Verfügbarkeit: Enzyme engineering

Enzyme engineering: 13

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Format:	Tagungsbericht Buch
Sprache:	English
Veröffentlicht:	New York, NY 1996
Schriftenreihe:	New York Academy of Sciences: Annals of the New York Academy of Sciences 799
Schlagworte:	Encimi - Biotehnologija - Posvetovanja Konferenzschrift
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XV, 790 S. Ill., zahlr. graph. Darst.
ISBN:	0897669819 0897669827

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adam_text	ANNALS OF THE NEW YORK ACADEMY OF SCIENCES Volume 799 October 12,1996 ENZYME ENGINEERING XIIIй Editors and Conference Organizers Jonathan S. Dordick and Alan J. Russell ____________________________ CONTENTS ____________________________ Introduction. By Jonathan S. Dordick and Alan J. Russell ........................ xiii The Enzyme Engineering Award .......................................................................... xv Part I. Preparation and Properties of Designed Biocatalysts Strategy for the Directed Evolution of a Peptide Ligase. By HuiMiN Zhao, You Li, and Frances H. Arnold ................................................................. 1 Towards Engineering an Improved Morphine Dehydrogenase. By Edward H. Walker and Neil С Bruce ..................................................................... 6 Production and Modification of E. coli Transketolase for Large-Scale Biocatalysis. By Carol French and John M. Ward ................................... 11 Transglycosylation by Wild Type and Mutants of a ß-l,4-Glycosidase from Cellulomonas fimi (Cex) for Synthesis of Oligosaccharides. By P. V. Nikolova, S. Duff, A. MacLeod, and C. A. Haynes ................................ 19 Catalytic Antibodies: The Rerouting of Chemical Reactions — Towards Electrophilic Aromatic Substitution by Carbon Dioxide. By Jari T. Yli-Kauhaluoma and Kim D. Janda .......................................................... 26 Engineering of Novel Polyketides: Progress and Prospects. By Peter J. Kramer and Chaitan Khosla ..................................................................... 32 Characterization of Polyclonal Antibody-catalyzed Hydrolysis. By Dongzhou Liu and Yaoting Yu ................................................................ 46 Preparation of Bifunctional Enzyme with Both Superoxide Dismutase and Glutathione Peroxidase Activities by Using Chemical Mutation. By Guimin Luo, Weihua Liu, Qian Sun, Lan Ding, Zhenqi Zhu, Ganglin Yan, and Tongshu Yang ............................................................. 50 Control of the Catalytic Mechanism of an Enzyme by Amino Acid Substitution. By Keiichi Morimoto, Ryota Kuroki, and Brian W. Matthews ....................................................................................................... 56 An Engineered Penicillin Acylase with Altered Surface Charge Is More Stable in Alkaline pH. By Gabriel del Río, Agustín López-Munguía, and Xavier Soberón ............................................................................................ 61 Modification of Alpha-Amylase Functions by Protein Engineering. By Masaaki Terashima and Shigeo Katoh .................................................... 65 β η This volume is the result of a conference entitled the Thirteenth International Enzyme Engineering Conference, which was sponsored by the Engineering Foundation, New York, and held on October 15-20,1995, in San Diego, California. Site-directed Mutagenesis of Cysteinyl Residues in Aspartase oiEscherìchia coli. By Нѕш -Hui Chen, Jin-Tann Chen, and Hsin Tsai ............................ 70 Protein Engineering of a Cephalosporin С Acylase. By Hisashi Yamada, Yoshinori Ishii, Уші Noguchi, Toshiko Miura, Thoru Mori, and Yoshimasa Saito ........................................................................................... 74 Thermal Stable Subtilisin with Oxidation-resistant Property. By Liuqin Zhu, Yufeng Zhao, and Ruchang Bi .................................................................. 82 Part II. Biopolymer Structure and Function The Structural Effects of Solvent Engineering. By Gregory K. Färber......... 85 The Use of a Novel Recombinant Heroin Esterase in the Development of an Illicit Drugs Biosensor. By Deborah A. Rathbone, Peter-John Holt, Christopher R. Lowe, and Neil С Bruce ................................................ 