Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
1995
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Göttingen, Univ., Diss., 1995 |
| Beschreibung: | IX, 148 S. Ill., graph. Darst. |
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| 100 | 1 | |a Bdliya, Bulus Shapshi |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers |c by Bulus Shapshi Bdliya |
| 264 | 1 | |c 1995 | |
| 300 | |a IX, 148 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Göttingen, Univ., Diss., 1995 | ||
| 650 | 0 | 7 | |a Knollennassfäule |0 (DE-588)4385357-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Nachweis |0 (DE-588)4115334-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Kartoffel |0 (DE-588)4029804-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Erwinia |0 (DE-588)4152948-0 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Erwinia |0 (DE-588)4152948-0 |D s |
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| 689 | 1 | 1 | |a Knollennassfäule |0 (DE-588)4385357-2 |D s |
| 689 | 1 | 2 | |a Erwinia |0 (DE-588)4152948-0 |D s |
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Datensatz im Suchindex
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| adam_text |
CONTENTS
E3GS
1
INTRODUCTION
1
2
MATERIALS
AND
METHODS
3
2.1
MEDIA,
REAGENTS
AND
CHEMICALS
3
2.1.1
MEDIA
FOR
BACTERIA
3
2.1.2
REAGENTS,
BUFFERS
AND
CHEMICALS
FOR
IMMUNOLOGICAL
AND
SEROLOGICAL
TESTS
7
2.1.2.1
STOCK
SOLUTIONS
FOR
SODIUM
DODECYL
SULPHATE
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
(SDS-PAGE)
7
2.1.2.2
ELECTROPHORESIS
GELS
8
2.1.2.3
BUFFER
FOR
PREPARATION
OF
BACTERIAL
PROTEINS
8
2.1.2.4
COOMASSIE
BLUE
STAINING
SOLUTIONS
9
2.1.2.5
SOLUTIONS
FOR
WESTERN
BLOT
9
2.1.3
REAGENTS
AND
BUFFERS
FOR
ELISA
11
2.1.4
BUFFERS
FOR
THE
IMMUNOFLUORESCENCE
TEST
(IF
TEST)
13
2.1.5
REAGENTS
AND
BUFFERS
FOR
THE
POLYMERASE
CHAIN
REACTION
(PCR)
14
2.1.5.1
SOLUTIONS
AND
BUFFERS
FOR
THE
AGAROSE
GEL
ELECTROPHORESIS
15
2.1.6
ANTIBIOTICS
USED
IN
THIS
STUDY
16
2.1.6.1
OTHER
REAGENTS
16
2.1.7
ANTISERA
USED
IN
THIS
STUDY
17
2.1.8
STATISTICAL
ANALYSIS
17
2.2
BACTERIA
18
2.2.1
ISOLATION
AND
IDENTIFICATION
18
2.2.2
MAINTENANCE
AND
STORAGE
OF
BACTERIAL
CULTURES
18
2.2.3
CONVENTIONAL
PHYSIOLOGICAL
TESTS
18
2.2.3.1
GRAM
STAINING
18
2.2.3.2
UTILISATION
OF
A-METHYL-D-GLUCOSIDE
19
2.2.3.3
REDUCING
SUBSTANCES
FROM
SUCROSE
-
19
2.2.3.4
PHOSPHATASE
PRODUCTION
19
2.2.3.5
INDOLE
PRODUCTION
19
IT
2.3
DEVELOPMENT
OF
A
SEMI-SELECTIVE
MEDIUM
FOR
ISOLATION
OF
ERWINIA
SPECIES
21
2.3.1
EFFECT
OF
TRYPTONE
IN
THE
OVER-LAYER
ON
THE
GROWTH
AND
CAVITY
FORMATION
BY
ERWINIA
SPP.
