Laser tweezers in cell biology:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
San Diego, Calif. [u.a.]
Acad. Press
1998
|
| Schriftenreihe: | Methods in cell biology
55 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 228 S. Ill., graph. Darst. |
| ISBN: | 0126393702 0125641575 |
Internformat
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| 245 | 1 | 0 | |a Laser tweezers in cell biology |c ed. by Michael P. Sheetz |
| 264 | 1 | |a San Diego, Calif. [u.a.] |b Acad. Press |c 1998 | |
| 300 | |a XII, 228 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in cell biology |v 55 | |
| 650 | 7 | |a Celbiologie |2 gtt | |
| 650 | 4 | |a Lasers en cytologie | |
| 650 | 7 | |a Lasertechniek |2 gtt | |
| 650 | 4 | |a Cytological Techniques | |
| 650 | 4 | |a Lasers | |
| 650 | 4 | |a Lasers in cytology | |
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Datensatz im Suchindex
| _version_ | 1804126242574696448 |
|---|---|
| adam_text | CONTENTS
Contributors ix
Preface xi
1. Forces of a Single Beam Gradient Laser Trap on a Dielectric Sphere in the
Ray Optics Regime
A. Ashkin
I. Introduction 1
II. Light Forces in the Ray Optics Regime 4
III. Force of the Gradient Trap on Spheres 9
IV. Effect of Mode Profiles and Index of Refraction on Trapping Forces 16
V. Concluding Remarks 20
Appendix I: Force of a Ray on a Dielectric Sphere 21
Appendix II: Force on a Sphere for Trap Focus along Y Axis 22
Appendix III: Force on a Sphere for an Arbitrarily Located Trap Focus 23
References 25
2. Basic Laser Tweezers
Ronald E. Sterba and Michael P. Sheetz
I. General Description 29
II. Microscope 30
III. Laser 33
IV. Optics and Layout 35
V. System Setup 36
VI. Alignment 37
VII. Translation 38
VIII. Video Recording and Analysis 38
IX. Accessories 39
X. Summary 40
Appendix: Laser Tweezers Parts List 40
References 41
3. A Simple Assay for Local Heating by Optical Tweezers
Scot C. Kuo
I. Introduction 43
II. Methods 44
III. Results 44
References 45
v
4. Reflections of a Lucid Dreamer: Optical Trap Design Considerations
Amit D. Mehta, Jeffrey T. Finer, and James A. Spudich
I. Introduction 47
II. Choice of Trapping Laser 48
III. Optical Layout 50
IV. Imaging 53
V. High Resolution Position Measurement 56
VI. Noise Sources 58
VII. Feedback 62
VIII. Calibration 65
IX. Analysis 67
X. Conclusion 68
References 68
5. Laser Scissors and Tweezers
Michael W. Berns, Yona Tadir, Hong Liang, and Bruce Trotnberg
I. Introduction 71
II. Mechanisms of Interaction 74
III. Biological Studies 81
IV. Summary 94
References 94
6. Optical Force Microscopy
Andrea L. Stout and Watt W. Webb
I. Introduction 99
II. AFM like Applications 100
III. Experimental Design 105
IV. New Directions 113
References 115
7. Single Molecule Imaging and Nanomanipulation of Biomolecules
Yoshie Harada, Takashi Funatsu, Makio Tokunaga, Khvamu Saito, Hideo Higuchi,
Yoshiharu Ishii, and Toshio Yanagida
I. Introduction 117
II. Visualization of Single Fluorophores in Aqueous Solution 118
III. Application 123
IV. Perspectives 127
References 128
8. Signals and Noise in Micromechanical Measurements
Frederick Gittes and Christoph F. Schmidt
I. Introduction 129
II. Spectral Data Analysis 131
III. Brownian Motion of a Harmonically Bound Particle 136
Contents vii
IV. Noise Limitations on Micromechanical Experiments 140
V. Sources of Instrumental Noise 147
VI. Conclusions 154
References 154
9. Cell Membrane Mechanics
Jianwu Dai and Michael P. Sheetz
I. Perspectives and Overview 157
II. Laser Optical Tweezers 160
III. Bead Coating 160
IV. Calibration of the Laser Tweezers 161
V. Tracking the Bead Position 165
VI. Membrane Tether Formation and Tether Force Measurement 166
VII. Membrane Tether Force and Membrane Mechanical Properties 167
VIII. Membrane Tension and Its Significance 168
References 170
10. Application of Laser Tweezers to Studies of the Fences and Tethers of
