Neuropeptide protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1997
|
| Schriftenreihe: | Methods in molecular biology
73 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIII, 386 S. graph. Darst. |
| ISBN: | 0896033996 |
Internformat
MARC
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| 245 | 1 | 0 | |a Neuropeptide protocols |c ed. by G. Brent Irvine ... |
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| 300 | |a XIII, 386 S. |b graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804125662571659264 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
1 Preparation of Neuropeptide Containing Fractions from Biological
Materials,
J. Michael Conlon 1
2 Purification of Extracted Peptides for Structural Analysis,
Chris Shaw 9
3 Amino Acid Sequencing of Neuropeptides,
Ka Wan Li and Wijnand P. M. Geraerts 17
4 Neuropeptide Gene Identification Using the Polymerase Chain
Reaction,
Aaron G. Maule and Timothy G. Geary 27
5 Solid Phase Synthesis of Neuropeptides by Fmoc Strategies,
Chris Kowalczyk and Michael O Shea 41
6 Incorporation of Stable Pseudopeptide Bonds: Methylene Amino,
Thioether, and Hydroxyethylene Derivatives,
Graeme J. Anderson 49
7 Synthesis of Conformational^ Restricted Peptides,
Annette G. Beck Sickinger 61
8 Purification of Synthetic Peptides by High Performance Liquid
Chromatography,
D. David Smith and Ann M. Hanly 75
9 Molecular Weight Estimation for Neuropeptides Using Size Exclusion
High Performance Liquid Chromatography,
G. Brent Irvine 89
10 Molecular Weight Determinations Using Polyacrylamide
Gel Electrophoresis in Tris Tricene Buffers,
G. Brian Wisdom 97
11 Determination of Neuropeptides by Capillary Electrophoresis,
SungAe S. Park, Wei Lun Hung, Daniel E. Schaufelberger,
Norberto A. Guzman, and Juan P. Advis 101
vii
via Contents
12 Characterization of Neuropeptide Processing by Fast Atom
Bombardment Mass Spectrometry,
Jerzy Silberring 113
13 Analysis of Neuropeptides by Size Exclusion HPLC Linked to
Electrospray Ionization Mass Spectrometry,
Jerzy Silberring 129
14 Identification of Peptides by Matrix Assisted Laser Desorption
Ionization Time of Flight Mass Spectrometry (MALDI TOF MS)
and Direct Analysis of the Laterobuccal Nerve from the Pond Snail
Lymnaea stagnalis,
Glenn Critchley and Belinda Worster 141
15 Use of Circular Dichroism to Determine Secondary Structure
of Neuropeptides,
Laszlo Otvos, Jr. 153
16 H Nuclear Magnetic Resonance (NMR) in the Elucidation of Peptide
Structure,
David J. S. Guthrie 163
17 The Study of Membrane or Receptor Bound Neuropeptides
by NMR,
Rickey P. Hicks 185
18 Molecular Modeling of Neuropeptides,
Sandor Lovas and Richard F. Murphy 209
19 Tritium Labeling of Neuropeptides,
Geza Toth, Sandor Lovas, and Ferenc Otvos 219
20 The Use of IODO GEN for Preparing 125l Labeled Peptides
and Their Purification by Reversed Phase High Performance
Liquid Chromatography,
J. Michael Conlon 231
21 Production of Antisera Using Peptide Conjugates,
Thomas E. Adrian 239
22 Radioimmunoassay,
Thomas E. Adrian 251
23 Enzyme Linked Immunosorbent Assay of Peptides,
Laszlo Otvos, Jr. and Gyorgyi I. Szendrei 269
24 Sample Preparation for Peptide Immunocytochemistry,
KathyM. Pogue and Colin F. Johnston 277
25 Immunocytochemical Methods for Regulatory Peptides,
KathyM. Pogue and Colin F. Johnston 283
Contents ix
26 Ultrastructural Localization of Peptides Using Immunogold Labeling,
David W. Halton and Gerard P. Brennan 293
27 Preparation of a Membrane Fraction for Receptor Studies and
Solubilization of Receptor Proteins with Retention of Biological
Activity,
Mark Wheatley, John Howl, Nicola J. Yarwood,
Andrew R. L. Davies, and Rosemary A. Parslow 305
28 Radioligand Binding Using i25l Labeled Peptides,
Peter W. Abel, David Waugh, and William B. Jeffries 323
29 Analysis of Data from Cold Saturation Radioligand Binding
Experiments,
William B. Jeffries, David Waugh, and Peter W. Abel 331
30 Organ/Tissue Preparation for the Assessment of Agonist/
Antagonist Activity,
Mark Murnin, Sandor Lovas, James M. Allen,
and Richard F. Murphy 343
31 Measurement of Efflux Rates from Brain to Blood,
William A. Banks, Melita B. Fasold, and Abba J. Kastin 353
32 Assays of Neuropeptidases Using Fluorogenic Substrates,
CarvellH. Williams 361
33 Characterization of Neuropeptides Using Inhibitors,
Nigel M. Hooper 369
Index 383
|
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV011168622 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:05:07Z |
| institution | BVB |
| isbn | 0896033996 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007487378 |
| oclc_num | 231693630 |
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| owner | DE-12 DE-29T DE-703 DE-578 |
| owner_facet | DE-12 DE-29T DE-703 DE-578 |
| physical | XIII, 386 S. graph. Darst. |
| publishDate | 1997 |
| publishDateSearch | 1997 |
| publishDateSort | 1997 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Neuropeptide protocols ed. by G. Brent Irvine ... Totowa, NJ Humana Press 1997 XIII, 386 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 73 Chemische Analyse (DE-588)4009840-0 gnd rswk-swf Peptidsynthese (DE-588)4173715-5 gnd rswk-swf Neuropeptide (DE-588)4041895-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Neuropeptide (DE-588)4041895-9 s Chemische Analyse (DE-588)4009840-0 s DE-604 Peptidsynthese (DE-588)4173715-5 s Irvine, G. Brent Sonstige oth Methods in molecular biology 73 (DE-604)BV035362695 73 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007487378&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Neuropeptide protocols Methods in molecular biology Chemische Analyse (DE-588)4009840-0 gnd Peptidsynthese (DE-588)4173715-5 gnd Neuropeptide (DE-588)4041895-9 gnd |
| subject_GND | (DE-588)4009840-0 (DE-588)4173715-5 (DE-588)4041895-9 (DE-588)4143413-4 |
| title | Neuropeptide protocols |
| title_auth | Neuropeptide protocols |
| title_exact_search | Neuropeptide protocols |
| title_full | Neuropeptide protocols ed. by G. Brent Irvine ... |
| title_fullStr | Neuropeptide protocols ed. by G. Brent Irvine ... |
| title_full_unstemmed | Neuropeptide protocols ed. by G. Brent Irvine ... |
| title_short | Neuropeptide protocols |
| title_sort | neuropeptide protocols |
| topic | Chemische Analyse (DE-588)4009840-0 gnd Peptidsynthese (DE-588)4173715-5 gnd Neuropeptide (DE-588)4041895-9 gnd |
| topic_facet | Chemische Analyse Peptidsynthese Neuropeptide Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007487378&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT irvinegbrent neuropeptideprotocols |