Gel electrophoresis of proteins and nucleic acids: selected techniques
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
de Gruyter
1994
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | XV, 352 S. Ill., graph. Darst. |
| ISBN: | 3110138964 |
Internformat
MARC
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| 084 | |a CHE 228f |2 stub | ||
| 100 | 1 | |a Allen, Robert C. |d 1930- |e Verfasser |0 (DE-588)130033898 |4 aut | |
| 245 | 1 | 0 | |a Gel electrophoresis of proteins and nucleic acids |b selected techniques |c Robert C. Allen ; Bruce Budowle |
| 264 | 1 | |a Berlin [u.a.] |b de Gruyter |c 1994 | |
| 300 | |a XV, 352 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Literaturangaben | ||
| 650 | 7 | |a Eletroforese |2 larpcal | |
| 650 | 7 | |a Química |2 larpcal | |
| 650 | 7 | |a Ácidos nucléicos |2 larpcal | |
| 650 | 4 | |a Electrophoresis, Polyacrylamide Gel |x methods | |
| 650 | 4 | |a Gel electrophoresis |x Methodology | |
| 650 | 4 | |a Nucleic Acids |x analysis | |
| 650 | 4 | |a Nucleic acids |x Analysis | |
| 650 | 4 | |a Proteins |x Analysis | |
| 650 | 4 | |a Proteins |x analysis | |
| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Isoelektrische Fokussierung |0 (DE-588)4162498-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Nucleinsäuren |0 (DE-588)4172117-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zweidimensionale Elektrophorese |0 (DE-588)4221393-9 |2 gnd |9 rswk-swf |
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| 689 | 3 | 0 | |a Zweidimensionale Elektrophorese |0 (DE-588)4221393-9 |D s |
| 689 | 3 | |5 DE-604 | |
| 700 | 1 | |a Budowle, Bruce |e Verfasser |4 aut | |
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| adam_text |
CONTENTS
CHAPTER
1
SUPPORT
MEDIA
1
POLYACRYLAMIDE
.
1
1.1
MONOMER
.
3
1.2
CROSSLINKERS
.
4
1.3
CATALYSTS
.
4
1.4
POLYMERIZATION
BY-PRODUCTS
.
6
1.5
EMPTY
GELS
.
6
1.5.1
REHYDRATABLE
GELS
.
7
1.5.2
GEOMETRIE
STABILITY
.
7
1.6
POLYMERIZATION
CONDITIONS
.
8
1.6.1
OXY
GEN
.
9
1.6.2
MATERIAL
EFFECTS
.
9
1.7
PORE
SIZE:
RELATIONSHIP
OF
MONOMER
AND
CROSSLINKER
.
10
1.7.1
INTERACTION
BETWEEN
MONOMER
AND
CROSSLINKER
.
12
1.7.2
INTERFACE
EFFECTS
ON
PORE
SIZE
.
13
1.8
OTHER
CROSSLINKING
AGENTS
.
14
1.9
OTHER
POLYMERS
.
15
2
AGAROSE
.
16
2.1
STRUCTURE
.
16
2.2
AGAROSE
FOR
PROTEIN
SEPARATION
.
17
2.2.1
ISOELECTRIC
FOCUSING
.
18
2.2.2
AGAROSES
WITH
MOLECULAR
SIEVING
CHARACTERISTICS
.
19
2.2.3
SIEVING
AGAROSES
FOR
NUCLEIC
ACID
SEPARATIONS
.
20
REFERENCES
.
22
CHAPTER
2
GEL
ELECTROPHORESIS
OF
PROTEINS
AND
NUCLEIC
ACIDS
1
BACKGROUND
.
26
2
PRINCIPLES
OF
ELECTROPHORESIS
.
26
2.1
CHARGE
.
27
2.2
MOBILITY
.
27
2.3
VELOCITY
.
28
2.4
LONIC
STRENGTH
.
28
2.5
OHMIC
HEATING
.