90 Morphinone Reducíase: Characterization, Cloning, and Application to Biocatalytic Hydromorphone Production. By C. E. French, A. M. Hailes, D. A. Rathbone, and N. C. Bruce ................................................. 97 New Approaches for the Verification of Kinetic Parameters of Immobilized Concanavalin A:Invertase Preparations Investigated by Flow Microcalorimetry. By Pjeter Gemeiner, Peter Dočolomanský, Jozef Nahálka, Vladimír Štefuca, and Bengt Danielsson ............................ 102 The Effect of Water Content on the Thermostability of Solid-State Proteins. By Guido Greco, Jr., Domenico Pirozzi, Giuseppe Toscano, and Michele Maremonti .................................................................................... 108 The Use of Rational Mutagenesis to Modify the Chain Length Specificity of a Rhizopus délemar Lipase. By Michael J. Haas, Rolf D. Joerger, Gregory King, and Robert R. Klein ........................................................ 115 Production, Characterization, and Molecular Modeling of Lipases for Esterification. By Sanna Jääskeläinen, Xiao Yan Wu, Susan Linko, Yongxiang Wang, Yu-Yen Linko, Olle Teleman, and Pekka Linko.. 129 Complicated Simplification of Dosage Regimen for Plasminogen Activators. By Alexander V. Maksimenko ................................................................... 139 Effective Enzyme Thrombolytic Composition on the Basis of Wild-type and Chemically Modified Plasminogen Activators. By Alexander V. Maksimenko, Elena G. Tischenko, Artem D. Petrov, and Marina L. Petrova ...................................................................................................... 146 Primary Structure of Dextransucrase from Leuconostoc mesenteroides NRRL B-1299: Comparison with Dextransucrase from Leuconostoc mesenteroides NRRL B-512F. By Vincent Monchois, Rene-Marc Willemot, Magali Remaud-Simeon, Christian Croux, Philippe Soucaille, and Pierre Monsan .............................................................................................. 151 Parameters Affecting Lipase Production by HEMA-immobilized Bacteria. By C. N. A. Razak, Z. Zakaria, A. B. Salleh, K. Ampon, W. M. Z. Yunus, M. Basri, and D. Mohd ................................................................... 157 Automated Docking of Glucoamylase Substrates and Inhibitors. By Pedro M. Coutinho, Michael K. Dowd, and Peter J. Reilly ........................... 164 Polyclonal Catalytic Anti-idiotypic Antibodies with a β -Lactamase Activity. By Berangere Avalle, Dominique Mistro, Daniel Thomas, and Alain Friboulet ........................................................................................... 172 Localization of an Endoglucanase and a Xylanase from Aspergillus niger in Soybean by Immunogold-Cytochemical Labeling. By T. Varzakas, D. L. Pyle, and K. Niranjan .................................................................................. 176 Effect of Monovalent Cations (K+ and Na*) on Lactose Hydrolysis by Kluyveromyces lactis ß-Galactosidase: A Kinetic Model. By María V. Flores, Rodolfo J. J. Ertola, and Claudio E. Voget .......................... 183 Structure-Function Studies on the Chitinolytic Enzymes of Serratici marcescens Chitinase and Chitobiase. By C. E. Vorgias, A. Perrakis, and I. Tews ...................................................................................................... 190 Effect of N-Linked Oligosaccharide on the Conformation and Properties of Glucoamylase from Monascus rubiginosus. By Zhi Yang Dong, Shou Jun Yang, Cheng Jin, and Shu Zheng Zhang .......................................... 193 Part III. Biocatalysts under Extreme Environments Immobilization of Enzymes for Use in Organic Media. By PATRICK Adlercreutz, Raúl Barros, and Ernst Wehtje .................................... 197 Strategies for Enhancing Enzymatic Properties in Organic Solvent. By Shu-Gui Cao, Hong Yang, Huan Dong, Nian-Xiang Zhang, Si-Ping Han, Shi-De Liu, Zhan-Bin Liu, and Tong-Su Yang ............................... 201 Enzymatic Catalysis in Nonconventional Media Using High Polar Molecules as Substrates. By Edmundo Castillo, Alain Marty, J. Stephane Condoret, and Didier Combes ................................................................... 