Analytical parasitology:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | German |
| Veröffentlicht: |
Berlin [u.a.]
Springer
1997
|
| Schriftenreihe: | Springer lab manual
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 364 S. graph. Darst. |
| ISBN: | 3540589198 |
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Datensatz im Suchindex
| _version_ | 1804125581153927168 |
|---|---|
| adam_text | MICHAEL T. ROGAN (ED.) ANALYTICAL PARASITOLOGY WITH 24 FIGURES SPRINGER
CONTENTS CHAPTER 1 BIOCHEMICAL PATHWAYS IN PARASITES JOHN BARRETT . . .
1 1.1 INTRODUCTION 1 1.2 PREPARATION OF ENZYME FRACTIONS 1 1.3 ENZYME
ASSAYS 6 1.4 METABOLITE MEASUREMENTS 15 1.5 USE OF INHIBITORS 27
REFERENCES 29 CHAPTER 2 ELECTROPHORESIS OF PARASITE PROTEINS EILEEN
DEVANEY 32 2.1 INTRODUCTION 32 2.2 LYSIS OF PARASITES AND EXTRACTION OF
PROTEINS 32 2.2.1 SOLUBILISATION IN SDS SAMPLE COCKTAIL 33 2.2.2
DETERGENT SOLUBILISATION 33 2.3 SDS-PAGE 36 2.4 MODIFICATIONS OF
STANDARD METHOD 47 2.4.1 GRADIENT GELS 47 2.4.2 TRICINE GELS FOR THE
ANALYSIS OF VERY LOW MOLECULAR WEIGHT POLYPEPTIDES . . 48 2.5
APPLICATIONS OF SDS-PAGE TO THE CHARACTERISATION OF INDIVIDUAL
POLYPEPTIDES 50 2.5.1 GLYCOPROTEIN ANALYSIS USING SPECIFIC ENZYMES 51
2.5.2 PEPTIDE MAPPING 53 2.5.3 PREPARATIVE SDS-PAGE ARID ELECTROCUTION
... 55 2.6 2D GEL ELECTROPHORESIS 57 2.6.1 NON-EQUILIBRIUM PH GRADIENT
ELECTROPHORESIS (NEPHGE) 59 X CONTENTS 2.6.2 ISOELECTRIC FOCUSING (IEF)
63 REFERENCES 65 CHAPTER 3 MOLECULAR TECHNIQUES IN ANALYTICAL
PARASITOLOGY STEVEN HEATH 66 3.1 INTRODUCTION 66 3.2 ISOLATION OF
PARASITE NUCLEIC ACID 66 3.2.1 PREPARATION OF DNA 67 3.2.2 PREPARATION
OF RNA 68 3.2.3 QUANTIFICATION OF NUCLEIC ACID 69 3.3 GEL
ELECTROPHORESIS AND BLOTTING OF NUCLEIC ACID 69 3.3.1 GEL
ELECTROPHORESIS 69 3.3.2 BLOTTING OF NUCLEIC ACID 72 3.4 LABELLING AND
HYBRIDISATION OF DNA PROBES 73 3.4.1 RADIOLABELLING OF DNA PROBES 73
3.4.2 NON-ISOTOPIC LABELLING OF DNA PROBES 75 3.4.3 HYBRIDISATION AND
SIGNAL DETECTION 76 3.5 POLYMERASE CHAIN REACTION (PCR) 78 3.5.1 REVERSE
