Immunochemistry in practice:
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | Undetermined |
| Veröffentlicht: |
Oxford [u.a.]
Blackwell Science
1996
|
| Ausgabe: | 3. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 362 S. Ill., graph. Darst. |
| ISBN: | 0865426333 |
Internformat
MARC
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| 100 | 1 | |a Johnstone, Alan |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Immunochemistry in practice |c Alan Johnstone ; Robin Thorpe |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a Oxford [u.a.] |b Blackwell Science |c 1996 | |
| 300 | |a XV, 362 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 650 | 4 | |a Immunchemie | |
| 650 | 4 | |a Immunochemistry |x Technique | |
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Datensatz im Suchindex
| _version_ | 1804126142329782272 |
|---|---|
| adam_text | FOREWORD, XI PREFACE, XII ACKNOWLEDGEMENTS, XIV 1 BASIC TECHNIQUES, 1
1.1 DETECTION AND MEASUREMENT OF PROTEINS, 1 1.1.1 ULTRAVIOLET
ABSORPTION, 1 1.1.2 FOLIN PHENOL METHOD, 3 1.1.3 DYE-BINDING ASSAYS, 4
1.2 PROTEIN FRACTIONATION, 6 1.2.1 GEL FILTRATION. 6 1.2.2 ION EXCHANGE
CHROMATOGRAPHY, 11 1.2.3 HYDROXYLAPATITE, 18 1.2.4 HPLC, 18 1.3 DIALYSIS
AND CONCENTRATION OF PROTEINS, 19 1.3.1 DIALYSIS, 19 1.3.2
CONCENTRATION, 22 1.4 MEASUREMENT OF RADIOACTIVITY, 23 1.5
AUTORADIOGRAPHY, 27 1 S.1 DIRECT AUTORADIOGRAPHY, 28 1.5.2 FLUOROGRAPHY,
29 1.5.3 PRE-EXPOSURE OF FILM, 3 1 1.5.4 RADIOACTIVE MARLTER PEN, 32
PRODUCTION OF ANTIBODIES, 34 2.1 INTRODUCTION, 34 2.2 PRODUCTION OF
POLYCLONAL ANTISERA. 35 2.2.1 IMMUNIZATION PROTOCOLS, 36 2.2.2 BLEEDING,
38 2.2.3 ISOLATION OF SERURN FROM BLOOD, 39 2.2.4 PREPARATION OF SERUM
FROM PLASMA, 40 2.3 MONOCLONAL ANTIBODIES, 40 2.3.1 CE11 FUSION AND
SELECTION OF ANTIBODY-SECRETING HYBRIDOMAS, 41 2.3.2 CLONING HYBRIDOMAS,
45 2.3.3 PRODUCTION OF ASCITIC FLUID, 47 2.3.4 PRODUCTION OF MONOCLONAL
ANTIBODIES IN VITRO, 48 CONTENTS 2.3.5 DETERMINATION OF CLASS AND
SUBCLASS OF MONOCLONAL ANTIBODIES, 49 2.3.6 VIRAL TRANSFORMATION OF
HUMAN B LYMPHOCYTES FOR THE PRODUCTION OF MONOCLONAL ANTIBODIES, 5 1 2.4
USE OF PEPTIDES FOR PRODUCTION OF ANTIBODIES, 54 2.4.1 CHOICE OF CARRIER
PROTEIN, 54 2.4.2 CHOICE OF PEPTIDE, 55 2.4.3 CONJUGATION PROCEDURES, 56
2.4.4 IMMUNIZATION WITH PEPTIDE-CARRIER PROTEIN CONJUGATES, 58 2.4.5
SCREENING ANTI-PEPTIDE ANTIBODIES, 58 3 PURIFICATION OF IMMUNOGLOBULINS,
CONSTITUENT CHAINS AND FRAGMENTS, 60 3.1 INTRODUCTION, 60 3.2
MANIPULATION AND STORAGE OF IMMUNOGLOBULINS, 61 3.3 ISOLATION OF IGG, 62
