Protein expression in animal cells:
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
San Diego [u.a.]
Acad. Press
1994
|
| Schriftenreihe: | Methods in cell biology
43 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 379 S. Ill., graph. Darst. |
| ISBN: | 0125641443 0125985606 |
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| 245 | 1 | 0 | |a Protein expression in animal cells |c ed. by Michael G. Roth |
| 264 | 1 | |a San Diego [u.a.] |b Acad. Press |c 1994 | |
| 300 | |a XVI, 379 S. |b Ill., graph. Darst. | ||
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| 650 | 7 | |a Cellen (biologie) |2 gtt | |
| 650 | 4 | |a Cellules | |
| 650 | 7 | |a Eiwitten |2 gtt | |
| 650 | 4 | |a Expression génique | |
| 650 | 7 | |a Genexpressie |2 gtt | |
| 650 | 4 | |a Protéines recombinantes | |
| 650 | 4 | |a Virus recombinants | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Animal cell biotechnology | |
| 650 | 4 | |a Gene Expression | |
| 650 | 4 | |a Protein Engineering |x methods | |
| 650 | 4 | |a Proteins |x Biotechnology | |
| 650 | 4 | |a Recombinant Proteins | |
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Datensatz im Suchindex
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|---|---|
| adam_text | Titel: Protein expression in animal cells
Autor: Roth, Michael G.
Jahr: 1994
CONTENTS
Contributors xi
Preface xv
PART I The Use of Naturally Occurring and
Recombinant Viruses
1. Viruses as Model Systems in Cell Biology
Richard W. Compans and Paul C. Roberts
I. Introduction 3
II. Cell Biology of Virus Infection 5
III. Methods for Virus Growth, Assay, and Purification 14
References 35
2. Expression of Exogenous Proteins in Mammalian Cells with the
Simliki Forest Virus Vector
Vesa M. Olkkonen, Peter Liljestrom, Paul Dupree, Henrik Garoff,
and Kai Simons
I. Introduction 43
II. Procedure 44
References 52
3. Recombinant Sindbis Virus as an Expression System for
Cell Biology
Robert C. Piper, Jan W. Slot, Guangpu Li, Phillip D. Staid,
and David E. James
I. Introduction 56
II. Sindbis Virus Biology 57
III. Previous Studies with Sindbis Virus Expression System 69
References 75
4. Recombinant Rous Sarcoma Virus Vectors for Avian Cells
Greg Odorizzi and Ian S. Troivbridge
I. Introduction 79
II. Replication-Competent Rous Sarcoma Virus Vectors 80
v
III. Expression of Human Transferrin Receptor in Chick
Embryo Fibroblasts 87
References 95
5. Generation of High-Titer Pseudotyped Retroviral Vectors with
Very Broad Host Range
Jiing-Kuan Yee, Theodore Friedtnann, and Jane C Burns
I. Introduction 99
II. Principle of Method 100
III. Methods 101
IV. Summary 110
References 111
6. SV40 Virus Expression Vectors
Hussein Y. Nairn and Michael G. Roth
I. Introduction 113
II. Uses of SV40 Virus Expression Vectors 115
III. Design of SV40 Expression Vectors 118
IV. Preparation of High-Titer Recombinant SV40 Virus Stocks 122
V. Analysis of the Efficiency of Protein Expression 127
References 135
7. Use of Recombinant Vaccinia Virus Vectors for
Cell Biology
Ora A. Weisz and Carolyn E. Machamer
I. Introduction 137
II. Generating a Recombinant Vaccinia Virus 142
III. Large-Scale Growth and Purification of Vaccinia Virus Stocks 147
IV. Vaccinia Virus-Mediated Protein Expression 149
V. Discussion 155
References 158
8. Use of Recombinant Adenovirus for Metabolic Engineering of
Mammalian Cells
Thomas C. Becker, Richard J. Noel, Ward S. Coats, A. M. Gomez-Foix,
TausifAlam, Robert D. Gerard, and Christopher B. Newgard
I. Historical Perspective on the Development and Application of Viral
Vectors for DNA Transfer 162
II. Examples of the Utility of Recombinant Adenovirus for Studies on
Metabolic Regulation 164
III. Adenovirus Biology 170
IV. Development of Adenovirus Vectors 172
V. Specific Procedures for Preparation of Recombinant Viruses 174
VI. Future Directions 183
VII. Conclusions 185
References 185
9. Amplicon-Based Herpes Simplex Virus Vectors
Dora Y. Ho
I. Introduction 191
II. Components of an Amplicon 193
III. Choice of Helper Virus 197
IV. Generation of DI Vectors 198
V. Gene Expression in Neurons 203
VI. Final Remark: Choosing between DI and Recombinant
HSV Vectors 205
References 206
10. Replication-Defective Recombinant Herpes Simplex
Virus Vectors
Paul A. Johnson and Theodore Friedmann
I. Introduction 211
II. Designing an HSV-1 Vector 217
III. Methods 221
IV. Prospects 227
References 227
PART II The Use of Expression Plasmids in Continuous Cell Lines
11. Cytomegalovirus Plasmid Vectors for Permanent Lines of
Polarized Epithelial Cells
Colleen B. Brewer
I. Introduction 233
II. Features of Some CMV Plasmid Vectors 234
III. Madin-Darby Canine Kidney Cell Lines 237
IV. LLC-PK1 Cell Lines 243
V. Summary 243
References 244
12. Inducible Protein Expression Using a
Glucocorticoid-Sensitive Vector
Robert P. Hirt, Nicolas Fasel, and Jean-Pierre Kraehenbuhl
I. Introduction 247
II. pLK-neo Inducible Expression Vector 249
III. Inducible Protein Expression in Epithelial Cells 250
IV. Inducible Protein Expression in Nonepithelial Cells 253
V. Perspectives 256
VI. Protocols 257
VII. Conclusions 259
References 259
