Studies on the desiccation resistance of various bacterial strains:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
1995
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Bonn, Univ., Diss., 1995 |
| Beschreibung: | V, 129 Bl. graph. Darst. |
Internformat
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Datensatz im Suchindex
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CONTENTS
1 DEVELOPMENT, PRODUCTION AND CERTIFICATION OF MICROBIOLOGICAL
REFERENCE MATERIALS
1.1 INTRODUCTION 2
1.2 DEVELOPMENT OF MICROBIOLOGICAL REFERENCE MATERIALS 2
1.3 REQUIREMENTS OF MICROBIOLOGICAL REFERENCE MATERIALS 4
1.3.1 REPRESENTATIVE 4
1.3.2 HOMOGENEITY 4
1.3.3 STABILITY 5
1.4 COLLABORATIVE STUDIES 7
1.5 CERTIFICATION 9
1.6 FIELDS OF APPLICATION 11
1.7 FURTHER RESEARCH 12
1.8 REFERENCES 13
2 RESISTANCE OF BACTERIAL STRAINS TO DRY CONDITIONS: USE OF
ANHYDROUS SILICA GEL IN A DESICCATION MODEL SYSTEM
2.1 INTRODUCTION 16
2.2 MATERIALS AND METHODS 19
2.2.1 BACTERIAL STRAINS 19
2.2.2 CULTURE MEDIA AND REAGENTS 22
2.2.3 CULTURE CONDITIONS 22
2.2.4 DRYING PROCEDURE 22
2.2.5 SAMPLE STORAGE 23
2.2.6 RECONSTITUTION AND SAMPLING 23
2.2.7 CALCULATIONS 24
2.3 RESULTS 28
2.4 DISCUSSION 36
2.4.1 GROUP 1: STABILIZATION OF THE CONCENTRATION OF CULTURABLE
ORGANISMS 36
2.4.2 GROUP 2: NO STABILIZATION OF THE CONCENTRATION OF CULTURABLE
ORGANISMS 38
2.4.3 GROUP 3: SHORT TERM SURVIVAL OF CULTURABLE ORGANISMS 39
2.4.4 GENERAL DISCUSSION 39
2.5 REFERENCES 43
I
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/945176775
3 CYTOPLASMIC ACCUMULATION OF COMPATIBLE SOLUTES AND THEIR
PROTECTIVE EFFECT AGAINST DESICCATION STRESS
3.1 INTRODUCTION 48
3.2 MATERIALS AND METHODS 52
3.2.1 DESICCATION ON ANHYDROUS SILICA GEL 52
3.2.1.1 BACTERIAL STRAINS 52
3.2.1.2 CULTURE MEDIA AND REAGENTS 52
3.2.1.3 CULTURE CONDITIONS 53
3.2.1.4 DRYING PROCEDURE 54
3.2.1.5 SAMPLE STORAGE 54
3.2.2 DETERMINATION OF THE CYTOPLASMIC ACCUMULATION OF
COMPATIBLE SOLUTES BY ISOCRATIC HPLC 55
3.2.2.1 BACTERIAL STRAINS 55
3.2.2.2 CULTURE MEDIA AND REAGENTS 55
3.2.2.3 INSTRUMENTS 55
3.2.2.4 PARAMETERS OF HPLC ANALYSIS 56
3.2.2.5 CULTIVATION, CELL HARVESTING, SUSPENDING IN DESICCATION MEDIUM,
WASHING 56
3.2.2.6 EXTRACTION 56
3
.2.21 CALCULATIONS 57
3.2.3 DETERMINATION OF THE CYTOPLASMIC ACCUMULATION OF
COMPATIBLE SOLUTES BY FMOC-HPLC 58
3.2.4 IDENTIFICATION OF ACCUMULATED COMPATIBLE SOLUTES BY
13
C-NMR SPECTROSCOPY 58
3.3 RESULTS 59
3.3.1
ESCHERICHIA COLIWR1 59
3.3.1.1 DESICCATION RESISTANCE 59
3.3.1.1.1 EFFECT OF THE CULTIVATION MEDIUM ON THE SURVIVAL AFTER
DESICCATION AND STORAGE AT 22 C 59
3.3.1.1.2 EFFECT OF THE DESICCATION MEDIUM ON THE SURVIVAL AFTER
DESICCATION AND STORAGE AT 22 C 61
3.3.1.2 ACCUMULATION OF COMPATIBLE SOLUTES 62
3.3.1.2.1 EFFECT OF THE CULTIVATION MEDIUM ON THE ACCUMULATION OF
COMPATIBLE SOLUTES 62
II
3.3.1.2.2 EFFECT OF THE DESICCATION MEDIUM ON THE ACCUMULATION OF
COMPATIBLE SOLUTES 64
3.3.2
PSEUDOMONAS AERUGINOSA ALM32 66
3.3.2.1 DESICCATION RESISTANCE 66
3.3.2.1.1 EFFECT OF THE CULTIVATION MEDIUM ON THE SURVIVAL AFTER
DESICCATION AND STORAGE AT 22 C 66
3.3.2.1.2 EFFECT OF THE DESICCATION MEDIUM ON THE SURVIVAL AFTER
DESICCATION AND STORAGE AT 22 C 66
3.3.2.2 ACCUMULATION OF COMPATIBLE SOLUTES 68
3.3.2.2.1 EFFECT OF THE CULTIVATION MEDIUM ON THE ACCUMULATION OF
COMPATIBLE SOLUTES 68
3.3.2.2.2 EFFECT OF THE DESICCATION MEDIUM ON THE ACCUMULATION OF
COMPATIBLE SOLUTES 68
3.3.3
AEROMONAS HYDROPHILA M800 69
