Electrophoresis of enzymes: laboratory methods
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Berlin [u.a.]
Springer
1994
|
| Series: | Springer lab manual
|
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Physical Description: | XII, 307 S. Ill., graph. Darst. |
| ISBN: | 3540581146 0387581146 |
Staff View
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV009790341 | ||
| 003 | DE-604 | ||
| 005 | 20220704 | ||
| 007 | t| | ||
| 008 | 940829s1994 gw ad|| |||| 00||| eng d | ||
| 016 | 7 | |a 941901211 |2 DE-101 | |
| 020 | |a 3540581146 |9 3-540-58114-6 | ||
| 020 | |a 0387581146 |9 0-387-58114-6 | ||
| 035 | |a (OCoLC)844925075 | ||
| 035 | |a (DE-599)BVBBV009790341 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 |a DE-703 |a DE-91G |a DE-20 |a DE-M49 |a DE-634 |a DE-83 |a DE-188 |a DE-11 | ||
| 084 | |a WD 5050 |0 (DE-625)148185: |2 rvk | ||
| 084 | |a WC 3440 |0 (DE-625)148085: |2 rvk | ||
| 084 | |a WC 4350 |0 (DE-625)148095: |2 rvk | ||
| 084 | |a YV 3400 |0 (DE-625)154222:12905 |2 rvk | ||
| 084 | |a CHE 228f |2 stub | ||
| 084 | |a CHE 810f |2 stub | ||
| 084 | |a CHE 828f |2 stub | ||
| 100 | 1 | |a Rothe, Gunter M. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Electrophoresis of enzymes |b laboratory methods |c G. M. Rothe |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 1994 | |
| 300 | |a XII, 307 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Springer lab manual | |
| 650 | 7 | |a Enzymes |2 cabt | |
| 650 | 7 | |a Electrophoresis |2 cabt | |
| 650 | 7 | |a Laboratory Methods |2 cabt | |
| 650 | 0 | 7 | |a Elektrophorese |0 (DE-588)4014373-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Enzym |0 (DE-588)4014988-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Isoenzym |0 (DE-588)4125489-2 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Enzym |0 (DE-588)4014988-2 |D s |
| 689 | 0 | 1 | |a Elektrophorese |0 (DE-588)4014373-9 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Isoenzym |0 (DE-588)4125489-2 |D s |
| 689 | 1 | 1 | |a Elektrophorese |0 (DE-588)4014373-9 |D s |
| 689 | 1 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006477457&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-006477457 | |
Record in the Search Index
| _version_ | 1829371800173150208 |
|---|---|
| adam_text |
I
INTRODUCTION
.
I
1.1
ENZYME
CLASSIFICATION
.
I
1.2
ENZYME
SUBUNIT
COMPOSITION
.
3
1.3
ISOZYME
CLASSIFICATION
.
14
1.4
REFERENCES
.
18
2
EXTRACTION
OF
ENZYMES
FROM
TISSUES,
CELLS
AND
CELL-ORGANELLES
.
19
2.1
METHODS
TO
EXTRACT
ENZYMES
FROM
MICROORGANISMS
.
19
2.2
METHODS
TO
EXTRACT
ENZYMES
FROM
ANIMAL
SOFT
TISSUE
.
22
2.3
DIFFERENTIAL
EXTRACTION
OF
CYTOSOLIC
AND
MITOCHONDRIAL
ENZYMES
FROM
ANIMAL
SOFT
TISSUE
.
26
2.4
EXTRACTION
OF
INSECTS
.
28
2.5
EXTRACTION
OF
PLANT
TISSUES
.
29
2.6
CONCENTRATION
OF
DILUTED
ENZYME
EXTRACTS
.
31
2.7
MAMMALIAN
BLOOD
.
34
2.8
PANCREATIC
ISLETS
.
42
2.9
ISOLATION
OF
SUBCELLULAR
ORGANELLES
.
