Neural cell culture: a practical approach
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Oxford [u.a.]
IRL Press
1995
|
| Schriftenreihe: | The practical approach series
165 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XX, 248 S. Ill., graph. Darst. |
| ISBN: | 0199634858 019963484X |
Internformat
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Datensatz im Suchindex
| _version_ | 1804125195620843520 |
|---|---|
| adam_text | Neural Cell Culture
A Practical Approach
Edited by
JAMES COHEN
Division of Anatomy and Cell Biology,
UMDS - Guy s Campus, London Bridge, London SEl 9RT
and
GRAHAMPWILKIN
Department of Biochemistry,
Imperial College of Science, Technology and Medicine,
Kensington, London SW7 2AZ
OXFORD UNIVERSITY PRESS
Oxford New York Tokyo
Contents
List of contributors
Abbreviations
I CNS neurons 1
1 Cerebellar Granule cells 3
Martin A Cambray-Deakin
1 Introduction 3
2 Initial preparation 3
Stock solutions 3
Buffers and media for cell culture 4
3 Granule cells in culture 11
References 13
2 Cerebellar Purkinje neurons 15
Susana Cohen-Cory
1 Introduction 15
2 Nutrient media 16
Nutrient medium supplemented with serum 16
Chemically defined, serum-free medium 17
3 Dissection and tissue preparation 18
Optimal stage for growing Purkinje neurons 18
Removal of embryos 19
Cerebellar dissection from embryonic rat 19
4 Tissue dissociation, plating densities, and conditions for cell
growth 20
Mechanical and enzymatic tissue dissocation 20
Determination of cell yields and plating density 22
Conditions for cell growth 22
5 Identification of Purkinje neurons 22
Acknowledgements 24
References 24
Contents
3 Nigral and striatal neurons 25
Roger Barker and Alan Johnson
1 Introduction 25
2 Equipment 25
3 Solutions and reagents 28
4 Nigral cultures 29
5 Striatal cultures 36
6 Concluding remarks 36
Acknowledgements 39
References 39
4 Septal neurons 41
Clive Svendsen
1 Introduction 41
2 Standard techniques for culturing, staining, and counting
septal neurons 42
The sandwich technique 42
3 Quantitative and qualitative analysis of septal cultures 46
Cell counting and area measurements 46
Appearance of sandwich cultures and comparison with traditional
methods 47
Staining methods to identify neurons and glia 48
4 Variables that affect neuronal survival using the sandwich
technique 51
References 52
5 Proliferation and differentiation in retinal cell
cultures 55
Laura Lillien
1 Introduction 55
Retinal development in vivo 55
Use of in vitro systems to study the control of retinal development 56
2 Culture preparation 57
Culture media 57
Dissection of retina 58
Minimal systems: monolayer and collagen gel cultures 60
Maximal systems: aggregate and explant cultures 61
Contents
3 Use of retroviruses expressing lineage markers
4) Differentiation
Cell type markers
Lineage markers
Lamination
Transplantation in vitro
References
6 Purified embryonic motoneurons 69
Christopher E Henderson, Evelyne Bloch-Gallego, and
William Camu
1 Introduction 69
2 Purification strategy 70
Choice of species 70
Choice of embryo age 70
Choice of purification method 71
3 Experimental methods 72
Acknowledgements 80
References 81
II CNS glia and non-neural cells 83
7 Astrocytes 85
Derek R Marriott, Warren D Hirst, and M Cecilia Ljungberg
1 Introduction 85
2 Mixed primary glial cultures 86
3 Mixed primary astroglial cultures from adult tissue 91
4 Preparation of type-2 astrocytes 93
Acknowledgements 95
References 96
8 Oligodendrocyte lineage cells from neonatal
rat brain 97
Ellen J Collarini
1 Introduction 97
xi
Contents
2 Establishing mixed glial cultures 98
3 Isolation of oligodendrocyte precursor cells from mixed glial
cultures 100
Acknowledgements 103
References 105
9 Microglia: the tissue macrophage of the CNS 107
M Nicola Woodroofe and M Louise Cuzner
1 General introduction 107
2 Isolation of microglia 108
Introduction 108
Dissociation of CNS tissue 109
Separation of microglia 109
Advantages and disadvantages of the methods 114
Cell yields 114
Culture media 114
3 Cellular markers for microglia 115
4 Microglial morphology 117
Acknowledgements 118
References 118
10 Brain endothelium 121
David Male
1 Introduction 121
2 Isolation of capillary fragments 121
General considerations 121
3 Growth conditions 123
Substrata 123
Growth media and conditions 124
Passaging the cells 126
4 Phenotyping 127
5 Problems 128
6 CNS endothelium from other species 129
References 129
xii
Contents
III PNS neurons and glia 131
11 Neural crest cells: strategies to generate
lineage diversification in vitro 133
Jean-Loup Duband, Muriel Delannet, and Frederique Monier
1 Introduction 133
2 Primary culture of neural crest cells 136
Truncal neural crest 136
Chick cranial neural crest 143
3 Establishing conditions for differentiation of neural crest
cells in vitro 146
Cloning neural crest cells 146
Factors affecting neural crest cell differentiation 147
Markers of neural crest cell differentiation 149
Acknowledgements 151
References 151
12 Cranial sensory neurons 153
Alun M Davies
1 Introduction 153
2 Dissection techniques 153
Early cranial sensory ganglia from chicken embryos 155
