A laboratory guide to glycoconjugate analysis:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | German |
| Veröffentlicht: |
Basel ; Boston ; Berlin
Birkhäuser
1997
|
| Schriftenreihe: | Biomethods
9 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXII, 405 S. Ill., graph. Darst. |
| ISBN: | 3764352108 0817652108 |
Internformat
MARC
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| 245 | 1 | 0 | |a A laboratory guide to glycoconjugate analysis |c ed. by P. Jackson ... |
| 264 | 1 | |a Basel ; Boston ; Berlin |b Birkhäuser |c 1997 | |
| 300 | |a XXII, 405 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 490 | 1 | |a Biomethods |v 9 | |
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| 650 | 4 | |a Glycoconjugates |x analysis |v Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804125715861340160 |
|---|---|
| adam_text | ^^^H Contents
List of Contributors XIII
Preface XVII
Abbreviations XIX
BHigh Performance Liquid Chromatography of Derivatized
and Non Derivatized Oligosaccharides: A Review 1
1.1 Introduction 1
1.2 Separation Techniques 1
Size exclusion chromatography 2
Ion exchange chromatography 5
Hydrophilic interaction chromatography 7
Reversed phase chromatography 11
Mixed elution protocols 14
1.3 Detection Techniques 15
Refractometer 15
UV detector 15
Fluorescence and photometric detection after derivatization 16
Detection after Postcolumn Reaction 17
Miscellaneous Detection Techniques 19
References 20
EM Detection of the Presence of Carbohydrates in Glycoproteins 23
2.1 Introduction 23
A general chemical based method for glycoprotein identification 24
2.2 Principle 25
Substances interfering with detection 25
2.3 Glycoprotein Detection on Membranes or in Solution:
Relative Merits of Protocols 26
Membrane labelling 26
Solution labelling 26
Sensitivity of detection 26
Initial experiments 26
Interpretation of results and troubleshooting 29
Additional analyses: Alternative detection techniques 31
2.4 Identification of Specific Monosaccharides or Glycans 31
Lectins 31
Monoclonal antibodies to carbohydrate determinants 32
Glycosyl transferases 32
Naturally occurring carbohydrate recognition proteins 32
Glycosyl phosphatidyl inositol anchor detection 33
2.5 Detailed Analysis of Protein Glycosylation Release Label Profile 35
Acknowlegements 38
References 38
aHPAE PAD Monosaccharide and Oligosaccharide Analysis of Glycoproteins
Electrotransferred onto Polyvinylidene Fluoride Membranes 41
3.1 Introduction 41
3.2 SDS PAGE 43
3.3 Electroblotting and Staining 44
3.4 Monosaccharide Composition Analysis 47
Chromatography, detection and quantification of monosaccharides 53
3.5 Oligosaccharide Mapping 54
Acknowledgement 58
References 58
a Determination of the Disaccharide Composition of Glycosaminoglycans:
Comparison of Chemical and Enzymatic Scission 61
4.1 Introduction 61
4.2 Technical Procedures 65
Sample preparation 67
4.3 Troubleshooting 74
Enzyme digests 74
Chemical depolymerization 75
HPLC analysis 75
Acknowledgements 76
References 76
£M Mass Spectrometric Analysis of Highly Acidic Polysaccharides 77
5.1 Introduction 77
5.2 Principles of Matrix Assisted Laser Desorption 77
5.3 Technical Procedures 78
Sample preparation 79
5.4 Troubleshooting 84
References 89
¦ Analysis of N Glycans by Matrix Assisted Laser Desorption/lonization
Mass Spectrometry 91
6.1 Introduction 91
6.2 MALDI MS: Principle of Operation 92
Instrumentation 94
Analytical protocols 94
6.3 Analysis of Glycoproteins 95
Glycoprotein De N glycosylation with PNGase F 96
MALDI MS of glycoproteins 98
6.4 Analysis of Glycopeptides 99
Structural analysis of N glycans by MALDI MS of glycopeptides in combination
with exoglycosidase array sequencing 100
MALDI MS of glycopeptides 105
6.5 Analysis of Released Oligosaccharides 105
Analysis of released N glycans by MALDI MS 107
MALDI MS of glycans 108
6.6 Analysing Carbohydrate Mass Information 109
Quantitation 109
Accessing the complex carbohydrate structure database 110
Data for calculation of oligosaccharide masses 110
References 111
aPolyacrylamide Gel Electrophoresis of Fluorophore Labelled Reducing
Saccharides: A Review 113
7.1 Introduction 113
7.2 Principle 114
7.3 Preparation of Wheat Starch Digest Electrophoretic Standard 122
Troubleshooting 123
7.4 Enzymatic Release of Asparagine Linked Oligosaccharides from
Glycoproteins Using PNGase F 123
Troubleshooting 126
7.5 Release of Asparagine and Serine/Threonine Linked Glycans from
Glycoproteins Using Hydrazinolysis 126
7.6 Derivatization of the Oligosaccharides with ANTS 128
7.7 Derivatization of Oligosaccharides with AMAC 129
7.8 PAGE for the Separation of ANTS Derivatized Oligosaccharides and
for Acidic Oligosaccharides Derivatized with AMAC 130
Troubleshooting 133