206 High Pressure and Temperature: How to Deactivate Enzymes in Two Different Ways. By Dominique Cavaille and Didier Combes ................. 212 Effects of Hydrostatic Pressure on Catalytic Activity and Stability of Two Alcohol Dehydrogenases. By Sandrine Dallet and Marie-Dominique Legoy .............................................................................................................. 218 Controlling Regioselectivity in Enzyme-catalyzed Acylation of Polyhydroxyl Compounds. By Joseph O. Rich and Jonathan S. Dordick ..................... 226 Acyl Migration and Its Implications in Lipid Modifications. By Anna MlLLQVIST FUREBY, PATRICK ADLERCREUTZ, and BO MATTIASSON .......... 231 Determination of Rate Constants in Lipase-catalyzed Esterification of Two Fatty Acids with Dodecanol. By Thomas Gitlesen, Patrick Adlercreutz, and Bo Mattiasson ............................................................ 238 The Effects of Cosolvent and Incubation Temperature on the Enantioselectivity of Aliphatic Ketone Reductions Catalyzed by Thermostable Secondary Alcohol Dehydrogenases. By Daniel L. Graham, Helen D. Simpson, and Donald A. Cowan .............................. 244 Control of Ionization State and Activity of Enzymes in Organic Media. By Peter J. Halling, Anne D. Blackwood, and Barry D. Moore ............ 251 Substrate Specificity and Kinetics of Candida rugosa Lipase in Organic Media. By Anja E. M. Janssen, Atul M. Vaidya, and Peter J. Halling ........................................................................................................... 257 Temperature Effects on Protease-catalyzed Peptide Synthesis in Organic Media. By Åsa Jönsson, Ernst Wehtje, Patrick Adlercreutz, and BoMattiasson .............................................................................................. 262 Interesterification Biocatalysis of Purified Lipase Fractions from Rhizopus niveus. By S. Kermasha and M. Safari ........................................................ 268 EPR Studies of Cutinase in Microemulsions. By V. Papadimitriou, A. Xenakis, C. T. Cazianis, H. Stamatis, M. Egmond, and F. N. Kolisis... 275 Kinetics of Peptide Condensation in Aqueous-Organic Solvent: Progressive Inactivation and Catalytic Reaction. By Shigeru Kunugi and Masumi Yoshida ........................................................................................................... 281 Xylanase Produced by Bacillus thermoantarcticus, a New Thermophilic Bacillus. By Licia Lama, Barbara Nicolaus, Valeria Calandrella Enrico Esposito, and Agata Gambacorta.............................................. 284 Influence of Environment Modifications on Enzyme Catalysis: Comparison of Macromolecular and Molecular Effects of Cosolvents on Lipoxygenase Reactions. By Hugues Berry, Christine Lambert, and Véronique Larreta-Garde ............................................................................................. 290 Heat-proof Enzymes by Thin Film Technology. By G NicOLiNi ........................ 297 An Organic Solvent-tolerant Bacterium and Its Organic Solvent-stable Protease. By Hiroyasu Ogino, Kiyoshi Yasui, Fumitake Watanabe, and Haruo Ishikawa ..................................................................................... 311 Medium and Biocatalyst Engineering as a Means to Affect Enzyme Activity and Stability in Organic Media. By Angeliki Öste-Triantafyllou, Ernst Wehtje, Patrick Adlercreutz, and Bo Mattiasson ................. 318 Conformational Changes of Different Isolipases from Candida rugosa in Liquid Interfaces and after Their Contact with Low-Water-Content Media. By G Otero, I. Del- Val, L. Robledo, C. Torres, J. A. Arcos, and J. Pérez-Gil ............................................................................................. 324 The Effect of Water Activity on Modified Lipase during Esterification. By A. B. Salleh, M. Таїв, M. Basri, К. Ampón, W. M. Z. YuNUS, and С. N. A. Razak ................................................................................................ 328 Alkaline and Thermophilic Amylases of Industrial Use. By Ryu Shinke, Kenji Aoki, Shuichiro Murakami, Takáto Inoue, and Tomoya Baba ...... 332 Characterization of Thermostability of Clostrìdium stercorarium Xylanase. By Kazuo Sakka, Masayuki Fukumura, Akiyoshi Tanaka, and Kunio Ohmiya ............................................................................................................ 