Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
1996
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Heidelberg, Univ., Diss., 1997 (Nicht für den Austausch) |
| Beschreibung: | IV, 113 S. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain |c vorgelegt von Tsui-Fen Hsieh |
| 264 | 1 | |c 1996 | |
| 300 | |a IV, 113 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 500 | |a Heidelberg, Univ., Diss., 1997 (Nicht für den Austausch) | ||
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Datensatz im Suchindex
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CONTENTS
PAGE
1 INTRODUCTION 1
1.1 TRANSCRIPTION FACTORS 1
*
1.1.1 INDUCIBLE TRANSCRIPTION FACTORS (ITFS) 1
1.1.1.1 C-JUN 2
1.1.1.2 JUNB 3
1.1.1.3 JUND 3
1.1.1.4 C-FOS 3
1.1.2 CONSTITUTIVE TRANSCRIPTION FACTORS (CTFS) 4
1.1.2.1 CREB 4
1.1.2.2 ATF-2 5
1.2 FUNCTIONS OF TRANSCRIPTION FACTORS 6
1.3 ELECTROCONVULSIVE SEIZURES (ECS) AND TRANSCRIPTION FACTORS 7
1.4 KAINIC ACID (KA) AND TRANSCRIPTION FACTORS 8
1.5 BRAIN-DERIVED NEUROTROPHIC FACTOR (BDNF) 9
1.5.1 BRAIN-DERIVED NEUROTROPHIC FACTOR AND TRANSCRIPTION FACTORS 11
1.5.2 BRAIN-DERIVED NEUROTROPHIC FACTOR AND ECS 12
1.5.3 BRAIN-DERIVED NEUROTROPHIC FACTOR AND KAINIC ACID (KA) 12
1.6 AIMS FOR THIS STUDY 13
1.6.1 CAN BDNF REGULATE THE EXPRESSION OF ITFS AFTER REPETITIVE ECS? 13
1.6.2 CAN BDNF REGULATE THE EXPRESSION OF TFS AFTER STIMULATION WITH 14
KA IN BRAIN SLICE (BS)?
1.6.3 SETUP OF THE BRAIN SLICE MODEL 15
1.6.4 ISSUES ADDRESSED IN THIS STUDY 16
2 MATERIALS AND METHODS 17
2.1 ELECTROCONVULSIVE SEIZURES 17
I
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/950306819
2.1.1 IMPLANTATION OF MINI-OSMOTIC PUMPS 18
2.1.2 IMMUNOCYTOCHEMISTRY I 19
2.1.3 COUNTING ANALYSIS I 20
2 2 BRAIN SLICE 20
2 2.1 PREPARATION OF SOLUTIONS FOR BRAIN SLICE INCUBATION 20
2.2.2 PREPARATION OF BRAIN SLICES ' 20
2.2.3 IMMUNOCYTOCHEMISTRY II 23
2.2.4 HISTOLOGY 23
2.2.5 COUNTING ANALYSIS II 24
3 RESULTS 25
3.1 ELECTROCONVULSIVE SEIZURES 25
3.1.1 BODY WEIGHT AND SEIZURE ACTIVITIES 25
3.1.2 EXPRESSION OF ITFS 25
3.1.2.1 UNTREATED RATS 25
3.1.2.2 SINGLE ELECTROCONVULSIVE SEIZURES (LXECS) 26
3.1.2.3 5XECS AND LOXECS 27
3.1.3 RE-INDUCTION OF C-FOS 30
3 .1.4 EFFECT OF CHRONIC BDNF INFUSION ON ITFS EXPRESSION FOLLOWING 30
REPETITIVE ECS
3.1.4.1 IMPLANTATION OF THE CANNULA 30
3.1.4.2 INFUSION OF BDNF IN OTHERWISE UNTREATED RATS 30
3.1.4.3 INFUSION OF SALINE (CONTROL) DURING 5XECS AND LOXECS 32
3.1.4.4 INFUSION OF BDNF DURING 5XECS AND LOXECS 32
3.1.4.5 EXPRESSION OF ATF-2 34
3.1.4.6 EXPRESSION OF GFAP 37
3.2 BRAIN SLICE 37
3.2.1 SOLUTIONS PREPARATION FOR BRAIN SLICES INCUBATION 3 7
3.2.2 TEMPERATURE 37
3.2.3 COUNTING STAINING BY CRESYL VIOLET 38
II
3 2.3.1 CORTEX
38
3.2.3.2 HIPPOCAMPUS 42
3.2.4 IMMUNOCYTOCHEMISTRY
44
3.2.4.1 C-FOS
44
3.2.41.1 CORTEX
44
3 2.4.1.2 HIPPOCAMPUS ' 46
3 242 C-JUN
48
3.2.42.1 CORTEX 48
3.2.4.22 HIPPOCAMPUS 50
3.24.3 ATF-2 52
3.24.3.1 CORTEX _ 52
3.2.43
.2 HIPPOCAMPUS 54
3.24.4 CREB
54
3.2.44
.1 CORTEX 54
3.2.44.2 HIPPOCAMPUS
55
3.2.45 PHOPSO-CREB
56
32.4.5.1 CORTEX 56
3.2.45
.2 HIPPOCAMPUS 57
3.2.4
.6 GFAP 57
3.2.4.
