Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
1994
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Köln, Univ., Diss., 1994 |
| Beschreibung: | 101 S. Ill., graph. Darst. |
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| 100 | 1 | |a Liu, Jun-qi |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato |c vorgelegt von Jun-qi Liu |
| 264 | 1 | |c 1994 | |
| 300 | |a 101 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
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| adam_text |
CONTENTS
1.
INTRODUCTION
-
-
-
-
-
_
6
1.1.
OVERVIEW
OF
MALE
GAMETOPHYTE
(POLLEN)
DEVELOPMENT
.
6
1.2.
GENE
EXPRESSION
DURING
MALE
GAMETOPHYTE
DEVELOPMENT
.
8
1.3.
MECHANISMS
OF
POLLEN
GERMINATION
AND
POLLEN
TUBE
GROWTH
.
9
1.4.
GAMETOPHYTIC
GENE
EXPRESSION
AND
POLLEN-PISTIL
INTERACTION:
GAMETOPHYTIC
SELF-INCOMPATIBILITY
.
11
1.5.
PROTEIN
KINASE-MEDIATED
SIGNAL
TRANSDUCTION
IN
PLANTS
AND
THE
POSSIBLE
ROLE
OF
PROTEIN
KINASES
IN
POLLEN-PISTIL
INTERACTION
.
13
1.6.
INACTIVATION
OF
GENE
EXPRESSION
BY
ANTISENSE
INHIBITION
.
16
1.7.
OUTLINE
OF
DIE
RESEARCH
REPORTED
IN
THIS
THESIS
.
17
2.
MATERIALS
AND
METHODS
-------------------------------------------------------
19
2.1.
MATERIALS
.
19
2.1.1.
PLANT
MATERIALS
.
19
2.1.2.
BACTERIAL
STRAINS
AND
VECTORS
.
19
2.1.3.
ENZYMES,
CHEMICALS,
ROUTINELY
USED
MEDIA
AND
BUFFERS
.
20
2.1.4.
ABBREVIATIONS
.
21
2.2.
METHODS
.
22
2.2.1.
IN
VITRO
POLLEN
GERMINATION
.
22
2.2.2.
ISOLATION
OF
PLANT
GENOMIC
DNA
.
22
2.2.3.
ISOLATION
OF
TOTAL
RNA
AND
POLY(A)
+
RNA
.
23
2.2.4.
CDNA
LIBRARY
CONSTRUCTION
.
23
2.2.5.
SUBTRACTIVE
HYBRIDIZATION
.
24
2.2.5.1.
PHOTOBIOTINYLATION
OF
SUBTRACTOR
MRNA
.
24
2.2.5.2.
RADIOACTIVE
LABELLING
OF
CDNAS
FOR
SUBTRACTION
.
24
2.2.5.3.
SUBTRACTIVE
SOLUTION
HYBRIDIZATION
.
25
2.2.6.
CONSTRUCTION
OF
A
PARTIAL
GENOMIC
LIBRARY
.
25
2.2.7.
PREPARATION
OF
PLASMID
DNA
.
25
2.2.8.
POLYMERASE
CHAIN
REACTIONS
(PCR)
.
26
2.2.9.
SYNTHESIS
AND
PURIFICATION
OF
OLIGONUCLEOTIDES
.
26
2.2.10.
NUCLEIC
ACID
HYBRIDIZATION
.
26
2.2.10.1.
PREPARATION
OF
RADIOACTIVE
PROBES
.
27
2.2.10.2.
PLAQUE
FILTER
HYBRIDIZATION
.
27
2.2.10.3.
RNA
GEL
BLOTTING
ANALYSIS
.
27
2.2.10.4.
GENOMIC
SOUTHERN
BLOTTING
ANALYSIS
.
28
2.2.11.
SUBCLONING
OF
DNA
FRAGMENTS
AND
NUCLEOTIDE
SEQUENCING
ANALYSIS
.
28
2.2.12.
OVER-EXPRESSION
AND
PURIFICATION
OF
PROTEINS
FROM
E.
COLI
.
28
2.2.13.
IMMUNIZATION
OF
RABBITS
AND
THE
RECOVERY
OF
ANTISERUM
.
29
2.2.14.
STABLE
PLANT
TRANSFORMATION
.
29
2.2.14.1.
TRANSFORMATION
OF
AGROBACTERIUM
TUMEFACIENS
.
29
2.2.14.2.
A.
TUMEFACIENS-MEDIATED
TRANSFORMATION
OF
POTATO
.
30
2.2.15.
EXTRACTION
AND
QUANTITATION
OF
TOTAL
PROTEIN
FROM
PLANT
TISSUES
.
31
2.2.16.
SDS-PAGE
AND
WESTERN
BLOTTING
ANALYSIS
.
31
2.2.17.
COMPUTER
DATA
PROCESSING
.
32
3.
RESULTS
---------------------------------------------------------------------------------------
33
3.1.
ANALYSIS
OF
THE
GAMETOPHYTIC
EXPRESSION
OFTHES-LOCUS
.
33
3.1.1.
DETECTION
OF
SLG
MESSENGER
RNA
SEQUENCES
BY
RT-PCR
.