7.9 PAGE for the Separation of Neutral and Acidic AMAC Derivatized Saccharides 133
7.10 Enzymatic Structural Analysis of N Glycans 135
7.11 Viewing and Imaging the Electrofluorograms 137
References 139
£M Carbohydrate Analysis with Capillary Electrophoresis 141
8.1 Introduction 141
Instrumentation 142
Capillaries 142
8.2 Sample Preparation 144
Glycoprotein and oligosaccharide hydrolysis for compositional analysis 144
Release of complex oligosaccharides 144
Enzymatic cleavage 147
Derivatization 148
8.3 Capillary Electrophoretic Separations 151
Monosaccharides 151
Complex oligosaccharides 153
Glycoproteins 156
References 159
gB| Oligosaccharide Profiling of Keratan Sulphate 161
9.1 Introduction 161
9.2 Technical Procedures 163
Keratan sulphate preparation from tissues 165
KS molecular weight determination 166
Oligosaccharide profiling 168
Hydrazinolysis/nitrous acid 168
Keratanase (EC 3.2.1.103) 171
Keratanase II (EC 3.2.1. from Bacillussp.) 172
Chromatography 174
Borotriti ide reduction and [35S] labelled samples 180
Acknowledgements 180
References 181
H Analysis of the Structure of Heparin and Heparan Sulfate by High Resolution
Separation of Oligosaccharides 183
10.1 Introduction 183
10.2 Technical Procedures 185
10.3 Method Selection, Critical Parameters and Troubleshooting 195
Anticipated results 196
Time considerations 196
References 197
HI HPLC Strategies for Profiling and Sequencing Oligosaccharides 199
11.1 Introduction 199
Oligosaccharide profiling 200
Detailed structural analysis 200
11.2 HPLC Separations Technology 201
Normal phase HPLC 201
Choice of column 201
Optimization of solvents and gradients 201
Profiling and sequencing 202
Weak anion exchange HPLC 203
Reversed phase HPLC 204
11.3 Requirements of HPLC System for Glycan Analysis 207
HPLC hardware 207
Buffer systems 207
Sensitivity 207
Reproducibility 208
Predictive values 208
11.4 Technical Procedures 209
Procedures required to calibrate normal phase HPLC columns 214
Notes 221
Reversed phase HPLC: a second dimension technique to resolve peaks
which overlap on normal phase: e.g. oligosacharides with and without
bisecting GlcNAc 232
Acknowledgements 233
References 234
|fj Enzymatic Sequence Analysis of Glycoprotein Glycans 235
12.1 Introduction 235
Detection methods 235
Sequential sequencing 237
Interpretation of results 238
Reagent array analysis method (RAAM) 238
Designing an enzyme array 239
Sialylated N glycans 241
12.2 Technical Procedures 243
Glycan release 245
Glycan labelling 245
Glycan separation 245
Separation of charged glycans on GlycoSep C 246
Further purification of fractions on GlycoSep H 247
Desialylation procedure (sialylated glycan array only) 249
Sequencing of trisialylated glycans from bovine serum fetuin 249
Data analysis 252
Additional information 254
Limitations 254
Alternative analytical methods 255
12.3 Troubleshooting Guide 256
Sample contains 15% of another glycan species 256
Insufficient sample ( 100 pmol) 256
Salts remain in the sample after RAAM 257
Enzyme action not completely stopped before repooling 257
Unsuitable samples 257
Use of a control 257
Note 258
References 259
HExosequencing of Heparan Sulphate/Heparin Saccharides Using
Lysosomal Enzymes 261
13.1 Introduction 261
13.2 Derivatization of GAG Saccharides With the Fluorophores AMCAH and 2 AA . 265
AMCAH coupling 266
Notes 267
13.3 Treatment of GAG Saccharides With Lysosomal Exoenzymes 267
13.4 PAGE Separation of Saccharides 270
13.5 Imaging the Gels 273
13.6 Electrotransfer and Recovery of Resolved Saccharides 274
Acknowledgements 276
References 277
HThe Application of Two Dimensional Capillary Electrophoresis to the
Identification of Glycan Structures 279
14.1 Introduction 279
14.2 Outlines of the Technique 280
14.3 Release of Glycans by Hydrazinolysis 280
14.4 Derivatizing the Released Glycans with PA 282
14.5 Analysis and Confirmation of PA Glycans by 2D CE Using
Fluorimetric Detection 284
14.6 Troubleshooting Guide 292
References 293
HThe Application of Three Dimensional HPLC to the Identification of
N linked Oligosaccharide Structures 295
15.1 Introduction 295
15.2 Isolation and Identification of Neutral and Sialyl PA Oligosaccharides
by Three Successive HPLC Columns 295
15.3 Technical Procedures 297
Differentiation between Neu5Ac a (2,6) and Neu5Ac a (2,3) containing
oligosaccharides 302
Differentiation between Neu5Gc and Neu5Ac containing oligosaccharides .... 303
15.4 Notes 304
References 306
H Simultaneous Fluorescent Labelling and Biotinylation of Oligosaccharides:
A Versatile Approach to the Analysis of Oligosaccharide
Structure and Function 307
16.1 Introduction 307
16.2 Synthesis and Purification of BAP 310
Troubleshooting guide 313
16.3 Coupling of Oligosaccharides to BAP 314
Troubleshooting guide 315
16.4 Purification of BAP Oligosaccharides 316
