Microinjection and transgenesis: strategies and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
1998
|
| Schriftenreihe: | Springer lab manual
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 619 S. Ill., graph. Darst. |
| ISBN: | 3540618953 |
Internformat
MARC
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| 245 | 1 | 0 | |a Microinjection and transgenesis |b strategies and protocols |c Angel Cid-Arregui ... (eds.) |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 1998 | |
| 300 | |a XIII, 619 S. |b Ill., graph. Darst. | ||
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| 650 | 4 | |a Animals, Genetically Modified |v Laboratory Manuals | |
| 650 | 4 | |a Genetic engineering |v Laboratory manuals | |
| 650 | 4 | |a Microinjections |v Laboratory Manuals | |
| 650 | 4 | |a Microinjections |v Laboratory manuals | |
| 650 | 4 | |a Transgenes |v Laboratory Manuals | |
| 650 | 4 | |a Transgenic animals |v Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804126097119379456 |
|---|---|
| adam_text | Contents
Parti
Strategies and Protocols
for Microinjection Experiments Using Somatic Cells in Culture... 1
Chapter 1
Protocols for the Manual and Automatic Microinjection of Somatic
Cells in Culture and for the Analysis of Microinjected Cells 3
Angel Cid Arregui, Carla Santana, Miriam Guido,
Nestor Morales Peza, Maria Victoria Juarez, Roger Wepf,
and Alejandro Garcia Carranca
Chapter 2
Automated Computer Assisted Microinjection
into Cultured Somatic Cells 31
WlLHELM ANSORGE AND RAINER SAFFRICH
Chapter 3
Immunofluorescence and Immunoelectron Microscopy
of Microinjected and Transfected Cultured Cells 47
Gustavo Egea, Teresa Babia, Roser Pagan, Roser Busca,
Inmaculada Ayala Ferran Valderrama, Manuel Reina,
AND SENEN VlLARO
Chapter 4
Videomicroscopy of Living Microinjected Hippocampal Neurons
in Culture 81
Frank Bradke and Carlos G. Dotti
X Contents
Chapter 5
Preparation, Purification, and Analysis of Synthetic
Oligonucleotides Suitable for Microinjection Experiments 95
Ramon Guimil Garcia and Ramon Eritja
Chapter 6
Colorimetric in Situ Hybridization 113
Jaime Berumen
Chapter 7
Manual Microinjection: Measuring the Inhibitory Effect
of HIV 1 Directed Hammerhead Ribozymes in Tissue Culture
Cells 131
Robert Hormes and Georg Sczakiel
Chapter 8
Cellular Microbiochemistry 145
Rainer Pepperkok, Olaf Rosorius, and Jochen Scheel
Chapter 9
A New Strategy for Studying Microtubule Transport
and Assembly During Axon Growth 155
Peter W. Baas
Chapter 10
The Use of Microinjection to Study Signal Transduction
in Mammalian Cells 171
Serge Roche and Sara A. Courtneidge
Part 2
Strategies and Protocols
for Microinjection Experiments Using Embryos 185
Chapter 11
Supply and Husbandry of Mice for Transgenic Science 187
Jane Morrell
Contents XI
Chapter 12
Mouse Male Germ Cells in Culture:
Toward a New Approach in Transgenesis? 217
Minoo Rassoulzadegan, Julien Sage, and Valerie Grandjean
Chapter 13
Sperm Mediated Gene Transfer 229
Marialuisa Lavitrano, Valentina Lulli, Barbara Maione,
Sabina Sperandio, and Corrado Spadafora
Chapter 14
Application of Ligand Dependent Site Specific Recombination
in ES Cells 255
Pierre Olivier Angrand, Catherine P. Woodroofe,
and A. Francis Stewart
Chapter 15
A Modified Protocol for the Generation of Transgenic Mice
and Freezing of Mouse Embryos 275
Carol Murphy, Luis Martin Parras, and Ulrich Ruther
Chapter 16
The Use of Morula Aggregation to Generate Germline Chimeras
from Genetically Modified Embryonic Stem Cells 285
Miguel Torres
Chapter 17
The Generation
of Transgenic Mice with Yeast Artificial Chromosomes 297
Holger Hiemisch, Thorsten Umland, Lluis Montoliu,
and gtjnther schutz
Chapter 18
Following Up Microinjected mRNAs: Analysis of mRNA Poly(A)
Tail Length During Mouse Oocyte Maturation 309
Fatima Gebauer
j
XII Contents
Chapter 19
Mouse Preimplantation Embryos and Oocytes
as an In Vivo System to Study Transcriptional Enhancers 323
Sadhan Majumder
Chapter 20
The Use of Mouse Preimplantation Embryos for the Identification
of DNA Binding Proteins Essential in Early Development 351
Valerie Botquin, Jorg R. Schlehofer, and Angel Cid Arregui
Chapter 21
Application of Transgenes and Transgenic Mice to Study Gene
Activity from the Oocyte to the Early Embryo 369
Sylvie Forlani, Lucile Montfort, and Jean Francois Nicolas
Chapter 22
Transitory Transgenic Analysis as an In Vivo System
to Study Promoter Regulatory Elements 413
Diana Escalante Alcalde and Luis Covarrubias
Chapter 23
Transgenic Analysis of Embryonic Gene Expression Using LacZ
as a Reporter 427