33
3.1.2.
DIFFERENCE
IN
MRNA
POOL
BETWEEN
POLLEN
CARRYING
DIFFERENT
ALLELES
.
35
3.2.
ISOLATION
AND
NUCLEOTIDE
SEQUENCING
ANALYSIS
OF
THE
POLLEN-SPECIFIC
CDNA
SB401
.
36
3.2.1.
NUCLEOTIDE
SEQUENCING
AND
AMINO
ACID
SEQUENCE
ALIGNMENT
OF
SB401
.
36
3.2.2.
TISSUE-SPECIFIC
AND
DEVELOPMENTAL
ACCUMULATION
OF
SB401
MRNA
.
39
3.2.3.
DETECTION
OF
ANOTHER
TRANSCRIPT
SPECIES
WITH
LOW
HOMOLOGY
TO
SB401
.
41
3.2.4.
GENOMIC
SOUTHERN
BLOT
ANALYSIS
OF
SB401
GENE
.
41
3.2.5.
SYNTHESIS
AND
PURIFICATION
OF
SB401
PROTEIN
IN
E.
COLI
.
42
3.2.6.
DETECTION
OF
THE
NATIVE
SB401
PROTEIN
IN
MATURE
AND
GERMINATED
POLLEN
.
44
3.3.
CHARACTERIZATION
OF
A
POLLEN-EXPRESSED
PROTEIN
KINASE
GENE
.
46
3.3.1.
ISOLATION
OF
A
PROTEIN
KINASE
CDNA
CLONE
FROM
THE
CDNA
LIBRARY
OF
IN
VITRO
GERMINATED
POLLEN
.
46
3.3.2.
AMINO
ACID
SEQUENCE
ALIGNMENT
OF
SBPK
AND
THE
RELATED
PROTEIN
KINASES
.
48
3.3.3.
SPATIAL
AND
TEMPORAL
EXPRESSION
OF
THE
PROTEIN
KINASE
(SBPK)
GENE
.
50
3.3.4.
GENOMIC
STRUCTURE
OF
THE
SBPK
GENE
.
52
3.3.5.
ISOLATION
AND
NUCLEOTIDE
SEQUENCING
OF
A
SBPK
GENOMIC
CLONE
.
53
3.4.
'LOSS-OF-FIMCTION'
STUDIES:
ANTISENSE
INHIBITION
OF
SBPK
GENE
IN
TRANSGENIC
PLANTS
.
56
3.4.1.
CONSTRUCTION
OF
CHIMERIC
'ANTI-SENSE'
GENES
.
56
3.4.2.
INTEGRATION
OF
THE
'ANTI-SENSE'
GENE
IN
THE
GENOME
OF
A
DIPLOID
POTATO
LINE
.
59
3.4.3.
EXPRESSION
OF
THE
INTEGRATED
ANTISENSE
SBPK
GENES
IN
TRANSGENIC
PLANTS
.
61
3.4.4.
INACTIVATION
OF
SBPK
GENE
AND
THE
INFLUENCE
ON
POLLEN
FORMATION
.
63
4.
DISCUSSION
-------------------------------------------------------------------------------
68
4.1.
THE
STYLE-EXPRESSED
SLG
GENE
IS
NOT
EXPRESSED
IN
GAMETOPHYTIC
PATHWAY
IN
SOLATIUM
BERTHAULTII
.
68
4.2.
NO
QUALITATIVE
DIFFERENCE
IN
MRNA
POOL
WAS
FOUND
BETWEEN
TWO
POLLEN
GENOTYPES
AFTER
IN
VITRO
GERMINATION
AND
POLLEN
TUBE
GROWTH
.
71
4.3.
CHARACTERIZATION
OF
SB401
CDNA
AND
THE
SUGGESTED
ROLE
OF
SB401
PROTEIN
IN
POLLEN
FORMATION
.
72
4.3.1.
SB401
CDNA
ENCODES
A
NOVEL
PROTEIN
SPECIFICALLY
PRESENT
IN
GERMINATED
POLLEN
AS
WELL
AS
IN
MATURE
POLLEN
.
72
4.3.2.
SPECIAL
STRUCTURAL
FEATURES
AND
THE
POSSIBLE
ROLE
OF
SB401
PROTEIN
IN
POLLEN
MATURATION
.
72
4.4.
CHARACTERISTICS
OF
THE
POLLEN-EXPRESSED
PROTEIN
KINASE
SBPK
.
74
4.4.1.
EXPRESSION
PATTERN
OF
THE
SBPK
GENE
IN
POTATO
SOLANUM
BERTHAULTII
.
74
4.4.2.
SBPK
GENE
ENCODES
THE
CATALYTIC
SUBUNIT
OF
A
PUTATIVE
CYCLIC-NUCLEOTIDE
DEPENDENT
PROTEIN
KINASE
.
75
4.5.
EXPRESSION
OF
THE
CHIMERIC
ANTISENSE
SBPK
GENE
CAUSED
SIGNIFICANT
REDUCTION
OF
SBPK
MRNA
LEVEL
IN
MATURE
ANTHER
.
76
4.6.
