Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
1996
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 77 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV010809302 | ||
| 003 | DE-604 | ||
| 005 | 19961105 | ||
| 007 | t | ||
| 008 | 960624s1996 ad|| m||| 00||| engod | ||
| 016 | 7 | |a 948216875 |2 DE-101 | |
| 035 | |a (OCoLC)258525186 | ||
| 035 | |a (DE-599)BVBBV010809302 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-91 |a DE-29T |a DE-12 |a DE-355 |a DE-83 |a DE-11 |a DE-188 | ||
| 100 | 1 | |a Glathe, Stefanie |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor |c vorgelegt von Stefanie Glathe |
| 264 | 1 | |c 1996 | |
| 300 | |a 77 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Berlin, Freie Univ., Diss., 1996 | ||
| 650 | 0 | 7 | |a Struktur-Aktivitäts-Beziehung |0 (DE-588)4183784-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Präkursor |0 (DE-588)4300438-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Epidermaler Wachstumsfaktor |0 (DE-588)4271160-5 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Epidermaler Wachstumsfaktor |0 (DE-588)4271160-5 |D s |
| 689 | 0 | 1 | |a Präkursor |0 (DE-588)4300438-6 |D s |
| 689 | 0 | 2 | |a Struktur-Aktivitäts-Beziehung |0 (DE-588)4183784-8 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007221552&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-007221552 | |
Datensatz im Suchindex
| _version_ | 1807323669098135552 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
ZUSAMMENFASSUNG
.
1
SUMMARY
.
3
INTRODUCTION
.
5
PROTEINS
CONTAINING
THE
EGF-MOTIF
AND
THEIR
DIVERSE
FUNCTIONS
.
7
GROWTH
FACTOR
PRECURSORS
.
8
THE
EPIDERMAL
GROWTH
FACTOR
(EGF)
PRECURSOR
.
10
THE
RECEPTOR
TYROSINE
KINASE
TYPE
I
FAMILY
.
11
OBJECTIVES
.
12
RESULTS.
.
14
BACTERIAL
FUSION
PROTEINS
AS
A
MODEL
SYSTEM
FOR
EXPRESSING
PUTATIVE
LIGANDS
.
14
EXPRESSION
OF
RECOMBINANT
PORTIONS
OF
HUMAN
PROEGF
.
16
PRO
5-9
IS
AN
ACTIVE
LIGAND
.
17
NONE
OF
THE
EGF-LIKE
DOMAINS
L-VIII
IS
A
LIGAND
FOR
ERBB-1
.
21
THE
ORPHAN
RECEPTOR
ERBB-2
BINDS
NONE
OF
THE
EGF-LIKE
MOTIFS
OF
PROEGF
.
26
NDF
RECEPTORS,
ERBB-3
AND
ERBB-4
DO
NOT
CROSSREACT
WITH
THE
PROEGF
MOTIFS
.
28
DISCUSSION
.
33
THE
EXPERIMENTAL
STRATEGY
.
34
LIGAND
CRITERIA
AND
RECEPTOR
BINDING
.
36
POSSIBLE
ADDITIONAL
FUNCTIONS
OF
PROEGF
.
40
MATERIALS
AND
METHODS
.
46
MATERIALS
.
46
GENERATION
OF
ANTIBODIES
AND
ELISA
.
46
CELL
CULTURE
.
48
PLASMID
CONSTRUCTIONS
.
49
PROTEIN
EXPRESSION
AND
PURIFICATION.
51
WESTERN
BLOTTING
AND
DETECTION
.
52
DIRECT
BINDING
EXPERIMENTS
.
53
DISPLACEMENT
ASSAYS
.
53
TYROSINE
PHOSPHORYLATION
ASSAYS
.
54
IMMUNOPRECIPITATION
.
54
PH-METHYL]THYMIDINE
INCORPORATION.