TRANSCRIPTASE - PCR (RT-PCR) 79 3.5.2 TOUCHDOWN PCR 81 REFERENCES 82
CHAPTER 4 THE PRODUCTION AND ANALYSIS OF HELMINTH EXCRETORY-SECRETORY
(ES) PRODUCTS ALAN BROWN, GARY GRIFFITHS, PETER MICHAEL BROPHY, BARBARA
ANNE FURMIDGE, AND DAVID IDRIS PRITCHARD 83 4.1 INTRODUCTION 83 4.2
COLLECTION OF EXCRETORY-SECRETORY (ES) PRODUCTS 84 4.3
ACETYLCHOLINESTERASE 91 4.3.1 QUANTITATION OF ACETYLCHOLINESTERASE
ACTIVITY.. 92 4.3.2 DETECTION OF ACHE ACTIVITY FOLLOWING POLYACRYLAMIDE
GEL ELECTROPHORESIS . 95 4.3.3 PURIFICATION OF ACHE 95 4.3.4
IDENTIFICATION AND SEPARATION OF ACHE MOLECULAR FORMS 98 4.3.5 KINETIC
PROPERTIES OF ACHE 99 CONTENTS XI 4.4 PROTEOLYTIC ENZYMES 99 4.5
ANTICOAGULANTS, BLOOD COAGULATION ASSAYS ... 100 4.5.1 THE COLLECTION OF
PLASMA 101 4.5.2 RECALCIFICATION TIME TEST (INTRINSIC PATHWAY) . 103
4.5.3 ACTIVATED PARTIAL THROMBOPLASTIN TEST (INTRINSIC PATHWAY) 104
4.5.4 PROTHROMBIN TIME TEST (EXTRINSIC PATHWAY) . . 105 4.5.5 STYPVEN
CLOTTING TIME TEST (COMMON PATHWAY) 105 4.5.6 PLATELET AGGREGATION 106
4.6 POTENTIAL IMMUNOMODULATORS . 110 4.6.1 GLUTATHIONE S-TRANSFERASES
110 4.6.2 SUPEROXIDE DISMUTASE (SODS) 115 4.6.3 DETECTION OF
IMMUNOMODULATIPN OF ANTIBODY PRODUCTION 117 4.6.4 NEUTROPHIL INHIBITORY
FACTOR (NIF) 120 4.7 CONCLUDING REMARKS 124 REFERENCES 125 CHAPTER 5
PARASITE PROTEINASES MICHAEL J. NORTH 133 5.1 INTRODUCTION 133 5.2
SAMPLE PREPARATION 138 5.3 SUBSTRATES AND PROTEINASE ASSAYS 139 5.3.1
OPTIMAL CONDITIONS FOR PROTEINASE ACTIVITY .. 139 5.3.2 PROTEIN AND
PEPTIDE SUBSTRATES 140 5.3.3 SYNTHETIC FLUOROGENIC AND CHROMOGENIC
SUBSTRATES 148 5.3.4 EXOPEPTIDASE SUBSTRATES 154 5.4 PROTEINASE
INHIBITORS 158 5.4.1 STANDARD PROCEDURES FOR ENZYME INHIBITION .. 158
5.4.2 THE SELECTION AND DESIGN OF SPECIFIC INHIBITORS 160 5.5
ELECTROPHORETIC METHODS 161 5.5.1 SUBSTRATE-SDS-PAGE - GENERAL PROCEDURE
... 161 5.5.2 SUBSTRATE-SDS-PAGE - ADDITIONAL TECHNIQUES 167 5.5.3
AFFINITY LABELLING 168 5.6 PURIFICATION METHODS 170 5.7 ENDOGENOUS
INHIBITORS 177 5.8 FINAL COMMENTS 178 REFERENCES 180 XII CONTENTS
CHAPTER 6 NEUROBIOLOGY OF HELMINTH PARASITES AARON G. MAULE, DAVID W.