29
3
CONTROL
OF
JOULE
HEAT
.
29
3.1
POWER
APPLICATION
AND
ELECTROCHEMISTRY
.
29
X
CONTENTS
3.2
EQUIPMENT-BASED
CONTROL
OF
HEAT
.
30
3.2.2
PELTIER
COOLING
.
31
4
CONTINUOUS
ZONE
(CZE)
VS.
MULTIPHASIC
(MZE)
ELECTROPHORESIS
.
32
4.1
STARTING
ZONE
WIDTH
.32
4.2
ZONE
SHARPENING
METHODS
.
33
4.3
GEL
TYPE
ROD
OR
FLAT
SLAB
GELS
.
34
4.3.1
SLAB
GELS
.
34
5
MOVING
BOUNDARY
BUFFER
SYSTEMS
FOR
PRODUCING
NARROW
STARTING
ZONES
.
35
5.1
OMSTEIN-DAVIS
TECHNIQUE:
DISCONTINUOUS
BUFFERS
AND
PH
.
36
5.2
DISCONTINUOUS
BUFFERS
AT
AN
INITIAL
CONSTANT
PH
.
38
5.3
ALTERNATIVE
TECHNIQUES
.
41
5.4
APPLICATION
OF
MZE
GEL
ELECTROPHORESIS
.
43
5.4.1
CONDUCTIVITY
SHIFT
.43
5.4.2
UNSTACKING
.
44
5.4.3
TRAILING
ION
EFFECTS
.
45
5.5
RESOLUTION
OF
PROTEINS
BY
NET
CHARGE
.
46
6
CZE
ELECTROPHORESIS
.
47
6.1
BACKGROUND
.
47
6.2
COMPARATIVE
TECHNIQUES
.
48
6.2.1
CONDUCTIVITY
SHIFT
.
48
6.2.2
PORE
GRADIENTS
.
49
6.3
SUMMATION
OF
CZE
VS.
MZE
BUFFER
SYSTEMS.
50
7
MOLECULAR
WEIGHT
DETERMINATION
.
52
7.1
NATIVE
PROTEINS
.
52
7.1.1
FERGUSON
PLOTS
.52
7.2
DENATURING
SYSTEMS
FOR
PROTEINS
.
56
7.2.1
PORE
GRADIENT
SDS
ELECTROPHORESIS
.
58
7.3
VERTICAL
VS
HORIZONTAL
GELS
.
61
8
NUCLEIC
ACID
SEPARATION
.
62
8.1
BACKGROUND
.
62
8.2
OBSERVATIONSANDMODELS
.62
8.2.1
EFFECTS
OF
DNA
CONFORMATION
ON
ELECTROPHORESIS
.
63
8.3
THEORETICAL
MODELS
.64
8.4
BUFFER
SELECTION
.
66
8.4.1
CONTINUOUS
BUFFER
SYSTEMS
.
66
8.4.2
DISCONTINUOUS
BUFFERS
.
68
8.4.3
MODIFIER
EFFECTS
ON
RESOLUTION
.
69
8.4.4
R
F
AS
AN
IDENTIFIER
.
69
8.5
SEQUENCING
WITH
DISCONTINUOUS
BUFFERS
.
71
8.6
GEL
CHARACTERISTICS
AND
ELECTROPHORESIS
CONDITIONS
.
74
8.7
THE
POLYMERASE
CHAIN
REACTION
(PCR)
AMPLIFIED
PRODUCTS
.
77
REFERENCES
.79
CONTENTS
XI
CHAPTER
3
ISOELECTRIC
FOCUSING
1
INTRODUCTION
.
87
2
BACKGROUND
.
87
2.1
FACTORS
AFFECTING
RESOLUTION
IN
ISOELECTRIC
FOCUSING
.
90
2.1.1
LENGTH
OF
THE
GEL
.
90
2.1.2
RESOLVING
POWER
.
