Methods in muscle biology:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
San Diego, Calif. [u.a.]
Acad. Press
1997
|
| Schriftenreihe: | Methods in cell biology
52 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 493 S. Ill., graph. Darst. |
| ISBN: | 0125641540 0122381904 |
Internformat
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| 300 | |a XVI, 493 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in cell biology |v 52 | |
| 650 | 4 | |a Fibres musculaires | |
| 650 | 4 | |a Muscles - Aspect moléculaire | |
| 650 | 4 | |a Muscles - Cytologie - Technique | |
| 650 | 4 | |a Myogénèse | |
| 650 | 7 | |a Spieren |2 gtt | |
| 650 | 4 | |a Cell Differentiation |x physiology | |
| 650 | 4 | |a Gene Expression |x genetics | |
| 650 | 4 | |a Muscles |x Physiology | |
| 650 | 4 | |a Muscles |x Research |x Methodology | |
| 650 | 4 | |a Muscles |x physiology | |
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Datensatz im Suchindex
| _version_ | 1804126242651242496 |
|---|---|
| adam_text | CONTENTS
Contributors xi
Preface xv
PART I Embryological Analysis of Myogenesis
1. Avian Somite Transplantation: A Review of Basic Methods
Charles P. Ordahl and Bodo Christ
I. Introduction 4
II. Materials 5
III. Methods 11
IV. Summary and Concluding Remarks 25
References 26
2. Myogenesis in the Mouse Embryo
Margaret Buckingham and Giulio Cossu
I. Introduction 29
II. Manipulation of Genes in the Mouse 31
III. Analysis of Gene Expression in the Mouse Embryo 38
IV. Experimental Embryology 43
V. Perspectives 48
References 50
3. Myogenesis in Xenopus Embryos
John B. Gurdon, Patrick Lemaire, and Timothy J. Mohun
I. Fate Maps for Muscle 53
II. Embryo Manipulations 55
III. Molecular Markers of Muscle Differentiation 59
IV. Studies of Xenopus Heart Formation 62
References 63
4. Zebrafish: Genetic and Embryological Methods in a Transparent
Vertebrate Embryo
Mark C. Fishman, Didier Y. R. Stainier, Roger E. Breitbart, and Monte Westerfield
I. Introduction 68
II. Genetics 68
III. Heart Development 70
vi Contents
IV. Skeletal Muscle Development 72
References 79
PART II Myogenesis in Cell Culture
5. Skeletal Muscle Cultures
Craig Neville, Nadia Rosenthal, Michael McGrew, Natalia Bogdanova,
and Stephen Hauschka
I. Introduction 85
II. Muscle Cell Cultures 86
III. Summary and Future Prospects 113
References 114
6. Avian Cardiac Progenitors: Methods for Isolation, Culture, and Analysis
of Differentiation
Maureen Gannon and David Bader
I. Establishment of Embryonic Axis 118
II. Pregastrulation Location of Cardiac Progenitors 119
III. Postgastrulation Location of Cardiac Progenitors 119
IV. Removal of Embryos from the Egg 120
V. Removal of Cardiac Progenitors 121
VI. Removal of Endoderm 122
VII. Culturing Whole Embryos 124
VIII. Culturing Explants of Cardiogenic Mesoderm 125
IX. Culturing Primary Cardiomyocytes 126
X. Analysis of Differentiation 127
References 131
7. Vascular Smooth Muscle Cell Cultures
Rebecca R. Pauly, Claudio Bilato, Linda Cheng, Robert Monticone, and Michael T. Crow
I. Introduction 133
II. Establishment of Cell Cultures 136
III. Characterization of Cultured Vascular Smooth Muscle Cells 141
IV. Specialized Methods for Human Tissues 147
V. Specialized Methods to Maintain the Differentiated State 150
VI. Conclusions and Perspectives 152
References 153
8. Skeletal Muscle Satellite Cell Cultures
Ronald E. Allen, Constance J. Temm Grove, Shannon M. Sheehan, and Glenna Rice
I. Introduction 155
II. Physiological or Developmental Background of Subjects 157
III. Monolayer Mass Cultures 158
Contents vii
IV. Isolated Single Fiber Cultures 170
V. Advantages and Disadvantages of Each Culture System 173
VI. Conclusions 174
References 175
PART III Viral and Cellular Gene Delivery Systems in Muscle
9. Retroviral Gene Delivery
Mark J. Federspiel and Stephen H. Hughes
I. Introduction 179
II. Vector Construction and Virus Propagation 191
III. Avian Model 196
IV. ALV/Receptor Mouse Model 201
V. Summary/Reprise 207
References 209
10. The Use of Replication Defective Retroviruses for Cell Lineage Studies
