Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
1995
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Text engl.. - Köln, Univ., Diss., 1996 |
| Beschreibung: | 101 S. Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Bauer, Petra |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa |c vorgelegt von Petra Bauer |
| 264 | 1 | |c 1995 | |
| 300 | |a 101 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Text engl.. - Köln, Univ., Diss., 1996 | ||
| 502 | |a Köln, Univ., Diss., 1995 | ||
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-007207788 | |
Datensatz im Suchindex
| _version_ | 1807502277591695360 |
|---|---|
| adam_text |
INDEX
1.
INTRODUCTION
.
1
1.1
MORPHOLOGICAL
ASPECTS
OF
NODULE
DEVELOPMENT
.
2
1.1.1.
SEQUENTIAL
EVENTS
OF
NODULE
FORMATION
AND
RHIZOBIUM
INFECTION
.
2
1.1.2.
MORPHOLOGY
OF
AN
INDETERMINATE
NODULE
.
3
1.1.3.
COMPARISON
BETWEEN
NODULE
AND
LATERAL
ROOT
DEVELOPMENT
.
4
1.2.
MOLECULAR
EVENTS
ASSOCIATED
WITH
NODULE
DEVELOPMENT
.
5
1.2.1.
RHIZOBIUM
SIGNAL
MOLECULES
REQUIRED
FOR
NODULE
INITIATION
AND
INVASION
.
5
1.2.1.1.
HOST-SPECIFIC
RHIZOBIUM
NOD
FACTORS
.
5
1.2.1.2.
RHIZOBIUM
OUTER
SURFACE
POLYSACCHARIDES
.
8
1.2.2.
PLANT
SIGNALS
REGULATING
NODULATION
AND
PLANT
EARLY
NODULIN
GENES
.
8
1.2.2.1.
ROLE
OF
PLANT
HORMONES
.
8
1.2.2.2.
PLANT
CONTROL
OF
NODULATION
.
9
I.2.2.3.
EARLY
NODULIN
GENE
EXPRESSION
.
10
1.3
USE
OF
EARLY
NODULIN
GENES
FOR
STUDYING
THE
NODULATION
PROCESS
.
12
2.
AIMS
.
13
3.
MATERIAL
.
15
3.1.
BIOLOGICAL
MATERIAL
.
15
3.1.1.
PLANT
MATERIAL
.
15
3.1.2.
BACTERIAL
STRAINS
.
15
3.1.3.
MYCORRHIZAL
INOCULUM
.
16
3.2.
DNA
MATERIAL
.
16
3.2.1.
PLASMIDS
.
16
3.2.2.
SYNTHETIC
OLIGONUCLEOTIDES
.
17
3.3.
GROWTH
MEDIA
.
18
3.3.1.
PLANT
GROWTH
MEDIA
.
18
3.3.2.
BACTERIAL
GROWTH
MEDIA
.
19
3.4.
GENERAL
BUFFERS
.
20
3.5.
CHEMICALS,
ENZYMES,
KITS
.
20
4.
METHODS
.
21
4.1
MOLECULAR
BIOLOGICAL
TECHNIQUES
.
21
4.1.1
STANDARD
METHODS
AND
COMPUTER
ANALYSIS
.
21
4.1.2.
REVERSE
TRANSCRIPTION-POLYMERASE
CHAIN
REACTION
(RT-PCR)
GENE
EXPRESSION
ANALYSIS
.
21
4.1.2.1.
TOTAL
RNA
PREPARATION
AND
DNASE
I-TREATMENT
.
21
4.1.2.2.
RT-PCR
.
22
4.I.2.3.
GEL
ELECTROPHORESIS
AND
SOUTHERN
BLOT
.
22
4.1.3.
PLASMID
CONSTRUCTION
.
23
4.1.3.1.
GENERATION
OF
PROMOTER-GUSA
FUSIONS
IN
BINARY
VECTORS
.
23
4.1.3.2.
