Patch-clamp-Technik:
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
Heidelberg [u.a.]
Spektrum, Akad. Verl.
1996
|
| Schriftenreihe: | Labor im Fokus
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VII, 206 S. Ill., graph. Darst. |
| ISBN: | 3827400236 |
Internformat
MARC
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| 100 | 1 | |a Numberger, Markus |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Patch-clamp-Technik |c Markus Numberger ; Andreas Draguhn |
| 264 | 1 | |a Heidelberg [u.a.] |b Spektrum, Akad. Verl. |c 1996 | |
| 300 | |a VII, 206 S. |b Ill., graph. Darst. | ||
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| 650 | 7 | |a Patch Clamp Techniques |2 cabt | |
| 650 | 4 | |a Patch-Clamp Techniques | |
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Datensatz im Suchindex
| _version_ | 1806142860157779968 |
|---|---|
| adam_text |
MARKUS NUMBERGER/ANDREAS DRAGUHN PATCH-CLAMP - TECHNIK MIT EINEM
GELEITWORT VON BERT SAKMANN GELEITWORT IX VORWORT XI DANKSAGUNG XII I 1.
HISTORISCHE GRUNDLAGEN 1 .1 BIOELEKTRIZITAET 1 1 .2 DIE IONENTHEORIE 2 1
.3 DIE ENTWICKLUNG DER PATCH-CLAMP-TECHNIK 4 1.4 DIE WEITERENTWICKLUNG
DER TECHNIK 12 LITERATUR 15 2. MESSPLATZ UND PATCHPIPETTE 2.1 DIE OPTIK:
MIKROSKOP UND VIDEO 19 2.1.1 MIKROSKOP 19 2.1.2 VIDEOTECHNIK 22 2.2
MESSTISCH UND MECHANISCHE KOMPONENTEN 23 2.2.1 MESSTISCH UND KAEFIG 2 3
2.2.2 MIKROMANIPULATOR 28 2.2.3 PIPETTENHALTER 30 2.2.4 MESSKAMMERN 32
2.2.5 KAMMERPERFUSION UND APPLIKATIONSVERFAHREN 37 2.3 ELEKTRONISCHE
KOMPONENTEN 40 2.3.1 VORVERSTAERKER UND VERSTAERKER 40 2.3.2
STIMULATIONSGERAET 44 2.3.3 ZWISCHENVERSTAERKER UND FILTER 45 2.4
DATENVERARBEITUNG 46 2.4.1 OSZILLOSKOP 46 2.4.2 SCHREIBER 47 2.4.3
SPEICHERMEDIEN 47 2.4.4 AD/DA-WANDLER 49 2.4.5 COMPUTER 50 2.4.6
SOFTWARE 5 1 2.5 GLAESER, PIPETTEN UND ELEKTRODEN 53 2.5 .1 DIE
PATCHPIPETTE 53 2.5 .2 PIPETTENGLAESER 54 2.5.3 HERSTELLUNG DER PIPETTEN
57 2.5.4 PIPETTENLOESUNGEN 61 2.5.5 ELEKTRODEN 63 LITERATUR 65 3. DIE
PRAKTISCHE DURCHFUEHRUNG VON PATCH-CLAMP-EXPERIMENTEN 3 .1
FUNKTIONSPRINZIP EINES PATCH-CLAMP-VERSTAERKERS 69 3.2 MESSANORDNUNGEN UND
IHRE BESONDERHEITEN 73 3.2.1 BILDUNG DES SEAL 73 3.2.2 DIE
CELL-ATTACHED-KONFIGURATION 79 3.2.3 DIE INSIDE-OUT-KONFIGURATION 82
3.2.4 DIE WHOLE-CELL-KONFIGURATION (GANZZELLABLEITUNG) 84 3.2.5
CURRENT-CLAMP-MESSUNGEN 96 3 .2.6 DIE OUTSIDE-OUT-KONFIGURATION 9 9 3 .3
KORREKTUR VON OFFSET UND KAPAZITIVEN ARTEFAKTEN 10 1 3 .3.1 DAS
OFFSETPOTENTIAL 10 1 3.3.2 KAPAZITAETSKOMPENSATION 103 3.3.3 KOMPENSATION
DES SERIENWIDERSTANDES 106 3.3.4 NACHTRAEGLICHE SUBTRAKTIONSVERFAHREN 112
3.4 BRUMMEN, RAUSCHEN UND FILTERUNG 114 3.4.1 NETZBRUMMEN 114 3.4.2
RAUSCHEN 11 8 3.4.3 FILTERUNG 122 3 .5 AUSWERTUNG DER DATEN 126 3.5.1
EINZELKANALMESSUNGEN 126 3.5.2 GANZZELLMESSUNGEN 134 LITERATUR 140 4.