6.1 CORTEX 57
3.2.4.62 HIPPOCAMPUS 58
4 DISCUSSION 59
4
.1 IMMUNOCYTOCHEMISTRY 59
4.2 ELECTROCONVULSIVE SEIZURES
59
4.2.1 EXPERIMENTAL MODEL OF ECS
59
4.2 2 SYSTEMIC EFFECTS OF ECS AND BDNF APPLICATION 60
4.2.3 IMPLANTATION OF MINI-OSMOTIC PUMPS 60
4 2.4 DOSAGE OF BDNF 61
4.3 BRAIN SLICE 61
III
4 3 1 EXPERIMENTAL MODEL OF BS IN THE ADULT ANIMAL 62
4.3.2 COMPOSITIONS OF CULTURE MEDIUMS 62
4.3.3 CONDITIONS FOR INCUBATION 63
4.3.3.1 INCUBATION CHAMBER 63
4.3 3.2 TEMPERATURE 63
4.3.3 3 PH VALUE OF MEDIUMS ' 63
4.3.3 4 RISK OF HYPOXIA DURING DISSECTION 64
4.3.3 5 INFUSION RATE 64
4.3.4 DOSAGES OF KA AND BDNF 64
4.4 THE EXPRESSION OF TRANSCRIPTION FACTORS AFTER ECS 65
4.4.1 SINGLE ECS _ 65
4.4.2 DESENSITIZATION OF C-FOS FOLLOWING REPETITIVE ECS 66
4.4.3 THE MEANING OF DOWN-REGULATED EXPRESSION OF C-FOS OR OTHER ITFS 67
4.4.4 RE-INDUCTION OF C-FOS BY BDNF 68
4.4.5 THE TRANSCRIPTIONAL STAND-BYS: ATF-2 AND CREB 70
4.5 THE EXPRESSION OF GFAP AFTER ECS 71
4.6 THE EXPRESSION OF TRANSCRIPTION FACTORS IN THE BS 72
4.6.1 DISTRIBUTION OF NEURONS AND GLIAL CELLS 72
4.6.2 NEURONAL TFS EXPRESSION IN ACSF AND BDNF CULTURES 73
4.6.3 GLIAL TFS EXPRESSION IN ACSF AND BDNF CULTURES 76
4.7 FUNCTIONS OF BDNF IN BS INCUBATION AND KA TREATMENT 79
4.8 CONCLUSIONS 83
5 SUMMARY 85
6 REFERENCES 87
7 CURRICULUM VITAE 111
8 ACKNOWLEDGMENTS 113
IV |
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| indexdate | 2024-10-09T16:09:36Z |
| institution | BVB |
| language | German |
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| physical | IV, 113 S. Ill., graph. Darst. |
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| spelling | Hsieh, Tsui-Fen Verfasser aut Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain vorgelegt von Tsui-Fen Hsieh 1996 IV, 113 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Heidelberg, Univ., Diss., 1997 (Nicht für den Austausch) (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007638831&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Hsieh, Tsui-Fen Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain |
| subject_GND | (DE-588)4113937-9 |
| title | Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain |
| title_auth | Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain |
| title_exact_search | Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain |
| title_full | Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain vorgelegt von Tsui-Fen Hsieh |
| title_fullStr | Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain vorgelegt von Tsui-Fen Hsieh |
| title_full_unstemmed | Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain vorgelegt von Tsui-Fen Hsieh |
| title_short | Modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor (BDNF) in the adult rat brain |
| title_sort | modulation of seizure evoked expression of transcription factors by brain derived neurotrophic factor bdnf in the adult rat brain |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007638831&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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