Colloidal gold post-embedding immunocytochemistry: with 8 tables
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Stuttgart [u.a.]
Fischer
1995
|
| Schriftenreihe: | Progress in histochemistry and cytochemistry
29,4 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VII, 163 S. zahlr. Ill., graph. Darst. |
| ISBN: | 3437116436 1560814217 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV010124774 | ||
| 003 | DE-604 | ||
| 005 | 20150427 | ||
| 007 | t | ||
| 008 | 950327s1995 gw ad|| |||| 00||| eng d | ||
| 016 | 7 | |a 943825911 |2 DE-101 | |
| 020 | |a 3437116436 |9 3-437-11643-6 | ||
| 020 | |a 1560814217 |9 1-56081-421-7 | ||
| 035 | |a (OCoLC)33286075 | ||
| 035 | |a (DE-599)BVBBV010124774 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 |a DE-19 |a DE-12 |a DE-188 |a DE-578 | ||
| 050 | 0 | |a QR187.I45 | |
| 084 | |a YV 4511 |0 (DE-625)154224:12917 |2 rvk | ||
| 100 | 1 | |a Bendayan, Moi͏̈se |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Colloidal gold post-embedding immunocytochemistry |b with 8 tables |c Moïse Bendayan |
| 264 | 1 | |a Stuttgart [u.a.] |b Fischer |c 1995 | |
| 300 | |a VII, 163 S. |b zahlr. Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Progress in histochemistry and cytochemistry |v 29,4 | |
| 650 | 7 | |a Immunocytochemistry |2 cabt | |
| 650 | 7 | |a Immunocytochemie |2 gtt | |
| 650 | 7 | |a Kolloïdaal goud |2 gtt | |
| 650 | 4 | |a Colloidal gold | |
| 650 | 4 | |a Immunocytochemistry | |
| 650 | 4 | |a Immunocytochemistry |x Technique | |
| 650 | 0 | 7 | |a Immuncytochemie |0 (DE-588)4161368-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Goldmarkierung |0 (DE-588)4245618-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Einbettung |g Mikroskopie |0 (DE-588)4321430-7 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Einbettung |g Mikroskopie |0 (DE-588)4321430-7 |D s |
| 689 | 0 | 1 | |a Immuncytochemie |0 (DE-588)4161368-5 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Immuncytochemie |0 (DE-588)4161368-5 |D s |
| 689 | 1 | 1 | |a Goldmarkierung |0 (DE-588)4245618-6 |D s |
| 689 | 1 | |5 DE-604 | |
| 830 | 0 | |a Progress in histochemistry and cytochemistry |v 29,4 |w (DE-604)BV000005277 |9 29,4 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006724159&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-006724159 | ||
Datensatz im Suchindex
| _version_ | 1804124519121551360 |
|---|---|
| adam_text | Contents
1 Introduction 1
2 Immunoglobulins 4
3 Protein A, protein G, protein A/G 9
4 Colloidal gold marker 11
4.1 Preparation of the colloidal gold 14
5 Preparation of the protein gold complexes 17
5.1 Protein A gold complex 17
5.2 Protein G gold and protein A/G gold complexes 21
5.3 Immunoglobulin gold complexes 21
5.4 Preparation of polyclonal antibody gold complexes 23
5.5 Preparation of monoclonal antibody gold complexes 23
5.6 Streptavidin gold complex 23
5.7 Protein gold dextran complexes 24
5.8 Further characteristics of the protein gold complexes 25
6 Preparation of the tissues 25
6.1 Light microscopy 26
6.2 Electron microscopy 26
6.2.1 Chemical fixation 26
6.2.2 Physical fixation 27
6.2.2.1 Microwave irradiation 27
6.2.2.2 Cryofixation, cryosubstitution, molecular distillation 28
6.3 Vehicles for fixatives 30
6.4 Methods of fixation 30
6.5 Dehydration 31
6.6 Embedding of tissue samples 31
Embedding in epoxy resins 32
Embedding in methacrylate resins 32
Embedding in Lowicryl , 33
Embedding in Unicryl .... . . . 34
Embedding in LR White .•_••• 34
Embedding in LR Gotd . T. .. . 34
Embedding in crosslinked albumin 34
6.7 Resinless sections 35
6 8 Frozen sections 35
6.9 Tissue sectioning 36
7 Labeling protocol 36
7.1 Light microscopy 37
7.2 Electron microscopy 39
VI • Contents
8 Immunogold results 44
8.1 Light microscopy 44
8.2 Visualization of immunogold reaction on semithin sections by backscat
tered electron imaging 44
8.3 Electron microscopy 45
8.4 Additional recommendations and problems of labeling 51
8.5 Background staining 59
9 Strategy for optimal immunolabeling 64
10 Controls of specificity 65
11 Limitations of the post embedding technique 72
11.1 Choice of the antibody: Polyclonal versus monoclonal 79
11.2 Choice of the probe 81
12 Resolution 83
12.1 Reliability of the approach 84
Specificity 84
Sensitivity 84
Efficiency 84
Accuracy 84