of Myogenic Cells
Donald A. Fischman and Takashi Mikawa
I. Introduction 215
II. Basic Principles 216
III. Practical Procedures 218
IV. Application of Retroviruses for Muscle Lineage Analyses in Chicken Embryos 223
References 225
11. Adenoviral Gene Delivery
Tluerry Ragot, Paule Opolon, and Michel Perricaudet
I. Introduction to Research 230
II. Recombinant Adenovirus Design 234
III. Practical Methods 238
IV. Applications of Adenoviral Gene Transfer to Muscle 241
V. Current Problems and Future Prospects 248
VI. Conclusion 256
References 256
12. Methods for Myoblast Transplantation
Thomas A. Rando and Helen M. Blau
I. Introduction 261
II. Preparation of Cells 262
III. Labeling of Myoblasts in Vitro by Retroviral Mediated Gene Transfer 264
IV. Transplantation Techniques 266
V. Evaluation of Transplant 268
viii Contents
VI. Concluding Remarks 271
References 271
PART IV Molecular Analysis of Muscle Structure and Function
13. Structure Function Analysis of Cytoskeletal/Contractile Proteins in
Avian Myotubes
H. Lee Sweeney and Huisheng Feng
I. Introduction 275
II. Muscle Cultures 276
III. Other Considerations 279
References 281
14. Functional and Structural Approaches to the Study of
Excitation Contraction Coupling
Kurt G. Beam and Clara Franzini Armstrong
I. Functional and Structural Approaches to the Identification of e—c 284
Coupling Components
II. The Dysgenic Muscle Model 296
III. Conclusions 305
References 305
15. Adenovirus Mediated Myofilament Gene Transfer into Adult
Cardiac Myocytes
Margaret West/all, Elizabeth Rust, Fans Albayya, and Joseph M. Metzger
I. Introduction 307
II. Isolation of Adult Cardiac Myocytes and Adenovirus Mediated Gene Transfer 309
III. Production of Recombinant Adenovirus Vectors 313
IV. Analysis of Myofilament Protein Expression and Incorporation in Adenovirus 317
Infected Ventricular Myocytes
V. Summary and Future Directions 320
References 321
16. In Vivo Approaches to Neuromuscular Structure and Function
RitaJ. Balke Gordon
I. Introduction 323
II. In Vivo Analysis of Skeletal Muscle and Neuromuscular Innervation 324
III. Manipulation of Motor Neurons, Muscle Fibers, and Synapses in Vivo 337
IV. In Vivo Observations and Manipulations of Muscle Fibers and Neuromuscular 338
Innervation: Practical Considerations
V. In Vivo Approaches: New Insights into Neuromuscular Junction Plasticity 339
VI. Summary 346
References 346
Contents ix
17. Molecular Diversity of Myofibrillar Proteins: Isoforms Analysis at the
Protein and mRNA Level
Stefano Schiaffino and Giovanni Salviati
I. Myofibrillar Protein Isoforms in Muscle Tissues 349
II. Multiple Approaches to Isoform Analysis 350
III. Electrophoretic Techniques and Immunoblotting 356
IV. Immunohistochemistry and in Situ Hybridization 359
V. Other Techniques for mRNA Analysis 361
VI. Isoform Analysis and Physiological Studies in Single Muscle Fibers 364
VII. Summary and Perspectives 365
References 367
PART V Molecular Genetic Analysis of Muscle Gene Regulation
18. Transgenic Mice: Production and Analysis of Expression
Alexander Faerman and Moshe Shani
I. Introduction 374
II. Production of Stable Transgenic Mice 375
III. Production of Transient Transgenics 383
IV. Analysis of Expression at the RNA Level 384
V. Analysis of Expression at the Protein Level 397
VI. Future Directions 400
References 401
19. DNA Transfection of Cultured Muscle Cells
Craig Neville, Nadia Rosenthal, and Stephen Hauschka
I. Introduction 405
II. Transient Transfections 406
III. Stable Transfections 412
IV. Identification of as Acting Control Regions with Reporter Assays 415
V. Summary and Future Prospects 421
References 422
20. DNA and Adenovirus Mediated Gene Transfer into Cardiac Muscle
Alyson Kass Eisler and Leslie A. Leinwand
I. Introduction 423
II. Methods for Cardiac Injection 426
III. Homogenization of Tissue and Assays 428
IV. Experimental Design 430
V. Future Directions 433
References 435
X Contents
21. Nuclear DNA Binding Proteins
Kristen L. Kucharczuk and David J. Goldhamer
