Studies on the synthesis and storage of citrate in CAM plants: = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Mikrofilm Buch |
| Sprache: | German |
| Veröffentlicht: |
1996
|
| Ausgabe: | [Mikrofiche-Ausg.] |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Darmstadt, Techn. Hochsch., Diss., 1997.- Mikrofiche-Ausg.: 1 Mikrofiche : 24x |
| Beschreibung: | 83 Bl. graph. Darst. |
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| 245 | 1 | 0 | |a Studies on the synthesis and storage of citrate in CAM plants |b = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen |c vorgelegt von Iraya Güerere Quiroz |
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Datensatz im Suchindex
| _version_ | 1812453323097243648 |
|---|---|
| adam_text |
INDEX
1. INTRODUCTION.3
2. MATERIALS AND METHODS.7
2.1 PLANT MATERIAL.
.
7
2.2 ORGANIC ACIDS DETERMINATION
.
7
2.3 MEASUREMENT OF C0
2 EXCHANGE PATTERNS
.
8
\
2.4 LABELLING EXPERIMENTS WITH
13C02
.
8
2.4.1 APPLICATION OF
13C02, EXTRACTION AND CONVERSION OF ORGANIC ACIDS INTO TRIMETHYLSILYL
(TMS)-DERIVATIVES:.8
2.4.2 GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS):.9
2.4.3 CALCULATION OF MITOCHONDRIAL MALATE FLUX:.11
2.4.4 VARIATION OF THE EXTERNAL
13C02 AIR PRESSURE:.11
2.4.5 VARIATION OF THE TEMPERATURE:.12
2.4.6 VARIATION OF THE LIGHT INTENSITY:.12
2.4.7 TIME POINTS OF LABELLING DURING THE DIURNAL CAM CYCLE.-.12
2.5 ESTIMATION OF PARAMETERS CHARACTERISING THE NIGHTLY CARBON
METABOLISM:
.
13
2.6 INCUBATION WITH ACETATE
.
14
2.6.1 INCUBATION:.14
2.6.2 PROCESSING OF LABELLED SAMPLES AND MEASUREMENT OF
RADIOACTIVITY:.15
2.7 ANALYSIS OF THE ADENYLATE SYSTEM COMPONENTS
.
15
2.7A
COLLECTION AND TREATMENT OF THE PLANT MATERIAL:.15
2.7.2 MEASUREMENTS OF NUCLEOTIDES LEVEL:.16
2.7.3 CALCULATION OF THE NUCLEOTIDES CONCENTRATION AND ADENYLATE ENERGY
CHARGE (AEC):.17
3. RESULTS.18
3.1 PHYSIOLOGICAL CHARACTERISATION OF THE PLANTS: NET C0
2 EXCHANGE PATTERNS, NOCTURNAL MALATE AND
CITRATE ACCUMULATION
.
18
3.1.1 MALATE AND CITRATE ACCUMULATION UNDER NORMAL CONDITIONS.18
3.1.2 DAILY C0
2 EXCHANGE COURSES UNDER NORMAL CONDITIONS:.19
3.1.3 TEMPERATURE RESPONSE OF CUMULATIVE DARK C0
2 FIXATION, A MALATE AND A CITRATE:.21
3.2
13C02 -LABELLING PATTERNS AND INTRAMOLECULAR LABEL DISTRIBUTION
.
23
3.2.1 BEHAVIOUR OF PLANTS LABELLED UNDER NORMAL EXTERNAL CONDITIONS:.23
3.2.2 VARIATION OF THE EXTERNAL
13C02 PRESSURE:.27
3.2.3 VARIATION OF THE LIGHT INTENSITY:.30
3.2.4 VARIATION OF THE TEMPERATURE:.32
3.3 LABELLING PATTERNS AFTER APPLICATION OF
14C- ACETATE
.-.
36
3.3.1
14C-LABEL DISTRIBUTION AFTER LABELLING UNDER STANDARD CONDITIONS.36
3.3.2 DEPENDENCE ON THE TEMPERATURE:.38
3.4 INCUBATION IN NON-RADIOACTIVE ACETATE
.
44
3.4.1 VARIATION OF THE EXTERNAL ACETATE CONCENTRATION.44
3.5 MEASUREMENTS OF ATP, ADP AND AMP LEVELS
.
43
3.5.1 DIURNAL COURSES UNDER STANDARD CONDITIONS.43
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/950540137
3.5
MEASUREMENTS OF A TP, ADP AND AMP LEVELS.
3
.5.1
DIURNAL COURSES UNDER STANDARD CONDITIONS.
3
.5.2
DIURNAL COURSES OF THE ADENYLATE NUCLEOTIDE LEVELS UNDER VARIATION OF
NIGHT TEMPERATURE
4. DISCUSSION.
4
.1 EFFECTS OF C02 CONCENTRATIONS.
