Studies on arabidopsis thaliana cells in vitro: somatic embryogenesis and protoplast culture
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
1997
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VI, 105 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV011609240 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 971104s1997 ad|| m||| 00||| engod | ||
| 016 | 7 | |a 952676818 |2 DE-101 | |
| 035 | |a (OCoLC)258062822 | ||
| 035 | |a (DE-599)BVBBV011609240 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-19 |a DE-29T |a DE-12 |a DE-355 |a DE-11 |a DE-188 | ||
| 100 | 1 | |a Luo, Yingkun |d 1964- |e Verfasser |0 (DE-588)118083651 |4 aut | |
| 245 | 1 | 0 | |a Studies on arabidopsis thaliana cells in vitro |b somatic embryogenesis and protoplast culture |c von Yingkun Luo |
| 264 | 1 | |c 1997 | |
| 300 | |a VI, 105 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 1997 | ||
| 650 | 0 | 7 | |a Zellkultur |0 (DE-588)4067547-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Somatische Embryogenese |0 (DE-588)4319974-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Ackerschmalwand |0 (DE-588)4141299-0 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Ackerschmalwand |0 (DE-588)4141299-0 |D s |
| 689 | 0 | 1 | |a Zellkultur |0 (DE-588)4067547-6 |D s |
| 689 | 0 | 2 | |a Somatische Embryogenese |0 (DE-588)4319974-4 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007819422&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-007819422 | |
Datensatz im Suchindex
| _version_ | 1807681794373320704 |
|---|---|
| adam_text |
CONTENTS
III
CONTENTS
SUMMARY
1
INTRODUCTION
1.
GENERAL
DESCRIPTION
OF
SOMATIC
EMBRYOGENESIS
2
2.
ARABIDOPSIS
-
A
MODEL
SYSTEM
FOR
PLANT
MOLECULAR
AND
DEVELOPMENTAL
STUDIES
7
3.
EMBRYOGENESIS
IN
ARABIDOPSIS
THALIANA
7
4.
REGENERATION
FROM
PROTOPLAST
CULTURES
OF
ARABIDOPSIS
THALIANA
10
5.
RESEARCH
AIMS
11
MATERIALS
AND
METHODS
1.
CHEMICALS
13
2.
DESCRIPTION
OF
PLANT
LINES
14
2.1.
ECOTYPES
14
2.2.
MUTANTS
15
2.3.
MOLECULAR
MARKER
LINE
16
3.
GROWTH
OF
DONOR
PLANTS
16
3.1.
PLANTS
FOR
IMMATURE
ZYGOTIC
EMBRYO
CULTURE
AND
CROSSES
16
3.2.
PLANTS
FOR
PROTOPLAST
CULTURE
16
4.
ISOLATION
AND
CULTURE
OF
ZYGOTIC
EMBRYOS
17
4.1.
ISOLATION
OF
IMMATURE
ZYGOTIC
EMBRYOS
17
4.2.
IMMATURE
ZYGOTIC
EMBRYO
CULTURE
18
4.3.
DETERMINATION
OF
THE
EFFICIENCY
OF
SOMATIC
EMBRYOGENESIS
19
4.4.
GERMINATION
OF
SOMATIC
EMBRYOS
20
5.
PROTOPLAST
ISOLATION,
EMBEDDING
AND
CULTURE
20
5.1.
PROTOPLAST
ISOLATION
20
5.1.1.
ENZYME
DIGESTION
20
5.1.2.
PROTOPLAST
ISOLATION
21
CONTENTS
IV
METHOD
A
21
METHOD
B
22
5.2.
PROTOPLAST
EMBEDDING
24
5.2.1.
POLYPROPYLENE
EMBEDDING
SYSTEM
24
ALGINATE
FILM
CULTURES
24
ALGINATE
LAYER
CULTURES
25
5.2.2.
METAL
MESH
EMBEDDING
SYSTEM
25
5.3.
PROTOPLAST
CULTURE
26
5.3.1.
ALGINATE
FILM
CULTURE
26
5.3.2.
ALGINATE
LAYER
CULTURE
27
5.3.3.
REGENERATION
27
6.
CROSSES
28
7.
HISTOLOGICAL
SECTIONS
28
7.1.
FIXATION
AND
EMBEDDING
28
7.2.
SECTIONING
29
7.3.
STAINING
AND
MICROSCOPY
29
8.
HISTOCHEMICAL
LOCALIZATION
OF
GUS
ACTIVITY
30
9.
CYTOLOGICAL
TECHNIQUES
30
9.1.
WHOLE
MOUNT
PREPARATIONS
30
9.2.
DAPI
STAINING
31
10.