91
2.1.3
VOLTAGE
GRADIENT
EFFECT
.
91
2.1.4
PEAK
CAPACITY
.
92
3
ISOELECTRIC
FOCUSING
PROCEDURES
.
93
3.1
CONVENTIONAL
CARRIER
AMPHOLYTES
.
93
3.2
SYNTHETIC
CARRIER
AMPHOLYTE
(SCAM)
APPROACHES
.
95
3.2.1
PRECAUTIONS
.
96
3.3
HYBRID
ISOELECTRIC
FOCUSING
.
97
3.4
SIMPLE
BUFFER
FOCUSING
.
98
3.5
PRACTICAL
CONSIDERATIONS
.99
4
PRACTICAL
APPLICATION
OF
THEORY
.
100
4.1
HEAT
TRANSFER
AND
THERMAL
ZONE
SPREADING
.
100
4.2
VOLT/HOURS,
VOLTAGE
GRADIENT
AND
RESOLUTION
.
103
4.3
INCREASING
GEL
LENGTH
.
104
4.4
POOR
RESOLUTION
OR
THE
"
WAVY
BAND
"
PHENOMENON
.
104
4.5
ADDITIVES
.
105
4.5.1
FOCUSING
IN
THE
PRESENCE
OF
DETERGENTS
.
105
4.5.2
FOCUSING
WITH
UREA
.
107
5
PAGIF
IN
CLINICAL
MEDICINE
.
110
5.1
STANDARDIZATION
OF
SAMPLE
COLLECTION
AND
HANDLING
.
HO
5.2
STANDARDIZATION
OF
APPARATUS
AND
OPERATION
.
110
5.3
PLASMA
PROTEINS
.
111
5.3.1
IMMUNOGLOBULINS
.
111
5.3.2
BETA
GLOBULINS
.
113
5.3.3
ALPHA
2-GLOBULINS
.
115
5.3.4
ALPHA
1
-GLOBULINS
.
115
5.3.5
ALPHA-1
-LIPOPROTEINS
.
116
5.4
HEMOGLOBIN
.
117
5.4.1
THALASSEMIA
DETECTION
.
117
5.5
BODY
FLUIDS
.
120
5.5.1
CEREBROSPINAL
FLUID
.
120
5.5.2
URINE
.
121
5.5.3
SALIVARY
PROTEINS
.
122
5.6
ENZYMES
.
123
5.6.1
PLASMA
ENZYMES
.
123
5.7
TISSUE
PROTEINS
AND
ENZYMES
.
124
XII
CONTENTS
5.8
AMNIOTIC
FLUID
.
126
5.9
GENETIC
AND
FORENSIC
APPLICATION
.
128
5.10
AGRICULTURAL
APPLICATIONS
.
131
5.11
BIOCHEMICAL
APPLICATIONS
.
133
5.11.1
TITRATION
CURVES
.
133
5.11.2
AFFMITY
TITRATIONS
.
135
5.12
PREPARATIVE
ISOELECTRIC
FOCUSING
.
136
5.13
GRADIENT
CONTROL
.
137
5.13.1
GRADIENT
BROADENING
.
138
5.13.
2
GRADIENT
FLATTENING
.
139
6
TROUBLE
SHOOTING
GUIDE
.
140
REFERENCES
.
143
CHAPTER
4
TWO-DIMENSIONAL
ELECTROPHORESIS
1
INTRODUCTION
.
157
1.1
BACKGROUND
.
157
1.2
SAMPLE
TREATMENT
.
158
1.2.1
SOLUBILIZATION
OF
THE
SAMPLE
FOR
PAGIF
.
159
1.3
THEORETICAL
CONSIDERATIONS
AFFECTING
RESOLVING
POWER
.
164
1.3.1
ISOELECTRIC
FOCUSING
IN
ROD
GELS
.
165
1.3.2
ISOELECTRIC
FOCUSING
IN
FLAT
SLABS
IN
THE
FIRST
DIMENSION
.
