Lectin methods and protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, New Jersey
Humana Press
1998
|
| Schriftenreihe: | Methods in molecular medicine
9 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVIII, 616 S. Ill., graph. Darst. |
| ISBN: | 0896033961 |
Internformat
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| 245 | 1 | 0 | |a Lectin methods and protocols |c ed. by Jonathan M. Rhodes ... |
| 264 | 1 | |a Totowa, New Jersey |b Humana Press |c 1998 | |
| 300 | |a XVIII, 616 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804126126506770432 |
|---|---|
| adam_text | Contents
Preface v
Contributors xiii
Part I. Lectin Histochemistry and Cytochemistry
A. Light Microscopy
1 Light Microscopy: Overview and Basic Methods
Anthony J. Leathern and Susan A. Brooks 3
2 Lectin Histochemistry and Cytochemistry—Light Microscopy:
Avidin Biotin Amplification on Resin Embedded Sections
Robert W. Stoddart and Carolyn J. P. Jones 21
3 Lectin Gold Histochemistry on Paraffin and Lowicryl K4M
Sections Using Biotin and Digoxigenin Conjugated Lectins
Jurgen Roth, Christian Zuber, Tetsutaro Sata, and Wei Ping Li... 41
4 Use of Fluorochrome Labeled Lectins in Light Microscopy
Udo Schumacher and Barry S. Mitchell 55
5 The Use of Lectins in Combination with Enzymatic Digestion
for the Study of Glycoconjugates in Cartilage
Sibylle Hoedt Schmidt 65
6 Applications of Lectin Histochemistry and Cytochemistry
in Diagnosis and Prognosis
Timothy Richard Helliwell 73
B. Electron Microscopy
7 Electron Microscopy: Use of Lectin Peroxidase Prior to Embedding
Adi Ellinger 97
8 Electron Microscopy: Use of Lectin Gold After Embedding
Rainer Herken and Berti Manshausen /11
9 Amplification of Lectin Gold Histochemistry
Juan F. Madrid, Francisco Hernandez, and Jose Ballesta 121
10 Electron Microscopic Methods for the Demonstration
of Lectin Binding Sites in Cancer Cell Lines
Barry S. Mitchell and Udo Schumacher 133
vii
viii Contents
Part II. Use of Lectins for Structural Analysis of Oligosaccharide Chains
11 Application of Sequential Smith Degradation to Lectin Blots
Chi Kong Ching 147
12 Blot Analysis with Lectins for the Evaluation of Glycoproteins
in Cultured Cells and Tissues
Christian Zuber, Wei Ping Li, and Jurgen Roth 159
13 Characterization of HIV gp120 Envelope Glycoprotein
by Lectin Analysis
Gregers J. Gram and John Erik Stig Hansen 167
14 Use of Lectins for Characterization of O Linked Glycans
of Herpes Simplex Virus Glycoproteins
Sigvard Olofsson and Anders Bolmstedt 175
Part III. Lectins for Detection of Altered Glycosylation of Circulating
Glycoproteins
15 Use of Lectin for Detection of Agalactosyl IgG
Naoyuki Tsuchiya, Tamao Endo, Naohisa Kochibe, Koji Ito,
and Akira Kobata 195
16 Lectins for Detection of Altered Glycosylation of Circulating
Glycoproteins: a 1 Antitrypsin
Yutaka Aoyagi and Hitoshi Asakura 207
17 Detection of Altered Glycosylation of a Fetoprotein Using
Lectin Affinity Electrophoresis
Kazuhisa Taketa, Miao Liu, and Hiroko Taga 215
18 Use of Lectin Affinity Electrophoresis for Quantification
and Characterization of Glycoforms of a 1 Acid Glycoprotein
Thorkild C. Bog Hansen 227
19 ABO(H) Blood Group Expression on Circulating Glycoproteins
Taei Matsui and Koiti Titani 235
Part IV. Use of Lectins in Quantification of Soluble Glycoproteins
20 Lectin/Antibody Sandwich ELISA for Quantification of Circulating
Mucin as a Diagnostic Test for Pancreatic Cancer
Neil Parker 249
21 Quantification of Intestinal Mucins
Jeremy D. Milton and Jonathan M. Rhodes 255
Contents ix
Part V. Lectins in Affinity Purification of Soluble Glycoproteins
22 Purification and Characterization of Human Serum and Secretory
lgA1 and lgA2 Using Jacalin
Michael A. Kerr, Lesley M. Loomes, Brian C. Bonner,
Amy B. Hutchings, and Bernard W. Senior 265
23 Use of Lectins in Affinity Purification of HIV and SIV Envelope
Glycoproteins
Gustav Gilljam 279
24 T Cell Receptor Purification
Kelly P. Kearse 291
Part VI. Lectins in Flow Cytometry
25 Use of Monomeric, Monovalent Lectin Derivatives for Flow
Cytometric Analysis of Cell Surface Glycoconjugates
Hanae Kaku and Naoto Shibuya 301
26 Analysis of Subcellular Components by Fluorescent Lectin Binding
and Flow Cytometry
Rosa M. Guasch and Jose Enrique O Connor 307
Part VII. Lectins as Tools for Cell Purification/Purging
27 Lectins as Tools for the Purification of Liver Endothelial Cells
Daniel E. Gomez and Unnur P. Thorgeirsson 319
28 The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM)
Purging and Hematopoietic Progenitor Cell Enrichment
in Clinical Bone Marrow Transplantation
A rnon Nagler, Shoshana Morecki, and Shimon Sla vin 329
29 Combined Lectin/Monoclonal Antibody Purging of Bone Marrow
