Analysis of nucleic acids by capillary electrophoresis:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Braunschweig [u.a.]
Vieweg
1997
|
| Schriftenreihe: | Chromatographia
1 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 313 S. Ill., graph. Darst. |
| ISBN: | 352806871X |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV011408245 | ||
| 003 | DE-604 | ||
| 005 | 19971008 | ||
| 007 | t | ||
| 008 | 970624s1997 gw ad|| |||| 00||| eng d | ||
| 016 | 7 | |a 950655503 |2 DE-101 | |
| 020 | |a 352806871X |9 3-528-06871-X | ||
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| 084 | |a WD 5300 |0 (DE-625)148199: |2 rvk | ||
| 245 | 1 | 0 | |a Analysis of nucleic acids by capillary electrophoresis |c Christoph Heller (ed.) |
| 264 | 1 | |a Braunschweig [u.a.] |b Vieweg |c 1997 | |
| 300 | |a X, 313 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Chromatographia |v 1 | |
| 650 | 4 | |a Capillary electrophoresis | |
| 650 | 4 | |a Electrophoresis, Capillary |x methods | |
| 650 | 4 | |a Nucleic Acids |x analysis | |
| 650 | 4 | |a Nucleic acids |x Analysis | |
| 650 | 4 | |a Sequence Analysis, DNA |x methods | |
| 650 | 4 | |a Sequence Analysis, RNA |x methods | |
| 650 | 0 | 7 | |a Nucleinsäuren |0 (DE-588)4172117-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Kapillarelektrophorese |0 (DE-588)4290002-5 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Nucleinsäuren |0 (DE-588)4172117-2 |D s |
| 689 | 0 | 1 | |a Kapillarelektrophorese |0 (DE-588)4290002-5 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Heller, Christoph |e Sonstige |4 oth | |
| 830 | 0 | |a Chromatographia |v 1 |w (DE-604)BV011568238 |9 1 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007670242&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
Datensatz im Suchindex
| _version_ | 1805067844485906432 |
|---|---|
| adam_text |
V
TABLE
OF
CONTENTS
TABLE
OF
CONTENTS.
-----.-----.
-
.
_
.
-
.
V
PREFACE
.VIN
CONTRIBUTORS.IX
PART
I
BASIC
CONCEPTS.
1
1
THE
SEPARATION
MATRIX.
-
.
3
1.1
INTRODUCTION
.
3
1.2
CAPILLARY
ELECTROPHORESIS
.
4
1.3
PROPERTIES
OF
POLYMERS
.
5
1.4
DESIGNING
A
SEPARATION
MATRIX
.
10
1.5
PREPARATION
OF
GELS
AND
POLYMER
SOLUTIONS
.
15
1.6
CONCLUSIONS
.
18
ACKNOWLEDGEMENT.
18
REFERENCES
.
18
2
ELECTROPHORESIS
THEORIES.
24
2.1
INTRODUCTION
.
24
2.2
TRANSPORT
PHENOMENA
AND
SEPARATION
PROCESSES
.
26
2.3
NUCLEIC
ACIDS
IN
FREE
SOLUTIONS
.
29
2.4
NUCLEIC
ACIDS
IN
GELS
.
32
2.5
NUCLEIC
ACIDS
IN
ENTANGLED
POLYMER
SOLUTIONS
.
47
2.6
NUCLEIC
ACIDS
IN
DILUTE
POLYMER
SOLUTIONS
.
49
2.7
OTHER
TOPICS
OF
INTEREST
.
53
2.8
CONCLUSION
.
54
ACKNOWLEDGEMENTS
.
55
REFERENCES
.
56
GLOSSARY
OF
ABBREVIATIONS,
SYMBOLS,
AND
COMMON
UNITS
.
65
3
MICROSCOPIC
STUDIES
ON
THE
MIGRATION
MECHANISM
.67
3.1
INTRODUCTION
.
67
3.2
FLUORESCENCE
MICROSCOPY
.
68
3.3
STUDIES
IN
AGAROSE
GEL
.
73
3.4
STUDIES
IN
POLYMER
SOLUTIONS
.
76
3.5
STUDIES
IN
FREE
SOLUTION
.
85
REFERENCES
.
85
VI
TABLE
OF
CONTENTS
PART
II
FACTORS
AFFECTING
THE
SEPARATION.
