Polyamine protocols:
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
Totowa, NJ
Humana Press
1998
|
Schriftenreihe: | Methods in molecular biology
79 |
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | IX, 186 S. graph. Darst. |
ISBN: | 0896034488 |
Internformat
MARC
LEADER | 00000nam a2200000 cb4500 | ||
---|---|---|---|
001 | BV011644011 | ||
003 | DE-604 | ||
005 | 00000000000000.0 | ||
007 | t | ||
008 | 971124s1998 d||| |||| 00||| eng d | ||
020 | |a 0896034488 |9 0-89603-448-8 | ||
035 | |a (OCoLC)37553743 | ||
035 | |a (DE-599)BVBBV011644011 | ||
040 | |a DE-604 |b ger |e rakddb | ||
041 | 0 | |a eng | |
049 | |a DE-12 | ||
050 | 0 | |a QP801.P638 | |
082 | 0 | |a 572/.548 |2 21 | |
084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
245 | 1 | 0 | |a Polyamine protocols |c ed. by David M. L. Morgan |
264 | 1 | |a Totowa, NJ |b Humana Press |c 1998 | |
300 | |a IX, 186 S. |b graph. Darst. | ||
336 | |b txt |2 rdacontent | ||
337 | |b n |2 rdamedia | ||
338 | |b nc |2 rdacarrier | ||
490 | 1 | |a Methods in molecular biology |v 79 | |
650 | 7 | |a Polyaminen |2 gtt | |
650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
650 | 4 | |a Wissenschaftliches Arbeiten | |
650 | 4 | |a Polyamines |v Laboratory manuals | |
650 | 4 | |a Polyamines |x analysis | |
650 | 4 | |a Polyamines |x metabolism | |
650 | 0 | 7 | |a Polyamine |0 (DE-588)4175101-2 |2 gnd |9 rswk-swf |
650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
650 | 0 | 7 | |a Molekularbiologie |0 (DE-588)4039983-7 |2 gnd |9 rswk-swf |
689 | 0 | 0 | |a Polyamine |0 (DE-588)4175101-2 |D s |
689 | 0 | 1 | |a Molekularbiologie |0 (DE-588)4039983-7 |D s |
689 | 0 | 2 | |a Methode |0 (DE-588)4038971-6 |D s |
689 | 0 | |5 DE-604 | |
700 | 1 | |a Morgan, David M. L. |e Sonstige |4 oth | |
830 | 0 | |a Methods in molecular biology |v 79 |w (DE-604)BV035362695 |9 79 | |
856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007847850&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
999 | |a oai:aleph.bib-bvb.de:BVB01-007847850 |
Datensatz im Suchindex
_version_ | 1804126177319714816 |
---|---|
adam_text | Contents
Preface v
Contributors ix
Part I. Introduction 1
1 Polyamines: An Introduction
David M. L. Morgan 3
Part II. Assay Methods for Enzymes of Polyamine Biosynthesis 31
2 Determination of Ornithine Decarboxylase Activity Using [3H]Ornithine
AmaliaTabib 33
3 Assay of Mammalian Ornithine Decarboxylase Activity Using
[uC]Ornithine
Catherines. Coleman and Anthony E. Pegg 41
4 Assay of Mammalian S Adenosylmethionine Decarboxylase Activity
LisaM. Shantz and Anthony E. Pegg 45
5 Assay of Spermidine and Spermine Synthases
Laurie Wiest and Anthony E. Pegg 51
6 Measurement of Spermidine/Spermine /V Acetyltransferase Activity
Heather M. Wallace and Deborah M. Evans 59
7 Assay of Spermidine /V Acetyltransferase
Jim Blankenship 69
Part III. Assay Methods for Enzymes of Polyamine Catabolism 75
8 Assay of AP Acetylspermidine Deacetylase
Jim Blankenship 77
9 Hydrogen Peroxide Assay for Amine Oxidase Activity
R. James Storer and Antonio Ferrante 81
10 Radiochemical Assay of Diamine Oxidase
R. James Storer and Antonio Ferrante 91
11 Radiochemical Estimation of Polyamine Oxidase
David M. L. Morgan 97
vii
viii Contents
12 Measurement of Aldehyde Formation as an Assay for Polyamine
Oxidase Activity
David M. L. Morgan 105
Part IV. Measurement of Polyamines 109
13 Determination of Polyamines as Their Benzoylated Derivatives
by HPLC
David M. L Morgan /11
14 A Dansyl Chloride HPLC Method for the Determination of Polyamines
Karl J. Hunter 119
15 The Determination of Polyamines and Amino Acids
by a Fluorescamine HPLC Method
KarlJ. Hunter and Alan H. Fairlamb 125
16 Thin Layer Chromatographic Method for Assaying Polyamines
Rentala Madhubala 131
Part V. Measurement of Polyamine Transport 137
17 Measurement of Polyamine Transport: Adherent Cells
David M. L. Morgan 139
18 Measurement of Polyamine Transport: Cells in Suspension
Sarah A. Le Quesne and Alan H. Fairlamb 149
19 Measurement of Polyamine Efflux from Cells in Culture
Heather M. Wallace and A. Jill Mackarel 157
Part VI. Measurement of Polyamine Effects on Cell Growth 167
20 Leucine Incorporation and Thymidine Incorporation
Christopher P. Denton 169
21 Tetrazolium (MTT) Assay for Cellular Viability and Activity
David M. L. Morgan 179
Index 185
|
any_adam_object | 1 |
building | Verbundindex |
bvnumber | BV011644011 |
callnumber-first | Q - Science |
callnumber-label | QP801 |
callnumber-raw | QP801.P638 |
callnumber-search | QP801.P638 |
callnumber-sort | QP 3801 P638 |
callnumber-subject | QP - Physiology |
classification_rvk | WC 4150 |
ctrlnum | (OCoLC)37553743 (DE-599)BVBBV011644011 |
dewey-full | 572/.548 |
dewey-hundreds | 500 - Natural sciences and mathematics |
dewey-ones | 572 - Biochemistry |
dewey-raw | 572/.548 |
dewey-search | 572/.548 |
dewey-sort | 3572 3548 |
dewey-tens | 570 - Biology |
discipline | Biologie |
format | Book |
fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01781nam a2200481 cb4500</leader><controlfield tag="001">BV011644011</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">971124s1998 d||| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0896034488</subfield><subfield code="9">0-89603-448-8</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)37553743</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV011644011</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-12</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QP801.P638</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572/.548</subfield><subfield code="2">21</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Polyamine protocols</subfield><subfield code="c">ed. by David M. L. Morgan</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">1998</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">IX, 186 S.</subfield><subfield code="b">graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in molecular biology</subfield><subfield code="v">79</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Polyaminen</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Wetenschappelijke technieken</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Wissenschaftliches Arbeiten</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Polyamines</subfield><subfield code="v">Laboratory manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Polyamines</subfield><subfield code="x">analysis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Polyamines</subfield><subfield code="x">metabolism</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Polyamine</subfield><subfield code="0">(DE-588)4175101-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Molekularbiologie</subfield><subfield code="0">(DE-588)4039983-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Polyamine</subfield><subfield code="0">(DE-588)4175101-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Molekularbiologie</subfield><subfield code="0">(DE-588)4039983-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Morgan, David M. L.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in molecular biology</subfield><subfield code="v">79</subfield><subfield code="w">(DE-604)BV035362695</subfield><subfield code="9">79</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007847850&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-007847850</subfield></datafield></record></collection> |
id | DE-604.BV011644011 |
illustrated | Illustrated |
indexdate | 2024-07-09T18:13:18Z |
institution | BVB |
isbn | 0896034488 |
language | English |
oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007847850 |
oclc_num | 37553743 |
open_access_boolean | |
owner | DE-12 |
owner_facet | DE-12 |
physical | IX, 186 S. graph. Darst. |
publishDate | 1998 |
publishDateSearch | 1998 |
publishDateSort | 1998 |
publisher | Humana Press |
record_format | marc |
series | Methods in molecular biology |
series2 | Methods in molecular biology |
spelling | Polyamine protocols ed. by David M. L. Morgan Totowa, NJ Humana Press 1998 IX, 186 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 79 Polyaminen gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Polyamines Laboratory manuals Polyamines analysis Polyamines metabolism Polyamine (DE-588)4175101-2 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Polyamine (DE-588)4175101-2 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Morgan, David M. L. Sonstige oth Methods in molecular biology 79 (DE-604)BV035362695 79 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007847850&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Polyamine protocols Methods in molecular biology Polyaminen gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Polyamines Laboratory manuals Polyamines analysis Polyamines metabolism Polyamine (DE-588)4175101-2 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
subject_GND | (DE-588)4175101-2 (DE-588)4038971-6 (DE-588)4039983-7 |
title | Polyamine protocols |
title_auth | Polyamine protocols |
title_exact_search | Polyamine protocols |
title_full | Polyamine protocols ed. by David M. L. Morgan |
title_fullStr | Polyamine protocols ed. by David M. L. Morgan |
title_full_unstemmed | Polyamine protocols ed. by David M. L. Morgan |
title_short | Polyamine protocols |
title_sort | polyamine protocols |
topic | Polyaminen gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Polyamines Laboratory manuals Polyamines analysis Polyamines metabolism Polyamine (DE-588)4175101-2 gnd Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
topic_facet | Polyaminen Wetenschappelijke technieken Wissenschaftliches Arbeiten Polyamines Laboratory manuals Polyamines analysis Polyamines metabolism Polyamine Methode Molekularbiologie |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007847850&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
volume_link | (DE-604)BV035362695 |
work_keys_str_mv | AT morgandavidml polyamineprotocols |