4.2 DIURNAL OSCILLATION OF ORGANIC ACIDS
.
4.3 CITRATE BIOSYNTHESIS
.
4.4 EFFECTS OF LIGHT
.
4
.5 EFFECTS OF OF EXTERNAL ACETATE.
4.6
F4C]-ACETATE DARKNESS INCUBATION.
4.7
EFFECTS OF VARIATION OF THE EXTERNAL ACETATE CONCENTRATION.
4.8 EFFECT OF TEMPERATURE MODIFICATIONS
.
4.9 METABOLITE TRANSPORT INTO THE MITOCHONDRIA
.
VARIATION IN THE FLUIDITY OF THE MITOCHONDRIAL MEMBRANE.~.
MITOCHONDRIAL CARRIERS.
4
.10 CARBON BALANCE.
4.11 CONSIDERATIONS ON THE ENERGY BUDGET.
.
5
.
SUMMARY.
6. REFERENCES.
7. APPENDICES.
APPENDIX 1: ABREVIATIONS
.
APPENDIX 2: NUMBERING OF THE CARBONS IN THE MALATE AND CITRATE
MOLECULES
.
APPENDIX 3: POSSIBLE NIGHTLY AMINOACIDS BIOSYNTHESIS PATHWAYS IN CAM.
8. DANKSAGUNGEN.
9. EIDESSTATTLICHE ERKLARUNG.
10. RESUME.
.
43
.43
.45
.48
.
48
.50
. 54
. 56
. 58
. 58
.59
.60
.63
.64
.64
.67
.69
.70
.72
.81
.81
.83
84
.85
.86
.87 |
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| author | Güerere Quiroz, Iraya 1966- |
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| edition | [Mikrofiche-Ausg.] |
| format | Microfilm Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
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| id | DE-604.BV011606035 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T16:09:42Z |
| institution | BVB |
| language | German |
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| physical | 83 Bl. graph. Darst. |
| publishDate | 1996 |
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| spelling | Güerere Quiroz, Iraya 1966- Verfasser (DE-588)115420649 aut Studies on the synthesis and storage of citrate in CAM plants = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen vorgelegt von Iraya Güerere Quiroz Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen [Mikrofiche-Ausg.] 1996 83 Bl. graph. Darst. txt rdacontent h rdamedia he rdacarrier Darmstadt, Techn. Hochsch., Diss., 1997.- Mikrofiche-Ausg.: 1 Mikrofiche : 24x CAM-Pflanzen (DE-588)4351679-8 gnd rswk-swf Citronensäure (DE-588)4148057-0 gnd rswk-swf Akkumulation (DE-588)4000912-9 gnd rswk-swf Biosynthese (DE-588)4006902-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content CAM-Pflanzen (DE-588)4351679-8 s Citronensäure (DE-588)4148057-0 s Biosynthese (DE-588)4006902-3 s DE-604 Akkumulation (DE-588)4000912-9 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007818395&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Güerere Quiroz, Iraya 1966- Studies on the synthesis and storage of citrate in CAM plants = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen CAM-Pflanzen (DE-588)4351679-8 gnd Citronensäure (DE-588)4148057-0 gnd Akkumulation (DE-588)4000912-9 gnd Biosynthese (DE-588)4006902-3 gnd |
| subject_GND | (DE-588)4351679-8 (DE-588)4148057-0 (DE-588)4000912-9 (DE-588)4006902-3 (DE-588)4113937-9 |
| title | Studies on the synthesis and storage of citrate in CAM plants = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen |
| title_alt | Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen |
| title_auth | Studies on the synthesis and storage of citrate in CAM plants = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen |
| title_exact_search | Studies on the synthesis and storage of citrate in CAM plants = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen |
| title_full | Studies on the synthesis and storage of citrate in CAM plants = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen vorgelegt von Iraya Güerere Quiroz |
| title_fullStr | Studies on the synthesis and storage of citrate in CAM plants = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen vorgelegt von Iraya Güerere Quiroz |
| title_full_unstemmed | Studies on the synthesis and storage of citrate in CAM plants = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen vorgelegt von Iraya Güerere Quiroz |
| title_short | Studies on the synthesis and storage of citrate in CAM plants |
| title_sort | studies on the synthesis and storage of citrate in cam plants studien uber die synthese und akkumulation von zitronensaure in cam pflanzen |
| title_sub | = Studien über die Synthese und Akkumulation von Zitronensäure in CAM-Pflanzen |
| topic | CAM-Pflanzen (DE-588)4351679-8 gnd Citronensäure (DE-588)4148057-0 gnd Akkumulation (DE-588)4000912-9 gnd Biosynthese (DE-588)4006902-3 gnd |
| topic_facet | CAM-Pflanzen Citronensäure Akkumulation Biosynthese Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007818395&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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