Tissue culture techniques: an introduction
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Boston [u.a.]
Birkhäuser
1994
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 247 S. Ill., graph. Darst. |
| ISBN: | 0817637184 3764337184 0817636439 3764336439 |
Internformat
MARC
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| 100 | 1 | |a Martin, Bernice M. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Tissue culture techniques |b an introduction |c Bernice M. Martin |
| 264 | 1 | |a Boston [u.a.] |b Birkhäuser |c 1994 | |
| 300 | |a X, 247 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 7 | |a Cell Culture |2 cabt | |
| 650 | 7 | |a Tissue Culture |2 cabt | |
| 650 | 7 | |a Techniques |2 cabt | |
| 650 | 7 | |a Biologia geral |2 larpcal | |
| 650 | 7 | |a Tissus (histologie) - Cultures et milieux de culture |2 ram | |
| 650 | 4 | |a Culture Techniques |v Laboratory Manuals | |
| 650 | 4 | |a Tissue culture | |
| 650 | 0 | 7 | |a Gewebekultur |0 (DE-588)4157245-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zellkultur |0 (DE-588)4067547-6 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1807774154358784000 |
|---|---|
| adam_text |
OENTENTS
PREFACE
.
ACKNOWLEDGMENTS
.
INTRODUCTION
.
STERILITY
.
ASEPTIC
TECHNIQUE
.
$
PHYSICAL
MANIPULATIONS
YY
USE
OFTHE
STERILE
CABINET
(HOOD)
STERILIZATION
METHODS
.
HEAT
YY
RADIATION
YY
TOXIC
GAS
YY
FILTRATION
YY
ANTIBIOTICS
QUALITY
CONTROL
OF
STERILIZATION
.
23
ROUTINE
LABELING
SUGGESTED
READINGS
.
25
EXERCISES
.
YY
CONTENTS
JU
1
INE
GELL
CULTURE
.
29
FEEDING
SCHEDULES
AND
MEDIA
COMPONENTS
.
29
GENERALPROPERTIES
OFMEDIA
AND
SAH
SOLUTIONS
YY
WATER
AS
A
REAGENT
YY
ESTABLISHINGFEEDING
SCHEDULES
SUBCULTIVATION
.
46
SOLUTIONS
AND
METHODS
FOR
ADHERENT
CELLS
YY
COMMON
ENZYME
SOLUTIONS
*
INOCULATING
(SEEDING)
THE
CULTURES
CELL
ENUMERATION
AND
CELL
VIABILITY
.
54
HEMOCYTOMETER
YY
PARTICLE
COUNTER
YY
CELL
VIABILITY
PUTTING
ROUTINE
METHODS
TO
WORK
.
63
NORMAL
CELL
GROWTH
CHARACTERISTICS
DETECTING
AND
DISPOSING
OF
CONTAMINATION
.
66
BACTERIA
ANDFIINGI
YY
FUNGI
YY
MYCOPLASMA
YY
VIRUSES
YY
DEALING
WITH
CONTAMINATION
TROUBLESHOOTING
.
73
INADEQUATE
CELL
GROWTH
YY
RECURRENT
CONTAMINATION
YY
WHEN
TO
CALLYOUR
VENDOR
SAFETY
.
80
BIOLOGICAL
HAZARDS
YY
CHEMICAL
HAZARDS
SUGGESTED
READINGS
.
85
PROBLEM
SET
.
85
EXERCISES
.
89
PERIMENTS
IN
CULTURE
.
91
ALTERATIONS
OF
THE
MEDIA
.
91
SERUM
YY
TREATMENTS
OF
SERUM
YY
PLASMA-DERIVED
SERUM
YY
SERUM-FREE
AND
LOW-PROTEIN
MEDIA
SUBSTRATA
.101
COATINGPLASTICWARE
WITH
SOLUTIONS
YY
ALTERATIONS
WITHPOLYMERS
*
USING
CELLS
TO
COAT
THE
PLASTICWARE
YY
CULTURING
CELLS
ON
MICROCARRIERS
ALTERING
THE
ENVIRONMENT
.
106
TEMPERATURE
CHANGES
*
GASEOUS
CHANGES
PROBLEM
SET
.
110
EXERCISES
.
110
CONTENTS
VII
PRIMARY
CELL
CULTURE
.
ISOLATION
.
DISSECTION
YY
ENZYMATIC
DISSOCIATION
METHODS
YY
NONENZYMATIC
ISOLATION
YY
PURIFICATION
OFCELL
SUSPENSIONS
YY
CONSIDERINGYIELD
AND
SURVIVAL
CHARACTERIZATION
.