13. Expression of Exogenous Proteins in Cells with
Regulated Secretory Pathways
R. A. Chavez, Y.-T. Chen, W. K. Schmidt, L. Cornell,
and Hsiao-Ping Moore
I. Introduction 263
II. Considerations for the Choice of Expression Systems 267
III. Plasmid-Based Expression 269
IV. Vaccinia Virus-Based Expression 277
V. Measurement of Transport through the Regulated Pathway 279
VI. Overview and Conclusions 285
References 286
14. Expression of Foreign Proteins in a
Human Neuronal System
David G. Cook, Virginia M.-Y. Lee, and Robert W. Doms
I. Introduction 289
II. NT2N Cells: A Model Human Neuronal System 292
III. Expression of Foreign Genes in NT2N Cells 298
IV. Summary 302
References 302
15. Homologous Recombination for Gene Replacement in
Mouse Cell Lines
Thomas E. Willnow and Joachim Herz
I. Introduction 306
II. Materials 308
III. Experimental Procedures: Gene Disruption and Derivation of Mice
and Cells 312
IV. Experimental Procedures: Analysis of Genetically Altered Cells
and Mice 327
V. Discussion 331
References 332
16. Regulation of Protein Activities by Fusion to
Steroid Binding Domains
Tiziana Mattioni, Jean-Francois Louvion, and Didier Picard
I. Introduction 335
II. Advantages and Limits 337
III. Designing and Using Fusion Proteins 340
IV. Examples 343
References 349
17. Gene Gun Transfection of Animal Cells and
Genetic Immunization
Stephen A. Johnston and De-chu Tang
I. Introduction 353
II. Details of Operation 355
III. Applications 359
IV. Genetic Immunization 363
V. Summary 364
References 365
Index 367
Volumes in Series 375
|
| any_adam_object | 1 |
| author2 | Roth, Michael G. |
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| dewey-hundreds | 600 - Technology (Applied sciences) 500 - Natural sciences and mathematics |
| dewey-ones | 660 - Chemical engineering 591 - Specific topics in natural history of animals |
| dewey-raw | 660/.63 591.876 |
| dewey-search | 660/.63 591.876 |
| dewey-sort | 3660 263 |
| dewey-tens | 660 - Chemical engineering 590 - Animals |
| discipline | Chemie / Pharmazie Biologie |
| format | Book |
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| series | Methods in cell biology |
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| spelling | Protein expression in animal cells ed. by Michael G. Roth San Diego [u.a.] Acad. Press 1994 XVI, 379 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 43 ADN Calcium gtt Cellen (biologie) gtt Cellules Eiwitten gtt Expression génique Genexpressie gtt Protéines recombinantes Virus recombinants Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Animal cell biotechnology Gene Expression Protein Engineering methods Proteins Biotechnology Recombinant Proteins Expressivität Genetik (DE-588)4276178-5 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Tierzelle (DE-588)4185509-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Tierzelle (DE-588)4185509-7 s Genexpression (DE-588)4020136-3 s DE-604 Proteine (DE-588)4076388-2 s Expressivität Genetik (DE-588)4276178-5 s Roth, Michael G. edt Methods in cell biology 43 (DE-604)BV002534878 43 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006552211&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein expression in animal cells Methods in cell biology ADN Calcium gtt Cellen (biologie) gtt Cellules Eiwitten gtt Expression génique Genexpressie gtt Protéines recombinantes Virus recombinants Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Animal cell biotechnology Gene Expression Protein Engineering methods Proteins Biotechnology Recombinant Proteins Expressivität Genetik (DE-588)4276178-5 gnd Proteine (DE-588)4076388-2 gnd Genexpression (DE-588)4020136-3 gnd Tierzelle (DE-588)4185509-7 gnd |
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| title | Protein expression in animal cells |
| title_auth | Protein expression in animal cells |
| title_exact_search | Protein expression in animal cells |
| title_full | Protein expression in animal cells ed. by Michael G. Roth |
| title_fullStr | Protein expression in animal cells ed. by Michael G. Roth |
| title_full_unstemmed | Protein expression in animal cells ed. by Michael G. Roth |
| title_short | Protein expression in animal cells |
| title_sort | protein expression in animal cells |
| topic | ADN Calcium gtt Cellen (biologie) gtt Cellules Eiwitten gtt Expression génique Genexpressie gtt Protéines recombinantes Virus recombinants Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Animal cell biotechnology Gene Expression Protein Engineering methods Proteins Biotechnology Recombinant Proteins Expressivität Genetik (DE-588)4276178-5 gnd Proteine (DE-588)4076388-2 gnd Genexpression (DE-588)4020136-3 gnd Tierzelle (DE-588)4185509-7 gnd |
| topic_facet | ADN Calcium Cellen (biologie) Cellules Eiwitten Expression génique Genexpressie Protéines recombinantes Virus recombinants Wetenschappelijke technieken Wissenschaftliches Arbeiten Animal cell biotechnology Gene Expression Protein Engineering methods Proteins Biotechnology Recombinant Proteins Expressivität Genetik Proteine Genexpression Tierzelle Aufsatzsammlung |
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