341 Ammonolysis of Carboxylic Esters Catalyzed by Lipases: Synthesis of Fatty Acid Amides. By M. C De Zoete, A. C. Kock-Van Dalen, F. Van Rantwijk, and R. A. Sheldon ..................................................................... 346 Control of Water Activity in Organic Medium. By Ernst Wehtje, Jasmedh Kaur, Ingemar Svensson, Patrick Adlercreutz, and Bo Mattiasson ..................................................................................................... 351 Enhancing the Stereoselectivity and Activity of Candida Species Lipase in Organic Solvent by Noncovalent Enzyme Modification. By Hong Yang, Shu-Gui Cao, Si-Ping Han, Ni-Ni Guo, Xiu-Gong Gao, Zhong-Li Huang, Huan Dong, Nian-Xiang Zhang, Tong-Su Yang, Ying Chu, and Jia-Li Xu .................................................................................................. 358 Stopped-Flow, Pre-Steady-State Kinetic Study of Horseradish Peroxidase Catalysis in Nonaqueous Media. By Shunguang Wang, Wei Liu, Xinsong Ji, Lin Ma, Tiejin Li, and Zhongyi Yuan ................................... 364 Part IV. Application of Protein Expression in Biocatalysis Fusion Proteins: Expression and Function. By Leif Bülow and Klaus Mosbach ......................................................................................................... 376 Molecular Engineering of Streptavidin. By Takeshi Sano, Sándor Vajda, Gabriel O. Rezník, Cassandra L. Smith, and Charles R. Cantor ..... 383 Engineering Pathways in Escherichia coli for the Synthesis of Morphine Alkaloid Analgesics and Antitussives. By Anne M. Hailes, Christopher E. French, Deborah A. Rathbone, and Neil C. Bruce .......................... 391 Engineering Factor X Fusions for Expression in Pichia pastoris. By M. M. Guarna, H. С F. Còte, E. A. Amandoron, R. T. A. MacGillivray, R. A. J. Warren, and D. G. Kilburn ........................................................... 397 Cloning and Overexpression of Thermostable D-Hydantoinase from Thermophile in E. coli and Its Application to the Synthesis of Optically Active D-Amino Acids. By Dong-Cheol Lee, Seung-Goo Lee, Seung- Pyo Hong, Moon-Hee Sung, and Hak-Sung Kim ..................................... 401 Recent Advances in Cell-free Protein Synthesis towards a Protein Biosynthesizer. By Hideo Nakano, Yasuaki Kawarasaki, and Tsuneo Yamane ........................................................................................................... 406 Distance Mapping as a Tool in Protein Modification. By V. Nagarajan, Vasantha Pattabhi, and P. V. Sundaram ................................................. 413 Cellulose-binding Domains: Versatile Affinity Tags for Inexpensive Large- Scale Purification, Concentration, and Immobilization of Fusion Proteins. By P. Tomme, N. R. Gilkes, M. M. Guarna, C. A. Haynes, D. Hasenwinkle, E. Jervis, P. Johnson, L. McIntosh, R. A. J. Warren, and D. G. Kilburn .......................................................................................... 418 Purification and Properties of Cyclodextrinase from Bacillus stearothermophilus HY-1. By Ѕнои Jun Yang, Zhong Wang, and Shu Zheng Zhang ................................................................................................. 425 Purification of L-Aspartase by Gene Fusion. By HoNGYiNG Zhang, Xiaoping Wang, Jin Zhang, Jun Lu, and Wenyuan Du ......................... 429 Part V. Biochemical Engineering of Enzyme Systems Carbon-Carbon Bond Synthesis: Reactor Design and Operation for Transketolase-catalyzed Biotransformations. By John M. Woodley, Robin K. Mitra, and Malcolm D. Lilly .................................................... 434 Solubilization of Enzymes in Apoiar Solvents via Noncovalent Complex Formation with Soluble Polymers. By Bo Mattiasson, Marina Otamiri, and Patrick Adlercreutz .......................................................... 446 Purification of Urokinase by Affinity Cross-flow Filtration Using a Water- soluble Macroligand. By Xue Jun Cao, Jia Wen Zhu, Da Wei Wang, Xing Yan Wu, and Gan Ce Dai ................................................................... 454 Odor Removal from Amerhold DR-25 by Hydrolysis of Ethyl Propionate Using Enzyme Catalysts. By Kevin A. DiGregorio .................................... 