3.3.1 ISOLATION OF IGG FROM SERUM, 63 3.3.2 ISOLATION OF IGG FROM
CHICKEN EGGS, 66 3.4 PREPARATION OF FRAGRNENTS OF IGG, 68 3.4.1
SEPARATION OF HEAVY AND LIGHT CHAINS, 68 3.4.2 PREPARATION OF FAB AND FC
FRAGMENTS. 72 3.4.3 PREPARATION OF F (ABF)* FAB AND PFC FRAGMENTS. 74
3.4.4 PREPARATION OF FD AND V, FRAGMENTS, 76 3.4.5 FRAGMENTATION OF
MOUSE AND RAT MONOCLONAL IGG, 76 3.5 ISOLATION OF IGM, 79 3.6
PREPARATION OF FRAGMENTS OF IGM, 83 , 3.6.1 SEPARATION OF HEAVY, LIGHT
AND J CFFAINS, 83 3.6.2 ISOLATION OF J CHAIN FROM IGM: PREPARATION OF
MONOMER IGM, 83 3.6,3 PREPARATION OF FC* F(ABF)* AND FAB, FRAGMENTS, 85
3.6.4 FRAGMENTATION OF MOUSE AND RAT MONOCLONAL IGM, 87 3.7 ISOLATION OF
IGA, 88 3.7.1 ISOLATION OF IGA FROM SEMM, 88 3.7.2 ISOLATION OF
SECRETORY IGA FROM MILK AND COLOSTMM, 91 3.8 PREPARATION OF FRAGMENTS OF
IGA, 92 3.8.1 SEPARATION OF HEAVY, LIGHT AND J CHAINS, 93 3.8.2
ISOLATION OF J CHAIN FROM IGA, 95 3.8.3 ISOLATION OF SECRETORY
COMPONENT, 95 3.8.4 PREPARTION OF F(ABF)*, FAB , AND FC, FRAGMENTS, 96
3.9 ISOLATION OF IGE, 97 3.10 PREPARATION OF FRAGMENTS OF IGE, 99 3.11
ISOLATION OF IGD, 99 3.12 PREPARATION OF FRAGMENTS OF IGD, 100 4
ISOLATION AND FRACTIONATION OF LYMPHOCYTES, 101 4.1 MANIPULATION AND
STORAGE OF LYMPHOCYTES, 101 4.1.1 COUNTING CEIIS AND DETERMINING THEIR
VIABILITY, 105 4.1.2 GLUTARALDEHYDE HATION OF CELLS. 108 4.1.3 REMOVAL
OF SURFACE PROTEIN AND ITS REGENERATION, 109 CONTENTS 4.2 ISOLATION OF
LYMPHOCYTES, 110 4.2.1 ISOLATION OF LYMPHOCYTES FROM BLOOD, 110 4.2.2
SEPARATION OF LYMPHOCYTES, ERYTHROCYTES, PLATELETS AND PLASMA, 112 4.2.3
ISOLATION OF LYMPHOCYTES FROM SOLID TISSUE, 113 4.2.4 LYSIS OF
ERYTHROCYTES, 116 4.3 FRACTIONATION OF LYMPHOCYTES, 11 7 4.3.1
ENRICHMENT OF T CELLS, 117 4.3.2 ENRICHMENT OF B CEILS, 118 4.3.3
SPECIFIC ROSETTING TECHNIQUES, 120 4.3.4 OTHER FRACTIONATION TECHNIQUES,
121 4.4 SOLUBIIZATION OF CEILS AND MEMBRANES, 121 4.4.1 CHOICE OF
DETERGENT, 122 4.4.2 SOLUBILIZATION OF WHOLE NUCLEATED CELLS, 127 5
RADIOLABELLING TECHNIQUES, 129 5.1 INTRODUCTION, 129 5.1.1 RADIATION
SAFETY, 129 5.1.2 MEASUREMENT OF RADIOACTIVITY INCORPORATED INTO
PROTEIN, 13 1 5.2 RADIOIODINATION, 132 5.2.1 CHLORAMINE T IODINATION,
134 5.2.2 IODO-GEN IODINATION, 138 5.2.3 LACTOPEROXIDASE-CATALYSED
IODINATION, 13 9 5.2.4 OTHER IODIIATION TECHNIQUES, 141 5.3
RADIOLABELLING GLYCOPROTEINS AND GLYCOLIPIDS. 142 5.3.1 PERIODATE
OXIDATION, 143 5.3.2 GALACTOSE OXIDASE OXIDATION, 145 5.4 BIOSYNTHETIC
LABELIING, 146 5.4.1 BIOSYNTHETIC LABE L LING OF PROTEINS, 147 5.4.2
BIOSYNTHETIC LABELLING OF PHOSPHOPROTEINS AND PHOSPHOLIPIDS, 148 6