SUBCLONING
OF
PROMOTER-GUSA
FUSIONS
INTO
PBLUESCRIPT
.
23
4.2.
GENE
TRANSFER
.
25
4.2.1.
RHIZOBIUM
AND
AGROBACTERIUM
-
TRIPARENTAL
CONJUGATION
.
25
4.2.2.
TRANSFORMATION
AND
REGENERATION
OF
M.
SATIVA
SSP.
VARIA LINE
A2
.
25
4.2.2.1.
TRANSFORMATION-REGENERATION
PROCEDURE
.
25
4.2.2.2.
PLANT
GENOMIC
DNA
PREPARATION
AND
SOUTHERN
BLOT
.
25
4.3.
PLANT
GROWTH
AND
PHYSIOLOGICAL
ASSAYS
.
26
4.3.1.
GENERATION
OF
PLANT
MATERIAL
FOR
RT-PCR
STUDIES
.
26
4.3.2.
PLANT
MATERIAL
FOR
HISTOCHEMICAL
GUS
STAININGS
.
27
4.4.
HISTOCHEMICAL
LOCALIZATION
OF
GUS
ACTIVITY
.
28
4.4.1.
PREFIXATION
AND
GUS
ASSAYS
.
28
4.4.2.
CLEARING
OF
WHOLE
ROOTS,
SEMITHIN
SECTIONING
AND
MICROSCOPIC
OBSERVATIONS
.
28
4.4.3.
SPECTROCOLORIMETRIC
GUS
ASSAYS
.
29
4.5.
MICROPROJECTILE
BOMBARDMENT
.
30
4.5.1.
PARTICLE
PREPARATION
.
30
4.5.2.
PREPARATION
OF
THE
PLANT
MATERIAL
.
30
4.5.3.
PARTICLE
INFLOW
GUN
-
TRANSIENT
EXPRESSION
.
30
4.5.4.
MICROTARGETING
-
TRANSIENT
EXPRESSION
.
31
5.
RESULTS
.
33
5.1.
CHARACTERIZATION
OF
MSENODL2A
AND
MSENODL2B
SEQUENCES
.
33
5.1.1.
SEQUENCE
COMPARISONS
BETWEEN
ENODL2
SEQUENCES
.
33
5.1.2.
COMPARISON
OF
THE
MSENODL2A
AND
MSENODL2B
PROMOTERS
.
36
5.2.
EXPRESSION
ANALYSIS
OF
MSENODL2A
AND
MSENODL2B
USING
REVERSE
TRANSCRIPTION-PCR
.
36
5.2.1.
ESTABLISHMENT
OF
A
MULTIPLE
TRANSCRIPT
RT-PCR
APPROACH
.
36
5.2.2.
MSENODL2A
AND
MSENODL2B
ARE
DIFFERENTIALLY
EXPRESSED
IN
VARIOUS
PLANT
ORGANS
.
37
5.2.3.
AFTER
RHIZOBIUM
MELILOTI
INOCULATION
MSENODL2B
IS
EARLIER
INDUCED
THAN
MSENODL2A
.
38
5.2.4.
MSENODL2B
BUT
NOT
MSENODL2A
EXPRESSION
IS
ENHANCED
AFTER
NOD
FACTOR
APPLICATION
TO
ROOTS
.
39
5.2.5.
MSENODL2A
AND
MSENODL2B
EXPRESSION
IN
NODULES
WITH
DIFFERENT
DEGREES
OF
RHIZOBIUM
INVASION
.
41
5.3.
GENERATION
OF
TRANSGENIC
ALFALFA
CONTAINING
MSENODL2A
AND
MSENODL2B
PROMOTER-GUSA
FUSIONS
.
42
5.4.
SPATIAL
EXPRESSION
PATTERNS
OF
MSENODL2A
DURING
SYMBIOSIS
AND
PLANT
DEVELOPMENT
.
45
5.4.1.