IMAGE
PROCESSING
32
RESULTS
PART
I:
INDUCTION
OF
SOMATIC
EMBRYOGENESIS
FROM
ZYGOTIC
EMBRYOS
EXPLANTED
INTO
LIQUID
MEDIUM
AND
LEAF
PROTOPLASTS
34
1.
FACTORS
INFLUENCING
SOMATIC
EMBRYOGENESIS
FROM
WILD-TYPE
TISSUES
34
1.1
DEVELOPMENTAL
STAGE
OF
THE
EXPLANT
34
1.1.1.
STAGING
OF
ZYGOTIC
EMBRYOS
34
1.1.2.
THE
INFLUENCE
OF
DEVELOPMENTAL
STAGES
ON
SOMATIC
EMBRYOGENESIS
35
1.2.
ECOTYPE
37
CONTENTS
V
1.3.
PHYTOHORMONE
COMPOSITIONS
40
1.3.1.
AUXINS
40
1.3.2.
CYTOKININS
42
1.4.
OTHER
FACTORS
43
2.
ANALYSIS
OF
THE
RESPONSE
FROM
PARTIAL
EMBRYOS:
SITES
AND
TISSUES
OF
EMBRYO
FORMATION
43
3.
DISSECTION
OF
CULTURED
ZYGOTIC
EMBRYOS:
THE
EARLY
STAGES
OF
SOMATIC
EMBRYOGENESIS
44
4.
EXPRESSION
OF
A
FUSION
GENE
BETWEEN
THE
ATLTPL
PROMOTER
AND
THE
GUS
REPORTER
IN
ZYGOTIC
AND
SOMATIC
EMBRYOS
47
5.
MORPHOLOGY
AND
INTERNAL
STRUCTURE
OF
WILD-TYPE
SOMATIC
EMBRYOS
48
6.
MATURATION
AND
GERMINATION
OF
WILD-TYPE
SOMATIC
EMBRYOS
49
7.
GENETIC
ANALYSIS
OF
SOMATIC
EMBRYOGENESIS
53
8.
SOMATIC
EMBRYOGENESIS
IN
PATTERN
FORMATION
MUTANTS
OF
A.
THALIANA
54
S.L.GNOM
54
8.2.
MONOPTEROS
55
8.3.
GURKE
AND
FACKEL
56
9.
EARLY
SOMATIC
EMBRYOGENESIS
FROM
LEAF
PROTOPLASTS
58
PART
II:
RAPID
AND
EFFICIENT
REGENERATION
OF
PLANTS
FROM
PROTOPLASTS
OF
A.
THALIANA
60
10.
FACTORS
INFLUENCING
THE
PHYSIOLOGICAL
STATES
OF
THE
DONOR
PLANTS
60
10.1.
CULTURE
VESSEL
60
10.2.
PHOTOPERIOD
61
10.3.
MEDIUM
AND
VOLUME
61
10.4.
NUMBER
OF
PLANTS
IN
THE
CULTURE
VESSEL
62
11.
PROTOPLAST
ISOLATION
62
11.1.
INCUBATION
MEDIUM
62
11.2.
VACUUM
INFILTRATION
63
11.3.
METHOD
OF
PROTOPLAST
ISOLATION
63
12.
PROTOPLAST
EMBEDDING
63
12.1.
GELLING
AGENTS
64
12.2.
EMBEDDING
MEDIUM
64
CONTENTS
VI
12.3.
METHOD
OF
EMBEDDING
64
13.
FACTORS
INFLUENCING
THE
CULTURE
OF
ARABIDOPSIS
PROTOPLASTS
65
13.1.
PRE-TREATMENT
65
13.2.
MEDIUM
65
13.3.
AUXINS
66
13.4.
CARBON
SOURCE
68
13.5.
ECOTYPES
68
13.6.
PROTOPLAST
PLATING
DENSITY
69
14.
REGENERATION
FROM
PROTOPLAST-DERIVED
COLONIES
70
14.1.
THE
INFLUENCE
OF
MEDIUM
70
14.2.
THE
INFLUENCE
OF
ECOTYPE
72
15.
SUMMARY:
NEW
PROTOCOL
OF
FAST
REGENERATION
FROM
PROTOPLASTS
OF
A.
THALIANA
73
DISCUSSION
1.
FACTORS
INFLUENCING
SOMATIC
EMBRYOGENESIS:
INTRINSIC
AND
EXTRINSIC
ASPECTS
74
2.
IDENTIFICATION
OF
A
GENE
WHICH
CONTROLS
SOMATIC
EMBRYOGENESIS
IN
A.
THALIANA
77
3.
MORPHOLOGY
AND
GERMINATION
OF
SOMATIC
EMBRYOS
78
4.
THE
TISSUE
AND
CELL
ORIGINS
OF
SOMATIC
EMBRYOS
80
5.