PREPARATION
YY
FIXATION
ANDPERMEABILIZATION
YY
IDENTIFICATION
EXERCISE
.
CELL
PRESERVATION
.
FREEZING
THE
CELIS
.
THAWING
THE
CELIS
.
EQUIPMENT
YY
SOLUTIONS
PROBLEM
SET
.
CELLCLONING
.
ISOLATION
.
LIMITING
DILUTION
YY
CLONING
CYLINDERS
ASSISTING
CLONAL
SURVIVAL
.
USING
CONDITIONED
MEDIUM
YY
USINGFEEDER
LAYERS
PROBLEM
SET
.
CULTURE
CHANGES
.
THE
LOESS
OF
DIFFERENTIATED
PHENOTYPE
.
IMMORTALITY
AND
AGING
CELL
TRANSFORMATION
.
CHEMICAL
TRANSFORMATION
YY
VIRAL
TRANSFORMATION
YY
TRANSFORMED
CELLS
FROM
TUMORS
TRANSFECTION
(GENE
TRANSFER)
.
METHODS
YY
EVALUATION
SUGGESTED
READINGS
.
PROBLEM
SET
.
EXERCISES
.
154
155
158
181
182
183
VIII
CONTENTS
INFORMATION
FOR
NEW
CELL
STUDIES
.
185
SEARCHING
IN
THE
LITERATURE
.
185
USING
ADVERTISING
AND
CATALOGS
.
189
REFERENCES,
BIBLIOGRAPHIES,
AND
DATABASES
.
190
APPENDICES
THE
CELL
CYCLE
.
193
SUGGESTED
READINGS
.
196
MEDIA
AND
SALT
SOLUTIONS
.
197
MEDIA
.
197
E
E
E
SAIT
SOLUTIONS
.
199
VENDORS
.
207
GLOSSARY
.
223
ANSWERS
TO
PROBLEM
SETS
AND
EXERCISES
.
231
INDEX
.
243 |
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| ctrlnum | (OCoLC)29913214 (DE-599)BVBBV009866364 |
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| dewey-search | 574/.0724 |
| dewey-sort | 3574 3724 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-08-19T00:36:18Z |
| institution | BVB |
| isbn | 0817637184 3764337184 0817636439 3764336439 |
| language | English |
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| physical | X, 247 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| publisher | Birkhäuser |
| record_format | marc |
| spelling | Martin, Bernice M. Verfasser aut Tissue culture techniques an introduction Bernice M. Martin Boston [u.a.] Birkhäuser 1994 X, 247 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Cell Culture cabt Tissue Culture cabt Techniques cabt Biologia geral larpcal Tissus (histologie) - Cultures et milieux de culture ram Culture Techniques Laboratory Manuals Tissue culture Gewebekultur (DE-588)4157245-2 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf Zellkultur (DE-588)4067547-6 s DE-604 Gewebekultur (DE-588)4157245-2 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006533449&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Martin, Bernice M. Tissue culture techniques an introduction Cell Culture cabt Tissue Culture cabt Techniques cabt Biologia geral larpcal Tissus (histologie) - Cultures et milieux de culture ram Culture Techniques Laboratory Manuals Tissue culture Gewebekultur (DE-588)4157245-2 gnd Zellkultur (DE-588)4067547-6 gnd |
| subject_GND | (DE-588)4157245-2 (DE-588)4067547-6 |
| title | Tissue culture techniques an introduction |
| title_auth | Tissue culture techniques an introduction |
| title_exact_search | Tissue culture techniques an introduction |
| title_full | Tissue culture techniques an introduction Bernice M. Martin |
| title_fullStr | Tissue culture techniques an introduction Bernice M. Martin |
| title_full_unstemmed | Tissue culture techniques an introduction Bernice M. Martin |
| title_short | Tissue culture techniques |
| title_sort | tissue culture techniques an introduction |
| title_sub | an introduction |
| topic | Cell Culture cabt Tissue Culture cabt Techniques cabt Biologia geral larpcal Tissus (histologie) - Cultures et milieux de culture ram Culture Techniques Laboratory Manuals Tissue culture Gewebekultur (DE-588)4157245-2 gnd Zellkultur (DE-588)4067547-6 gnd |
| topic_facet | Cell Culture Tissue Culture Techniques Biologia geral Tissus (histologie) - Cultures et milieux de culture Culture Techniques Laboratory Manuals Tissue culture Gewebekultur Zellkultur |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006533449&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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