HPLC: a practical user's guide
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York u.a.
VCH
1994
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 211 S. graph. Darst. |
| ISBN: | 1560816368 |
Internformat
MARC
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| 100 | 1 | |a McMaster, Marvin C. |e Verfasser |0 (DE-588)113962231 |4 aut | |
| 245 | 1 | 0 | |a HPLC |b a practical user's guide |c Marvin C. McMaster |
| 264 | 1 | |a New York u.a. |b VCH |c 1994 | |
| 300 | |a XII, 211 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a High performance liquid chromatography | |
| 650 | 0 | 7 | |a HPLC |0 (DE-588)4072641-1 |2 gnd |9 rswk-swf |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-006363753 | |
Datensatz im Suchindex
| _version_ | 1820595266289401856 |
|---|---|
| adam_text |
HPL
C
A PRACTICAL USER'S GUID
E
MARVIN C
. MCMASTER
PART I
: AN HPLC PRIMER
1
1.
WHY HPLC? ADVANTAGES AND DISADVANTAGES
3
1
.1 HOW DOES IT WORK?
4
1
.2 HOW ELSE COULD I GET MY SEPARATION? 1
0
2.
SELECTING AN HPLC SYSTEM 1
5
2.1 WHAT DO I LOOK FOR IN A SYSTEM? 1
5
2.2 FROM WHOM DOIBUYIT? 1
7
2
.3 WHAT WILL IT COST? 18
2.4 COLUMNS 20
3.
RUNNING YOUR CHROMATOGRAPH 2
5
3
.1 SETUP AND START-UP 2
5
3.2 SAMPLE PREPARATION AND COLUMN CALIBRATION 35
3.3 YOUR FIRST CHROMATOGRAM 3
7
PART II
: HPLC OPTIMIZATION 4
3
4.
SEPARATION MODELS 4
5
4
.1 PARTITION
4
5
4
.2 ION-EXCHANGE CHROMATOGRAPHY 56
4
.3 SIZE-EXCLUSION CHROMATOGRAPHY
57
4
.4 AFFINITY CHROMATOGRAPHY 5
9
5.
COLUMN PREPARATION 6
1
5.1 COLUMN VARIATIONS 6
1
5
.2 PACKING MATERIALS AND HARDWARE 6
4
5.3 COLUMN SELECTION 6
6
6.
COLUMN AGING, DIAGNOSIS, AND HEALING 7
1
6
.1 PACKING DEGRADATION-BONDED-PHASE LOSS 7
3
6
.2 DISSOLVING PACKING MATERIAL-END VOIDS 7
4
6.3 BOUND MATERIAL 7
6
6
.4 PRESSURE INCREASES 7
9
6.5 COLUMN CHANNELING-CENTER VOIDS 8
0
6
.6 NORMAL PHASE, ION EXCHANGE, AND SIZE COLUMNS 82
7.
MODIFICATIONS OF PARTITION CHROMATOGRAPHY 8
5
7
.1 REVERSE PHASE 85
7.2 ACIDIC-PHASE SILICA 89
7
.3 PARTITION MODE SELECTION
8
9
7
.4 HYDROPHILIC SEPARATIONS 90
8.
"NONPARTITION" CHROMATOGRAPHY 9
3
8.1 ION EXCHANGE 93
8.2 SIZE EXCLUSION
9
5
8
.3 AFFINITY CHROMATOGRAPHY 9
7
9.
HARDWARE SPECIFICS 9
9
9.1 SYSTEM PROTECTION 9
9
9.2 PUMPING 10
2
9.3 INJECTORS AND AUTOSAMPLERS
10
9
9
.4 DETECTORS
10
9
9
.5 FRACTION COLLECTORS
11
5
9
.6 DATA COLLECTION AND PROCESSING 11
6
10.
TROUBLESHOOTING AND OPTIMIZATION 11
7
10
.1 HARDWARE AND TOOLS-SYSTEM PACIFICATION 11
7
10
.2 REVERSE ORDER DIAGNOSIS
12
1
10
.3 INTRODUCTION TO DATA ACQUISITION
123
PART
III
:
HPLC UTILIZATION 12
7
11.
PREPARATIVE CHROMATOGRAPHY 12
9
11
.1 ANALYTICAL PREPARATIVE
130
11
.2 SEMIPREPARATIVE
13
1
11
.3 "TRUE"
PREPARATIVE
13
1
12.
SAMPLE PREPARATION AND METHODS DEVELOPMENT 13
5
12
.1 SAMPLE PREPARATION
135
12
.2 METHODS DEVELOPMENT
143
12
.3 GRADIENT DEVELOPMENT
148
13.