ON
THE
SEMI-SELECTIVE
MEDIUM
21
2.3.1.1
EVALUATION
OF
BACTERIAL
GROWTH
ON
THE
SEMI-SELECTIVE
MEDIUM
22
2.3.2
EFFECT
OF
BASAL
MEDIUM
ON
THE
SENSITIVITY
OF
THE
SEMI-SELECTIVE
MEDIUM
22
2.3.3
EFFECT
OF
ANTIBIOTICS
ON
THE
GROWTH
OF
ERWINIA
SPP.
22
2.3.4
EFFECT
OF
POLYMYXIN
B
SULPHATE
AND
CHLORAMPHENICOL
ON
GROWTH
AND
CAVITY
FORMATION
BY
ERWINIA
SPP.
ON
THE
SEMI-SELECTIVE
MEDIUM
22
2.3.5
COMPARISON
BETWEEN
THE
NEWLY
DEVELOPED
SEMI-SELECTIVE
MEDIUM
(CVPD)
AND
CVPB
MEDIUM
FOR
DETECTION
OF
ERWINIA
SPP.
FROM
ARTIFICIALLY
INOCULATED
POTATO
TUBERS
23
2.4
DEVELOPMENT
OF
A
SEMI-SELECTIVE
MEDIUM
FOR
ISOLATION
OF
ERWINIA
CAROTOVORA
SUBSP
CAROTOVORA
(ECC)
23
2.5
PRODUCTION
OF
MONOSPECIFIC
POLYCLONAL
ANTISERUM
AGAINST
ECA
STRAINS
AND
ITS
SEROLOGICAL
APPLICATIONS
24
2.5.1
POLYCLONAL
ANTISERA
RAISED
AGAINST
ECA
STRAINS
USED
IN
THIS
EXPERIMENT
24
2.5.2
SEPARATION
OF
BACTERIAL
PROTEINS
24
2.5.2.1
PREPARATION
OF
BACTERIAL
PROTEINS
24
2.5.2.2
SDS-PAGE
24
2.5.2.3
ELECTROBLOTTING
25
2.5.2.4
IMMUNOBLOTTING
25
2.5.2.5
SELECTION
OF
SPECIFIC
PROTEINS
OF
ECA
STRAINS
26
2.5.2.6
EVALUATION
OF
THE
SPECIFICITY
OF
THE
ANTIBODIES
TO
THE
SELECTED
PROTEIN
BAND
26
2.5.2.7
PRODUCTION
OF
POLYCLONAL
ANTIBODIES
AGAINST
THE
SPECIFIC
PROTEINS
OF
ECA
STRAINS
26
2.5.2.8
EXTRACTION
AND
PURIFICATION
OF
THE
ANTISERUM
27
2.5.2.9
SEROLOGICAL
TESTS
27
2.6
DEVELOPMENT
OF
A
METHOD
FOR
EXTRACTION
OF
ELECTROPHORESED
PROTEINS
FROM
SDS-POLYACRYLAMIDE
GELS
28
2.7
ENZYME-LINKED
IMMUNOSORBENT
ASSAY
(ELISA)
28
2.7.1
INDIRECT
ELISA
28
2.7.2
DIRECT
ELISA
(DAS
ELISA)
29
ILL
2.7.3
CROSS
REACTION
TESTS
29
2.7.4
DETERMINATION
OF
THE
DETECTION
LIMIT
OF
INDIRECT
/
DIRECT
ELISA
METHOD
30
2.7.5
EFFECT
OF
POTATO
EXTRACT
ON
THE
DETECTION
LIMIT
OF
INDIRECT
ELIS
A
30
2.7.6
DETECTION
OF
ECA
FROM
ARTIFICIALLY
INOCULATED
POTATO
TUBERS
USING
INDIRECT
ELISA
30
2.7.7
EFFECT
OF
LIQUID
ENRICHMENT
MEDIUM
(LEM)
ON
THE
DETECTION
LIMIT
OF
THE
INDIRECT
ELISA
TEST
3
0
2.8
IMMUNOFLUORESCENCE
TEST
(IF
TEST)
31
2.8.1
DETERMINATION
OF
THE
DETECTION
LIMIT
OF
THE
IF
TEST
31
2.8.2
DETECTION
OF
ECA
FROM
ARTIFICIALLY
INOCULATED
POTATO
TUBERS
USING
THE
IF
TEST
32
2.9
POLYMERASE
CHAIN
REACTION
(PCR)
32
2.9.1
ANALYSIS
OF
THE
PCR
PRODUCTS
USING
AGAROSE
GEL
ELECTROPHORESIS
33
2.9.2
CROSS
REACTION
TEST
33
2.9.3
DETERMINATION
OF
THE
DETECTION
LIMIT
OF
THE
PCR
METHOD
34
2.9.4
DETECTION
OF
ECA
FROM
ARTIFICIALLY
INOCULATED
POTATO
TUBERS
34
2.10
EVALUATION
OF
TUBER
INCUBATION
METHODS
FOR
DETECTION
OF
SOFT
ROT
ERWINIA
SPP.
