Fluorescent nucleotide derivatives as ligands for mitochondrial carriers:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
1994
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 127 S. Ill., graph. Darst. |
Internformat
MARC
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| 007 | t | ||
| 008 | 941017s1994 ad|| m||| 00||| engod | ||
| 016 | 7 | |a 942787498 |2 DE-101 | |
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| 100 | 1 | |a Huang, Shugui |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Fluorescent nucleotide derivatives as ligands for mitochondrial carriers |c von Shugui Huang |
| 264 | 1 | |c 1994 | |
| 300 | |a 127 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 1994 | ||
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Datensatz im Suchindex
| _version_ | 1807684211399720960 |
|---|---|
| adam_text |
CONTENT
1.
INTRODUCTION
1
2.
MATERIALS
AND
METHODS
4
2.1.
REAGENTS
AND
MATERIALS
4
2.2.
INSTRUMENTS
4
2.3.
EXPERIMENTAL
5
2.3.1.
PREPARATION
OF
THE
FLUORESCENT
NUCLEOTIDE
DERIVATIVES
5
2.3.2.
PREPARATION
OF
MITOCHONDRIA
6
2.3.3.
PREPARATION
OF
SUBMITOCHONDRIAL
PARTICLES
7
2.3.4.
ISOLATION
OF
CARRIERS
7
2.3.5.
PREPARATION
OF
UCP
PROTEOLIPOSOMES
9
2.3.6.
FLUORESCENCE
MEASUREMENT
10
2.3.7.
BINDING
TO BROWN
FAT
MITOCHONDRIA
11
2.3.8.
CHEMICAL
MODIFICATION
12
2.3.9.
LIMITED
PROTEOLYSIS
OF
UCP
13
2.3.10.
INHIBITION
OF
PROTON
TRANSPORT
ACTIVITY
13
2.3.11.
INTERACTION
WITH
KINASES
14
2.4.
THEORETICAL
14
2.4.1.
BINDING
14
2.4.2.
PH
DEPENDENCE
OF
DANS
NUCLEOTIDE
BINDING
16
3.
RESULTS
19
3.1.
PREPARATION
AND
PROPERTIES
OF
DANS
AND
DAN
NUCLEOTIDES
19
3.2.
BINDING
OF
DANS
NUCLEOTIDES
TO
TRITON
SOLUBILIZED
UCP
24
3.2.1.
BINDING
TO
THE
NUCLEOTIDE
BINDING
SITE
24
3.2.2.
PH
DEPENDENCE
OF
DANS
NUCLEOTIDE
BINDING
TO
UCP
30
3.2.3.
INFLUENCE
OF
IONS
AND
TEMPERATURE
ON
BINDING
37
3.2.4.
TRANSIENT
KINETICS
OF
BINDING
40
3.2.5.
FLUORESCENCE
ENERGY
TRANSFER
BETWEEN
UCP
TRYPTOPHAN
AND
DANS
NUCLEOTIDE
45
/
3.3.
DANS
NUCLEOTIDES
AS
FLUORESCENT
PROBES
FOR
STUDYING
INTERACTIONS
WITH
UCP
48
3.4.
MODIFICATION
OF
UCP WITH
AMINO
ACID
REAGENTS
53
3.5.
LIMITED
PROTEOLYSIS
OF
UCP
57
3.5.1.
TRYPSIN
57
3.5.2.
CHYMOTRYPSIN
60
3.6.
BINDING
OF
DANS
NUCLEOTIDES
TO
RECONSTITUTED
UCP
LIPOSOMES
66
3.7.
INHIBITION
OF
PROTON
TRANSPORT
OF
UCP
BY
DANS
NUCLEOTIDES
69
3.8.
BINDING
OF
DANS
NUCLEOTIDES
TO BROWN
FAT
MITOCHONDRIA
73
3.9.
INTERACTION
OF
DANS
AND
DAN
NUCLEOTIDES
WITH
ADP/ATP
CARRIERS
84
3.10.