PRECIPITATION TECHNIQUES IN AGAR AND AGAROSE, 150 6.1 INTRODUCTION, 150
6.1.1 AFFINITY AND AVIDITY, 150 6.2 AGAR AND AGAROSE GEL TECHNIQUES:
BASIC CONSIDERATIONS, 150 6.2.1 PRECOATING GLASS PLATES WITH AGAR, 151
6.2.2 STAINING PRECIPITIN LINES, 152 6.3 DIFFUSION TECHNIQUES, 152 6.3.1
DOUBLE IMMUNOD8USION, 152 6.3.2 SINGLE (RADIAL) IMMUNOD8USION, 15 5
6.3.3 MODIFICATIONS FOR INSOLUBLE PROTEINS, 157 6.4 ELECTROPHORETIC
TECHNIQUES, 157 6.4.1 ELECTROENDOSMOSIS, 158 6.4.2
IMMUNOELECTROPHORESIS, 159 6.4.3 ROCKET IMMUNOELECTROPHORESIS, 162 6.4.4
CROSSED IRNMUNOELECTROPHORESIS, 164 7 ANALYTICAL ELECTROPHORESIS AND
ISOELECTRIC FOCUSING, 1 67 7.1 PREPARATION AND MANIPULATION OF
POLYACRYLAMIDE GELS, 167 VII CONTENTS 7.1.1 STAINING FOR PROTEIN, 168
7.1.2 STAINING FOR CARBOHYDRATE, 173 7.1.3 STORING AND DRYING
POLYACRYLAMIDE GELS, 174 7.1.4 SLICING POLYACRYLAMIDE GELS FOR
DETERMINING THEIR RADIOACTIVE PROFILE, 176 7.1.5 CONCENTRATION OF
SAMPLES FOR PAGE, 177 7.2 PAGE: GENERAL PRINCIPLES, 178 7.2.1 APPARATUS
FOR PAGE, 179 7.3 PAGE UNDER DENATURING CONDITIONS, 179 7.3.1 SDS-PAGE,
182 7.3.2 SDS-POLYACRYLAMIDE GRADIENT GEL ELECTROPHORESIS, 188 7.3.3
UREA PAGE, 191 7.3.4 DISC PAGE UNDER DENATURING CONDITIONS, 192 7.4 PAGE
UNDER NON-DENATURING CONDITIONS, 193 7.5 ISOELECTRIC FOCUSING IN
POLYACRYLAMIDE GEL, 193 7.5.1 INTRODUCTION, 193 7.5.2 FOCUSING IN
POLYACRYLAMIDE SLAB GELS, 194 7.5.3 ISOELECTRIC FOCUSING IN THE PRESENCE
OF UREA OR DETERGENTS, 198 7.5.4 ISOELECTRIC FOCUSING IN AGAROSE GEL,
198 7.6 TWO-DIMENSIONAL ISOELECTRIC FOCUSING AND SDS-PAGE, 201 7.7
PEPTIDE MAPPING USING PROTEOLYTIC ENZYMES AND SDS-PAGE, 205 8 SPECIFIC
DETECTION OF ANTIGENS SEPARATED BY POLYACRYLAMIDE GEL ELECTROPHORESIS,
208 8.1 INTRODUCTION, 208 8.2 CROSSED IMMUNOELECTROPHORESIS USING PAGE
IN THE FIRST DIMENSION, 209 8.3 DIRECT APPLICATION OF ANTIBODIES TO
POLYACRYLAMIDE GELS, 211 8.4 TRANSFER OF PROTEINS FROM GELS TO POROUS
MEMBRANES (IMMUNOBLOTTING), 21 1 8.4.1 PREPARATION OF DIAZOTIZED PAPER,
212 8.4.2 TRANSFER BY DIIUSION, 214 8.4.3 ELECTROPHORETIC TRANSFER, 214
8.4.4 TRANSFER TO NTROCELLULOSE SHEETS AND RADIOLABELLED
IMRNUNOBLOTTING, 21 7 8.4.5 ECL DETECTION, 220 8.4.6 TRANSFER TO
DIAZOTIZED PAPER, 225 8.4.7 REMOVAL OF ANTIBODY FROM DIAZOTIZED PAPER
BLOTS, 225 8.5 FILTER &ITY TRANSFER (FAT), 226 8.5.1 DIRECT FAT, 226
8.5.2 FAT SANDWICH, 228 8.6 MODIICATIONS, 228 9 PREPARATIVE GEL
TECHNIQUES, 230 9.1 ISOLATION OF PRECIPITATED ANTIBODY-ANTIGEN
CORNPLEXES, 230 9.1.1 PREPARATIVE DOUBLE DIIUSION. 230 9.1.2
IMMUNIZATION WITH ISOLATED PRECIPITATES, 231 CONTENTS 9.1.3 ANALYSIS OF