Mid-embryonic cranial sensory neurons from chicken embryos 156
Trigeminal ganglia from mouse embryos - 161
Nodose ganglia from mouse embryos 164
3 Dissociation of dissected neural tissue 165
4 Separation of neurons from non-neuronal cells 168
Differential adhesion 169
Gravity sedimentation 169
5 Culturing isolated neurons 170
Culture substratum 170
Preparation of the culture medium 171
Seeding the neurons 173
Neuronal survival 173
Acknowledgements 174
References 174
xiii
Contents
13 Avian ciliary and lumbar sympathetic
ganglion neurons 177
Rae Nishi
1 Introduction 177
2 Dissection of ciliary and lumbar sympathetic ganglia 178
Comments 178
Suggested age of embryos to use for dissections 178
3 Dissociation of ganglia 181
Comments 181
4 Culturing ciliary and sympathetic ganglia 185
Comments 185
Medium 185
Reduction of the number of non-neuronal cells 187
References 188
14 Enteric ganglion cells 189
M Jill Saffrey
1 Introduction 189
2 Problems in the isolation of intramural ganglia 191
3 Dissection of intact enteric ganglia from the gut wall 191
Myenteric ganglia 191
Submucous plexus ganglia 196
4 Explant cultures of isolated ganglia 196
Culture chambers, substrates, and media 196
Method of explantation 197
Guinea-pig myenteric plexus explants 197
Rat myenteric plexus explants 199
Guinea-pig submucous plexus explants 199
5 Purified populations of enteric ganglion cells 199
Enteric neurons 199
Enteric glial cells 200
6 Dissociated cell cultures of postnatal enteric ganglion cells 201
Dissociation of isolated enteric ganglia 201
Acknowledgements 202
References 202
15 Mouse olfactory epithelium 205
Anne L Calof, Jose L Guevara, and Melinda K Gordon
1 Introduction 205
xiv
Contents
2 Dissection and purification of olfactory epithelium
:• from mouse embryos 206
Dissection 206
3 Culturing olfactory epithelium explants 209
Preparation of substrata for explant cultures 210
Culture media 211
Culturing explants 211
Growth factor requirements 213
4 Culture of isolated neurons and neuronal precursors 213
Preparation of substrata for dissociated neuronal cell fraction 214
Suspension culture and isolation of the neuronal cell fraction 215
5 Analysis of cell types within olfactory epithelium cultures 217
Olfactory receptor neuron markers 217
Olfactory Schwann cell (ensheathing cell) markers 217
Sustentacular cell marker 218
Basal cell marker 218
Acknowledgements 218
References 218
16 Schwann cell culture 221
Louise Morgan
1 Introduction 221
2 Nerve dissociation 221
Desheathing 222
Dissociation 225
Schwann cell yield 226
3 Maintaining Schwann cells in vitro 227
Schwann cell purification 227
4 Alternative methods for culturing Schwann cells 229
5 Manipulation of Schwann cells in vitro 232
Schwann cell mitogens 232
Use of mitogens to expand Schwann cell numbers 232
6 Schwann cell phenotype in vitro 234
7 Schwann cells from various species 234
8 Transformed Schwann cells and schwannoma cell lines 236
References 236
Index 239
XV
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| spelling | Neural cell culture a practical approach ed. by James Cohen ... Oxford [u.a.] IRL Press 1995 XX, 248 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier The practical approach series 165 Neurones - Croissance - Manuels de laboratoire ram Neurones - Cultures et milieux de culture - Manuels de laboratoire ram Zellkultur (DE-588)4067547-6 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Nervenzelle (DE-588)4041649-5 gnd rswk-swf Nervenzelle (DE-588)4041649-5 s Zellkultur (DE-588)4067547-6 s Methode (DE-588)4038971-6 s DE-604 Cohen, James Sonstige oth The practical approach series 165 (DE-604)BV007921321 165 HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007155074&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Neural cell culture a practical approach The practical approach series Neurones - Croissance - Manuels de laboratoire ram Neurones - Cultures et milieux de culture - Manuels de laboratoire ram Zellkultur (DE-588)4067547-6 gnd Methode (DE-588)4038971-6 gnd Nervenzelle (DE-588)4041649-5 gnd |
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| title | Neural cell culture a practical approach |
| title_auth | Neural cell culture a practical approach |
| title_exact_search | Neural cell culture a practical approach |
| title_full | Neural cell culture a practical approach ed. by James Cohen ... |
| title_fullStr | Neural cell culture a practical approach ed. by James Cohen ... |
| title_full_unstemmed | Neural cell culture a practical approach ed. by James Cohen ... |
| title_short | Neural cell culture |
| title_sort | neural cell culture a practical approach |
| title_sub | a practical approach |
| topic | Neurones - Croissance - Manuels de laboratoire ram Neurones - Cultures et milieux de culture - Manuels de laboratoire ram Zellkultur (DE-588)4067547-6 gnd Methode (DE-588)4038971-6 gnd Nervenzelle (DE-588)4041649-5 gnd |
| topic_facet | Neurones - Croissance - Manuels de laboratoire Neurones - Cultures et milieux de culture - Manuels de laboratoire Zellkultur Methode Nervenzelle |
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