166
1.3.3
EQUILIBRATION,
APPLICATION
AND
PROTEIN
TRANSFER
.
166
1.3.4
CONTACT
BETWEEN
THE
FIRST
AND
SECOND
DIMENSION
GELS
.
168
1.3.5
THE
SECOND
DIMENSION
SDS-PAGE
.
169
1.3.6
SECOND
DIMENSION
CHARACTERISTICS
.
170
1.3.7
FIXATION
AND
STAINING
.
171
1.3.8
REPRODUCIBILITY
.
172
1.4
OTHER
MULTIPARAMETER
TECHNIQUES
.
174
1.4.1
BLOTTING
AND
RNICRO
SEQUENCING
.
174
1.4.2
DOUBLE
ONE-DIMENSIONAL
ELECTROPHORESIS
.
176
1.4.3
IMMUNODELETION
FOLLOWED
BY
2-D
.
177
1.5
PSEUDO-LIGAND
AFFINITY
CHROMATOGRAPHY
AND
ISOELECTRIC
FOCUSING
.
178
1.6
PSEUDO-LIGAND
AFFINITY
CHROMATOGRAPHY
FOLLOWED
BY
2-D
.
180
1.7
TWO-DIMENSIONAL
ELECTROPHORESIS
AS
A
CLINICAL
TOOL
.
183
2
TWO-DIMENSIONAL
DATABASES
.
187
2.1
TRANSFORMED
CELLS
.
188
2.2
ESCHERICHIA
COLI
DATABASE
.
189
2.3
HUMAN
FIBROBLASTS
.
190
2.4
GERMINAL
AND
DEVELOPMENTAL
CELLS
.
190
2.5
PLASMA
PROTEINS
.
191
CONTENTS
XIII
2.6
RED
BLOOD
CELL
PROTEINS
.
191
2.7
LYMPHOCYTES
.
191
2.8
DISEASE
RELATED
DATABASES
.
192
2.8.1
MELANOMA
.
192
2.8.2
COLON
CANCER
.
192
2.8.3
MYOCARDIAL
DISEASE
.
193
2.8.4
CONNECTIVETISSUEDISEASE
.
193
REFERENCES
.
194
CHAPTER
5
COMPONENT
VISUALIZATION
1
INTRODUCTION
.
204
2
PROTEIN
STAINS
.
204
2.1.
COOMASSIE
BRILLIANT
BLUE
.
205
2.2
GLYCOPROTEIN
STAINS
.
206
2.2.1
PAS
STAIN
.
206
2.2.2
SPECIFIC
LECTIN
PROBES
.
207
2.3
CROWLE
'
S
DOUBLE
STAIN
.
210
2.4
LIPOPROTEIN
PRESTAIN
.
210
2.5
HEME-BINDING
PROTEINS
.
211
2.6
NEGATIVE
GOLD
STAIN
.
212
3
SILVER
STAINING
.
213
3.1
BACKGROUND
.
213
3.2
STEPS
IN
THE
VARIOUS
SILVER
STAIN
PROCEDURES
FOR
PROTEINS
.
214
3.2.1
FIXATION
OF
PROTEINS
FOLLOWING
ELECTROPHORESIS
.
214
3.2.2
DIAMMINE
SILVER
STAINS
.
215
3.2.3
NON-DIAMMINE
SILVER
STAINING
.
YY.
.
216
3.2.4
DEVELOPMENT
OF
SILVER
BY
REDUCTION
TO
ITS
METALLIC
FORM
.
217
3.2.5
RECYCLING
SILVER
STAINING
.
218
3.2.6
REACTION
TEMPERATURES
.
219
3.3
LIPOPROTEINS
.
219
3.4
SILVER
STAINING
PROTEINS
IN
AGAROSE
.
219
3.5
SILVER
STAINING
PROCEDURES
IN
ACRYLAMIDE
.
