Gene targeting on the Ig loci: analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
1993
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Köln, Univ., Diss., 1994 |
| Beschreibung: | 120 S. Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Zou, Yong-Rui |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Gene targeting on the Ig loci |b analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies |c vorgelegt von Yong-Rui Zou |
| 264 | 1 | |c 1993 | |
| 300 | |a 120 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Köln, Univ., Diss., 1994 | ||
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Datensatz im Suchindex
| _version_ | 1807502927381659648 |
|---|---|
| adam_text |
CONTENTS
A.
INTRODUCTION
1
AL
.
ANTIBODY
STRUCTURE
AND
FUNCTION
1
A2.
THE
EXPRESSION
OF
IMMUNOGLOBULIN GENE
DURING
B
CELL
3
DEVELOPMENT
A3.
THE
MAKING
OF
HUMANIZED
ANTIBODIES
9
B.
MATERIALS
AND
METHODS
12
BL.
METHODS
OF
MOLECULAR
BIOLOGY
13
BL.L
TRANSFORMATION
OF
BACTERIA
13
BL
.2
PREPARATION
OF
PLASMID
DNA
13
BL
.3
ISOLATION
OF
LAMBDA
PHAGE
DNA
14
BL.
4
MOLECULAR
CLONING
OF
THE
TARGETING
CONSTRUCTS
14
BL.
4.1
CONSTRUCTION
OF
THE
C
K
T
TARGETING
VECTOR
16
BL.
4.2
CONSTRUCTION
OF
THE
C
K
R
TARGETING
VECTOR
18
BL.
4.3
CONSTRUCTION
OF
THE
CYIR TARGETING
VECTOR
21
BL
.5
DNA
SEQUENCING
27
BL.
6
RNA
ISOLATION
AND
CDNA
SYNTHESIS
27
BL.
7
ISOLATION
OF
GENOMIC
DNA
FROM
MAMMALIAN
CELLS
28
BL.
8
SOUTHERN
BLOT
ANALYSIS
AND
DENSITOMETRY
29
BL
.9
POLYMERASE
CHAIN
REACTION
31
BL.
10
SYNTHESIS
OF
OLIGONUCLEOTIDES
31
B2
METHODS
OF
CELLULAR
BIOLOGY
33
B2.1
PREPARATION
OF
EMBRYONIC
FIBROBLASTS
33
B2.2
ES
CELL
CULTURE
34
B2.3
GENERATION
OF
THE
TARGETED
ES
CELL
LINES
36
B2.4
ISOLATION AND
FLOW
CYTOMETRIC
ANALYSIS
OF
CELLS
39
B2.5
IN
VITRO
CULTURE
OF
LYMPHOCYTES
42
B3.
GENERATION
OF
CHIMERIC
MICE
AND
ESTABLISHMENT
OF
42
MUTANT
MOUSE
STRAINS
B3.1
ANIMALS
42
B3.2
PRODUCTION
OF
CHIMERIC
MICE
AND
MUTANT
MOUSE
43
STRAINS
B4.
IMMUNIZATION
OF
MICE
46
B5.
ELISA
ANALYSIS
47
C.
RESULTS
50
CL.
GENERATION
AND
ANALYSIS
OF
C
K
T
MICE
50
CL.L
INACTIVATION
OF
THE
IGK
LOCUS
BY
HOMOLOGOUS
50
RECOMBINATION
CL.
2
MICE
HOMOZYGOUS
FOR
THE
C
K
T,
IE
K
T
AND
IEKL
MUTATION
53
LACK
K-BEARING
BUT
POSSESS
X-BEARING
B
CELLS
CL.
3
K-DEFICIENT
MICE
GENERATED
LESS
B
CELLS
THAN
CONTROL
57
MICE
BUT
CONTAIN
MANY
MORE
X-BEARING
CELLS
ALREADY
IN
THE
COMPARTMENT
OF
NEWLY
GENERATED
CELLS
CL.
4
THE
PRODUCTION
OF
X-BEARING
B
CELLS
DOES
NOT
REQUIRE
61
SIGNALS
RESULTING
FROM
V
X
-J
K
OR
RS
REARRANGEMENT
CL.
5
IN
NORMAL
MICE
MOST
NON-PRODUCTIVE,
BUT
VERY
FEW
65
PRODUCTIVE
V
X
-JX
JOINTS
ARE
CARRIED
BY
K
CHAIN
EXPRESSING
SPLENIC
B
CELLS
C2.
GENERATION
AND
ANALYSIS
OF
THE
C
K
R
MICE
68
C2.1
THE
SUBSTITUTION
OF
THE
HUMAN
C
K
FOR
THE
MOUSE
GENE
68
BY
HOMOLOGOUS
RECOMBINATION
C2.2
SEQUENCING
OF
THE
HUMAN
C
K
GENE
ISOLATED
FROM
THE
71
TARGETED
ES
CELLS
C2.3
MICE
CARRYING
THE
C
K
R
MUTATION
EXPRESS
CHIMERIC
71
IGK
CHAINS
C2.4
C
K
R
MUTANT
MICE
CAN
ELICIT NORMAL
ANTIBODY
RESPONSE
76
C2.5
SOMATIC
HYPERMUTATION
OCCURS
ON
THE
MUTANT
IGK
ALLELE
77
C3.
