Electrophoresis of enzymes: laboratory methods
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
1994
|
| Schriftenreihe: | Springer lab manual
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 307 S. Ill., graph. Darst. |
| ISBN: | 3540581146 0387581146 |
Internformat
MARC
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| 100 | 1 | |a Rothe, Gunter M. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Electrophoresis of enzymes |b laboratory methods |c G. M. Rothe |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 1994 | |
| 300 | |a XII, 307 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 490 | 0 | |a Springer lab manual | |
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| 650 | 7 | |a Electrophoresis |2 cabt | |
| 650 | 7 | |a Laboratory Methods |2 cabt | |
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| 650 | 0 | 7 | |a Enzym |0 (DE-588)4014988-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Isoenzym |0 (DE-588)4125489-2 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1829371800173150208 |
|---|---|
| adam_text |
I
INTRODUCTION
.
I
1.1
ENZYME
CLASSIFICATION
.
I
1.2
ENZYME
SUBUNIT
COMPOSITION
.
3
1.3
ISOZYME
CLASSIFICATION
.
14
1.4
REFERENCES
.
18
2
EXTRACTION
OF
ENZYMES
FROM
TISSUES,
CELLS
AND
CELL-ORGANELLES
.
19
2.1
METHODS
TO
EXTRACT
ENZYMES
FROM
MICROORGANISMS
.
19
2.2
METHODS
TO
EXTRACT
ENZYMES
FROM
ANIMAL
SOFT
TISSUE
.
22
2.3
DIFFERENTIAL
EXTRACTION
OF
CYTOSOLIC
AND
MITOCHONDRIAL
ENZYMES
FROM
ANIMAL
SOFT
TISSUE
.
26
2.4
EXTRACTION
OF
INSECTS
.
28
2.5
EXTRACTION
OF
PLANT
TISSUES
.
29
2.6
CONCENTRATION
OF
DILUTED
ENZYME
EXTRACTS
.
31
2.7
MAMMALIAN
BLOOD
.
34
2.8
PANCREATIC
ISLETS
.
42
2.9
ISOLATION
OF
SUBCELLULAR
ORGANELLES
.
43
2.10
CATALYTIC
ACTIVITIES
OF
ENZYMES
FORMING
A
COMMON
METABOLIC
SEQUENCE
.
65
2.11
REFERENCES
.
67
3
METHODS
FOR
SEPARATING
NATIVE
ENZYMES
.
71
3.1
GENERAL
CONSIDERATIONS
.
7
1
3.2
CELLULOSE
ACETATE
ELECTROPHORESIS
.
72
3.3
STARCH
GEL
ELECTROPHORESIS
.
80
3.4
REFERENCES
.
88
3.5
ANALYTICAL
POLYACRYLAMIDE
GEL
ELECTROPHORESIS
FOR
SEPARATING
NATIVE
ENZYMES
.
89
3.6
2-D-ELECTROPHORESIS
UNDER
NON-DENATURING
CONDITIONS
.
120
3.7
REFERENCES
.
124
4
SODIUM
DODECYLSULPHATE
ELECTROPHORESIS
.
127
4.1
HOMOGENEOUS
BUFFER
SYSTEMS
.
127
4.2
DISCONTINUOUS
BUFFER
SYSTEMS
.
128
4.3
GRADIENT
GEL
SYSTEMS
.
130
XII
CONTENTS
4.4
ENZYME
VISUALIZATION
FOLLOWING
SDS-ELECTROPHORESIS
.
134
4.5
REFERENCES
.
138
5
CHEMISTRY
OF
ENZYME
VISUALIZATION
.
141
5.1
METHODS
FOR
VISUALIZING
OXIDATIVE
ENZYMES
.
142
5.2
METHODS
FOR
VISUALIZING
TRANSFERASES
.
151
5.3
METHODS
FOR
VISUALIZING
HYDROLASES
.
155
5.4
METHODS
FOR
VISUALIZING
LYASES
.