91
4
INFLUENCE
OF
POLYMER
CONCENTRATION
AND
ELECTRIC
FIELD.
93
4.1
INTRODUCTION
.
93
4.2
DNA
MOBILITY
AS
A
FUNCTION
OF
POLYMER
CONCENTRATION,
MOLECULAR
WEIGHT
AND
APPLIED
ELECTRIC
FIELD:
.
98
4.3
CONSEQUENCES
FOR
THE
USER
.
119
REFERENCES
.
120
5
EFFECTS
OF
SAMPLE
MATRIX
AND
INJECTION.
125
5.1
INJECTION
ISSUES
.
125
5.2
TECHNIQUES
TO
OVERCOME
SALTY
SAMPLES
.
128
5.3
CONCLUSIONS
AND
FUTURE
DIRECTIONS
.
132
ACKNOWLEDGEMENTS
.
133
REFERENCES
.
133
6
DEVELOPMENTS
IN
CAPILLARY
COATING
AND
DNA
SEPARATION
MATRICES.
135
6.1
INTRODUCTION
.
135
6.2
ELECTROOSMOTIC
FLOW
.
136
6.3
METHODS
TO
CONTROL
ELECTROOSMOTIC
FLOW
.
141
6.4
DYNAMIC
AND
ADSORBED
COATINGS
.
142
6.5
COVALENTLY
BONDED
PHASES
.
147
6.6
SIEVING
MATRICES
.
157
6.7
POLYACRYLAMIDE
POLYMERS
.
158
6.8
AGAROSE
.
162
6.9
CELLULOSIC
POLYMERS
.
164
6.10
POLY
(ETHYLENEOXIDE)
(PEO)
.
166
REFERENCES
.
167
PART
III
APPLICATIONS
175
7
ANALYSIS
OF
OLIGONUCLEOTIDES
AND
ANTISENSE
DNA
ANALOGS.
177
7.1
INTRODUCTION
.
177
7.2
SINGLE
STRANDED
OLIGONUCLEOTIDES
AND
ANTISENSE
COMPOUNDS
.
178
7.3
SIZE
SEPARATION
OF
OLIGONUCLEOTIDES
.
181
7.4.
APPLICATIONS
.
188
REFERENCES
.
192
8
SEPARATION
OF
DNA
RESTRICTION
FRAGMENTS
AND
PCR
PRODUCTS.
195
8.1
INTRODUCTION
.
195
8.2
DNA
RESTRICTION
FRAGMENT
ANALYSIS
.
200
8.3
PCR
PRODUCT
ANALYSIS
.
204
8.4
FUTURE
TRENDS
.
210
ACKNOWLEDGEMENTS
.
211
REFERENCES
.
212
TABLE
OF
CONTENTS
VII
9
PULSED-FIELD
CAPILLARY
ELECTROPHORESIS
OF
LARGE
DNA
.
218
9.1
INTRODUCTION
.
218
9.2
THEORY
.
220
9.3
MOBILITY
MINIMA
IN
PFCE
.
222
9.4
SEPARATION
OF
SMALL
DNA
MOLECULES
.
227
9.5
SEPARATION
OF
LARGE
DNA
MOLECULES
.
227
9.6
CONCLUSIONS
.
232
ACKNOWLEDGMENT
.
232
REFERENCES
.
232
10
CAPILLARY
ELECTROPHORESIS
FOR
DNA
SEQUENCING
.
236
10.1
INTRODUCTION
.
236
10.2
INSTRUMENTATION
-
SINGLE
CAPILLARY
.
237
10.3
INSTRUMENTATION
-
MULTIPLE
CAPILLARY
.
239
10.4
SEPARATION
MATRICES
.
244
10.5
INFLUENCE
OF
POLYMER
CONCENTRATION,
ELECTRIC
FIELD
AND
TEMPERATURE
.
246
10.6
FUTURE
PROSPECTS
.
251
ACKNOWLEDGMENTS
.
251
REFERENCES
.
251
11
MUTATIONAL
ANALYSIS
WITH
CAPILLARY
ELECTROPHORESIS
.255
11.1
INTRODUCTION
.
255
11.2
SIEVING
MATRICES
AND
POLYMER
NETWORKS
.
256
11.3
SEPARATION
OF
PCR
PRODUCTS
.
262
11.4
DETECTION
OF
DNA
POINT
MUTATIONS
.
