANSPOSONS INTO TARGET DNA IN VITRO, 500
PROTOCOLS
Preparation of AT 2 for In Vitro Integration, 503
Purification ofVLPsforln Vitro Integration, 506
Growing Gal Tyl Yeast Strains and Inducing the GAL1 Promoter, 506
Recovering and Lysing Cells, 507
Centrifuging the Lysate on a Sucrose Gradient and Identifying Fractions with
Integrase Activity, 510
In Vitro Integration of AT 2 into Target DNA, 512
Recovery of Recombinant Plasmids by Electroporation and Selection with Antibiotics, 514
SEQUENCING FROM MAPPED OR RANDOMLY CHOSEN TRANSPOSON INSERTION
SITES, 517
PROTOCOLS
Mapping Tyl Transposon insertion Sites by Using PCR, 521
Restriction Mapping of Tyl Transposon insertion Sites, 523
Sequencing DNA from Sites of AT 2 Insertion, 527
TROUBLESHOOTING FOR IN VITRO TY1 TRANSPOSON MEDIATED
SEQUENCING, 529
Evaluation of Integration Efficiency, 529
Optimization of Molar Ratios of Transposon DNA to Target DNA, 529
Cotransformation with Parental Target Plasmids and Recombinants, 529
Contamination by Recombinants from Previous Experiments, 530
Contents XM
7 Computational Analysis of DNA and Protein Sequences, 533
INTERNET BASICS, 534
Electronic Mail, 534
File Transfer Protocol, 535
Gopher, 537
The World Wide Web, 538
Electronic Publishing, 541
Specialized Client Server Applications, 542
SEQUENCE ANALYSIS, 543
Searching Databases for Similarities, 543
Sequence Databases, 543
The BLAST Family of Programs: Uses and Examples, 544
DNA Query Sequences, 546
Protein Query Sequences, 554
Confounding Subsequences and Query Masking, 555
Additional BLAST Options, 5 58
INTEGRATED INFORMATION RETRIEVAL, 560
The Entrez System, 561
Specialized Data Sets, 566
C. elegans, 566
D. melanogaster, 573
MULTIPLE ALIGNMENT, SEQUENCE MOTIFS, AND STRUCTURE
INFERENCE, 574
Multiple Alignment, 574
Sequence Motifs, 575
Structure Prediction and Protein Modeling, 576
SUBMITTING DATA TO PUBLIC DATABASES, 579
Preparing a New Submission, 580
Updates and Corrections, 581
Special Arrangements for Large Projects, 583
APPENDICES
1 Common Reagents, 589
Standard Stock Solutions, 590
Molarities of Concentrated Acids and Bases, 597
Common Laboratory Solutions, 598
Electrophoresis Buffers, Dyes, and Gel loading Solutions, 601
Media, 605
Antimicrobial Agents, 610
XIV Contents
2 Basic Procedures, 613
Quantitation of Cell Concentration, 614
Standard Methods Used for Isolating DNA, 617
Extraction of DNA Samples with Organic Chemicals, 617
Concentration of DNA Samples by Precipitation with Alcohols, 621
Quantitation of DNA, 624
Dialysis of DNA, 628
Assessing the Extent of Radiolabeling in DNA Probes by Precipitation with TCA, 630
Dilution and Storage of Oligonucleotides, 632
Storage and Shipment of Biological Samples, 633
3 Safety Cautions, 639
4 Useful Facts, 647
Conversion Factors, 648
Genome Comparisons, 649
Average Sizes of DNA Fragments Generated by Cleavage with Restriction Enzymes, 650
Single letter Abbreviations for Amino Acids, 651
Genetic Code, 652
Codon Facts, 653
Isotope Information, 654
5 Suppliers, 655
Index, 657
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| indexdate | 2024-07-09T18:14:45Z |
| institution | BVB |
| isbn | 0879694963 |
| language | English |
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| spelling | Genome analysis a laboratory manual 1 Analyzing DNA series ed. Eric D. Green ... vol. eds. Bruce Birren ... Plainview, NY Cold Spring Harbor Laboratory Press 1997 XXVII, 675 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Genome Analysis cabt Green, Eric D. Sonstige oth Birren, Bruce Sonstige oth (DE-604)BV011728062 1 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007910470&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Genome analysis a laboratory manual Genome Analysis cabt |
| title | Genome analysis a laboratory manual |
| title_auth | Genome analysis a laboratory manual |
| title_exact_search | Genome analysis a laboratory manual |
| title_full | Genome analysis a laboratory manual 1 Analyzing DNA series ed. Eric D. Green ... vol. eds. Bruce Birren ... |
| title_fullStr | Genome analysis a laboratory manual 1 Analyzing DNA series ed. Eric D. Green ... vol. eds. Bruce Birren ... |
| title_full_unstemmed | Genome analysis a laboratory manual 1 Analyzing DNA series ed. Eric D. Green ... vol. eds. Bruce Birren ... |
| title_short | Genome analysis |
| title_sort | genome analysis a laboratory manual analyzing dna |
| title_sub | a laboratory manual |
| topic | Genome Analysis cabt |
| topic_facet | Genome Analysis |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007910470&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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