PRECIPITATES BY SDS-PAGE, 231 9.2 ISOLATION OF PURIFIED PROTEINS, 212
9.2.1 PREPARATIVE SDS-PAGE, 232 9.2.2 PREPARATIVE PAGE BLOTTING, 235
9.2.3 PREPARATIVE ISOELECTRIC FOCUSING, 237 9.2.4 BLOCK ELECTROPHORESIS,
239 10 AFFINITY CHROMATOGRAPHY AND IRNMUNOPRECIPITATION, 241 10.1
INTRODUCTION, 241 10.2 THE INSOLUBLE DERIVATIVE, 243 10.2.1 CNBR
COUPLING TO AGAROSE BEADS. 244 10.2.2 PREPARATION OF STAPHYLOCOCCUS
AUREUS ADSORBENT, 249 10.3 AFFINITY CHROMATOGRAPHY FRACTIONATION, 252
10.3.1 IMMUNOADSORPTION AND PURIFICATION OF ANTIBODIES, 254 10.3.2
FRACTIONATION OF IGG INTO SUBCLASSES: ISOLATION OF MOUSE IGG
MONOCLONALS, 256 10.3.3 INSOLUBILIZCD ANTIBODIES, 259 10.3.4
INSOLUBILIZED LECTINS, 260 10.3.5 AFFINITY CHRORNATOGRAPHY OF CELLS. 266
10.4 IRNMUNOPRECIPITATION, 268 10.4.1 IMMUNOPRECIPITATION USING PROTEIN
A, 270 10.4.2 LMMUNOPRECIPITATION USING ANTIBODIES WHICH DO NOT BIND
PROTEIN A, 272 11 COUPLING ANTIBODIES TO FLUOROCHROMES, ENZYMES AND
BIOTIN, 277 11.1 INTRODUCTION, 277 11.2 CONJUGATION OF FLUOROCHROMES TO
IRNMUNOGLOBULINS, 278 11.2.1 CONJUGATION WITH FLUORESCEIN OR
TETRAMETHYLRHODAMINE ISOTHIOCYANATE, 278 11.2.2 CONJUGATION TO
PHYCOERYTHRIN, 280 11.3 CONJUGATION WITH ENZYMES, 281 1 1.3.1
CONJUGATION USING GLUTARALDEHYDE, 28 1 11.3.2 CONJUGATION USING
PERIODATE, 282 11.3.3 CONJUGATION USING MALEIMIDE REAGENTS, 284 11.4
CONJUGATION WITH BIOTIN, 288 12 IMMUNOASSAYS, 290 12.1 SOLID-PHASE
BINDING IMRNUNOASSAYS FOR ANTIBODIES, 292 12.1.1 ANTIBODIES DIRECTED
AGAINST SOLUBLE ANTIGENS, 293 12.1.2 ANTIBODIES DIRECTED AGAINST CELL
SURFACE ANTIGENS, 297 12.2 COMPETITIVE BINDING IMRNUNOASSAYS, 298 12.2.1
SETTING UP A COMPETITIVE IMMUNOASSAY, 299 12.2.2 FARR-TYPE ASSAY, 303
12.3 IMMUNOMETRIC ASSAYS (IRMA AND TWO-SITE ELISA), 306 12.4 IMMUNOASSAY
VALIDATION, SENSITIVITY, STANDARDIZATION AND OTHER PROBLEMS, 3 11
CONTENTS 3 LMMUNOCYTOCHEMISTRY, IM UNOHISTOCHEMISTRY AND FLOW
CYTOFLUORIMETRY, 31 3 13 .L LNTRODUCTION, 31 3 13.2 IMMUNOFLUORESCENCE
TECHNIQUES, 314 13.2.1 FLUORESCENCE MICROSCOPES, 314 13.2.2 FLOW
CYTOFLUORIMETRY, 3 15 13 .2.3 LABELLING OF WHOLE CELLS IN SUSPENSION,
317 13.2.4 CYTOFLUORIMETNC ANALYSIS OF HYBRIDOMA SUPERNATANTS FOR
ANTIBODIES AGAINST CELL SURFACE MOLECULES, 320 13.2.5 LABELLING OF FIXED
CELLS FOR IMMUNOFLUORESCENCE MICROSCOPY, 322 13.2.6 LABELIING TISSUE
SECTIONS FOR IMMUNOFLUORESCENCE RNICROSCOPY, 326 13.3 ENZYME LABE1
TECHNIQUES FOR IMMUNOHISTOCHEMISTRY AND IMMUNOCYTOCHEMISTRY, 330 13 $3.1
LABELLING USING PEROXIDASE-CONJUGATED ANTIBODIES, 330 13.3.2
PEROXIDASE-ANTI-PEROXIDASE (PM) LABELLING, 332 13.3.3 LABELLING WITH