460 Stability of Immobilized Penicillin Acylase under Reactive Conditions. By Andres Illanes, Claudia Altamirano, and Andrea Ruiz ................... 472 Application of Microbial Enzymes in Diagnostic Analysis. By Gaoxiang Li, Jianguo Liu, Ming Yin, Jian Guo, and Shunzhen Xie ............................ 476 The Effect on the Response to L-Lactate and H2O2 of a Carbon Paste Electrode Modified with Lactate Oxidase and Peroxidases Also Containing Different Additives and Solvents. By D. Narasaiah, U. Spohn, and L. Gorton .............................................................................. 482 Optimization of Glucose-l-Phosphate Production Employing Glucan- Phosphorylases in Continuous Enzyme Membrane Reactors. By Richard Griessler, Andreas Weinhäusel, Dietmar Haltrich, Klaus D. Kulbe, and Bernd Nidetzky....................................................................... 494 Real-Time Biomolecular Interaction Studies Based on Peroxidase Label and Chemiluminescent Flow-Injection System. By Alexander P. Osipov, Nataliya V. Zaitseva, and Alexey M. Egorov ...................................... 501 Amperometric Enzyme-channeling Immunosensor. By J. RiSHPON and D. Ivnitski ....................................................................................................... 508 Performance of the Apoenzyme-Membrane Sensors for Microdetermination of Heavy Metal Ions. By Ικυο Satoh ............................................................ 514 Amplifying Bienzyme Cycle-linked Immunoassays for Determination of 2,4-Dichlorophenoxyacetic Acid. By Frank F. Bier, Eva Ehrentreich-Förster, and Frieder W. Scheller ................................. 519 Lacease: A Marker Enzyme for Solvent-modified Immunoassays. By W. F. M. Stöcklein and F. W. Scheller .................................................................... 525 Activity of Nisin Immobilized in a Hollow-Fiber Reactor. By ANNA Lante, Gabriella Pasini, Antonella Crapisi, Paolo Scalabrini, and Paolo Spettoli .............................................................................................. 529 Immobilization of Aminoacylase on an Anion Exchange Column to Be Used as a Chromatographie Reactor. By M. L. JANSEN, E. VAN ZESSEN, A. J. J. Straathof, L. A. M. van der Wielen, K. Ch. A. M. Luyben, and W. J. J. VAN DEN TWEEL.................................................................................. 533 Biomolecular Modules for Creatinine Determination. By Axel Warsinke, Alexander Benkert, Werner Schössler, and Frieder Scheller..... 541 In Situ Product Removal from E. coli Transketolase-catalyzed Biotransformations. By Rashmi P. Chauhan, John M. Woodley, and Lawson W. Powell ....................................................................................... 545 Use of Enzyme Technology to Convert Waste Lactose into Valuable Products. By David E. Stevenson, Roger A. Stanley, and Richard H. Furneaux .................................................................................................. 555 Development of a Pilot-Plant Fermentation Process for the Production of Yeast Lactase. By F. Acevedo, J. C. Gentina, N. Bojorge, I. Reyes, and A. Torres ................................................................................................. 559 Kinetic Studies oiFusarium solani Cutinase in a Gas/Solid System. By S. Lamare and M. D. Legoy ......................................................................... 563 A Stable Cholesterol Esterase. By R. Venkatesh and P. V. Sundaram .......... 569 Rigid Proteases Are More Stable. By R. Venkatesh, N. Rajalakshmi, J. Sivakumar, Peter Sellers, and P. V. Sundaram ................................. 571 Reaction Engineering Aspects of Activated Sugar Production: CMP-NeuőAc as an Example. By Udo Kragl, Teresa Klein, Djurda Vasic-Racki, Matthias Kittelmann, Oreste Ghisalba, and Christian Wandrey .. 577 Part VI. Biomaterials Synthesis and Design Enzymatically Modified Alginates as Useful Biopolymers. By Donal F. Day ....... 584 Lipase-catalyzed Synthesis of Lactone and Biodegradable Polyester in Organic Solvent. By Huan Dong, Shu-Gui Cao, Hong-Da Wang, Si-Ping Han, Nian-Xiang Zhang, and Hong Yang ................................ 