220
4
ENZYME
VISUALIZATION
.
224
4.1
HYDROLASES
.
224
4.2
OXIREDUCTASES
.
228
4.3
OXIDASES
.
228
4.4
DEHYDROGENASES
.
228
4.5
TRANSFERASES,
LYASES,
ISOMERASES
AND
LIGASES
.
231
4.6
TRANSFER
METHODS
.
234
4.6.1
REPLICATE
PRINT
TECHNIQUE
.
234
XIV
CONTENTS
4.6.2
NITROCELLULOSE
ENZYME
BLOT
TECHNIQUE
.
234
4.6.3
IMMUNOPRINTING
.
235
4.6.4
PRINTS
WITH
FLUORESCEIN
AND
RHODAMINE
DYES
.
238
4.6.5
ENHANCEMENT
TECHNIQUES
.
238
4.6.6
CHEMILUMINESCENCE
.
239
4.7
ELECTROBLOTTING
PROTEINS
.
241
4.7.1
PEROXIDASE-CONJUGATED
ANTIBODY
.
242
4.7.2
CHEMILUMINESCENCE
.
246
4.8
OPERATING
HINTS
.
248
4.8.1
CAUSES
OF
PROTEIN
LOSS
.
249
4.8.2
BACKGROUND
STAINING
.
250
4.8.3
ANTIBODY
ASSOCIATED
PROBLEMS
.
251
4.9
AUTORADIOGRAPHY
.
251
4.10
FLUOROGRAPHY
.
252
4.11
DUAL
LABELING
.
253
5
NUCLEIC
ACID
VISUALIZATION
.
254
5.1
SINGLE-STRANDED
IN
DNA
SEQUENCING
.
254
5.1.1
FLUORESCENT
VISUALIZATION
OF
SEQUENCE
GELS
.
254
5.1.2
CHEMILUMINESCENT
VISUALIZATION
OF
SEQUENCING
GELS
.
255
5.2
DOUBLE-STRANDED
DNA
RFLP
AND
PCR
AMPLIFIED
PRODUCTS
.
258
5.2.1
ETHIDIUM
BROMIDE
STAINING
OF
DOUBLE-STRANDED
DNA
.
258
5.2.2
HOECHST
33258
DYE
.
259
5.2.3
RADIOACTIVE
LABELS
.
259
5.3
PHOTOSTIMULABLE
STORAGE
PHOSPHORS
FOR
AUTORADIOGRAPHIC
DETECTION
.
259
5.4
PCR
PRODUCTS
.
262
5.4.1
SILVER
STAINING
.
262
5.4.2
CHEMILUMINESCENT
VISUALIZATION
OF
PCR
PRODUCTS
AND
PROBES
.
264
5.4.3
DIRECT
FLUORESCENT
PCR
LABELING
AND
DETECTION
.
265
REFERENCES
.
266
CHAPTER
6
IMAGE
HANDLING
PROCEDURES
1
PHOTOGRAPHY
.
273
2
DENSITOMETRY
AND
DIRECT
IMAGE
PROCESSING
.
276
2.1
IMAGE
TRANSDUCERS
.
277
2.1.1
HAND
SCANNERS
.
277
2.1.2
FLATBED
SCANNERS
.
278
2.1.3
SOLID
STATE
CCD
CAMERA
BASED
SCANNERS
.
279
2.1.4
DIGITIZING
TABLET
INPUT
.
280
2.1.5
LASER
DENSITOMETRY
INCLUDING
PHOSPHOR
IMAGERS
.
280
2.1.6
SIMULTANEOUS
SEPARATION
AND
SCANNING
INSTRUMENTATION
.
282
2.1.7
MICROSCOPE
SCANNERS.
282
CONTENTS
XV
REFERENCES
.
284
CHAPTER
7
PROTOCOLS
AND
PROCEDURES
1
GEL
CASTING
.
285
1.1
PURIFICATION
OF
ACRYLAMIDE
.