HALTON, TIMOTHY A. DAY, RALPH A. PAX, AND CHRIS SHAW 187 6.1
INTRODUCTION 187 6.2 IMMUNOCYTOCHEMICAL LOCALISATION OF NEUROACTIVE
SUBSTANCES IN HELMINTH PARASITES 187 6.2.1 PRODUCTION OF ANTISERA 188
6.2.2 IMMUNOCYTOCHEMISTRY PROTOCOL 191 6.2.3 CONFOCAL SCANNING LASER
MICROSCOPY (CSLM) . 197. 6.2.4 ELECTRON MICROSCOPE IMMUNOGOLD LABELLING
. . 198 6.3 CHARACTERISATION AND PURIFICATION OF NEUROPEPTIDES 200 6.3.1
TISSUE EXTRACTION 201 6.3.2 PEPTIDE DETECTION 205 6.3.3 PRIMARY
PURIFICATION STEPS 207 6.3.4 REVERSE-PHASE HPLC 208 6.3.5 ENDOPROTEINASE
DIGESTION 213 6.4 PHYSIOLOGICAL ACTION OF TRANSMITTERS AND DRUG
ASSESSMENT IN VITRO 215 6.4.1 RECORDING OF MOTOR ACTIVITY IN WHOLE
ANIMALS OR IN MUSCLE STRIPS 215 6.4.2 ACTION OF TRANSMITTERS AND DRUGS
AT THE CELLULAR LEVEL 219 REFERENCES 224 CHAPTER 7 ELECTRON MICROSCOPY
IN PARASITOLOGY ANDREW HEMPHILL AND SIMON L. CROFT .... 227 7.1
INTRODUCTION 227 7.2 PREPARATION FOR TRANSMISSION ELECTRON MICROSCOPY
(TEM) 228 7.2.1 FIXATION: GENERAL BACKGROUND 229 7.2.2 STANDARD FIXATION
METHODS 233 7.2.3 STANDARD FIXATION PROTOCOLS 234 7.3 CYTOCHEMISTRY AND
STAINING PROTOCOL IN TEM . 239 7.3.1 INTRODUCTION 239 7.3.2 STANDARD
STAINS 240 7.3.3 SPECIAL CYTOCHEMICAL STAINS 241 7.3.4 ENZYME STAINS 242
CONTENTS XIII 7.4 PREPARATION FOR SCANNING ELECTRON MICROSCOPY (SEM) 243
7.5 IMMUNOGOLD-LABELLING TECHNIQUES 245 7.5.1 BASIC INFORMATION 245
7.5.2 APPROACHES FOR IMMUNOGOLD-LABELLING 251 7.5.3 APPLICATIONS OF
IMMUNOGOLD EM TECHNIQUES . 262 REFERENCES 263 CHAPTER 8 STUDIES OF THE
SURFACE PROPERTIES, LIPOPHILIC PROTEINS AND METABOLISM OF PARASITES BY
THE USE OF FLUORESCENT AND CAGED COMPOUNDS J. MODHA, C.A. REDMAN, S.
LIMA, M.W. KENNEDY, AND J. KUSEL 269 8.1 INTRODUCTION 269 8.2
INCORPORATION OF FLUORESCENT COMPOUNDS INTO MEMBRANES 270 8.2.1 SURFACE
MEMBRANE INCORPORATION OF FLUORESCENT COMPOUNDS 270 8.2.2 EXAMINATION OF
LOCATION OF FLUORESCENT PROBE IN MEMBRANE 276 8.2.3 UPTAKE OF
FLUORESCENT FATTY ACIDS AND CHOLESTEROL 281 8.2.4 LABELLING PARASITE
SURFACES WITH A STABLE CELL-LABELLING REAGENT (SIGMA, PKH2 AND PKH26)
282 8.3 USING FLUORESCENT TECHNIQUES TO MEASURE MEMBRANE FLUIDITY 284
8.4 DISTRIBUTION OF ACTIN 287 8.5 ASSESSMENT OF PARASITE MEMBRANE DAMAGE
. . 288 8.6 LOCALISATION OF PROBES IN ORGANELLES 290 8.7 MEASUREMENT OF
PH AT THE SURFACE OF PARASITES AND INTRACELLULAR PH 291 8.8 THE USE OF
CAGED COMPOUNDS 292 8.9 USE OF FLUORESCENT PROBES IN THE ANALYSIS OF
PARASITE PROTEINS 295 REFERENCES 301 XIV CONTENTS CHAPTER 9 DETECTION
AND IDENTIFICATION OF PARASITE SURFACE CARBOHYDRATES BY LECTINS GEORGE
A. INGRAM . 304 9.1 INTRODUCTION 304 9.2 LECTIN-MEDIATED TRYPANOSOMATID
PARASITE AGGLUTINATION 305 9.3 FLUORESCEIN-ISOTHIOCYANATE
(FITC)-CONJUGATED LECTINS 307 9.4 PEROXIDASE-LABELLED LECTINS 310 9.5
BIOTINYLATED LECTINS 310 9.6 FIXATIVES AND ENZYMATIC TREATMENTS 311 9.7
HISTOLOGICAL SECTIONS 312 9.8 CONTROLS 313 REFERENCES 317 CHAPTER 10
IMMUNOLOGICAL ANALYSIS OF PARASITE MOLECULES MICHAEL T. ROGAN 320 10.1
INTRODUCTION 320 10.2 PARASITE EXTRACTS 321 10.3 PRODUCTION OF
ANTIBODIES TO PARASITE MOLECULES 77, 323 10.3.1 PRODUCTION OF POLYCLONAL