43
2.10
CATALYTIC
ACTIVITIES
OF
ENZYMES
FORMING
A
COMMON
METABOLIC
SEQUENCE
.
65
2.11
REFERENCES
.
67
3
METHODS
FOR
SEPARATING
NATIVE
ENZYMES
.
71
3.1
GENERAL
CONSIDERATIONS
.
7
1
3.2
CELLULOSE
ACETATE
ELECTROPHORESIS
.
72
3.3
STARCH
GEL
ELECTROPHORESIS
.
80
3.4
REFERENCES
.
88
3.5
ANALYTICAL
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
FOR
SEPARATING
NATIVE
ENZYMES
.
89
3.6
2-D-ELECTROPHORESIS
UNDER
NON-DENATURING
CONDITIONS
.
120
3.7
REFERENCES
.
124
4
SODIUM
DODECYLSULPHATE
ELECTROPHORESIS
.
127
4.1
HOMOGENEOUS
BUFFER
SYSTEMS
.
127
4.2
DISCONTINUOUS
BUFFER
SYSTEMS
.
128
4.3
GRADIENT
GEL
SYSTEMS
.
130
XII
CONTENTS
4.4
ENZYME
VISUALIZATION
FOLLOWING
SDS-ELECTROPHORESIS
.
134
4.5
REFERENCES
.
138
5
CHEMISTRY
OF
ENZYME
VISUALIZATION
.
141
5.1
METHODS
FOR
VISUALIZING
OXIDATIVE
ENZYMES
.
142
5.2
METHODS
FOR
VISUALIZING
TRANSFERASES
.
151
5.3
METHODS
FOR
VISUALIZING
HYDROLASES
.
155
5.4
METHODS
FOR
VISUALIZING
LYASES
.
167
5.5
METHODS
FOR
LOCALIZING
ISOMERASES
AND
LIGASES
.
168
5.6
VISUALIZATION
OF
ENZYMES
BY
DIFFERENT
METHODS
.
168
5.7
REFERENCES
.
176
6
A
COMPILATION
OF
PROTOCOLS
TO
VISUALIZE
ENZYMES
.
181
6.1
INDEX
TO
ENZYME
RECIPES
AND
SEPARATION
SYSTEMS
.
181
6.2
PROTOCOLS
TO
VISUALIZE
ENZYMES
FOLLOWING
ELECTROPHORETIC
SEPARATION
.
.
.
186
6.3
REFERENCES
.
268
7
DATA
EVALUATION
IN
POPULATION
GENETICS
AND
EVOLUTION
.
273
7.1
ALLOZYMES
AS
GENE
MARKERS
.
273
7.2
POPULATION
GENETIC
MEASURES
.
277
7.3
CALCULATION
OF
DENDROGRAMS
.
295
7.4
REFERENCES
.
300
SUBJECT
INDEX
.
303 |
| any_adam_object | 1 |
| author | Rothe, Gunter M. |
| author_facet | Rothe, Gunter M. |
| author_role | aut |
| author_sort | Rothe, Gunter M. |
| author_variant | g m r gm gmr |
| building | Verbundindex |
| bvnumber | BV009790341 |
| classification_rvk | WD 5050 WC 3440 WC 4350 YV 3400 |
| classification_tum | CHE 228f CHE 810f CHE 828f |
| ctrlnum | (OCoLC)844925075 (DE-599)BVBBV009790341 |
| discipline | Biologie Chemie Medizin |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV009790341</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20220704</controlfield><controlfield tag="007">t|</controlfield><controlfield tag="008">940829s1994 gw ad|| |||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">941901211</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">3540581146</subfield><subfield code="9">3-540-58114-6</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0387581146</subfield><subfield code="9">0-387-58114-6</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)844925075</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV009790341</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-703</subfield><subfield code="a">DE-91G</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-M49</subfield><subfield code="a">DE-634</subfield><subfield code="a">DE-83</subfield><subfield code="a">DE-188</subfield><subfield code="a">DE-11</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WD 5050</subfield><subfield code="0">(DE-625)148185:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 3440</subfield><subfield code="0">(DE-625)148085:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4350</subfield><subfield code="0">(DE-625)148095:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">YV 3400</subfield><subfield code="0">(DE-625)154222:12905</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 228f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 810f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 828f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Rothe, Gunter M.</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Electrophoresis of enzymes</subfield><subfield code="b">laboratory methods</subfield><subfield code="c">G. M. Rothe</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Berlin [u.a.]