APPLICATION LOGIC
: SEPARATIONS OVERVIEW 15
1
13
.1 FAT-SOLUBLE VITAMINS, STEROIDS,
AND
LIPIDS
15
1
13
.2 WATER-SOLUBLE VITAMINS,
CARBOHYDRATES, AND
ACIDS
152
13
.3 DNA FAMILY: NUCLEIC ACIDS,
NUCLEOSIDES/NUCLEOTIDES,
AND RNA
/
DNA 152
13.4 PROTEIN FAMILY: AMINO ACIDS, PEPTIDES,
AND
PROTEINS
154
13
.5 CLINICAL DRUG MONITORING, TOXICOLOGY,
AND
FORENSICS
155
13
.6
ENVIRONMENTAL AND
REACTION
MONITORING
15
5
13
.7
APPLICATION
TRENDS
15
6
14.
AUTOMATION
: SEPARATIONS AND DATA ACQUISITION 159
14
.1 ANALOG-TO-DIGITAL INTERFACING
15
9
14
.2 DIGITAL
INFORMATION EXCHANGE
16
1
14
.3
HPLC
SYSTEM CONTROL
AND AUTOMATION
16
1
14
.4 DATA COLLECTION AND INTERPRETATION
16
2
14
.5
AUTOMATED
METHODS
DEVELOPMENT
16
4
14.6 DATA
EXPORTATION
TO THE REAL WORLD
16
8
15.
WHERE DO WE GO FROM HERE? 17
1
15
.1 MICROSYSTEMS
AND
SUPERDETECTORS
17
1
15
.2 LC/MS PRIMER 17
2
15.3 NEW COLUMN DIRECTIONS
18
1
15
.4 DEDICATED SYSTEMS
:
PUSHBUTTON
CLINICAL
AND
THE
PERSONAL HPL
C
18
2
APPENDIXES 18
3
A.
PERSONAL SEPARATION
GUIDE
18
5
B.
A
GLOSSARY
OF HPLC TERMS 187
C.
HPLC TROUBLESHOOTING QUICK REFERENCE
193
D.
LABORATORY EXPERIMENTS 19
7
LABORATORY 1-SYSTEM START-UP AND COLUMN QC 19
7
LABORATORY 2-SAMPLE PREPARATION AND METHOD DEVELOPMEN
T
199
LABORATORY 3-COLUMN AND SOLVENT SWITCHING AND PACIFICATIO
N
200
E.
SELECTED REFERENCE LIST 20
3
INDEX 205 |
| any_adam_object | 1 |
| author | McMaster, Marvin C. |
| author_GND | (DE-588)113962231 |
| author_facet | McMaster, Marvin C. |
| author_role | aut |
| author_sort | McMaster, Marvin C. |
| author_variant | m c m mc mcm |
| building | Verbundindex |
| bvnumber | BV009629004 |
| callnumber-first | Q - Science |
| callnumber-label | QD79 |
| callnumber-raw | QD79.C454 |
| callnumber-search | QD79.C454 |
| callnumber-sort | QD 279 C454 |
| callnumber-subject | QD - Chemistry |
| classification_rvk | VG 7500 WC 3460 |
| classification_tum | CHE 232f |
| ctrlnum | (OCoLC)29429519 (DE-599)BVBBV009629004 |
| dewey-full | 543/.0894 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 543 - Analytical chemistry |
| dewey-raw | 543/.0894 |
| dewey-search | 543/.0894 |
| dewey-sort | 3543 3894 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Biologie Chemie |
| format | Book |
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| id | DE-604.BV009629004 |
| illustrated | Illustrated |
| indexdate | 2025-01-07T13:02:25Z |
| institution | BVB |
| isbn | 1560816368 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006363753 |
| oclc_num | 29429519 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-29T DE-19 DE-BY-UBM DE-634 DE-83 |
| owner_facet | DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-29T DE-19 DE-BY-UBM DE-634 DE-83 |
| physical | XII, 211 S. graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| publisher | VCH |
| record_format | marc |
| spelling | McMaster, Marvin C. Verfasser (DE-588)113962231 aut HPLC a practical user's guide Marvin C. McMaster New York u.a. VCH 1994 XII, 211 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier High performance liquid chromatography HPLC (DE-588)4072641-1 gnd rswk-swf HPLC (DE-588)4072641-1 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006363753&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | McMaster, Marvin C. HPLC a practical user's guide High performance liquid chromatography HPLC (DE-588)4072641-1 gnd |
| subject_GND | (DE-588)4072641-1 |
| title | HPLC a practical user's guide |
| title_auth | HPLC a practical user's guide |
| title_exact_search | HPLC a practical user's guide |
| title_full | HPLC a practical user's guide Marvin C. McMaster |
| title_fullStr | HPLC a practical user's guide Marvin C. McMaster |
| title_full_unstemmed | HPLC a practical user's guide Marvin C. McMaster |
| title_short | HPLC |
| title_sort | hplc a practical user s guide |
| title_sub | a practical user's guide |
| topic | High performance liquid chromatography HPLC (DE-588)4072641-1 gnd |
| topic_facet | High performance liquid chromatography HPLC |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006363753&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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