588 Enzymatic Regioselective Transesterification of Sugar Alcohols and Aromatic Esters in Organic Solvents. By Hyun Gyu Park, Ho Nam Chang, and Jonathan S. Dordick .............................................................. 595 Production of Levan, a Fructose Polymer, Using an Overexpressed Recombinant Levansucrase. By Ki-Bang Song, Hafedh Belghith, and Sang-Ki Rhee .......................................................................................... 601 The Optimization and Semicontinuous Synthesis of Oligosaccharides. By N. K. Smith, S. G. Gilmour, and R. A. Rastall ........................................ 608 Part VII. Enzymes in Organic Synthesis The Application of Asymmetric Bioreductions to the Production of Chiral Pharmaceutical Drugs. By Michel Chartrain, Joseph Armstrong, Lorraine Katz, Steven King, Jayanthi Reddy, Yao-Jun Shi, David Tschaen, and Randolph Greasham .......................................................... 612 Practical Enzymatic Resolution of Racemic Alcohols and Amines in Organic Solvents. By Arie L. Gutman, Eleonora Shkolnik, Elazar Meyer, Felix Polyak, Dov Brenner, and Aviv Boltanski................................. 620 Enzymatic Production of a-Butylglucoside and Its Fatty Acid Esters. By Pierre F. Monsan, François Paul, Vincent Pelenc, and Emmanuel Boures ............................................................................................................ 633 Synthetic Applications of NAD(P)(H)-dependent Enzymes. By GIACOMO Carrea, Gì anluca Ottolina, Piero Pasta, and Sergio Riva .............. 642 Optical Resolution of Pantolactone by a Novel Fungal Enzyme, Lactonohydrolase. By Sakayu Shimizu and Michihiko Kataoka............ 650 Enantioselective Penicillin Acylase-catalyzed Reactions: Factors Governing Substrate and Stereospecificity of the Enzyme. By V. K. ŠVEDAS, M. V. Savchenko, A. I. Beltser, and D. F. Guranda ........................................ 659 Enzymatic Preparation of Monoglycerides via Glycerolysis of Fats and Oils Catalyzed by Lipase from Pseudomonas Species. By Shu-Gui Cao, Xiu-Gong Gao, and Ke-Chang Zhang ...................................................... 670 Enzymatic Transesterification of a Triacylglycerol Mixture: Application to Butterfat Modification. By L. Lamboursain, A. Marty, S. Kermasha, J. S. Condoret, and D. Combes ................................................................... 678 Microbial D-Amino Acid Oxidases (EC 1.4.3.3). By Lutz Fischer, Matthias Gabler, Roy Hörner, and Fritz Wagner ............................. 683 Enzymatic Resolution of {R,S)-2-Octano Catalyzed by Lipase from Pseudomonas Species. By Xiu-Gong Gao, Shu-Gui Cao, Hong Yang, Ni-Ni Guo, and Ke-Chang Zhang ............................................................... 689 Novel Sol-Gel Matrices for the Immobilization of Enzymes. By Iqbal Gill and Antonio Ballesteros ........................................................................... 697 Production and Stabilization of Pure Maltose Phosphorylase from Lactobacillus brevis for Sensing Inorganic Phosphate. By S. ffiJWEL, L. Haalck, N. Conrath, and F. Spener ...................................................... 701 (5)-Hydroxynitrile Lyase from Hevea brasiliensis. By Meinhard Hasslacher, Michael Schall, Marianne Hayn, Herfried Griengl, Sepp D. Kohlwein, and Helmut Schwab ................................................... 707 Chiral Alcohols by Enantioselective Enzymatic Oxidation. By W. HUMMEL andB. Riebel ...........................................................................■...................... 713 Optical Resolution of Racemic 1-Phenylethylamine Catalyzed by Aminotransferase and Dehydrogenase. By Jong-Shik Shin and Byung- GeeKim ........................................................................................................... 717 New Microbial Amidases. By M-R. Kula, U. Joeres, and U. Stelkes- Ritter .............................................................................................................. 725 Carbon-Carbon Bond Synthesis: Preparation and Use of Immobilized Transketolase. By Simon P. Brocklebank, Robin K. Mitra, John M. Woodley, and Malcolm D. Lilly .............................................................. 729 Lipase-catalyzed Ester Synthesis. By Yu-Yen Linko, Zhuo-Lin Wang, Merja Lämsä, and Jukka Seppälä .............................................................. 