I. Introduction 440
II. Nuclear Extract Preparation: Overview and Important Parameters 441
III. Nuclear Extract Protocol 442
IV. Electromobility Shift Assays: Overview and Important Parameters 446
V. EMSA Protocol 450
VI. DNase I Protection Assays: Overview and Important Parameters 454
VII. DNase I Protection Assay Protocol 458
VIII. Relevance of the EMSA and DNase I Protection Assay to Muscle Research 466
References 471
Index 473
Volumes in Series 489
|
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| indexdate | 2024-07-09T18:14:21Z |
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| isbn | 0125641540 0122381904 |
| language | English |
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| physical | XVI, 493 S. Ill., graph. Darst. |
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| publishDateSearch | 1997 |
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| publisher | Acad. Press |
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| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Methods in muscle biology ed. by Charles P. Emerson ... San Diego, Calif. [u.a.] Acad. Press 1997 XVI, 493 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 52 Fibres musculaires Muscles - Aspect moléculaire Muscles - Cytologie - Technique Myogénèse Spieren gtt Cell Differentiation physiology Gene Expression genetics Muscles Physiology Muscles Research Methodology Muscles physiology Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Muskel (DE-588)4040895-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Muskelentwicklung (DE-588)4170853-2 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf Muskel (DE-588)4040895-4 s Molekulargenetik (DE-588)4039987-4 s Methode (DE-588)4038971-6 s DE-604 Zellkultur (DE-588)4067547-6 s Muskelentwicklung (DE-588)4170853-2 s Molekularbiologie (DE-588)4039983-7 s Emerson, Charles Phillips edt Methods in cell biology 52 (DE-604)BV002534878 52 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007893137&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Methods in muscle biology Methods in cell biology Fibres musculaires Muscles - Aspect moléculaire Muscles - Cytologie - Technique Myogénèse Spieren gtt Cell Differentiation physiology Gene Expression genetics Muscles Physiology Muscles Research Methodology Muscles physiology Molekularbiologie (DE-588)4039983-7 gnd Molekulargenetik (DE-588)4039987-4 gnd Muskel (DE-588)4040895-4 gnd Methode (DE-588)4038971-6 gnd Muskelentwicklung (DE-588)4170853-2 gnd Zellkultur (DE-588)4067547-6 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4039987-4 (DE-588)4040895-4 (DE-588)4038971-6 (DE-588)4170853-2 (DE-588)4067547-6 |
| title | Methods in muscle biology |
| title_auth | Methods in muscle biology |
| title_exact_search | Methods in muscle biology |
| title_full | Methods in muscle biology ed. by Charles P. Emerson ... |
| title_fullStr | Methods in muscle biology ed. by Charles P. Emerson ... |
| title_full_unstemmed | Methods in muscle biology ed. by Charles P. Emerson ... |
| title_short | Methods in muscle biology |
| title_sort | methods in muscle biology |
| topic | Fibres musculaires Muscles - Aspect moléculaire Muscles - Cytologie - Technique Myogénèse Spieren gtt Cell Differentiation physiology Gene Expression genetics Muscles Physiology Muscles Research Methodology Muscles physiology Molekularbiologie (DE-588)4039983-7 gnd Molekulargenetik (DE-588)4039987-4 gnd Muskel (DE-588)4040895-4 gnd Methode (DE-588)4038971-6 gnd Muskelentwicklung (DE-588)4170853-2 gnd Zellkultur (DE-588)4067547-6 gnd |
| topic_facet | Fibres musculaires Muscles - Aspect moléculaire Muscles - Cytologie - Technique Myogénèse Spieren Cell Differentiation physiology Gene Expression genetics Muscles Physiology Muscles Research Methodology Muscles physiology Molekularbiologie Molekulargenetik Muskel Methode Muskelentwicklung Zellkultur |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007893137&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT emersoncharlesphillips methodsinmusclebiology |