Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
1995
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Bonn, Univ., Diss., 1995 |
| Beschreibung: | XII, 131 S. Ill., graph. Darst. |
Internformat
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| 300 | |a XII, 131 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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| adam_text |
CONTENT
I.
I.
III.
IV.
ACKNOWLEDGEMENTS.
LIST OF ABBREVIATIONS
LIST OF FIGURES.
LIST OF TABLES.
.IV
.VI
.IX
XII
.1
1. INTRODUCTION.
3
1.1
FUNCTION OF COMPLEX CARBOHYDRATES M NATURE.
1.2
SYNTHESIS OF COMPLEX CARBOHYDRATES BY CHEMICAL AND ENZYMATIC
.
1
METHODS
.
1.3
GLYCOSYLTRANSFERASES.^
2.
RESULTS.
2.1
HUMAN $-L,
4
-GALACTOSYLTRANSFERASE.
2.1.1 LARGE-SCALE HETEROLOGOUS EXPRESSION OF 0-1,4-GALACTOSYL-
TRANSFERASE.
2.1.2 SYNTHESIS OF N-ACETYLLACTOSAMINE BY EMPLOYING TRANSFORMED
WHOLE YEAST CELLS.
2.1.3 LARGE-SCALE HETEROLOGOUS EXPRESSION OF HIS-TAG-0-1,4-
.
2.2
HUMAN .
2.2.1 LARGE-SCALE HETEROLOGOUS EXPRESSION OF A
2
,
6
-SIALYLTRANSFERASE
2.2.2 COMBINED USE OF 0-GALACTOSIDASE AND A-2,6-SIALYLTRANSFERASE
2.3
FOR A ONE-POT SYNTHESIS OF SIALYL OLIGOSACCHARIDES.
MURINE A-L,
3
-GALACTOSYLTRANSFERASE.
2.3.1 ATTEMPTED EXPRESSION OF A-1,
3
-GALACTOSYLTRANSFERASE IN E. COLI
1
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/943884551
2.4
A-L,2-MANNOSYLTRANSFERASE FROM YEAST.50
2.4.1 LARGE-SCALE HETEROLOGOUS EXPRESSION OF
A-L,2-MANNOSYLTRANSFERASE.52
2.4.2 PURIFICATION OF A-L,2-MANNOSYLTRANSFERASE.61
2.4.3 ACCEPTOR SPECIFICITY OF A-L,2-MANNOSYLTRANSFERASE.65
2.4.4 TRANSFORMED WHOLE CELLS EXPRESSING A-1,2-MANNOSY 1 TRANSFERASE
AS CATALYSTS FOR THE ENZYMATIC SYNTHESIS OF OLIGOSACCHARIDES AND
GLYCOPEPTIDES.73
3. SUMMARY AND OUTLOOK.76
4. EXPERIMENTAL PROCEDURES
.80
4.1
HUMAN $-L,4-GALACTOSYHRANSFERASE.80
4.1.1 LARGE-SCALE HETEROLOGOUS EXPRESSION OF 0-1,4-GALACTOSYL-
TRANSFERASE.80
4.1.2 SYNTHESIS OF TF-ACETYLLACTOSAMINE BY EMPLOYING TRANSFORMED
WHOLE YEAST CELLS.87
4.1.3 LARGE-SCALE HETEROLOGOUS EXPRESSION OF HIS-TAG-0-1,4-
GALACTOSYLTRANSFERASE.89
4.2 HUMAN A-2,6-SIALYLTRANSFERASE.90
4.2.1 LARGE-SCALE HETEROLOGOUS EXPRESSION OF A-2,6-SIALYLTRANSFERASE.90
4.2.2 COMBINED USE OF 0-GALACTOSIDASE AND A-2,6-SIALYLTRANSFERASE
FOR A ONE-POT SYNTHESIS OF SIALYL OLIGOSACCHARIDES.91
4.3
MURINE A-LJ-GALACTOSYLTRANSFERASE.93
4.3.1 ATTEMPTED EXPRESSION OF A-L,3-GALACTOSYLTRANSFERASE IN
E. COLI.93
4.4 A
-1,2-MANNOSYLTRANSFERASE FROM YEAST.94
4.4.1 LARGE-SCALE HETEROLOGOUS EXPRESSION OF
A-L,2-MANNOSYLTRANSFERASE.94
II
.98
4.4.2 PURIFICATION OF A-L,
2
-MANNOSYLTRANSFERASE.
4.4.3 ACCEPTOR SPECIFICITY OF A-L,
2
-MANNOSYLTRANSFERASE.
4.4.4 TRANSFORMED WHOLE CELLS EXPRESSING A-L,
2
-MANNOSYLTRANSFERASE
AS CATALYSTS FOR THE ENZYMATIC SYNTHESIS OF OLIGOSACCHARIDES AND
GLYCOPEPTIDES.
.104
5. REFERENCES.
.129
6. LIST OF PUBLICATIONS.
.131
7. CURRICULUM VITAE.*.
ILL |
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| id | DE-604.BV010173992 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T16:16:39Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006757479 |
| oclc_num | 54860578 |
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| owner_facet | DE-91 DE-BY-TUM DE-29T DE-355 DE-BY-UBR DE-83 DE-11 |
| physical | XII, 131 S. Ill., graph. Darst. |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| record_format | marc |
| spelling | Herrmann, Guido F. Verfasser aut Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli Guido F. Herrmann 1995 XII, 131 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Bonn, Univ., Diss., 1995 Glycosyltransferasen (DE-588)4157751-6 gnd rswk-swf Escherichia coli (DE-588)4070959-0 gnd rswk-swf Heterologe Genexpression (DE-588)4342015-1 gnd rswk-swf Saccharomyces cerevisiae (DE-588)4178812-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Saccharomyces cerevisiae (DE-588)4178812-6 s Glycosyltransferasen (DE-588)4157751-6 s Heterologe Genexpression (DE-588)4342015-1 s DE-604 Escherichia coli (DE-588)4070959-0 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006757479&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Herrmann, Guido F. Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli Glycosyltransferasen (DE-588)4157751-6 gnd Escherichia coli (DE-588)4070959-0 gnd Heterologe Genexpression (DE-588)4342015-1 gnd Saccharomyces cerevisiae (DE-588)4178812-6 gnd |
| subject_GND | (DE-588)4157751-6 (DE-588)4070959-0 (DE-588)4342015-1 (DE-588)4178812-6 (DE-588)4113937-9 |
| title | Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli |
| title_auth | Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli |
| title_exact_search | Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli |
| title_full | Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli Guido F. Herrmann |
| title_fullStr | Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli Guido F. Herrmann |
| title_full_unstemmed | Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli Guido F. Herrmann |
| title_short | Heterologous expression of glycosyltransferases in S. cerevisiae and E. coli |
| title_sort | heterologous expression of glycosyltransferases in s cerevisiae and e coli |
| topic | Glycosyltransferasen (DE-588)4157751-6 gnd Escherichia coli (DE-588)4070959-0 gnd Heterologe Genexpression (DE-588)4342015-1 gnd Saccharomyces cerevisiae (DE-588)4178812-6 gnd |
| topic_facet | Glycosyltransferasen Escherichia coli Heterologe Genexpression Saccharomyces cerevisiae Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006757479&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT herrmannguidof heterologousexpressionofglycosyltransferasesinscerevisiaeandecoli |