Catherine E. Ovitt, Young II Yeom, and Hans R. Scholer
Chapter 24
Autonomous Cell Labeling Using a laacZ Reporter Transgene
to Produce Genetic Mosaics During Development 439
Luc Mathis and Jean Francois Nicolas
Chapter 25
Multiple Developmental Functions
of the Zinc Finger Gene Krox 20 Are Revealed by Targeted Insertion
of the E. coli lacZ Coding Sequence 459
Sylvie Schneider Maunoury, Piotr Topilko, j
Tania Seitanidou, Giovanni Levi, Paula Murphy, J
and Patrick Charnay
Contents XIII
Chapter 26
Transgenic Mouse Strategies in Virus Research 477
Angel Cid Arregui, Nestor Morales Peza,
Prasert Auewarakul, Maria Jose Garcia Iglesias,
Maria Victoria Juarez, Gina Diaz,
and Alejandro Garcia Carranca
Chapter 27
Isolation of Mutated Genes from Transgene Insertion Sites 505
Miriam H. Meisler, James Galt, John S. Weber,
Julie M. Jones, Daniel L. Burgess, and David C. Kohman
Chapter 28
Contributions of Transgenic and Knockout Mice
to Immunological Knowledge 521
Jose Moreno, Laura C. Bonifaz,
and Jesus Martinez Barnetche
Chapter 29
Generation and Application of Transgenic Rabbits 561
Urban Besenfelder, Bernhard Aigner, Mathias Muller,
and Gottfried Brem
Chapter 30
Microinjection of Drosophila Eggs 587
Cristiana Mollinari and Cayetano Gonzalez
|
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| indexdate | 2024-07-09T18:12:02Z |
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| isbn | 3540618953 |
| language | English |
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| spelling | Microinjection and transgenesis strategies and protocols Angel Cid-Arregui ... (eds.) Berlin [u.a.] Springer 1998 XIII, 619 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer lab manual Animals, Genetically Modified Laboratory Manuals Genetic engineering Laboratory manuals Microinjections Laboratory Manuals Microinjections Laboratory manuals Transgenes Laboratory Manuals Transgenic animals Laboratory manuals Gentechnologie (DE-588)4071722-7 gnd rswk-swf Transgene Tiere (DE-588)4279021-9 gnd rswk-swf Gentransfer (DE-588)4194102-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Mikroinjektion (DE-588)4404677-7 gnd rswk-swf Transgene Tiere (DE-588)4279021-9 s Methode (DE-588)4038971-6 s DE-604 Mikroinjektion (DE-588)4404677-7 s Gentechnologie (DE-588)4071722-7 s Gentransfer (DE-588)4194102-0 s Cid Arregui, Angel 1959- Sonstige (DE-588)115479856 oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007791573&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Microinjection and transgenesis strategies and protocols Animals, Genetically Modified Laboratory Manuals Genetic engineering Laboratory manuals Microinjections Laboratory Manuals Microinjections Laboratory manuals Transgenes Laboratory Manuals Transgenic animals Laboratory manuals Gentechnologie (DE-588)4071722-7 gnd Transgene Tiere (DE-588)4279021-9 gnd Gentransfer (DE-588)4194102-0 gnd Methode (DE-588)4038971-6 gnd Mikroinjektion (DE-588)4404677-7 gnd |
| subject_GND | (DE-588)4071722-7 (DE-588)4279021-9 (DE-588)4194102-0 (DE-588)4038971-6 (DE-588)4404677-7 |
| title | Microinjection and transgenesis strategies and protocols |
| title_auth | Microinjection and transgenesis strategies and protocols |
| title_exact_search | Microinjection and transgenesis strategies and protocols |
| title_full | Microinjection and transgenesis strategies and protocols Angel Cid-Arregui ... (eds.) |
| title_fullStr | Microinjection and transgenesis strategies and protocols Angel Cid-Arregui ... (eds.) |
| title_full_unstemmed | Microinjection and transgenesis strategies and protocols Angel Cid-Arregui ... (eds.) |
| title_short | Microinjection and transgenesis |
| title_sort | microinjection and transgenesis strategies and protocols |
| title_sub | strategies and protocols |
| topic | Animals, Genetically Modified Laboratory Manuals Genetic engineering Laboratory manuals Microinjections Laboratory Manuals Microinjections Laboratory manuals Transgenes Laboratory Manuals Transgenic animals Laboratory manuals Gentechnologie (DE-588)4071722-7 gnd Transgene Tiere (DE-588)4279021-9 gnd Gentransfer (DE-588)4194102-0 gnd Methode (DE-588)4038971-6 gnd Mikroinjektion (DE-588)4404677-7 gnd |
| topic_facet | Animals, Genetically Modified Laboratory Manuals Genetic engineering Laboratory manuals Microinjections Laboratory Manuals Microinjections Laboratory manuals Transgenes Laboratory Manuals Transgenic animals Laboratory manuals Gentechnologie Transgene Tiere Gentransfer Methode Mikroinjektion |
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