Troubleshooting guide 319
16.5 Fractionation and Structural Analysis of BAP Adducts 320
Troubleshooting guide 325
16.6 Preparation of BAP Oligosaccharide Neoglycoconjugates With Streptavidin
or Avidin and Their Applications 325
Troubleshooting guide 326
16.7 Comparison of BAP With Other Currently Available Fluorescent Tags 327
References 328
H Preparation of Neoglycolipids for Structure and Function
Assignments of Oligosaccharides 329
17.1 Introduction 329
17.2 Preparation of Neoglycolipids (Conjugation of Oligosaccharides) 332
17.3 Separation and Purification of Neoglycolipids 335
17.4 Visualization and Quantitation of Neoglycolipids 341
Densitometry 344
17.5 Chemical Analysis of Neoglycolipids 344
Characterization of neoglycolipids by MS 344
MS 345
17.6 Enzymatic and Chemical Modifications of Neoglycolipids 345
17.7 Troubleshooting 347
References 348
|f | Analysis of Protein Carbohydrate Interactions by FACE/Gel
HJi Retardation Assay 349
18.1 Introduction 349
18.2 Principle of FACE/Gel Retardation Assay 349
18.3 Labelling of Oligosaccharides with ANTS 350
18.4 Screening of Carbohydrate Ligands for Proteins 352
18.5 Measurement of Binding Constant for the Interaction Between Protein
and ANTS Labelled Carbohydrate 355
18.6 Measurement of Binding Constant for the Interaction Between Protein and
Native Carbohydrate 357
References 360
HThe Application of Capillary Affinity Electrophoresis to the Analysis
of Carbohydrate Protein Interactions 361
19.1 Introduction 361
19.2 Principle of CAE 363
19.3 Determination of Association Constants 364
19.4 Technical Procedures 366
General considerations 366
19.5 Limitations of the Technique 370
19.6 Application of CAE to the Analysis of Carbohydrate Protein Interactions 371
19.7 Conclusions 375
References 377
EJjj Analysis of Protein Glycosaminoglycan Interactions by Affinity
¦¦ Co Electrophoresis 379
20.1 Introduction 379
Definitions 380
20.2 Technical Procedures 381
20.3 Sample Detection and Sample Recovery 389
Autoradiography and staining 389
Sample detection by blotting 389
Semipreparative ACE 390
20.4 Analysis of Data 391
Measuring sample mobilities calculating a retardation coefficient 391
Graphical analysis of data 392
Interpreting ACE patterns 393
Reverse ACE 395
20.5 Summary 397
Acknowledgements 398
References 398
Subject Index 399
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| id | DE-604.BV011217517 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:05:58Z |
| institution | BVB |
| isbn | 3764352108 0817652108 |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007525690 |
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| physical | XXII, 405 S. Ill., graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
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| publisher | Birkhäuser |
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| series | Biomethods |
| series2 | Biomethods |
| spelling | A laboratory guide to glycoconjugate analysis ed. by P. Jackson ... Basel ; Boston ; Berlin Birkhäuser 1997 XXII, 405 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biomethods 9 Glycoconjugates Analysis Laboratory manuals Glycoconjugates analysis Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Glykokonjugate (DE-588)4131221-1 gnd rswk-swf Glykokonjugate (DE-588)4131221-1 s Biochemische Analyse (DE-588)4255721-5 s DE-604 Jackson, Peter Sonstige oth Biomethods 9 (DE-604)BV002454363 9 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007525690&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | A laboratory guide to glycoconjugate analysis Biomethods Glycoconjugates Analysis Laboratory manuals Glycoconjugates analysis Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd Glykokonjugate (DE-588)4131221-1 gnd |
| subject_GND | (DE-588)4255721-5 (DE-588)4131221-1 |
| title | A laboratory guide to glycoconjugate analysis |
| title_auth | A laboratory guide to glycoconjugate analysis |
| title_exact_search | A laboratory guide to glycoconjugate analysis |
| title_full | A laboratory guide to glycoconjugate analysis ed. by P. Jackson ... |
| title_fullStr | A laboratory guide to glycoconjugate analysis ed. by P. Jackson ... |
| title_full_unstemmed | A laboratory guide to glycoconjugate analysis ed. by P. Jackson ... |
| title_short | A laboratory guide to glycoconjugate analysis |
| title_sort | a laboratory guide to glycoconjugate analysis |
| topic | Glycoconjugates Analysis Laboratory manuals Glycoconjugates analysis Laboratory Manuals Biochemische Analyse (DE-588)4255721-5 gnd Glykokonjugate (DE-588)4131221-1 gnd |
| topic_facet | Glycoconjugates Analysis Laboratory manuals Glycoconjugates analysis Laboratory Manuals Biochemische Analyse Glykokonjugate |
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