INITIAL
STAGES
OF
SOMATIC
EMBRYOGENESIS
IN
TISSUES
DERIVED
FROM
IMMATURE
ZYGOTIC
EMBRYOS
AND
LEAF
PROTOPLASTS
82
6.
USE
OF
A
MOLECULAR
MARKER
FOR
THE
STUDY
OF
EARLY
SOMATIC
EMBRYOGENESIS
84
7.
SOMATIC
EMBRYOGENESIS
IN
PATTERN
FORMATION
MUTANTS
85
8.
PROTOPLAST
CULTURE
OF
A.
THALIANA
87
9.
PERSPECTIVES
90
REFERENCES
91
APPENDIX
LIST
OF
TABLES
101
LIST
OF
FIGURES
102
CURRICULUM
VITAE
104
ACKNOWLEDGEMENTS
105 |
| any_adam_object | 1 |
| author | Luo, Yingkun 1964- |
| author_GND | (DE-588)118083651 |
| author_facet | Luo, Yingkun 1964- |
| author_role | aut |
| author_sort | Luo, Yingkun 1964- |
| author_variant | y l yl |
| building | Verbundindex |
| bvnumber | BV011609240 |
| ctrlnum | (OCoLC)258062822 (DE-599)BVBBV011609240 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV011609240</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">971104s1997 ad|| m||| 00||| engod</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">952676818</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)258062822</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV011609240</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-19</subfield><subfield code="a">DE-29T</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Luo, Yingkun</subfield><subfield code="d">1964-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)118083651</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Studies on arabidopsis thaliana cells in vitro</subfield><subfield code="b">somatic embryogenesis and protoplast culture</subfield><subfield code="c">von Yingkun Luo</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1997</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">VI, 105 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">München, Univ., Diss., 1997</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Zellkultur</subfield><subfield code="0">(DE-588)4067547-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Somatische Embryogenese</subfield><subfield code="0">(DE-588)4319974-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Ackerschmalwand</subfield><subfield code="0">(DE-588)4141299-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Ackerschmalwand</subfield><subfield code="0">(DE-588)4141299-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Zellkultur</subfield><subfield code="0">(DE-588)4067547-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Somatische Embryogenese</subfield><subfield code="0">(DE-588)4319974-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007819422&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-007819422</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV011609240 |
| illustrated | Illustrated |
| indexdate | 2024-08-18T00:08:18Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007819422 |
| oclc_num | 258062822 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-29T DE-12 DE-355 DE-BY-UBR DE-11 DE-188 |
| owner_facet | DE-19 DE-BY-UBM DE-29T DE-12 DE-355 DE-BY-UBR DE-11 DE-188 |
| physical | VI, 105 S. Ill., graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| record_format | marc |
| spelling | Luo, Yingkun 1964- Verfasser (DE-588)118083651 aut Studies on arabidopsis thaliana cells in vitro somatic embryogenesis and protoplast culture von Yingkun Luo 1997 VI, 105 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 1997 Zellkultur (DE-588)4067547-6 gnd rswk-swf Somatische Embryogenese (DE-588)4319974-4 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Zellkultur (DE-588)4067547-6 s Somatische Embryogenese (DE-588)4319974-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007819422&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Luo, Yingkun 1964- Studies on arabidopsis thaliana cells in vitro somatic embryogenesis and protoplast culture Zellkultur (DE-588)4067547-6 gnd Somatische Embryogenese (DE-588)4319974-4 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| subject_GND | (DE-588)4067547-6 (DE-588)4319974-4 (DE-588)4141299-0 (DE-588)4113937-9 |
| title | Studies on arabidopsis thaliana cells in vitro somatic embryogenesis and protoplast culture |
| title_auth | Studies on arabidopsis thaliana cells in vitro somatic embryogenesis and protoplast culture |
| title_exact_search | Studies on arabidopsis thaliana cells in vitro somatic embryogenesis and protoplast culture |
| title_full | Studies on arabidopsis thaliana cells in vitro somatic embryogenesis and protoplast culture von Yingkun Luo |
| title_fullStr | Studies on arabidopsis thaliana cells in vitro somatic embryogenesis and protoplast culture von Yingkun Luo |
| title_full_unstemmed | Studies on arabidopsis thaliana cells in vitro somatic embryogenesis and protoplast culture von Yingkun Luo |
| title_short | Studies on arabidopsis thaliana cells in vitro |
| title_sort | studies on arabidopsis thaliana cells in vitro somatic embryogenesis and protoplast culture |
| title_sub | somatic embryogenesis and protoplast culture |
| topic | Zellkultur (DE-588)4067547-6 gnd Somatische Embryogenese (DE-588)4319974-4 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| topic_facet | Zellkultur Somatische Embryogenese Ackerschmalwand Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007819422&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT luoyingkun studiesonarabidopsisthalianacellsinvitrosomaticembryogenesisandprotoplastculture |