Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima: its structure, transcription, and a report about the lack of RNA editing
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
1996
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Düsseldorf, Univ., Diss., 1996 |
| Beschreibung: | 85 S. graph. Darst. |
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| 245 | 1 | 0 | |a Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima |b its structure, transcription, and a report about the lack of RNA editing |c vorgelegt von Zhi-Hua Ran |
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| 650 | 4 | |a Meerstrandrübe - Mitochondrium - Plasmid - RNS-Edierung | |
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Datensatz im Suchindex
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| adam_text |
TABLE
OF
CONTENTS
PAGE
1
INTRODUCTION
I
2
MATERIALS
AND
METHODS
4
2.1
MATERIALS
4
2.1.1
CHEMICALS
AND
ENZYMES
4
2.1.2
OLIGONUCLEOTIDE
PRIMERS
4
2.1
3
BUFFERS
5
2.1.4
MEDIA
7
2.1.5
BACTERIAL
STRAINS
7
2.1.6
VECTORS
7
2.1.7
PLANT
MATERIALS
7
2.2
METHODS
8
2.2.1
ISOLATION
OF
LINEAR
PLASMID
PBM
DNA
8
2.2.1.1
ISOLATION
OF
MITOCHONDRIAL
DNA
FROM
LEAVES
OF
WILD
BEETS
8
2.2
1.2
PURIFICATION
OF
MITOCHONDRIAL
DNA
BY
CSCL-ETBR
DENSITY
GRADIENT
CENTRIFUGATION
9
2.2
1.3
SEPARATION
OF
THE
LINEAR
PLASMID
PBM
FROM
MITOCHONDRIAL
DNA
10
2
2.2
RESTRICTION
OF
DNA
10
2
2.3
AGAROSE
GEL
ELECTROPHORESIS
OF
DNA
10
2
2.4
ELUTION
OF
DNA
FROM
AGAROSE
GELS
10
2.2.5
CLONING
OF
DNA
11
2.2.5.1
CLONING
OF
RESTRICTION
FRAGMENTS
OF
PBM
11
22.5.2
TAILING
OF
THE
TERMINAL
FRAGMENTS
OF
PBM
11
2.2.5.3
CLONING
OF
CDNA
IN
THE
T-VECTOR
11
2.2.5.4
LIGATION
12
2.2.5.5
TRANSFORMATION
OF
E.
COLI
12
2.2.6
ISOLATION
OF
PLASMID
DNA
FROM
COLI
13
2.2.7
IDENTIFICATION
OF
RECOMBINANT
CLONES
13
2.2.8
EXONUCLEASE
III
DIGESTION
OF
DNA
13
2
2.9
SEQUENCE
ANALYSIS
OF
DNA
14
2.2
10
SOUTHERN
HYBRIDIZATION
15
2.2.10.1
TRANSFER
OF
RESTRICTION
FRAGMENTS
TO
MEMBRANES
15
2.2.10.2
LABELING
OF
DNA
15
2.2.10.3
RADIOACTIVE
HYBRIDIZATION
16
2.2.10
4
NONRADIOACTIVE
HYBRIDIZATION
16
2.2.11
COLONY
HYBRIDIZATION
16
2.2.12
ISOLATION
OF
TOTAL
RNA
FROM
BETA
VULGARIS
AND
BETA
MARITIMA
17
2.2.13
AGAROSE
GEL
ELECTROPHORESIS
OF
RNA
17
2.2
14
NORTHERN
HYBRIDIZATION
18
2.2.15
RT-PCR
AMPLIFICATION
OF
RNA
18
2.2.15.1
DNASE
I
TREATMENT
OF
RNA
18
2.2.15.2
REVERSE
TRANSCRIPTION
OF
RNA
18
2.2.15.3
PCR
AMPLIFICATION
19
2.2.16
COMPUTER
ANALYSIS
19
3
RESULTS
AND
DISCUSSION
20
3.1
CLONING
OF
INTERNAL
AND
TERMINAL
FRAGMENTS
OF
PBM
20
3.2
SEQUENCING
STRATEGY
22
3.3
GENOME
ORGANIZATION
OF
PBM
25
3.4
0RF1
AND
ORF2
OFPBM
38
3.5
PBM
ENCODES
A
B-TYPE
DNA
POLYMERASE
40
3.6
PBM
ENCODES
A
VIRUS-LIKE
RNA
POLYMERASE
47
3.7
TERMINAL
INVERTED
REPEATS
OF
PBM
54
3.8
TRANSCRIPT
ANALYSIS
OF
PBM
59
3.9
SEARCH
FOR
EDITING
OF
PBM
TRANSCRIPTS
65
4
CONCLUSION
AND
OUTLOOK
71
5
ABSTRACT
72
6
ZUSAMMENFASSUNG
73
7
REFERENCES
74 |
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| author_facet | Ran, Zhi-Hua |
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| building | Verbundindex |
| bvnumber | BV011332001 |
| ctrlnum | (OCoLC)258236725 (DE-599)BVBBV011332001 |
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| id | DE-604.BV011332001 |
| illustrated | Illustrated |
| indexdate | 2024-08-17T00:56:42Z |
| institution | BVB |
| language | German |
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| oclc_num | 258236725 |
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| owner_facet | DE-29T DE-11 |
| physical | 85 S. graph. Darst. |
| publishDate | 1996 |
| publishDateSearch | 1996 |
| publishDateSort | 1996 |
| record_format | marc |