INTERACTION
OF
DANS
AND
DAN
NUCLEOTIDES
WITH
KINASES
86
4.
DISCUSSION
88
4.1.
PROPERTIES
OF
THE
DANS
AND
DAN
NUCLEOTIDES
88
4.2.
BINDING
OF
DANS
NUCLEOTIDES
TO
UCP
89
4.3.
CONFORMATIONAL
CHANGE
OF
UCP
UPON
DANS
NUCLEOTIDE
BINDING
105
4.4.
PROTON
TRANSPORT
INHIBITION
BY
THE
DANS
NUCLEOTIDES
105
4.5.
MECHANISM
OF
BINDING
AND
INHIBITION 108
4.6.
ENDOGENOUS
ATP
IS
RESPONSIBLE
FOR
THE
MASKING
PHENOMENON
110
4.7.
INTERACTION
OF
THE
DANS
AND
DAN
NUCLEOTIDES
WITH
ADP/ATP
CARRIERS
AND
KINASES
112
5.
SUMMARY
115
6.
ABBREVIATIONS
118
7.
APPENDIX
120
7.1.
SEQUENCES
OF
THE
ADP/ATP
CARRIER
AND
UNCOUPLING
PROTEIN
120
7.2.
COUPLED
ENZYMATIC
JESTS
122
8.
REFERENCE
124 |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV009854955 |
| illustrated | Illustrated |
| indexdate | 2024-08-18T00:46:42Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006523808 |
| oclc_num | 257903335 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-29T DE-12 DE-83 DE-11 DE-188 |
| owner_facet | DE-19 DE-BY-UBM DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-29T DE-12 DE-83 DE-11 DE-188 |
| physical | 127 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| record_format | marc |
| spelling | Huang, Shugui Verfasser aut Fluorescent nucleotide derivatives as ligands for mitochondrial carriers von Shugui Huang 1994 127 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 1994 Carrier-Proteine (DE-588)4213127-3 gnd rswk-swf Mitochondrium (DE-588)4124939-2 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf Nucleotide (DE-588)4135085-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Mitochondrium (DE-588)4124939-2 s Carrier-Proteine (DE-588)4213127-3 s Nucleotide (DE-588)4135085-6 s Wechselwirkung (DE-588)4064937-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006523808&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Huang, Shugui Fluorescent nucleotide derivatives as ligands for mitochondrial carriers Carrier-Proteine (DE-588)4213127-3 gnd Mitochondrium (DE-588)4124939-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Nucleotide (DE-588)4135085-6 gnd |
| subject_GND | (DE-588)4213127-3 (DE-588)4124939-2 (DE-588)4064937-4 (DE-588)4135085-6 (DE-588)4113937-9 |
| title | Fluorescent nucleotide derivatives as ligands for mitochondrial carriers |
| title_auth | Fluorescent nucleotide derivatives as ligands for mitochondrial carriers |
| title_exact_search | Fluorescent nucleotide derivatives as ligands for mitochondrial carriers |
| title_full | Fluorescent nucleotide derivatives as ligands for mitochondrial carriers von Shugui Huang |
| title_fullStr | Fluorescent nucleotide derivatives as ligands for mitochondrial carriers von Shugui Huang |
| title_full_unstemmed | Fluorescent nucleotide derivatives as ligands for mitochondrial carriers von Shugui Huang |
| title_short | Fluorescent nucleotide derivatives as ligands for mitochondrial carriers |
| title_sort | fluorescent nucleotide derivatives as ligands for mitochondrial carriers |
| topic | Carrier-Proteine (DE-588)4213127-3 gnd Mitochondrium (DE-588)4124939-2 gnd Wechselwirkung (DE-588)4064937-4 gnd Nucleotide (DE-588)4135085-6 gnd |
| topic_facet | Carrier-Proteine Mitochondrium Wechselwirkung Nucleotide Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006523808&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT huangshugui fluorescentnucleotidederivativesasligandsformitochondrialcarriers |