</subfield><subfield code="b">Springer</subfield><subfield code="c">1994</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XII, 307 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Springer lab manual</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Enzymes</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Electrophoresis</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Laboratory Methods</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Elektrophorese</subfield><subfield code="0">(DE-588)4014373-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Enzym</subfield><subfield code="0">(DE-588)4014988-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Isoenzym</subfield><subfield code="0">(DE-588)4125489-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Enzym</subfield><subfield code="0">(DE-588)4014988-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Elektrophorese</subfield><subfield code="0">(DE-588)4014373-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Isoenzym</subfield><subfield code="0">(DE-588)4125489-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Elektrophorese</subfield><subfield code="0">(DE-588)4014373-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006477457&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-006477457</subfield></datafield></record></collection> |
| id | DE-604.BV009790341 |
| illustrated | Illustrated |
| indexdate | 2025-04-14T10:01:40Z |
| institution | BVB |
| isbn | 3540581146 0387581146 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006477457 |
| oclc_num | 844925075 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-703 DE-91G DE-BY-TUM DE-20 DE-M49 DE-BY-TUM DE-634 DE-83 DE-188 DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-703 DE-91G DE-BY-TUM DE-20 DE-M49 DE-BY-TUM DE-634 DE-83 DE-188 DE-11 |
| physical | XII, 307 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| publisher | Springer |
| record_format | marc |
| series2 | Springer lab manual |
| spelling | Rothe, Gunter M. Verfasser aut Electrophoresis of enzymes laboratory methods G. M. Rothe Berlin [u.a.] Springer 1994 XII, 307 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer lab manual Enzymes cabt Electrophoresis cabt Laboratory Methods cabt Elektrophorese (DE-588)4014373-9 gnd rswk-swf Enzym (DE-588)4014988-2 gnd rswk-swf Isoenzym (DE-588)4125489-2 gnd rswk-swf Enzym (DE-588)4014988-2 s Elektrophorese (DE-588)4014373-9 s DE-604 Isoenzym (DE-588)4125489-2 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006477457&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rothe, Gunter M. Electrophoresis of enzymes laboratory methods Enzymes cabt Electrophoresis cabt Laboratory Methods cabt Elektrophorese (DE-588)4014373-9 gnd Enzym (DE-588)4014988-2 gnd Isoenzym (DE-588)4125489-2 gnd |
| subject_GND | (DE-588)4014373-9 (DE-588)4014988-2 (DE-588)4125489-2 |
| title | Electrophoresis of enzymes laboratory methods |
| title_auth | Electrophoresis of enzymes laboratory methods |
| title_exact_search | Electrophoresis of enzymes laboratory methods |
| title_full | Electrophoresis of enzymes laboratory methods G. M. Rothe |
| title_fullStr | Electrophoresis of enzymes laboratory methods G. M. Rothe |
| title_full_unstemmed | Electrophoresis of enzymes laboratory methods G. M. Rothe |
| title_short | Electrophoresis of enzymes |
| title_sort | electrophoresis of enzymes laboratory methods |
| title_sub | laboratory methods |
| topic | Enzymes cabt Electrophoresis cabt Laboratory Methods cabt Elektrophorese (DE-588)4014373-9 gnd Enzym (DE-588)4014988-2 gnd Isoenzym (DE-588)4125489-2 gnd |
| topic_facet | Enzymes Electrophoresis Laboratory Methods Elektrophorese Enzym Isoenzym |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006477457&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT rothegunterm electrophoresisofenzymeslaboratorymethods |