ON
POTATO
TUBERS
34
2.11
EFFECT
OF
INCUBATION
TEMPERATURE
AND
PERIOD
ON
DETECTION
RATE
OF
SOFT
ROT
ERWINIA
SPP.
ON
POTATO
TUBERS
INCUBATED
SUBMERGED
IN
STERILE
TAP
WATER
IN
AIR-TIGHT
JARS
35
2.12
COMPARISON
BETWEEN
INCUBATION
OF
TUBERS
IN
AIR-TIGHT
JARS
AND
WET
PAPER
TOWEL
INCUBATION
METHOD
FOR
DETECTION
OF
ERWINIA
SPP.
ON/
IN
POTATO
TUBERS
35
2.13
DETECTION
AND
IDENTIFICATION
OF
PECTOLYTIC
ERWINIA
SPP.
ON
TUBERS
OF
DIFFERENT
POTATO
VARIETIES
OF
DIFFERENT
ORIGINS
35
2.14
EFFECT
OF
TRITON
X
100
AND
TWEEN
80
(SURFACTANTS)
ON
THE
VIRULENCE
OF
ERWINIA
SPP.
36
2.15
SCREENING
POTATO
VARIETIES
FOR
TUBER
RESISTANCE
TO
SOFT
ROT
DISEASE
CAUSED
BY
ERWINIA
SPP.
36
2.15.1
SINGLE
SITE
METHOD
36
2.15.2
VACUUM
INFILTRATION
METHOD
37
2.15.3
POTATO
VARIETAL
SCREENING
FOR
TUBER
RESISTANCE
TO
SOFT
ROT
DISEASE
37
IV
2.15.4
EFFECT
OF
TRITON
X
100
ON
THE
SCREENING
OFPOTATO
VARIETIES
FOR
TUBER
RESISTANCE
TO
SOFT
ROT
DISEASE
37
2.16
GREEN-HOUSE
EXPERIMENTS
38
2.16.1
EVALUATION
OF
THE
VIRULENCE
OF
ERWINIA
SPP.
(LABORATORY
AND
GREEN-HOUSE
EXPERIMENTS)
38
2.16.2
EFFECT
OF
TEMPERATURE
ON
THE
VIRULENCE
OF
ERWINIA
SPP.
38
2.16.3
EFFECT
OF
INOCULUM
CONCENTRATION
ON
TUBER
SEED
PIECE
ROT,
EMERGENCE
AND
STEM
ROT
DISEASE
DEVELOPMENT
39
3
RESULTS
40
3.1
PRESERVATION
OF
THE
ERWINIA
SPECIES
USED
IN
THIS
STUDY
40
3.2
TESTS
FOR
IDENTIFICATION
OF
SOFT
ROT
ERWINIAS
(ECC,
ECA
AND
ECH)
40
3.3
DEVELOPMENT
OF
A
SEMI-SELECTIVE
MEDIUM
FOR
ISOLATION
OF
PECTOLYTIC
ERWINIA
SPECIES
43
3.3.1
EFFECT
OF
TRYPTONE
ON
THE
GROWTH
AND
CAVITY
FORMATION
BY
ERWINIA
SPP.