SPEZIELLE ANWENDUNGEN DER PATCH-CLAMP-TECHNIK 4.1 DISKONTINUIERLICHE
VERSTAERKER 14 3 4.2 INTERNE PIPETTENPERFUSION 14 5 4 .3 DER MAKROPATCH
146 4.4 DER PERFORATED PATCH 149 4.5 HETEROLOGE EXPRESSIONSSYSTEME 152
4.5.1 XENOPUS-OOCYTEN ALS HETEROLOGES EXPRESSIONSSYSTEM 15 3 4.5.2
TRANSFIZIERTE ZELLEN 156 4.6 PATCH-CLAMP-EXPERIMENTE AN
SCHNITTPRAEPARATEN 15 8 4.6 .1 DIE *BLINDE" METHODE 159 4.6.2 DIE
REINIGUNGSMETHODE 160 4.6.3 DIE MISCHTECHNIK 160 4.7
PATCH-CLAMP-EXPERIMENTE AN ORGANELLEN 162 4.7 .1 MITOCHONDRIEN UND
CHLOROPLASTEN 162 4.7.2 INTRAZELLULAERE MEMBRANEN 163 4.7.3 ZELLKERNE 163
4.8 ANFAERBEN VON ZELLEN 163 4.9 BILDGEBENDE VERFAHREN 165 4.10
KOMBINATION DER PATCH-CLAMP-TECHNIK MIT MOLEKULARBIOLOGISCHEN
NACHWEISMETHODEN 168 4.10.1 DIE TRANSKRIPTIONSMETHODE 169 4.10.2 DIE
RT-PCR-METHODE 17 1 4.11 KAPAZITAETSMESSUNGEN 17 3 4.12 ELEKTROCHEMISCHE
NACHWEISVERFAHREN 17 6 4.13 RAUSCHANALYSE 17 8 LITERATUR 18 1 ANHANG
ANHANG 1 : BAD- UND PIPETTENLOESUNGEN 189 ANHANG 2: HERSTELLER UND
HAENDLER 193 INDEX 203 |
| any_adam_object | 1 |
| author | Numberger, Markus Draguhn, Andreas 1961- |
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| ctrlnum | (OCoLC)263852518 (DE-599)BVBBV010682673 |
| discipline | Biologie Chemie |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-08-01T00:27:37Z |
| institution | BVB |
| isbn | 3827400236 |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-007130340 |
| oclc_num | 263852518 |
| open_access_boolean | |
| owner | DE-20 DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-19 DE-BY-UBM DE-355 DE-BY-UBR DE-703 DE-29 DE-11 DE-188 DE-578 DE-B768 |
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| physical | VII, 206 S. Ill., graph. Darst. |
| publishDate | 1996 |
| publishDateSearch | 1996 |
| publishDateSort | 1996 |
| publisher | Spektrum, Akad. Verl. |
| record_format | marc |
| series2 | Labor im Fokus |
| spelling | Numberger, Markus Verfasser aut Patch-clamp-Technik Markus Numberger ; Andreas Draguhn Heidelberg [u.a.] Spektrum, Akad. Verl. 1996 VII, 206 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Labor im Fokus Microelectrodes cabt Electrical Measuring Methods cabt Patch Clamp Techniques cabt Patch-Clamp Techniques Patch-Clamp-Methode (DE-588)4279564-3 gnd rswk-swf Patch-Clamp-Methode (DE-588)4279564-3 s DE-604 Draguhn, Andreas 1961- Verfasser (DE-588)121130282 aut GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007130340&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Numberger, Markus Draguhn, Andreas 1961- Patch-clamp-Technik Microelectrodes cabt Electrical Measuring Methods cabt Patch Clamp Techniques cabt Patch-Clamp Techniques Patch-Clamp-Methode (DE-588)4279564-3 gnd |
| subject_GND | (DE-588)4279564-3 |
| title | Patch-clamp-Technik |
| title_auth | Patch-clamp-Technik |
| title_exact_search | Patch-clamp-Technik |
| title_full | Patch-clamp-Technik Markus Numberger ; Andreas Draguhn |
| title_fullStr | Patch-clamp-Technik Markus Numberger ; Andreas Draguhn |
| title_full_unstemmed | Patch-clamp-Technik Markus Numberger ; Andreas Draguhn |
| title_short | Patch-clamp-Technik |
| title_sort | patch clamp technik |
| topic | Microelectrodes cabt Electrical Measuring Methods cabt Patch Clamp Techniques cabt Patch-Clamp Techniques Patch-Clamp-Methode (DE-588)4279564-3 gnd |
| topic_facet | Microelectrodes Electrical Measuring Methods Patch Clamp Techniques Patch-Clamp Techniques Patch-Clamp-Methode |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=007130340&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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