ANTIBODIES 324 10.3.2 PRODUCTION OF MONOCLONAL ANTIBODIES 328 10.3.3
LABELLING OF ANTIBODIES 336 10.4 ENZYME LINKED IMMUNOSORBENT ASSAYS
(ELISA) 338 10.4.1 THE BASIC TECHNIQUE 340 10.4.2 OPTIMIZING THE ASSAY
341 10.4.3 CAPTURE ELISA 345 10.5 IMMUNOBLOTTING 347 10.5.1 THE BASIC
TECHNIQUE 348 10.5.2 PROBING THE BLOT WITH ANTIBODY 349 10.6 AFFINITY
CHROMATOGRAPHY 353 10.6.1 ANTIBODY PURIFICATION 353 10.6.2 ANTIGEN
PURIFICATION 355 REFERENCES 358 SUBJECT INDEX 361
|
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| indexdate | 2024-07-09T18:03:50Z |
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| isbn | 3540589198 |
| language | German |
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| spelling | Analytical parasitology Michael T. Rogan (ed.) Berlin [u.a.] Springer 1997 XVII, 364 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer lab manual Parasitology cabt Diagnostic Techniques cabt Laboratory Diagnosis cabt Parasitologia (tecnicas) larpcal Parasitologia larpcal Diagnostic parasitology Laboratory manuals Parasitology methods Laboratory Manuals Analytische Methode (DE-588)4142352-5 gnd rswk-swf Parasitologie (DE-588)4122308-1 gnd rswk-swf Labormedizin (DE-588)4073931-4 gnd rswk-swf Parasitologie (DE-588)4122308-1 s Analytische Methode (DE-588)4142352-5 s DE-604 Labormedizin (DE-588)4073931-4 s Rogan, Michael T. Sonstige oth HEBIS Datenaustausch Mainz application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007430007&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Analytical parasitology Parasitology cabt Diagnostic Techniques cabt Laboratory Diagnosis cabt Parasitologia (tecnicas) larpcal Parasitologia larpcal Diagnostic parasitology Laboratory manuals Parasitology methods Laboratory Manuals Analytische Methode (DE-588)4142352-5 gnd Parasitologie (DE-588)4122308-1 gnd Labormedizin (DE-588)4073931-4 gnd |
| subject_GND | (DE-588)4142352-5 (DE-588)4122308-1 (DE-588)4073931-4 |
| title | Analytical parasitology |
| title_auth | Analytical parasitology |
| title_exact_search | Analytical parasitology |
| title_full | Analytical parasitology Michael T. Rogan (ed.) |
| title_fullStr | Analytical parasitology Michael T. Rogan (ed.) |
| title_full_unstemmed | Analytical parasitology Michael T. Rogan (ed.) |
| title_short | Analytical parasitology |
| title_sort | analytical parasitology |
| topic | Parasitology cabt Diagnostic Techniques cabt Laboratory Diagnosis cabt Parasitologia (tecnicas) larpcal Parasitologia larpcal Diagnostic parasitology Laboratory manuals Parasitology methods Laboratory Manuals Analytische Methode (DE-588)4142352-5 gnd Parasitologie (DE-588)4122308-1 gnd Labormedizin (DE-588)4073931-4 gnd |
| topic_facet | Parasitology Diagnostic Techniques Laboratory Diagnosis Parasitologia (tecnicas) Parasitologia Diagnostic parasitology Laboratory manuals Parasitology methods Laboratory Manuals Analytische Methode Parasitologie Labormedizin |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007430007&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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