167
5.5
METHODS
FOR
LOCALIZING
ISOMERASES
AND
LIGASES
.
168
5.6
VISUALIZATION
OF
ENZYMES
BY
DIFFERENT
METHODS
.
168
5.7
REFERENCES
.
176
6
A
COMPILATION
OF
PROTOCOLS
TO
VISUALIZE
ENZYMES
.
181
6.1
INDEX
TO
ENZYME
RECIPES
AND
SEPARATION
SYSTEMS
.
181
6.2
PROTOCOLS
TO
VISUALIZE
ENZYMES
FOLLOWING
ELECTROPHORETIC
SEPARATION
.
.
.
186
6.3
REFERENCES
.
268
7
DATA
EVALUATION
IN
POPULATION
GENETICS
AND
EVOLUTION
.
273
7.1
ALLOZYMES
AS
GENE
MARKERS
.
273
7.2
POPULATION
GENETIC
MEASURES
.
277
7.3
CALCULATION
OF
DENDROGRAMS
.
295
7.4
REFERENCES
.
300
SUBJECT
INDEX
.
303 |
| any_adam_object | 1 |
| author | Rothe, Gunter M. |
| author_facet | Rothe, Gunter M. |
| author_role | aut |
| author_sort | Rothe, Gunter M. |
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| bvnumber | BV009790341 |
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| classification_tum | CHE 228f CHE 810f CHE 828f |
| ctrlnum | (OCoLC)844925075 (DE-599)BVBBV009790341 |
| discipline | Biologie Chemie Medizin |
| format | Book |
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| id | DE-604.BV009790341 |
| illustrated | Illustrated |
| indexdate | 2025-04-14T10:01:40Z |
| institution | BVB |
| isbn | 3540581146 0387581146 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006477457 |
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| physical | XII, 307 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| publisher | Springer |
| record_format | marc |
| series2 | Springer lab manual |
| spelling | Rothe, Gunter M. Verfasser aut Electrophoresis of enzymes laboratory methods G. M. Rothe Berlin [u.a.] Springer 1994 XII, 307 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer lab manual Enzymes cabt Electrophoresis cabt Laboratory Methods cabt Elektrophorese (DE-588)4014373-9 gnd rswk-swf Enzym (DE-588)4014988-2 gnd rswk-swf Isoenzym (DE-588)4125489-2 gnd rswk-swf Enzym (DE-588)4014988-2 s Elektrophorese (DE-588)4014373-9 s DE-604 Isoenzym (DE-588)4125489-2 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006477457&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Rothe, Gunter M. Electrophoresis of enzymes laboratory methods Enzymes cabt Electrophoresis cabt Laboratory Methods cabt Elektrophorese (DE-588)4014373-9 gnd Enzym (DE-588)4014988-2 gnd Isoenzym (DE-588)4125489-2 gnd |
| subject_GND | (DE-588)4014373-9 (DE-588)4014988-2 (DE-588)4125489-2 |
| title | Electrophoresis of enzymes laboratory methods |
| title_auth | Electrophoresis of enzymes laboratory methods |
| title_exact_search | Electrophoresis of enzymes laboratory methods |
| title_full | Electrophoresis of enzymes laboratory methods G. M. Rothe |
| title_fullStr | Electrophoresis of enzymes laboratory methods G. M. Rothe |
| title_full_unstemmed | Electrophoresis of enzymes laboratory methods G. M. Rothe |
| title_short | Electrophoresis of enzymes |
| title_sort | electrophoresis of enzymes laboratory methods |
| title_sub | laboratory methods |
| topic | Enzymes cabt Electrophoresis cabt Laboratory Methods cabt Elektrophorese (DE-588)4014373-9 gnd Enzym (DE-588)4014988-2 gnd Isoenzym (DE-588)4125489-2 gnd |
| topic_facet | Enzymes Electrophoresis Laboratory Methods Elektrophorese Enzym Isoenzym |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006477457&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT rothegunterm electrophoresisofenzymeslaboratorymethods |