AFTER
RHIZOBIUM
INOCULATION
AND
NOD
FACTOR
TREATMENT
MSENODL2A
EXPRESSED
FIRST
IN
THE
DIVIDING
CORTICAL
CELLS
OF
THE
ROOT
.
46
5.4.2.
BACTERIAL
OUTER
SURFACE
COMPONENTS
DO
NOT
REPRESENT
SIGNALS
FOR
MSENODL2A
INDUCTION
.
48
5.4.3.
MSENOD]2A
EXPRESSION
IS
ASSOCIATED
WITH
NODULE
ORGANOGENESIS,
BUT
NOT
WITH
RHIZOBIUM
INVASION
.
49
5.4.4.
MSENODL2A
EXPRESSION
DURING
LATERAL
AND
ADVENTITIOUS
ROOT
FORMATION
.
54
5.4.5.
LOW
MSENODL2A
EXPRESSION
IN
SOMATIC
EMBRYOS
AND
AT
FLOWER
BUD
AXILS
.
56
5.5.
USE
OF
THE
MSENODL2A-GUSK
FUSION
AS
MOLECULAR
MARKER
FOR
STUDYING
THE
ACTION
OF
PLANT
HORMONES
AND
GROWTH
REGULATORS
IN
NODULE
INITIATION
.
57
5.5.1.
ACTION
OF
AUXIN
TRANSPORT
INHIBITORS
ON
ALFALFA
ROOTS
.
58
5.5.1.1.
THE
AUXIN
TRANSPORT
INHIBITOR
NPA
INDUCES
COMPLEX
MORPHOLOGICAL
CHANGES
ON
ALFALFA
ROOTS
INCLUDING
THE
INDUCTION
OF
CORTICAL
CELL
DIVISIONS
.
58
5.5.1.2.
PLANT
FLAVONOIDS
DO
NOT
AFFECT
GUS
STAINING
PATTERNS
AS
OBSERVED
AFTER
NPA
TREATMENT
.
60
5.5.2.
CYTOKININ
ACTION
ON
ALFALFA
ROOTS
.
60
5.5.2.1.
CYTOKININS
INDUCE
SIMILAR
PATTERNS
OF
CORTICAL
CELL
DIVISION,
MSENODL2A
EXPRESSION
AND
AMYLOPLAST
DEPOSITION
AS
NOD
FACTORS
.
60
5.5.2.2.
CYTOKININ
ACTION
ON
ALFALFA
ROOTS
IS
CONTROLLED
BY
PLANT
FACTORS
PRESUMABLY
ARISING
FROM
THE
ALTERED
CARBON/NITROGEN
METABOLISM
DURING
NITROGEN
STARVATION
.
64
5.5.3.
AUXINS
INDUCE
TYPICAL
PATTERNS
OF
MSENODL2A
EXPRESSION
DISTINCT
FROM
THOSE
OBSERVED
AFTER
CYTOKININ
AND
NOD
FACTOR
TREATMENT
.
67
5.6.
MICROPROJECTILE
BOMBARDMENT
OF
ALFALFA
ROOTS
FOR
TRANSIENT
EXPRESSION
.
69
5.6.1.
INFLUENCE
OF
PRE
AND
POSTPLASMOLYSIS
ON
TRANSFORMATION
FREQUENCIES
.
70
5.6.2.
COMPARISON
OF
TRANSFORMATION
FREQUENCIES
BETWEEN
BEAN
AND
ALFALFA
ROOTS
.
72
5.6.3.
TRANSIENT
EXPRESSION
ASSAYS
ON
NODULES
.
72
5.3.4.
MICROSCOPIC
ANALYSIS
OF
THE
MICROTARGETED
ROOTS
.
73
6.
DISCUSSION
.
74
6.1.
EVALUATION
OF
TRANSGENIC
REPORTER
GENE
SYSTEMS
FOR
THE
ANALYSIS
OF
GENE
EXPRESSION
PATTERNS
.
