Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
1994
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VII, 136 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
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| 001 | BV009751946 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 940803s1994 gw ad|| m||| 00||| eng d | ||
| 016 | 7 | |a 941358526 |2 DE-101 | |
| 035 | |a (OCoLC)52129782 | ||
| 035 | |a (DE-599)BVBBV009751946 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-91 |a DE-11 |a DE-188 | ||
| 100 | 1 | |a Stella, Maria Cristina |d 1965- |e Verfasser |0 (DE-588)122659848 |4 aut | |
| 245 | 1 | 0 | |a Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster |c vorgelegt von Maria Cristina Stella |
| 264 | 1 | |c 1994 | |
| 300 | |a VII, 136 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Tübingen, Univ., Diss., 1994 | ||
| 650 | 0 | 7 | |a Gelsolin |0 (DE-588)4325773-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a cDNS |0 (DE-588)4212297-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Taufliege |0 (DE-588)4078168-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Genexpression |0 (DE-588)4020136-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Taufliege |0 (DE-588)4078168-9 |D s |
| 689 | 0 | 1 | |a Gelsolin |0 (DE-588)4325773-2 |D s |
| 689 | 0 | 2 | |a cDNS |0 (DE-588)4212297-1 |D s |
| 689 | 0 | 3 | |a Genexpression |0 (DE-588)4020136-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006449984&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-006449984 | |
Datensatz im Suchindex
| _version_ | 1807324147410272256 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
1
SUMMARY
.
1
2
INTRODUCTION
.
4
THE
CYTOSKELETON
.
5
THE
CYTOSKELETON
IN
DROSOPHILA
MELANOGASTER
.
5
ACTIN
.
6
ACTIN
IN
DROSOPHILA
.
7
ACTIN
BINDING
PROTEINS
.
8
ACTIN
MONOMER
BINDING
PROTEINS
.
9
PROFILIN
IN
DROSOPHILA
.
10
CAPPING
PROTEINS
.
10
SEVERING
PROTEINS
.
11
SEVERING
PROTEINS
IN
DROSOPHILA
.
12
GELSOLIN
.
13
PROTEINS
THAT
BIND
TO
THE
SIDES
OF
ACTIN
FILAMENTS
.
16
PROTEINS
THAT
BIND
ONLY
ONE
FILAMENT
OF
ACTIN
.
16
TROPOMYOSINS
IN
DROSOPHILA
.
17
GELATION
PROTEINS
.
17
A-ACTININ
AND
SPECTRIN
IN
DROSOPHILA
.
18
BUNDLING
PROTEINS
.
19
PROTEINS
THAT
CAN
MOVE
ALONG
THE
ACTIN
FILAMENT
.
19
MYOSINS
IN
DROSOPHILA
.
19
CYTOSKELETON
AND
DROSOPHILA
DEVELOPMENT
.
20
CYTOSKELETON
IN
DROSOPHILA
OVARIES
.
28
3
MATERIALS
AND
METHODS
.
32
3.1
FLY
STOCKS
.
33
3.2
MOLECULAR
BIOLOGY
TECHNIQUES
.
33
3.2.1
SOUTHERN
BLOTS
.
33
3.2.2
MRNA
PREPARATION
AND
NORTHERN
BLOTS
.
33
3.2.3
HYBRIDIZATION
TO
SOUTHERN
OR
NORTHERN
BLOTS
.
34
3.2.4
FIRST
STRAND
OF
CDNA
.
34
3.2.5
AMPLIFICATION
OF
CDNA
GENERATED
BY
REVERSE
TRANSCRIPTASE
.
35
3.2.6
PCR
ANALYSIS
OF
SINGLE
EMBRYO
.
35
3.2.7
DNA
SEQUENCING
.
36
3.2.8
IDENTIFICATION
OF
THE
SPLICE
SITES
.
37
3.3
PROTEIN
TECHNIQUES
.
38
3.3.1
TRANSLATION
AND
TRANSCRIPTION
REACTIONS
.
38
3.3.2
PROTEIN
EXTRACTION
AND
WESTERN
BLOTS
.
39
3.3.3
ANTIBODY
PRODUCTION
AND
PURIFICATION
.
40
3.3.4
ELUTION
OF
ANTIBODIES
FROM
NITROCELLULOSE
PAPER
.
40
3.4
IMMUNOHISTOCHEMISTRY
.
41
3.4.1
IN
SITU
HYBRIDIZATION
ON
EMBRYOS
.
41
3.4.2
STAINING
ON
EMBRYOS,
LARVAE
AND
IMAGINAL
DISCS
.
41
3.4.3
IN
SITU
HYBRIDIZATION
TO
POLYTENE
CHROMOSOMES
.
42
4
RESULTS
.
43
CLONING
OF
GELSOLIN
.
44
INTRODUCTION
.
44
LOW
STRINGENCY
SCREENING
.
44
PCR
AMPLIFICATION
.
44
SOUTHERN
BLOT
ANALYSIS
OF
DROSOPHILA
GENOMIC
DNA
.
50
ISOLATION
OF
FULL
LENGTH
CDNAS
.
50
LOW
STRINGENCY
HYBRIDIZATIONS
ON
GENOMIC
SOUTHERN
BLOTS
.
51
NORTHERN
BLOT
ANALYSIS
.