265
11.5
CONCLUSIONS
.
267
ACKNOWLEDGEMENTS
.
269
REFERENCES
.
270
ABBREVIATIONS
.
273
12
ANALYSIS
OF
TRANSFER
RNA
AND
5S
RIBOSOMAL
RNA.
274
12.1
INTRODUCTION
.
274
12.2
SEPARATION
BY
ENTANGLED-POLYMER
CAPILLARY
ELECTROPHORESIS
.
275
12.3
SEPARATION
BY
CAPILLARY
GEL
ELECTROPHORESIS
.
287
12.4
METHODOLOGY
.
287
12.5
CONCLUSIONS
.
289
ACKNOWLEDGEMENTS
.
290
REFERENCES
.
290
PART
IV
RECENT
DEVELOPMENTS
.
295
13
UPDATE
ON
IMPROVEMENTS
IN
DNA
SEPARATION
.
297
13.1
INTRODUCTION
.
297
13.2
DEVELOPMENTS
IN
INSTRUMENTATION
AND
TECHNOLOGY
.
297
13.3
DIFFERENT
ASPECTS
OF
SEPARATION
.
301
REFERENCES
.
306
SUBJECT
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| id | DE-604.BV011408245 |
| illustrated | Illustrated |
| indexdate | 2024-07-20T03:40:40Z |
| institution | BVB |
| isbn | 352806871X |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007670242 |
| oclc_num | 38757286 |
| open_access_boolean | |
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| owner_facet | DE-355 DE-BY-UBR DE-703 DE-12 DE-11 |
| physical | X, 313 S. Ill., graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| publisher | Vieweg |
| record_format | marc |
| series | Chromatographia |
| series2 | Chromatographia |
| spelling | Analysis of nucleic acids by capillary electrophoresis Christoph Heller (ed.) Braunschweig [u.a.] Vieweg 1997 X, 313 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Chromatographia 1 Capillary electrophoresis Electrophoresis, Capillary methods Nucleic Acids analysis Nucleic acids Analysis Sequence Analysis, DNA methods Sequence Analysis, RNA methods Nucleinsäuren (DE-588)4172117-2 gnd rswk-swf Kapillarelektrophorese (DE-588)4290002-5 gnd rswk-swf Nucleinsäuren (DE-588)4172117-2 s Kapillarelektrophorese (DE-588)4290002-5 s DE-604 Heller, Christoph Sonstige oth Chromatographia 1 (DE-604)BV011568238 1 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007670242&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Analysis of nucleic acids by capillary electrophoresis Chromatographia Capillary electrophoresis Electrophoresis, Capillary methods Nucleic Acids analysis Nucleic acids Analysis Sequence Analysis, DNA methods Sequence Analysis, RNA methods Nucleinsäuren (DE-588)4172117-2 gnd Kapillarelektrophorese (DE-588)4290002-5 gnd |
| subject_GND | (DE-588)4172117-2 (DE-588)4290002-5 |
| title | Analysis of nucleic acids by capillary electrophoresis |
| title_auth | Analysis of nucleic acids by capillary electrophoresis |
| title_exact_search | Analysis of nucleic acids by capillary electrophoresis |
| title_full | Analysis of nucleic acids by capillary electrophoresis Christoph Heller (ed.) |
| title_fullStr | Analysis of nucleic acids by capillary electrophoresis Christoph Heller (ed.) |
| title_full_unstemmed | Analysis of nucleic acids by capillary electrophoresis Christoph Heller (ed.) |
| title_short | Analysis of nucleic acids by capillary electrophoresis |
| title_sort | analysis of nucleic acids by capillary electrophoresis |
| topic | Capillary electrophoresis Electrophoresis, Capillary methods Nucleic Acids analysis Nucleic acids Analysis Sequence Analysis, DNA methods Sequence Analysis, RNA methods Nucleinsäuren (DE-588)4172117-2 gnd Kapillarelektrophorese (DE-588)4290002-5 gnd |
| topic_facet | Capillary electrophoresis Electrophoresis, Capillary methods Nucleic Acids analysis Nucleic acids Analysis Sequence Analysis, DNA methods Sequence Analysis, RNA methods Nucleinsäuren Kapillarelektrophorese |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007670242&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV011568238 |
| work_keys_str_mv | AT hellerchristoph analysisofnucleicacidsbycapillaryelectrophoresis |