the Membrane Skeleton that Regulate the Movements of Plasma
Membrane Proteins
Akihiro Kusumi, Yasushi Sako, Takahiro Fujiwara, and Michio Tomishige
I. Introduction 174
II. Studying Various Modes of Protein Motion in the Plasma Membrane
Using Single Particle Tracking 176
III. Technical Details for SPT 176
IV. Instrumental Setup and Calibration of Laser Tweezers 179
V. Dragging Receptor Molecules along the Plasma Membrane and Finding
Obstacles in the Path 181
VI. Laser Tweezers Together with SPT Can Reveal the Presence of Elastic
Intercompartmental Barriers 185
VII. Studying the Fence and Tether Effects of the Membrane Skeleton Using
Laser Tweezers 188
VIII. SPT and Laser Tweezers Are Opening Up Possibilities for Scientists to
Learn about the Molecular Mechanics in Cells by Directly Handling Single
Molecules in Living Cells 192
References 192
11. In Vivo Manipulation of Internal Cell Organelles
Harald Feigner, Franz Grolig, Otto Muller, and Manfred Schliwa
I. Introduction 195
II. Optical Tweezers Setup 196
III. Reticulomyxa: Organelle Movement and Artificial Membrane Tubes 197
IV. Spirogyra: Cytoplasmic Streaming 199
References 202
Vlil Contents
12. Optical Chopsticks: Digital Synthesis of Multiple Optical Traps
Justin E. Molloy
I. Introduction 205
II. Trapping Configurations 206
III. Mechanical Deflectors (Mirrors) 208
IV. Acousto optic Deflectors 208
V. Position: Control and Noise 210
VI. Computer Control of Trap Positions 212
VII. Other Developments 213
References 215
Index 217
Volumes in Series 223
|
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| discipline | Physik Biologie |
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| indexdate | 2024-07-09T18:14:21Z |
| institution | BVB |
| isbn | 0126393702 0125641575 |
| language | English |
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| physical | XII, 228 S. Ill., graph. Darst. |
| publishDate | 1998 |
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| publisher | Acad. Press |
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| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Laser tweezers in cell biology ed. by Michael P. Sheetz San Diego, Calif. [u.a.] Acad. Press 1998 XII, 228 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 55 Celbiologie gtt Lasers en cytologie Lasertechniek gtt Cytological Techniques Lasers Lasers in cytology Laser (DE-588)4034610-9 gnd rswk-swf Cytologie (DE-588)4070177-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Cytologie (DE-588)4070177-3 s Laser (DE-588)4034610-9 s DE-604 Sheetz, Michael P. edt Methods in cell biology 55 (DE-604)BV002534878 55 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007893094&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Laser tweezers in cell biology Methods in cell biology Celbiologie gtt Lasers en cytologie Lasertechniek gtt Cytological Techniques Lasers Lasers in cytology Laser (DE-588)4034610-9 gnd Cytologie (DE-588)4070177-3 gnd |
| subject_GND | (DE-588)4034610-9 (DE-588)4070177-3 (DE-588)4143413-4 |
| title | Laser tweezers in cell biology |
| title_auth | Laser tweezers in cell biology |
| title_exact_search | Laser tweezers in cell biology |
| title_full | Laser tweezers in cell biology ed. by Michael P. Sheetz |
| title_fullStr | Laser tweezers in cell biology ed. by Michael P. Sheetz |
| title_full_unstemmed | Laser tweezers in cell biology ed. by Michael P. Sheetz |
| title_short | Laser tweezers in cell biology |
| title_sort | laser tweezers in cell biology |
| topic | Celbiologie gtt Lasers en cytologie Lasertechniek gtt Cytological Techniques Lasers Lasers in cytology Laser (DE-588)4034610-9 gnd Cytologie (DE-588)4070177-3 gnd |
| topic_facet | Celbiologie Lasers en cytologie Lasertechniek Cytological Techniques Lasers Lasers in cytology Laser Cytologie Aufsatzsammlung |
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