53
STRUCTURE
OF
CLONE
A
AND
CLONE
B
.
54
MAP
OF
THE
GENOMIC
REGION
CONTAINING
GELSOLIN
.
57
MAPPING
OF
OTHER
TRANSCRIPTION
UNITS
PRESENT
IN
THE
GENOMIC
.
REGION
CONTAINING
GELSOLIN
.
59
DIFFERENTIAL
SPLICING
OF
DROSOPHILA
GELSOLIN
.
60
SECRETION
IN
VIVO
AND
IN
VITRO
OF
DROSOPHILA
GELSOLIN
.
61
EXPRESSION
OF
DROSOPHILA
GELSOLIN
.
63
ANTIBODY
PRODUCTION
AND
PURIFICATION
.
63
WESTERN
BLOT
ANALYSIS
OF
THE
EXPRESSION
OF
DROSOPHILA
GELSOLIN
.
66
GELSOLIN
LOCALIZATION
ON
EMBRYOS
.
68
GELSOLIN
LOCALIZATION
ON
IMAGINAL
DISCS
.
74
EMBRYONIC
LOCALIZATION
OF
GELSOLIN
TRANSCRIPTS
.
75
IN
SITU
HYBRIDIZATIONS
ON
EMBRYOS
LACKING
MESODERM
.
82
LOCALIZATION
OF
DROSOPHILA
GELSOLIN
IN
OVARIES
.
84
GENETIC
MAPPING
OF
GELSOLIN
.
87
LOCALIZATION
OF
GELSOLIN
ON
POLYTENE
CHROMOSOMES
.
87
DISTRIBUTION
OF
GELSOLIN
TRANSCRIPTS
AND
PROTEIN
IN
EMBRYOS
.
HOMOZYGOUS
FOR
DF(3R)A321
AND
DF(3R)XM3
.
91
P-ELEMENT
MUTAGENESIS
.
93
Y-RAYS
MUTATIONS
.
96
5
DISCUSSION
.
98
INTRODUCTION
.
99
CLONING
OF
DROSOPHILA
GELSOLIN
.
99
SOUTHERN
BLOT
ANALYSIS
.
101
COMPARISON
BETWEEN
DROSOPHILA
GELSOLIN
AND
OTHER
SEVERING
.
PROTEINS
.
101
IN
VITRO
BEHAVIOUR
OF
DROSOPHILA
GELSOLIN
.
102
IN
VIVO
EXPRESSION
OF
DROSOPHILA
GELSOLIN
.
104
LOCALIZATION
OF
GELSOLIN
TRANSCRIPTS
.
107
COMPARISON
WITH
HUMAN
GELSOLIN
.109
EVOLUTION
OF
GELSOLIN
.109
GENETIC
CHARACTERIZATION
OF
THE
GELSOLIN
LOCUS
.112
6
REFERENCES
.
115 |
| any_adam_object | 1 |
| author | Stella, Maria Cristina 1965- |
| author_GND | (DE-588)122659848 |
| author_facet | Stella, Maria Cristina 1965- |
| author_role | aut |
| author_sort | Stella, Maria Cristina 1965- |
| author_variant | m c s mc mcs |
| building | Verbundindex |
| bvnumber | BV009751946 |
| ctrlnum | (OCoLC)52129782 (DE-599)BVBBV009751946 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV009751946 |
| illustrated | Illustrated |
| indexdate | 2024-08-14T01:23:40Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006449984 |
| oclc_num | 52129782 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-11 DE-188 |
| owner_facet | DE-91 DE-BY-TUM DE-11 DE-188 |
| physical | VII, 136 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| record_format | marc |
| spelling | Stella, Maria Cristina 1965- Verfasser (DE-588)122659848 aut Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster vorgelegt von Maria Cristina Stella 1994 VII, 136 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Tübingen, Univ., Diss., 1994 Gelsolin (DE-588)4325773-2 gnd rswk-swf cDNS (DE-588)4212297-1 gnd rswk-swf Taufliege (DE-588)4078168-9 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Taufliege (DE-588)4078168-9 s Gelsolin (DE-588)4325773-2 s cDNS (DE-588)4212297-1 s Genexpression (DE-588)4020136-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006449984&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Stella, Maria Cristina 1965- Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster Gelsolin (DE-588)4325773-2 gnd cDNS (DE-588)4212297-1 gnd Taufliege (DE-588)4078168-9 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4325773-2 (DE-588)4212297-1 (DE-588)4078168-9 (DE-588)4020136-3 (DE-588)4113937-9 |
| title | Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster |
| title_auth | Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster |
| title_exact_search | Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster |
| title_full | Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster vorgelegt von Maria Cristina Stella |
| title_fullStr | Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster vorgelegt von Maria Cristina Stella |
| title_full_unstemmed | Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster vorgelegt von Maria Cristina Stella |
| title_short | Cloning and genetic characterization of a gelsolin homologue in Drosophila melanogaster |
| title_sort | cloning and genetic characterization of a gelsolin homologue in drosophila melanogaster |
| topic | Gelsolin (DE-588)4325773-2 gnd cDNS (DE-588)4212297-1 gnd Taufliege (DE-588)4078168-9 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | Gelsolin cDNS Taufliege Genexpression Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006449984&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT stellamariacristina cloningandgeneticcharacterizationofagelsolinhomologueindrosophilamelanogaster |