74
6.2.
IN
ALFALFA,
ENODL2
GENES
ARE
DIFFERENTIALLY
REGULATED
DURING
SYMBIOSIS
AND
PLANT
DEVELOPMENT
.
75
6.3.
SIGNALS
INVOLVED
IN
MSENODL2A
AND
MSENODL2B
INDUCTION
AND
PUTATIVE
ROLES
OF
THE
GENE
PRODUCTS
IN
NODULATION
.
77
6.4.
MSENODL2B
AS
MOLECULAR
MARKER
TO
STUDY
EARLY
NOD
FACTOR
RESPONSES
AT
THE
ROOT
SURFACE
.
79
6.5.
MSENODL2A
AS
MOLECULAR
MARKER
TO
STUDY
CORTICAL
CELL
RESPONSES
TO
NOD
FACTORS
-
COMMON
ELEMENTS
IN
NOD
FACTOR
AND
CYTOKININ
SIGNALLING
.
80
6.6.
DURING
NODULE
FORMATION
CYTOKININS
MAY
BE
INVOLVED
IN
COORDINATING
METABOLIC
ACTIVITIES
WITH
ORGANOGENESIS
.
82
6.7.
EARLY
NODULIN
GENE
EXPRESSION
INDICATES
THAT
NODULES
ARE
MODIFIED
LATERAL
ROOT
STRUCTURES
.
84
7.
PERSPECTIVES
.
85
8.
LITERATURE
.
87
9.
ANNEX
.
97
9.1.
ABBREVIATIONS
.
97
9.2.
ACKNOWLEDGEMENTS
.
99
9.3.
LEBENSLAUF
.
100
9.4.
PUBLIKATIONEN
.
101 |
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| author | Bauer, Petra |
| author_facet | Bauer, Petra |
| author_role | aut |
| author_sort | Bauer, Petra |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV010790301 |
| illustrated | Illustrated |
| indexdate | 2024-08-16T00:34:58Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007207788 |
| oclc_num | 55069278 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-83 DE-11 DE-188 |
| owner_facet | DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-83 DE-11 DE-188 |
| physical | 101 S. Ill., graph. Darst. |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| record_format | marc |
| spelling | Bauer, Petra Verfasser aut Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa vorgelegt von Petra Bauer 1995 101 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Text engl.. - Köln, Univ., Diss., 1996 Köln, Univ., Diss., 1995 Luzerne (DE-588)4168357-2 gnd rswk-swf Gen (DE-588)4128987-0 gnd rswk-swf Wurzelknöllchenbildung (DE-588)4240778-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Luzerne (DE-588)4168357-2 s Wurzelknöllchenbildung (DE-588)4240778-3 s Gen (DE-588)4128987-0 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007207788&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bauer, Petra Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa Luzerne (DE-588)4168357-2 gnd Gen (DE-588)4128987-0 gnd Wurzelknöllchenbildung (DE-588)4240778-3 gnd |
| subject_GND | (DE-588)4168357-2 (DE-588)4128987-0 (DE-588)4240778-3 (DE-588)4113937-9 |
| title | Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa |
| title_auth | Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa |
| title_exact_search | Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa |
| title_full | Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa vorgelegt von Petra Bauer |
| title_fullStr | Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa vorgelegt von Petra Bauer |
| title_full_unstemmed | Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa vorgelegt von Petra Bauer |
| title_short | Charakterisierung früher pflanzlicher Nodulingene in Medicago sativa |
| title_sort | charakterisierung fruher pflanzlicher nodulingene in medicago sativa |
| topic | Luzerne (DE-588)4168357-2 gnd Gen (DE-588)4128987-0 gnd Wurzelknöllchenbildung (DE-588)4240778-3 gnd |
| topic_facet | Luzerne Gen Wurzelknöllchenbildung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007207788&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT bauerpetra charakterisierungfruherpflanzlichernodulingeneinmedicagosativa |