ON
THE
SEMI-SELECTIVE
MEDIUM
PREPARED
WITH
BULMER
SODIUM
POLYPECTATE
43
3.3.2
EFFECT
OF
BASAL
MEDIUM
ON
THE
SENSITIVITY
OF
THE
SEMI-SELECTIVE
MEDIUM
43
3.3.3
EFFECT
OF
DIFFERENT
ANTIBIOTICS
ON
THE
GROWTH
OF
ERWINIA
SPP.
45
3.3.4
EFFECT
OF
POLYMYXIN
B
SULPHATE
AND
CHLORAMPHENICOL
IN
THE
BASAL
MEDIUM
ON
THE
SENSITIVITY
OF
THE
SEMI-SELECTIVE
MEDIUM
45
3.3.5
EFFECT
OF
POLYMYXIN
B
SULPHATE
AND
CHLORAMPHENICOL
IN
THE
PECTATE
-
OVER-LAYER
ON
THE
SENSITIVITY
OF
THE
SEMI-SELECTIVE
MEDIUM
48
3.3.6
EVALUATION
OF
THE
SENSITIVITY
OF
THE
SEMI-SELECTIVE
MEDIUM
(CVPD)
50
3.3.7
DETECTION
OF
PECTOLYTIC
ERWINIA
SPP.
FROM
NATURALLY
CONTAMINATED
POTATO
TUBERS
51
3.3.8
COMPOSITION
OF
THE
NEW
SEMI-SELECTIVE
MEDIUM
(CVPD)
FOR
ISOLATION
OF
SOFT
ROT
ERWINIAS
52
3.3.9
COMPARISON
BETWEEN
CVPB
AND
CVPD
SELECTIVE
MEDIA
FOR
RECOVERY
OF
ERWINIA
SPP.
FROM
ARTIFICIALLY
INOCULATED
POTATO
TUBER
SLICES
53
3.3.10
DETERMINATION
OF
THE
DETECTION
LIMIT
OF
THE
CVPD
SEMI-SELECTIVE
MEDIUM
56
3.3.11
DEVELOPMENT
OF
A
MEDIUM
FOR
THE
SELECTIVE
ISOLATION
OF
ERWINIA
CAROTOVORA
SUBSP.
CAROTOVORA
(ECC)
56
3.3.11.1
EFFECT
OF
ANTIBIOTICS
ON
THE
GROWTH
OF
SOFT
ROT
ERWINIA
SPECIES
56
3.3.11.2
COMPOSITION
OF
THE
SEMI-SELECTIVE
MEDIUM
FOR
ISOLATION
OF
ECC
59
3.4
PRODUCTION
OF
MONOSPECIFIC
POLYCLONAL
ANTISERUM
(MPA)
AGAINST
ERWINIA
CAROTOVORA
SUBSP.
ATROSEPTICA
(ECA)
61
3.4.1
SEPARATION
OF
THE
BACTERIAL
PROTEIN
BY
SODIUM
DODECYL
SULPHATE
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
(SDS-PAGE)
61
3.4.2
SELECTION
OF
A
PROTEIN
SPECIFIC
TO
ECA
STRAINS
62
3.4.3
EVALUATION
OF
THE
SPECIFICITY
OF
THE
MONOSPECIFIC
POLYCLONAL
ANTISERUM
RAISED
AGAINST
THE
43
KDA
PROTEIN
OF
ECA
STRAINS
62
3.4.4
DETECTION
OF
ERWINIA
SPP.
USING
WESTERN
BLOT
WITH
ECA
MPA
65
3.5
DEVELOPMENT
OF
A
METHOD
FOR
EXTRACTION
OF
ELECTROPHORESED
PROTEINS
FROM
SDS-POLYACRYLAMIDE
GELS
65
3.6
THE
DETECTION
OF
ERWINIA
SPP.