3.3.3.1 DESICCATION RESISTANCE 69
3.3.3.1.1 EFFECT OF THE CULTIVATION MEDIUM ON THE SURVIVAL AFTER
DESICCATION AND STORAGE AT 22 C 69
3.3.3.1.2 EFFECT OF THE DESICCATION MEDIUM ON THE SURVIVAL AFTER
DESICCATION AND STORAGE AT 22 C 69
3.3.3.2 ACCUMULATION OF COMPATIBLE SOLUTES 70
3.3.3.2.1 EFFECT OF THE CULTIVATION MEDIUM ON THE ACCUMULATION OF
COMPATIBLE SOLUTES 70
3.3.3.2.2 EFFECT OF THE DESICCATION MEDIUM ON THE ACCUMULATION OF
COMPATIBLE SOLUTES 71
3.4 DISCUSSION 72
3.5 REFERENCES 78
III |
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| author | Janning, Birgit |
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| genre_facet | Hochschulschrift |
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| illustrated | Illustrated |
| indexdate | 2024-10-09T16:15:59Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006871530 |
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| physical | V, 129 Bl. graph. Darst. |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
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| spelling | Janning, Birgit Verfasser aut Studies on the desiccation resistance of various bacterial strains vorgelegt von Birgit Janning 1995 V, 129 Bl. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bonn, Univ., Diss., 1995 Lagerung (DE-588)4354980-9 gnd rswk-swf Bakterien (DE-588)4004296-0 gnd rswk-swf Resistenz (DE-588)4136408-9 gnd rswk-swf Biologisches Bezugsmaterial (DE-588)4211985-6 gnd rswk-swf Trocknung (DE-588)4124329-8 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Bakterien (DE-588)4004296-0 s Trocknung (DE-588)4124329-8 s Lagerung (DE-588)4354980-9 s Resistenz (DE-588)4136408-9 s Biologisches Bezugsmaterial (DE-588)4211985-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006871530&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Janning, Birgit Studies on the desiccation resistance of various bacterial strains Lagerung (DE-588)4354980-9 gnd Bakterien (DE-588)4004296-0 gnd Resistenz (DE-588)4136408-9 gnd Biologisches Bezugsmaterial (DE-588)4211985-6 gnd Trocknung (DE-588)4124329-8 gnd |
| subject_GND | (DE-588)4354980-9 (DE-588)4004296-0 (DE-588)4136408-9 (DE-588)4211985-6 (DE-588)4124329-8 (DE-588)4113937-9 |
| title | Studies on the desiccation resistance of various bacterial strains |
| title_auth | Studies on the desiccation resistance of various bacterial strains |
| title_exact_search | Studies on the desiccation resistance of various bacterial strains |
| title_full | Studies on the desiccation resistance of various bacterial strains vorgelegt von Birgit Janning |
| title_fullStr | Studies on the desiccation resistance of various bacterial strains vorgelegt von Birgit Janning |
| title_full_unstemmed | Studies on the desiccation resistance of various bacterial strains vorgelegt von Birgit Janning |
| title_short | Studies on the desiccation resistance of various bacterial strains |
| title_sort | studies on the desiccation resistance of various bacterial strains |
| topic | Lagerung (DE-588)4354980-9 gnd Bakterien (DE-588)4004296-0 gnd Resistenz (DE-588)4136408-9 gnd Biologisches Bezugsmaterial (DE-588)4211985-6 gnd Trocknung (DE-588)4124329-8 gnd |
| topic_facet | Lagerung Bakterien Resistenz Biologisches Bezugsmaterial Trocknung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006871530&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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