GENERATION
AND
ANALYSIS
OF
MICE
CARRYING
BOTH
HUMAN
C
K
79
AND
CYX
GENES
C3.1
THE
REPLACEMENT
OF
THE
MOUSE
CYX
GENE
WITH
THE
HUMAN
79
ONE
BY
HOMOLOGOUS
RECOMBINATION
C3.2
MICE
CARRYING
THE
CYXR
MUTATION
SYNTHESIZE
ANTIBODIES
91
CONTAINING
HUMANIZED
CHAINS
D.
DISCUSSION
93
DI.
GENE
TARGETING
AS
A
TOOL
TO
INTRODUCE
NEW
GENETIC
93
INFORMATION
INTO
THE
MAMMALIAN
GENOME
D2.
L
CHAIN
REARRANGEMENT
IN
NORMAL
AND
K
-DEFICIENT
MICE
95
D2.1
IGX
GENE
REARRANGEMENT
DOES
NOT
PROVIDE
POSITIVE
95
SIGNAL
FOR
THE
PRODUCTION
OF
X-PRODUCING
CELLS
D2.2
LAMBDA
GENE
REARRANGEMENTS
CAN OCCUR BEFORE
K
GENE
96
REARRANGEMENTS
IN
NORMAL
B
CELL
DEVELOPMENT
D2
.3
CO-ORDINATED
A
GENE
REARRANGEMENTS
ON
BOTH
CHROMOSOMAL
97
HOMOLOGUES
D2.4
ABUNDANT
PRODUCTION
OF
X-BEARING
CELLS
IN
THE
K
-
98
DEFICIENT
MICE
AND
A
SCENARIO
OF
L
CHAIN
ISOTYPE
CONTROL
D3.
B
CELL
DEVELOPMENT
IN
THE
C
K
R
MUTATION
MICE
101
D4
.
C
K
R
MUTANTS
EXHIBITE
NORMAL
PROFILE
OF
VARIOUS
IG
104
ISOTYPES
D5.
UTILITY
OF
THE
C
K
T
AND
'HUAB'
MUTANT
MICE
105
E.
SUMMARY
107
F.
REFERENCES
109 |
| any_adam_object | 1 |
| author | Zou, Yong-Rui |
| author_facet | Zou, Yong-Rui |
| author_role | aut |
| author_sort | Zou, Yong-Rui |
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| bvnumber | BV009631174 |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV009631174 |
| illustrated | Illustrated |
| indexdate | 2024-08-16T00:45:17Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006364462 |
| oclc_num | 634184408 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-11 |
| owner_facet | DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-11 |
| physical | 120 S. Ill., graph. Darst. |
| publishDate | 1993 |
| publishDateSearch | 1993 |
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| spelling | Zou, Yong-Rui Verfasser aut Gene targeting on the Ig loci analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies vorgelegt von Yong-Rui Zou 1993 120 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1994 B-Zelle (DE-588)4147096-5 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Genort (DE-588)4206912-9 gnd rswk-swf Immunglobuline (DE-588)4026626-6 gnd rswk-swf Gen-Targeting (DE-588)4294998-1 gnd rswk-swf Antikörper (DE-588)4002290-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Maus (DE-588)4169148-9 s B-Zelle (DE-588)4147096-5 s Antikörper (DE-588)4002290-0 s DE-604 Immunglobuline (DE-588)4026626-6 s Genort (DE-588)4206912-9 s Gen-Targeting (DE-588)4294998-1 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006364462&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zou, Yong-Rui Gene targeting on the Ig loci analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies B-Zelle (DE-588)4147096-5 gnd Maus (DE-588)4169148-9 gnd Genort (DE-588)4206912-9 gnd Immunglobuline (DE-588)4026626-6 gnd Gen-Targeting (DE-588)4294998-1 gnd Antikörper (DE-588)4002290-0 gnd |
| subject_GND | (DE-588)4147096-5 (DE-588)4169148-9 (DE-588)4206912-9 (DE-588)4026626-6 (DE-588)4294998-1 (DE-588)4002290-0 (DE-588)4113937-9 |
| title | Gene targeting on the Ig loci analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies |
| title_auth | Gene targeting on the Ig loci analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies |
| title_exact_search | Gene targeting on the Ig loci analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies |
| title_full | Gene targeting on the Ig loci analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies vorgelegt von Yong-Rui Zou |
| title_fullStr | Gene targeting on the Ig loci analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies vorgelegt von Yong-Rui Zou |
| title_full_unstemmed | Gene targeting on the Ig loci analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies vorgelegt von Yong-Rui Zou |
| title_short | Gene targeting on the Ig loci |
| title_sort | gene targeting on the ig loci analysis of b cell development in the k deficient mice and generation of the mouse strain producing humanized antibodies |
| title_sub | analysis of B cell development in the k-deficient mice and generation of the mouse strain producing humanized antibodies |
| topic | B-Zelle (DE-588)4147096-5 gnd Maus (DE-588)4169148-9 gnd Genort (DE-588)4206912-9 gnd Immunglobuline (DE-588)4026626-6 gnd Gen-Targeting (DE-588)4294998-1 gnd Antikörper (DE-588)4002290-0 gnd |
| topic_facet | B-Zelle Maus Genort Immunglobuline Gen-Targeting Antikörper Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006364462&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT zouyongrui genetargetingontheiglocianalysisofbcelldevelopmentinthekdeficientmiceandgenerationofthemousestrainproducinghumanizedantibodies |