Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
1996
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | [4], 81 Bl. Ill., graph. Darst. |
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| 100 | 1 | |a Altwasser, Michael |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis |c von Michael Altwasser |
| 264 | 1 | |c 1996 | |
| 300 | |a [4], 81 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
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Datensatz im Suchindex
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CONTENTS
PAGE
1. SUMMARY.1
1.1. SUMMARY.1
1.2. ZUSAMMENFASSUNG.2
2. INTRODUCTION.4
2.1. REGULATION OF GENE EXPRESSION IN EUCARYOTES. 5
2.1.1. TRANSCRIPTIONAL REGULATION
.5
2.2. RNA-POLYMERASE ACTIVITY IN
XENOPUS OOCYTES AND EMBRYOS 6
2.3. RNA POLYMERASE I TRANSCRIPTION 8
2.4. SL1, TIF-IB, FACTOR D AND RIB1.9
2.5. UBF: A POLYMERASE L-SPECIFIC TRANSCRIPTION FACTOR 10
2.5.1. VARIANTS OF THE XENOPUS LAEVIS
UBF 12
2.5.2. STRUCTURE OF THE XENOPUS LAEVIS
UBF.13
2.5.3. UBF AND POLYMERASE I ACTIVITY.14
2.6. OUTLINE OF THIS DISSERTATION 1 5
3. RESULTS 16
3.1. UBF ON THE RNA LEVEL.16
3.1.1. UBF MRNA IN DIFFERENT DEVELOPMENTAL STAGES OF XENOPUS
16
3.2. UBF ON THE PROTEIN LEVEL.17
3.2.1. UBF PROTEINS IN DIFFERENT FROG TISSUES.17
3.2.2. UBF PROTEINS IN DIFFERENT DEVELOPMENTAL STAGES OF XENOPUS
.19
3.2.3. WHEN IS UBF SYNTHESIZED?.21
3.2.3.1. IMMUNOPRECIPITATION OF UBF.
2 2
3.2.3.2. UBF PROTEIN SYNTHESIS.2
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/948810009
PAGE
3.3. PHOSPHORYLATION OF UBF.26
3.3.1. IN VITRO PHOSPHORYLATION OF IN VITRO SYNTHESIZED UBF.2 6
3.3.2. UBF PHOSPHORYLATION IN OOCYTES.2 7
3.3.3. UBF PHOSPHORYLATION IN EMBRYOS.2 7
3.3.2.
IN VITRO PHOSPHORYLATION OF ENDOGENOUS UBF.2 8
3.3.5. TOOLS FOR
IN VIVO STUDIES.2 8
3.3.5.1. CLONING OF PLASMIDS FOR
IN VIVO STUDIES.2 9
3.3.5.2. OVEREXPRESSION OF UBF.3 0
3.3.5.3. STABILITY OF
IN VITRO SYNTHESIZED UBF RNA IN EMBRYOS 31
3.3.5.4. INJECTION EXPERIMENTS WITH
IN VITRO SYNTHESIZED PROTEIN.3 2
3.3.6.
IN VIVO PHOSPHORYLATION OF IN VITRO SYNTHESIZED UBF.3 2
3.3.6.1. PHOSPHORYLATION OF
IN VITRO SYNTHESIZED UBF IN OOCYTES 3 2
3.3.6.2.
PHOSPHORYLATION OF IN VITRO SYNTHESIZED UBF IN EMBRYOS.3 3
3.7. UBF LOCALIZATION WITHIN THE CELL.3 3
3.7.1. LOCALIZATION OF UBF WITHIN OOCYTES.3 3
3.7.2. LOCALISATION OF UBF WITHIN EMBRYOS 3 5
3.8. UBF, AN ACTIVATING FACTOR OF POLYMERASE I ? 3 8
4. DISCUSSION.40
4.1. UBF ON THE RNA LEVEL.4 0
4.2. UBF ON THE PROTEIN LEVEL.41
4.3. PHOSPHORYLATION OF UBF.4 2
4.4. LOCALIZATION OF UBF.43
4.5. DIFFERENT RNA POLYMERASE I INACTIVATION AND ACTIVATION SYSTEMS.44
5. MATERIALS AND METHODS
PAGE
50
5.1. MATERIALS
5Q
5.1.1. CHEMICALS AND OTHER SUPPLIES
5 0
5.1.