55
CELL
PROLIFERATION
AND
COLONY
FORMATION
.
55
MODEL
BUILDING
FOR
THE
STRUCTURE
PREDICTIONS
.
56
REFERENCES
.
59
APPENDIX
.
72 |
| any_adam_object | 1 |
| author | Glathe, Stefanie |
| author_facet | Glathe, Stefanie |
| author_role | aut |
| author_sort | Glathe, Stefanie |
| author_variant | s g sg |
| building | Verbundindex |
| bvnumber | BV010809302 |
| ctrlnum | (OCoLC)258525186 (DE-599)BVBBV010809302 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV010809302</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">19961105</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">960624s1996 ad|| m||| 00||| engod</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">948216875</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)258525186</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV010809302</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91</subfield><subfield code="a">DE-29T</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-83</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Glathe, Stefanie</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor</subfield><subfield code="c">vorgelegt von Stefanie Glathe</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1996</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">77 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Berlin, Freie Univ., Diss., 1996</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Struktur-Aktivitäts-Beziehung</subfield><subfield code="0">(DE-588)4183784-8</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Präkursor</subfield><subfield code="0">(DE-588)4300438-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Epidermaler Wachstumsfaktor</subfield><subfield code="0">(DE-588)4271160-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Epidermaler Wachstumsfaktor</subfield><subfield code="0">(DE-588)4271160-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Präkursor</subfield><subfield code="0">(DE-588)4300438-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Struktur-Aktivitäts-Beziehung</subfield><subfield code="0">(DE-588)4183784-8</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007221552&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-007221552</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV010809302 |
| illustrated | Illustrated |
| indexdate | 2024-08-14T01:16:03Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007221552 |
| oclc_num | 258525186 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-29T DE-12 DE-355 DE-BY-UBR DE-83 DE-11 DE-188 |
| owner_facet | DE-91 DE-BY-TUM DE-29T DE-12 DE-355 DE-BY-UBR DE-83 DE-11 DE-188 |
| physical | 77 S. Ill., graph. Darst. |
| publishDate | 1996 |
| publishDateSearch | 1996 |
| publishDateSort | 1996 |
| record_format | marc |
| spelling | Glathe, Stefanie Verfasser aut Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor vorgelegt von Stefanie Glathe 1996 77 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Berlin, Freie Univ., Diss., 1996 Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd rswk-swf Präkursor (DE-588)4300438-6 gnd rswk-swf Epidermaler Wachstumsfaktor (DE-588)4271160-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Epidermaler Wachstumsfaktor (DE-588)4271160-5 s Präkursor (DE-588)4300438-6 s Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007221552&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Glathe, Stefanie Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd Präkursor (DE-588)4300438-6 gnd Epidermaler Wachstumsfaktor (DE-588)4271160-5 gnd |
| subject_GND | (DE-588)4183784-8 (DE-588)4300438-6 (DE-588)4271160-5 (DE-588)4113937-9 |
| title | Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor |
| title_auth | Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor |
| title_exact_search | Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor |
| title_full | Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor vorgelegt von Stefanie Glathe |
| title_fullStr | Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor vorgelegt von Stefanie Glathe |
| title_full_unstemmed | Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor vorgelegt von Stefanie Glathe |
| title_short | Potential ligand functions of the multiple EGF-like domains of the human epidermal growth factor precursor |
| title_sort | potential ligand functions of the multiple egf like domains of the human epidermal growth factor precursor |
| topic | Struktur-Aktivitäts-Beziehung (DE-588)4183784-8 gnd Präkursor (DE-588)4300438-6 gnd Epidermaler Wachstumsfaktor (DE-588)4271160-5 gnd |
| topic_facet | Struktur-Aktivitäts-Beziehung Präkursor Epidermaler Wachstumsfaktor Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007221552&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT glathestefanie potentialligandfunctionsofthemultipleegflikedomainsofthehumanepidermalgrowthfactorprecursor |