Precision 85
Practicality 85
13 Double labeling 85
13.1 Light microscopy 85
13.2 Electron microscopy 86
13.2.1 Gold labeled antibodies: Direct technique 86
13.2.2 Gold labeled antibodies: Indirect technique 86
13.2.3 Multiple labeling on the same face of the tissue section with a fixation step 86
13.2.4 Protein A gold multiple labeling: Two antigens on the same face of the
tissue section 88
13.2.5 Protein A gold multiple labeling: Two antigens on the two faces of the
same tissue section 88
13.2.6 Multiple labeling using gold labeled antigens 89
13.3 Combination of enzyme cytochemistry and immunocytochemistry ... 91
13.4 Combination of autoradiography and immunocytochemistry 93
14 Amplification ... 96
15 Quantitative evaluations 98
15.1 Evaluation of the density of labeling 100
15.2 Correlation between quantitative immunolabelings and known concen¬
trations of antigens 102
15.3 Evaluation of the site of labeling 107
16 Dynamics of molecules in tissues and cells as revealed by immunocyto¬
chemistry ... 109
Contents • VII
16.1 Evaluation of the passage of serum proteins through the renal glomerular
wall 115
16.2 Diffusion of serum proteins through the aortic wall 116
16.3 Transcytosis through capillary endothelial cells 116
16.4 Endocytotic activity by epithelial cells 119
17 Pathology 122
18 Safety precautions 127
19 Conclusion 129
References 133
Subject index 161
|
| any_adam_object | 1 |
| author | Bendayan, Moi͏̈se |
| author_facet | Bendayan, Moi͏̈se |
| author_role | aut |
| author_sort | Bendayan, Moi͏̈se |
| author_variant | m b mb |
| building | Verbundindex |
| bvnumber | BV010124774 |
| callnumber-first | Q - Science |
| callnumber-label | QR187 |
| callnumber-raw | QR187.I45 |
| callnumber-search | QR187.I45 |
| callnumber-sort | QR 3187 I45 |
| callnumber-subject | QR - Microbiology |
| classification_rvk | YV 4511 |
| ctrlnum | (OCoLC)33286075 (DE-599)BVBBV010124774 |
| discipline | Medizin |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02072nam a2200529 cb4500</leader><controlfield tag="001">BV010124774</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20150427 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">950327s1995 gw ad|| |||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">943825911</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">3437116436</subfield><subfield code="9">3-437-11643-6</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">1560814217</subfield><subfield code="9">1-56081-421-7</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)33286075</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV010124774</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-188</subfield><subfield code="a">DE-578</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QR187.I45</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">YV 4511</subfield><subfield code="0">(DE-625)154224:12917</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Bendayan, Moi͏̈se</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Colloidal gold post-embedding immunocytochemistry</subfield><subfield code="b">with 8 tables</subfield><subfield code="c">Moïse Bendayan</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Stuttgart [u.a.]</subfield><subfield code="b">Fischer</subfield><subfield code="c">1995</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">VII, 163 S.</subfield><subfield code="b">zahlr. Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Progress in histochemistry and cytochemistry</subfield><subfield code="v">29,4</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Immunocytochemistry</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Immunocytochemie</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Kolloïdaal goud</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Colloidal gold</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunocytochemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Immunocytochemistry</subfield><subfield code="x">Technique</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Immuncytochemie</subfield><subfield code="0">(DE-588)4161368-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Goldmarkierung</subfield><subfield code="0">(DE-588)4245618-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Einbettung</subfield><subfield