2. ENZYMES AND KITS 5 0
5.1.3. BUFFERS AND MEDIA
5 -J
5.1.4. PLASMIDS
53
5.1.5. ANTIBODIES
53
5.1.6. CO//' STRAINS
5 4
5.1.7. FROGS.
54
5.1.8. INSTRUMENTS
55
5.1.9. COMPUTERS AND PROGRAMS
55
5.2. METHODS
5 5
5.2.1. METHODS IN MOLECULAR BIOLOGY
5 5
5.2.1.1. DNA TECHNIQUES
55
5.2.1.2. RNA TECHNIQUES
57
5.2.1.3. PROTEIN TECHNIQUES 5 8
5.2.1.4. PCR AND REVERSE TRANSCRIPTASE REACTIONS
5 9
5.2.2. FROG SPECIFIC TECHNIQUES
6 0
5.2.2.1. FROGS.
6 0
5.2.2.2. OOCYTES.
6 0
5.2.2.3. EMBRYOS.
6 1
5.2.3. MICROINJECTION
6 2
5.2.4. IMMUNOFIUORESCENT STAINING
6 2
6
. LITERATURE
PAGE
63
7. APPENDIX.
7.1. ABBREVIATIONS
7.2. ACKNOWLEDGMENT
7.3. CURRICULUM VITAE
7.3.1. CURRICULUM VITAE
78
7
8
7 9
8
0
.80
81
7.3.2. LEBENSLAUF |
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| indexdate | 2024-10-09T16:11:34Z |
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| language | German |
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| physical | [4], 81 Bl. Ill., graph. Darst. |
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| spelling | Altwasser, Michael Verfasser aut Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis von Michael Altwasser 1996 [4], 81 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Berlin, Freie Univ., Diss., 1996 Glatter Krallenfrosch (DE-588)4157477-1 gnd rswk-swf Transkriptionsfaktor (DE-588)4303350-7 gnd rswk-swf Oogenese (DE-588)4043568-4 gnd rswk-swf RNS-Polymerase I (DE-588)4353281-0 gnd rswk-swf Embryonalentwicklung (DE-588)4070792-1 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Glatter Krallenfrosch (DE-588)4157477-1 s Oogenese (DE-588)4043568-4 s Transkriptionsfaktor (DE-588)4303350-7 s RNS-Polymerase I (DE-588)4353281-0 s Genexpression (DE-588)4020136-3 s DE-604 Embryonalentwicklung (DE-588)4070792-1 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007430965&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Altwasser, Michael Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis Glatter Krallenfrosch (DE-588)4157477-1 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Oogenese (DE-588)4043568-4 gnd RNS-Polymerase I (DE-588)4353281-0 gnd Embryonalentwicklung (DE-588)4070792-1 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4157477-1 (DE-588)4303350-7 (DE-588)4043568-4 (DE-588)4353281-0 (DE-588)4070792-1 (DE-588)4020136-3 (DE-588)4113937-9 |
| title | Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis |
| title_auth | Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis |
| title_exact_search | Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis |
| title_full | Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis von Michael Altwasser |
| title_fullStr | Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis von Michael Altwasser |
| title_full_unstemmed | Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis von Michael Altwasser |
| title_short | Regulation of gene expression of the RNA polymerase I transcription factor UBF during oogenesis and early embryogenesis of Xenopus laevis |
| title_sort | regulation of gene expression of the rna polymerase i transcription factor ubf during oogenesis and early embryogenesis of xenopus laevis |
| topic | Glatter Krallenfrosch (DE-588)4157477-1 gnd Transkriptionsfaktor (DE-588)4303350-7 gnd Oogenese (DE-588)4043568-4 gnd RNS-Polymerase I (DE-588)4353281-0 gnd Embryonalentwicklung (DE-588)4070792-1 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | Glatter Krallenfrosch Transkriptionsfaktor Oogenese RNS-Polymerase I Embryonalentwicklung Genexpression Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007430965&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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