USING
INDIRECT
ELISA
67
3.6.1
THE
DETECTION
LIMIT
OF
THE
INDIRECT
ELISA
TEST
USING
ECA
POLYCLONAL
AND
MONOSPECIFIC
POLYCLONAL
(MPA)
ANTISERA
70
3.6.2
THE
DETECTION
LIMIT
OF
THE
INDIRECT
ELISA
USING
ECH
MPA
AND
POLYCLONAL
(ECH
DSM
30179)
ANTISERA
71
3.6.3
THE
EFFECT
OF
POTATO
EXTRACT
ON
THE
DETECTION
LIMIT
OF
THE
INDIRECT
ELISA
TEST
73
3.6.4
DETECTION
OF
ECA
FROM
ARTIFICIALLY
INOCULATED
POTATO
TUBERS
USING
THE
INDIRECT
ELISA
73
3.6.5
THE
EFFECT
OF
LIQUID
ENRICHMENT
MEDIUM
(LEM)
ON
THE
DETECTION
LIMIT
OF
THE
INDIRECT
ELISA
76
3.6.6
THE
REACTION
OF
ECA
POLYCLONAL
ANTISERUM
WITH
OTHER
BACTERIAL
SPECIES
IN
A
DIRECT
ELISA
TEST
78
3.7
DETECTION
AND
IDENTIFICATION
OF
ECA
USING
IMMUNOFLUORESCENCE
(IF)
TEST
78
3.7.1
THE
DETECTION
LIMIT
OF
THE
IF
TEST.
82
3.8
DETECTION
AND
IDENTIFICATION
OF
ECA
USING
THE
POLYMERASE
CHAIN
REACTION
METHOD
(PCR)
82
3.8.1
THE
DETECTION
LIMIT
OF
THE
PCR
METHOD
USING
ECA
PRIMER
(Y1
&
Y2)
84
3.8.2
DETECTION
OF
ECA
FROM
ROTTED
POTATO
TUBER
TISSUE
USING
THE
PCR
METHOD
84
3.9
DETECTION
OF
SOFT
ROT
ERWINIAS
ON
/
IN
POTATO TUBERS
85
3.9.1
EVALUATION
OF
INCUBATION
METHODS
FOR
DETECTION
OF
SOFT
ROT
ERWINIAS
ON
/
IN
POTATO
TUBERS
(LATENT
CONTAMINATION)
86
VI
3.9.2
EFFECT
OF
TEMPERATURE
AND
INCUBATION
PERIOD
ON
THE
DETECTION
OF
ERWINIA
SPP.ON
I
IN
POTATO
TUBERS
INCUBATED
IN
AIR-TIGHT
CONTAINERS
87
3.9.3
COMPARISON
BETWEEN
INCUBATION
OF
POTATO
TUBERS
IN
AIR-TIGHT
JARS
(TUBERS
SUBMERGED
IN
STERILE
TAP
WATER
OR
IN
TRITON
X
100
SOLUTION)
AND
WET
PAPER
TOWEL
INCUBATION
METHODS
FOR
DETECTION
OF
ERWINIA
SPP.
ON
/
IN
POTATO
TUBERS
88
3.9.4
DETECTION
AND
IDENTIFICATION
OF
PECTOLYTIC
ERWINIA
SPECIES
ON
/
IN
TUBERS
OF
DIFFERENT
POTATO
VARIETIES
(LATENT
CONTAMINATION)
AND
ORIGIN
89
3.10
EFFECT
OF
TRITON
X
100
AND
TWEEN
80
(SURFACTANTS)
ON
THE
VIRULENCE
OF
ERWINIA
SPP.
(TUBER
INFECTIVITY
TEST)
90
3.11
SCREENING
POTATO
VARIETIES
FOR
TUBER
RESISTANCE
TO
INFECTION
BY
SOFT
TOT
ERWINIA
SPP.
95
3.11.1
THE
USE
OF
TRITON
X
100
(SURFACTANT)
IN
THE
SCREENING
OF
POTATO
VARIETIES
FOR
TUBER
RESISTANCE
TO
SOFT
ROT
DISEASE
CAUSED
BY
ERWINIA
SPP.