ALKALINE PHOSPHATASE-CONJUGATED ANTIBODIES, 3 36 13.3.4 ALKALINE
PHOSPHATASE-ANTI-ALKALINE PHOSPHATASE (APAAP) LABEILING, 3 3 7 13.4
ELECTRON MICROSCOPY, 33 8 APPENDIX 1 : ABBREVIATIONS, 339 APPENDIX 2:
BUFFERS, 341 APPENDIX 3: MANUFACTURERS AND SUPPLIERS, 343 REFERENCES,
347 INDEX, 355 COLOUR PLATE SECTION OPPOSITE P. 320
|
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| dewey-ones | 571 - Physiology & related subjects |
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| discipline | Biologie Chemie Medizin |
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| id | DE-604.BV011613639 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:12:45Z |
| institution | BVB |
| isbn | 0865426333 |
| language | Undetermined |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007823151 |
| oclc_num | 247118047 |
| open_access_boolean | |
| owner | DE-20 DE-91G DE-BY-TUM |
| owner_facet | DE-20 DE-91G DE-BY-TUM |
| physical | XV, 362 S. Ill., graph. Darst. |
| publishDate | 1996 |
| publishDateSearch | 1996 |
| publishDateSort | 1996 |
| publisher | Blackwell Science |
| record_format | marc |
| spelling | Johnstone, Alan Verfasser aut Immunochemistry in practice Alan Johnstone ; Robin Thorpe 3. ed. Oxford [u.a.] Blackwell Science 1996 XV, 362 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Immunchemie Immunochemistry Technique Immunchemie (DE-588)4161366-1 gnd rswk-swf Immunchemie (DE-588)4161366-1 s DE-604 Thorpe, Robin Verfasser aut OEBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007823151&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Johnstone, Alan Thorpe, Robin Immunochemistry in practice Immunchemie Immunochemistry Technique Immunchemie (DE-588)4161366-1 gnd |
| subject_GND | (DE-588)4161366-1 |
| title | Immunochemistry in practice |
| title_auth | Immunochemistry in practice |
| title_exact_search | Immunochemistry in practice |
| title_full | Immunochemistry in practice Alan Johnstone ; Robin Thorpe |
| title_fullStr | Immunochemistry in practice Alan Johnstone ; Robin Thorpe |
| title_full_unstemmed | Immunochemistry in practice Alan Johnstone ; Robin Thorpe |
| title_short | Immunochemistry in practice |
| title_sort | immunochemistry in practice |
| topic | Immunchemie Immunochemistry Technique Immunchemie (DE-588)4161366-1 gnd |
| topic_facet | Immunchemie Immunochemistry Technique |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007823151&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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