INTEGRATION
OF
THREE
COPIES
OF
ANTISENSE
GENE
LEADS
TO
CO-SUPPRESSION
OF
BOTH
THE
INTEGRATED
ANTISENSE
GENES
AND
THE
ENDOGENOUS
SBPK
GENE
.
77
4.7.
THE
EFFECT
OF
SILENCING
SBPK
GENE
EXPRESSION
BY
CO-SUPPRESSION
ON
POLLEN
DEVELOPMENT
.
79
5.
SUMMARY
------------------------------------------------------------------------------------
81
6.
ZUSAMMENFASSUNG
--------------------------------------------------------------------
83
7.
REFERENCES
-------------------------------------------------------------------------------
85
ACKNOWLEDGEMENT
CURRICULUM
VITAE |
| any_adam_object | 1 |
| author | Liu, Jun-qi |
| author_facet | Liu, Jun-qi |
| author_role | aut |
| author_sort | Liu, Jun-qi |
| author_variant | j q l jql |
| building | Verbundindex |
| bvnumber | BV010081770 |
| classification_tum | BIO 180d BIO 608d BIO 456d BIO 470d BIO 450d BIO 220d BIO 453d LAN 220d CHE 860d LAN 350d |
| ctrlnum | (OCoLC)258087533 (DE-599)BVBBV010081770 |
| discipline | Biologie Agrarwissenschaft Chemie Pflanzenbau |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV010081770 |
| illustrated | Illustrated |
| indexdate | 2024-08-19T00:35:36Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006691335 |
| oclc_num | 258087533 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-M49 DE-BY-TUM DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-M49 DE-BY-TUM DE-11 |
| physical | 101 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| record_format | marc |
| spelling | Liu, Jun-qi Verfasser aut Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato vorgelegt von Jun-qi Liu 1994 101 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1994 Inhibition (DE-588)4273127-6 gnd rswk-swf Wachstum (DE-588)4064115-6 gnd rswk-swf Pollenschlauch (DE-588)4277435-4 gnd rswk-swf Keimung (DE-588)4163559-0 gnd rswk-swf Solanum berthaultii (DE-588)4366117-8 gnd rswk-swf Pollen (DE-588)4046628-0 gnd rswk-swf Antisense-Nucleinsäuren (DE-588)4282992-6 gnd rswk-swf Transgene Pflanzen (DE-588)4311513-5 gnd rswk-swf Kartoffel (DE-588)4029804-8 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Solanum berthaultii (DE-588)4366117-8 s Pollenschlauch (DE-588)4277435-4 s Wachstum (DE-588)4064115-6 s Genexpression (DE-588)4020136-3 s DE-604 Pollen (DE-588)4046628-0 s Keimung (DE-588)4163559-0 s Kartoffel (DE-588)4029804-8 s Transgene Pflanzen (DE-588)4311513-5 s Inhibition (DE-588)4273127-6 s Antisense-Nucleinsäuren (DE-588)4282992-6 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006691335&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Liu, Jun-qi Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato Inhibition (DE-588)4273127-6 gnd Wachstum (DE-588)4064115-6 gnd Pollenschlauch (DE-588)4277435-4 gnd Keimung (DE-588)4163559-0 gnd Solanum berthaultii (DE-588)4366117-8 gnd Pollen (DE-588)4046628-0 gnd Antisense-Nucleinsäuren (DE-588)4282992-6 gnd Transgene Pflanzen (DE-588)4311513-5 gnd Kartoffel (DE-588)4029804-8 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4273127-6 (DE-588)4064115-6 (DE-588)4277435-4 (DE-588)4163559-0 (DE-588)4366117-8 (DE-588)4046628-0 (DE-588)4282992-6 (DE-588)4311513-5 (DE-588)4029804-8 (DE-588)4020136-3 (DE-588)4113937-9 |
| title | Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato |
| title_auth | Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato |
| title_exact_search | Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato |
| title_full | Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato vorgelegt von Jun-qi Liu |
| title_fullStr | Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato vorgelegt von Jun-qi Liu |
| title_full_unstemmed | Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato vorgelegt von Jun-qi Liu |
| title_short | Characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato |
| title_sort | characterization of genes expressed during pollen germination and tube growth and the antisense inhibition of gene expression in transgenic potato |
| topic | Inhibition (DE-588)4273127-6 gnd Wachstum (DE-588)4064115-6 gnd Pollenschlauch (DE-588)4277435-4 gnd Keimung (DE-588)4163559-0 gnd Solanum berthaultii (DE-588)4366117-8 gnd Pollen (DE-588)4046628-0 gnd Antisense-Nucleinsäuren (DE-588)4282992-6 gnd Transgene Pflanzen (DE-588)4311513-5 gnd Kartoffel (DE-588)4029804-8 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | Inhibition Wachstum Pollenschlauch Keimung Solanum berthaultii Pollen Antisense-Nucleinsäuren Transgene Pflanzen Kartoffel Genexpression Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006691335&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT liujunqi characterizationofgenesexpressedduringpollengerminationandtubegrowthandtheantisenseinhibitionofgeneexpressionintransgenicpotato |