99
3.12
GREEN-HOUSE
EXPERIMENTS
101
3.12.1
EVALUATION
OF
THE
VIRULENCE
OF
DIFFERENT
ERWINIA
SPECIES
101
3.12.2
EFFECT
OF
TEMPERATURE
ON
THE
VIRULENCE
OF
ERWINIA
SPP.
(ECA
,
ECC
AND
ECH)
103
3.12.3
EFFECT
OF
INOCULUM
CONCENTRATION
ON
TUBER
SEED
PIECE
ROT,
EMERGENCE
AND
BLACKLEG
DISEASE
DEVELOPMENT
106
4
DISCUSSION
108
4.1
DEVELOPMENT
OF
SEMI
SELECTIVE
MEDIUM
FOR
ISOLATION
SOFT
ROT
ERWINIA
SPP.
108
4.2
DEVELOPMENT
OF
A
SEMI-SELECTIVE
MEDIUM
FOR
SELECTIVE
ISOLATION
OF
ERWINIA
CAROTOVORA
SUBSP.
CAROTOVORA
(ECC)
111
4.3
PRODUCTION
OF
MONOSPECIFIC
POLYCLONAL
ANTISERUM
AGAINST
ERWINIA
CAROTOVORA
SUBSP.
ATROSEPTICA
(ECA)
112
4.3.1
DEVELOPMENT
OF
A
METHOD
FOR
EXTRACTION
OF
ELECTROPHORESED
PROTEINS
FROM
SDS-PAGE
GELS
114
4.4
DETECTION
OF
ERWINIA
SPP.
USING
THE
INDIRECT
ELISA
METHOD
115
4.5
DETECTION
AND
IDENTIFICATION
OF
ECA
USING
THE
IMMUNOFLUORESCENCE
TEST
(IF
TEST)
117
VII
4.6
DETECTION
OF
ERWINIA
CAROTOVORA
SUBSP.
ATROSEPTICA
(ECA)
USING
POLYMERASE
CHAIN
REACTION
METHOD
(PCR)
118
4.7
EVALUATION
OF
INCUBATION
METHODS
FOR
DETECTION
OF
PECTOLYTIC
SOFT
ROT
ERWINIA
SPP.
ON
/
IN
POTATO
TUBERS
119
4.8
EFFECT
OF
INCUBATION
TEMPERATURE
AND
PERIOD
ON
THE
DETECTION
OF
ERWINIA
SPP.
ON
POTATO
TUBERS
INCUBATED
IN
AIR-TIGHT
JARS
120
4.9
COMPARISON
BETWEEN
INCUBATION
OF
TUBERS
SUBMERGED
IN
STERILE
TAP
WATER
OR
TRITON
X
100
SOLUTION
IN
AIR-TIGHT
JARS
WITH
THE
WET
PAPER
TOWEL
METHOD
OF
TUBER
INCUBATION
FOR
DETECTION
OF
ERWINIA
SPP.
122
4.10
DETECTION
AND
IDENTIFICATION
OF
PECTOLYTIC
ERWINIA
SPP.
ON
TUBERS
OF
DIFFERENT
POTATO
VARIETIES
AND
ORIGIN
122
4.11
EFFECT
OF
SURFACTANTS
(TRITON
X
100
AND
TWEEN
80)
ON
THE
VIRULENCE
OF
ERWINIA
SPP.
124
4.12
EVALUATION
OF
METHODS
FOR
SCREENING
POTATO
VARIETIES
FOR
TUBER
RESISTANCE
TO
SOFT
ROT
DISEASE
CAUSED
BY
ERWINIA
SPP.