| spelling | Ran, Zhi-Hua Verfasser aut Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima its structure, transcription, and a report about the lack of RNA editing vorgelegt von Zhi-Hua Ran 1996 85 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Düsseldorf, Univ., Diss., 1996 Meerstrandrübe - Mitochondrium - Plasmid - RNS-Edierung Meerstrandrübe - Mitochondrium - Plasmid - Transkription <Genetik> Plasmid (DE-588)4046270-5 gnd rswk-swf Mitochondrium (DE-588)4124939-2 gnd rswk-swf RNS-Edierung (DE-588)4350250-7 gnd rswk-swf Transkription Genetik (DE-588)4185906-6 gnd rswk-swf Meerstrandrübe (DE-588)4334570-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Meerstrandrübe (DE-588)4334570-0 s Mitochondrium (DE-588)4124939-2 s Plasmid (DE-588)4046270-5 s Transkription Genetik (DE-588)4185906-6 s DE-604 RNS-Edierung (DE-588)4350250-7 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007613980&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ran, Zhi-Hua Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima its structure, transcription, and a report about the lack of RNA editing Meerstrandrübe - Mitochondrium - Plasmid - RNS-Edierung Meerstrandrübe - Mitochondrium - Plasmid - Transkription <Genetik> Plasmid (DE-588)4046270-5 gnd Mitochondrium (DE-588)4124939-2 gnd RNS-Edierung (DE-588)4350250-7 gnd Transkription Genetik (DE-588)4185906-6 gnd Meerstrandrübe (DE-588)4334570-0 gnd |
| subject_GND | (DE-588)4046270-5 (DE-588)4124939-2 (DE-588)4350250-7 (DE-588)4185906-6 (DE-588)4334570-0 (DE-588)4113937-9 |
| title | Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima its structure, transcription, and a report about the lack of RNA editing |
| title_auth | Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima its structure, transcription, and a report about the lack of RNA editing |
| title_exact_search | Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima its structure, transcription, and a report about the lack of RNA editing |
| title_full | Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima its structure, transcription, and a report about the lack of RNA editing vorgelegt von Zhi-Hua Ran |
| title_fullStr | Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima its structure, transcription, and a report about the lack of RNA editing vorgelegt von Zhi-Hua Ran |
| title_full_unstemmed | Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima its structure, transcription, and a report about the lack of RNA editing vorgelegt von Zhi-Hua Ran |
| title_short | Molecular analysis of the linear mitochondrial plasmid pBM of Beta maritima |
| title_sort | molecular analysis of the linear mitochondrial plasmid pbm of beta maritima its structure transcription and a report about the lack of rna editing |
| title_sub | its structure, transcription, and a report about the lack of RNA editing |
| topic | Meerstrandrübe - Mitochondrium - Plasmid - RNS-Edierung Meerstrandrübe - Mitochondrium - Plasmid - Transkription <Genetik> Plasmid (DE-588)4046270-5 gnd Mitochondrium (DE-588)4124939-2 gnd RNS-Edierung (DE-588)4350250-7 gnd Transkription Genetik (DE-588)4185906-6 gnd Meerstrandrübe (DE-588)4334570-0 gnd |
| topic_facet | Meerstrandrübe - Mitochondrium - Plasmid - RNS-Edierung Meerstrandrübe - Mitochondrium - Plasmid - Transkription <Genetik> Plasmid Mitochondrium RNS-Edierung Transkription Genetik Meerstrandrübe Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007613980&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ranzhihua molecularanalysisofthelinearmitochondrialplasmidpbmofbetamaritimaitsstructuretranscriptionandareportaboutthelackofrnaediting |