737 Acceptor Reactions of Levansucrase from Bacillus circulons. By M. A. PÉREZ OSEGUERA, G. L. GUERECA, and A. LÓPEZ-MUNGUÍA ................................ 743 Lipase-catalyzed Selective Esterification of Ibuprofen. By A. Ducret, P. Pepin, M. Trani, and R. Lortie ............................................................... 747 Inactivation of Glucose-Fructose Oxidoreductase from Zymomonas mobilis during Its Catalytic Action. By Monika Fürlinger, Bernd Nidetzky, Robert К. Scopes, Dietmar Haltrich, and Klaus D. Kulbe................ 752 Application of Vinyl Esters for the Lipase-catalyzed High-Yield Synthesis of Monoacylglycerols. By U. Bornscheuer, L. Gaziola, and R. D. Schmid ....... 757 Enzymatic Preparation of Optically Active Silicon-containing Amino Acids. By Atsuo Tanaka, Hayato Yamanaka, and Takuo Kawamoto ........... 762 Synthesis of Adenosine S -Triphosphate Derivatives and Their Substrate Activities to Some Glucokinases. By KOSUKÉ Tomita, Kanehisa Kojoh, and Atsushi Suzuki ....................................................................................... 766 Enantioselective Synthesis of Aliphatic (5)-Cyanohydrins in Organic Solvents Using Hydroxynitrile Lyase from Manihot esculenta. By Harald Wajant, Siegfried Förster, Achim Sprauer, Franz Effenberger, and Klaus Pfizenmaier....................................................................................... 771 Cross-Linked Crystals of Subtilisin: Versatile Catalysts for Organic Synthesis. By Yi-Fong Wang, Kirill Yakovlevsky, Nazer Khalaf, Bailing Zhang, and Alexey L. Margolin ............................................................... 777 Enzymatic Preparation of d(—)-Tartaric Acid from ds-Epoxysuccinic Acid by Pseudomonas putida MCI3037. By Kenji Yamagishi and Hiroshi Сно .... 784 Index of Contributors ............................................................................................ 787 The New York Academy of Sciences believes it has a responsibility to provide an open forum for discussion of scientific questions. The positions taken by the participants in the reported conferences are their own and not necessarily those of the Academy. The Academy has no intent to influence legislation by providing such forums.
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language	English
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series	New York Academy of Sciences: Annals of the New York Academy of Sciences
series2	New York Academy of Sciences: Annals of the New York Academy of Sciences Annals of the New York Academy of Sciences
spelling	Enzyme engineering 13 ed. by A. I. Laskin ... 1996 New York, NY XV, 790 S. Ill., zahlr. graph. Darst. txt rdacontent n rdamedia nc rdacarrier New York Academy of Sciences: Annals of the New York Academy of Sciences 799 Annals of the New York Academy of Sciences ... Encimi - Biotehnologija - Posvetovanja ssg (DE-588)1071861417 Konferenzschrift gnd-content Laskin, Allen I. Sonstige oth Dordick, Jonathan S. Sonstige oth International Enzyme Engineering Conference Sonstige (DE-588)131227-3 oth (DE-604)BV000253031 13 New York Academy of Sciences: Annals of the New York Academy of Sciences 799 (DE-604)BV002532608 799 Digitalisierung TU Muenchen application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007485870&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Enzyme engineering New York Academy of Sciences: Annals of the New York Academy of Sciences Encimi - Biotehnologija - Posvetovanja ssg
subject_GND	(DE-588)1071861417
title	Enzyme engineering
title_auth	Enzyme engineering
title_exact_search	Enzyme engineering
title_full	Enzyme engineering 13 ed. by A. I. Laskin ...
title_fullStr	Enzyme engineering 13 ed. by A. I. Laskin ...
title_full_unstemmed	Enzyme engineering 13 ed. by A. I. Laskin ...
title_short	Enzyme engineering
title_sort	enzyme engineering
topic	Encimi - Biotehnologija - Posvetovanja ssg
topic_facet	Encimi - Biotehnologija - Posvetovanja Konferenzschrift
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007485870&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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work_keys_str_mv	AT laskinalleni enzymeengineering13 AT dordickjonathans enzymeengineering13 AT internationalenzymeengineeringconference enzymeengineering13

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