125
4.13
EVALUATION
OF
THE
VIRULENCE
OF
ERWINIA
SPECIES
126
4.14
EFFECT
OF
TEMPERATURE
ON
THE
VIRULENCE
OF
ECA,
ECC
AND
ECH
STRAINS
UNDER
GROWTH
CONDITIONS
127
4.15
EFFECT
OF
INOCULUM
CONCENTRATION
ON
SEED
TUBER
ROT,
EMERGENCE
AND
BLACKLEG
DISEASE
DEVELOPMENT
127
5
GENERAL
CONCLUSIONS
130
6
SUMMARY
133
DEUTSCHE
ZUSAMMENFASSUNG
135A
7
LIST
OF
REFERENCES
136 |
| any_adam_object | 1 |
| author | Bdliya, Bulus Shapshi |
| author_facet | Bdliya, Bulus Shapshi |
| author_role | aut |
| author_sort | Bdliya, Bulus Shapshi |
| author_variant | b s b bs bsb |
| building | Verbundindex |
| bvnumber | BV010698821 |
| classification_tum | LAN 244d LAN 350d |
| ctrlnum | (OCoLC)613641783 (DE-599)BVBBV010698821 |
| discipline | Agrarwissenschaft Pflanzenbau |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV010698821 |
| illustrated | Illustrated |
| indexdate | 2024-08-18T00:14:25Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007140817 |
| oclc_num | 613641783 |
| open_access_boolean | |
| owner | DE-M49 DE-BY-TUM DE-12 DE-355 DE-BY-UBR |
| owner_facet | DE-M49 DE-BY-TUM DE-12 DE-355 DE-BY-UBR |
| physical | IX, 148 S. Ill., graph. Darst. |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| record_format | marc |
| spelling | Bdliya, Bulus Shapshi Verfasser aut Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers by Bulus Shapshi Bdliya 1995 IX, 148 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Göttingen, Univ., Diss., 1995 Knollennassfäule (DE-588)4385357-2 gnd rswk-swf Nachweis (DE-588)4115334-0 gnd rswk-swf Kartoffel (DE-588)4029804-8 gnd rswk-swf Erwinia (DE-588)4152948-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Knollennassfäule (DE-588)4385357-2 s Erwinia (DE-588)4152948-0 s Nachweis (DE-588)4115334-0 s DE-604 Kartoffel (DE-588)4029804-8 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007140817&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bdliya, Bulus Shapshi Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers Knollennassfäule (DE-588)4385357-2 gnd Nachweis (DE-588)4115334-0 gnd Kartoffel (DE-588)4029804-8 gnd Erwinia (DE-588)4152948-0 gnd |
| subject_GND | (DE-588)4385357-2 (DE-588)4115334-0 (DE-588)4029804-8 (DE-588)4152948-0 (DE-588)4113937-9 |
| title | Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers |
| title_auth | Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers |
| title_exact_search | Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers |
| title_full | Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers by Bulus Shapshi Bdliya |
| title_fullStr | Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers by Bulus Shapshi Bdliya |
| title_full_unstemmed | Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers by Bulus Shapshi Bdliya |
| title_short | Studies on the detection and identification of soft rot causing Erwinia species (Erwinia carotovora ssp. atroseptica (van Hall) Dye, Erwinia carotovora ssp. carotovora (Jones) Bergey et al., Erwinia chrysanthemi Burkholder et al.) on potato tubers |
| title_sort | studies on the detection and identification of soft rot causing erwinia species erwinia carotovora ssp atroseptica van hall dye erwinia carotovora ssp carotovora jones bergey et al erwinia chrysanthemi burkholder et al on potato tubers |
| topic | Knollennassfäule (DE-588)4385357-2 gnd Nachweis (DE-588)4115334-0 gnd Kartoffel (DE-588)4029804-8 gnd Erwinia (DE-588)4152948-0 gnd |
| topic_facet | Knollennassfäule Nachweis Kartoffel Erwinia Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007140817&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT bdliyabulusshapshi studiesonthedetectionandidentificationofsoftrotcausingerwiniaspecieserwiniacarotovorasspatrosepticavanhalldyeerwiniacarotovorasspcarotovorajonesbergeyetalerwiniachrysanthemiburkholderetalonpotatotubers |