code="g">Mikroskopie</subfield><subfield code="0">(DE-588)4321430-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Einbettung</subfield><subfield code="g">Mikroskopie</subfield><subfield code="0">(DE-588)4321430-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Immuncytochemie</subfield><subfield code="0">(DE-588)4161368-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Immuncytochemie</subfield><subfield code="0">(DE-588)4161368-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Goldmarkierung</subfield><subfield code="0">(DE-588)4245618-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Progress in histochemistry and cytochemistry</subfield><subfield code="v">29,4</subfield><subfield code="w">(DE-604)BV000005277</subfield><subfield code="9">29,4</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006724159&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-006724159</subfield></datafield></record></collection> |
| id | DE-604.BV010124774 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T17:46:57Z |
| institution | BVB |
| isbn | 3437116436 1560814217 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006724159 |
| oclc_num | 33286075 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-12 DE-188 DE-578 |
| owner_facet | DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-12 DE-188 DE-578 |
| physical | VII, 163 S. zahlr. Ill., graph. Darst. |
| publishDate | 1995 |
| publishDateSearch | 1995 |
| publishDateSort | 1995 |
| publisher | Fischer |
| record_format | marc |
| series | Progress in histochemistry and cytochemistry |
| series2 | Progress in histochemistry and cytochemistry |
| spelling | Bendayan, Moi͏̈se Verfasser aut Colloidal gold post-embedding immunocytochemistry with 8 tables Moïse Bendayan Stuttgart [u.a.] Fischer 1995 VII, 163 S. zahlr. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Progress in histochemistry and cytochemistry 29,4 Immunocytochemistry cabt Immunocytochemie gtt Kolloïdaal goud gtt Colloidal gold Immunocytochemistry Immunocytochemistry Technique Immuncytochemie (DE-588)4161368-5 gnd rswk-swf Goldmarkierung (DE-588)4245618-6 gnd rswk-swf Einbettung Mikroskopie (DE-588)4321430-7 gnd rswk-swf Einbettung Mikroskopie (DE-588)4321430-7 s Immuncytochemie (DE-588)4161368-5 s DE-604 Goldmarkierung (DE-588)4245618-6 s Progress in histochemistry and cytochemistry 29,4 (DE-604)BV000005277 29,4 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006724159&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bendayan, Moi͏̈se Colloidal gold post-embedding immunocytochemistry with 8 tables Progress in histochemistry and cytochemistry Immunocytochemistry cabt Immunocytochemie gtt Kolloïdaal goud gtt Colloidal gold Immunocytochemistry Immunocytochemistry Technique Immuncytochemie (DE-588)4161368-5 gnd Goldmarkierung (DE-588)4245618-6 gnd Einbettung Mikroskopie (DE-588)4321430-7 gnd |
| subject_GND | (DE-588)4161368-5 (DE-588)4245618-6 (DE-588)4321430-7 |
| title | Colloidal gold post-embedding immunocytochemistry with 8 tables |
| title_auth | Colloidal gold post-embedding immunocytochemistry with 8 tables |
| title_exact_search | Colloidal gold post-embedding immunocytochemistry with 8 tables |
| title_full | Colloidal gold post-embedding immunocytochemistry with 8 tables Moïse Bendayan |
| title_fullStr | Colloidal gold post-embedding immunocytochemistry with 8 tables Moïse Bendayan |
| title_full_unstemmed | Colloidal gold post-embedding immunocytochemistry with 8 tables Moïse Bendayan |
| title_short | Colloidal gold post-embedding immunocytochemistry |
| title_sort | colloidal gold post embedding immunocytochemistry with 8 tables |
| title_sub | with 8 tables |
| topic | Immunocytochemistry cabt Immunocytochemie gtt Kolloïdaal goud gtt Colloidal gold Immunocytochemistry Immunocytochemistry Technique Immuncytochemie (DE-588)4161368-5 gnd Goldmarkierung (DE-588)4245618-6 gnd Einbettung Mikroskopie (DE-588)4321430-7 gnd |
| topic_facet | Immunocytochemistry Immunocytochemie Kolloïdaal goud Colloidal gold Immunocytochemistry Technique Immuncytochemie Goldmarkierung Einbettung Mikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006724159&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000005277 |
| work_keys_str_mv | AT bendayanmoise colloidalgoldpostembeddingimmunocytochemistrywith8tables |