for Use in Conjunction with Autologous Bone Marrow
Transplantation in the Treatment of Multiple Myeloma
Elizabeth G. H. Rhodes 351
Part VIII. Effects of Lectins on Mammalian Cells
30 Mechanisms and Assessment of Mitogenesis: An Overview
David C. Kilpatrick 365
31 Mitogenic Effects of Lectins on Epithelial Cells
Lu Gang Yu and Jonathan M. Rhodes 379
x Contents
32 Use of Lectins as Mitogens for Lymphocytes
David C. Kilpatrick 385
33 Effect of Lectins on Uptake of Polyamines
Susan Bardocz and Ann White 393
34 Effects of Lectins on Cytoskeletal Organization in Mammalian Cells
Paolo Carinci, Ennio Becchetti, and Maria Bodo 407
35 Effect of Lectins on Protein Kinase Activity
Kiyonao Sada and Hirohei Yamamura 423
36 Lectin lnduced Calcium Mobilization in Human Platelets:
Use of Fluorescent Probes
Giuseppe Ramaschi and Mauro Torti 433
37 Lectin Triggered Superoxide/H2O2 and Granule Enzyme Release
from Cells
Alexander V. Timoshenko, Klaus Kayser,
and Hans Joachim Gabius 441
38 Cytotoxic Effects of Lectins
Elieser Gorelik 453
39 Application of the Lectin Dependent Cell Mediated Cytoxicity
Assay to Bronchoalveolar Lavage Fluid and Venous Blood
Samples Collected from Canine Lung Allografts
Allan G. L. Lee and Hani A. Shennib 461
Part IX. Effects of Lectins in Organ Culture
40 The Effect of Lectins on Crypt Cell Proliferation in Organ Culture
Stephen D. Ryder 475
Part X. Effects of Lectin Ingestion
41 Effects of Lectin Ingestion on Animal Growth and Internal Organs
Arpad Pusztai 485
42 Lectin Ingestion: Changes in Mucin Secretion and Bacterial
Adhesion to Intestinal Tissue
Howard Ceri, John G. Banwell, and Rixun Fang 495
43 Assessment of Lectin Inactivation by Heat and Digestion
Arpad Pusztai and George Grant 505
Part XI. Lectins in the Investigation of Neuronal Trafficking
44 Use of Lectins as Transganglionic Neuronal Tracers in the Study
of Unmyelinated Primary Sensory Neurons
Mark B. Plenderleith and Peter J. Snow 517
Contents xi
Part XII. Use of Lectins in the Investigation of Pathogen—Host
Interactions
45 Lectin Inhibition of Bacterial Adhesion to Animal Cells
Murray W. Stinson and Jen Ren Wang 529
46 Inhibition of HIV Infection by Lectin Binding to CD4
Jean Favero and Virginie Lafont 539
47 Inhibition of HIV Infection by Lectin Binding to gp120
Theresa Animashaun and Naheed Mahmood 555
Part XIII. Use of Lectins for Drug Delivery
48 Absorption Enhancement by Lectin Mediated
Endo and Transcytosis
Ellen Haltner, Gerrit Borchard, and Claus Michael Lehr 567
49 The Use of Lectins for Liposome Targeting in Drug Delivery
Michael Kaszuba and Malcolm N. Jones 583
Appendix—List of Lectins and Binding Structures 595
Index 599
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| spelling | Lectin methods and protocols ed. by Jonathan M. Rhodes ... Totowa, New Jersey Humana Press 1998 XVIII, 616 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 9 Lectinas larpcal Lectinen gtt Lectines - Analyse ram Lectines ram Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Lectins Lectins Analysis Lectins analysis Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Analyse (DE-588)4122795-5 gnd rswk-swf Lectine (DE-588)4034971-8 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Lectine (DE-588)4034971-8 s Molekularbiologie (DE-588)4039983-7 s Analyse (DE-588)4122795-5 s DE-604 Rhodes, Jonathan M. Sonstige oth Methods in molecular medicine 9 (DE-604)BV011334960 9 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007812067&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lectin methods and protocols Methods in molecular medicine Lectinas larpcal Lectinen gtt Lectines - Analyse ram Lectines ram Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Lectins Lectins Analysis Lectins analysis Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Analyse (DE-588)4122795-5 gnd Lectine (DE-588)4034971-8 gnd |
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| title | Lectin methods and protocols |
| title_auth | Lectin methods and protocols |
| title_exact_search | Lectin methods and protocols |
| title_full | Lectin methods and protocols ed. by Jonathan M. Rhodes ... |
| title_fullStr | Lectin methods and protocols ed. by Jonathan M. Rhodes ... |
| title_full_unstemmed | Lectin methods and protocols ed. by Jonathan M. Rhodes ... |
| title_short | Lectin methods and protocols |
| title_sort | lectin methods and protocols |
| topic | Lectinas larpcal Lectinen gtt Lectines - Analyse ram Lectines ram Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Lectins Lectins Analysis Lectins analysis Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Analyse (DE-588)4122795-5 gnd Lectine (DE-588)4034971-8 gnd |
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