285
1.2
STOCK
SOLUTIONS
.
286
1.2.1
ACRYLAMIDE
.
286
1.3
CASTING
PROCEDURES
.
287
1.3.1
OMSTEIN-DAVIS
SYSTEM
.
288
1.3.2
ALLEN-MOORE
SYSTEM
.
289
1.3.3
DENATURING
STEP-GRADIENT
VERTICAL
GEL
.
290
1.3.4
ULTRATHIN-LAYER
GELS
FLAP
TECHNIQUE
.
291
1.3.5
CAPILLARY
CAST
ULTRATHIN-LAYER
GELS
.
292
1.3.6
ULTRATHIN-LAYER
OPEN
FACED
GRADIENT
GELS
FOR
SDS-PAGE
.
293
1.3.7
SEQUENCING
GELS
BACKED
ON
FILM
.
295
2
AGAROSE
.
296
2.1
CASTING
AGAROSE
GELS
FOR
ISOELECTRIC
FOCUSING
.
296
2.2
SEPARATION
OF
DOUBLE-STRANDED
DNA
FRAGMENTS
ON
AGAROSE
.
303
2.3
GRANULAT
GEL-BED
FOR
PREPARATIVE
ISOELECTRIC
FOCUSING
.
305
3
DNAPROBES
.
308
3.1
RADIOLABELING
OF
DNA
PROBES
.
308
3.2
DNA
EXTRACTION
PROCEDURES
.
312
3.2.1
ORGANIC
EXTRACTION
OF
DNAFROM
WHOLE
BLOOD
.
312
3.2.2
EXTRACTION
OF
DNA
FROM
FLUID
STAINED
MATERIAL
.
314
3.2.3
INORGANIC
EXTRACTION
OF
DNA
FROM
WHOLE
BLOOD
.
315
3.2.4
RESTRICTION
DIGESTION
OF
NON-ORGANIC
EXTRACTED
DNA
.
317
3.2.5
DIFFERENTIAL
EXTRACTION
OF
DNA
FROM
SPERM
CONTAINING
STAINS
.
318
3.2.6
CHELEX
EXTRACTION
OF
DNA
FROM
WHOLE
BLOOD
OR
STAINS
.
320
3.2.7
CHELEX
EXTRACTION
OF
DNA
FROM
SPERM-CONTAINING
STAINS
.
321
3.2.8
EXTRACTION
OF
DNA
FROM
BONE
.
322
3.3
QUANTIFICATION
OF
HUMAN
GENOMIC
DNA
.
324
3.3.1
QUANTIFICATION
OF
DNA
FOR
PCR
.
325
3.4
RFLP
HYBRIDIZATION
ASSAY
CONDITIONS
.
327
3.4.1
RFLP
ASSAY
CONDITIONS
.
328
3.5
ELECTROPHORETIC
SEPARATION
OF
PCR-AMPLIFIED
VNTR
PRODUCTS
.
328
3.5.2
SIZE
DETERMINATION
OF
PCR-AMPLIFIED
PRODUCTS
.
331
REFERENCES
.
332
ABBREVIATIONS
.
335
SUBJECT
INDEX
.
339 |
| any_adam_object | 1 |
| author | Allen, Robert C. 1930- Budowle, Bruce |
| author_GND | (DE-588)130033898 |
| author_facet | Allen, Robert C. 1930- Budowle, Bruce |
| author_role | aut aut |
| author_sort | Allen, Robert C. 1930- |
| author_variant | r c a rc rca b b bb |
| building | Verbundindex |
| bvnumber | BV009563472 |
| callnumber-first | Q - Science |
| callnumber-label | QP519 |
| callnumber-raw | QP519.9.G42 |
| callnumber-search | QP519.9.G42 |
| callnumber-sort | QP 3519.9 G42 |
| callnumber-subject | QP - Physiology |
| classification_rvk | VG 8400 WC 3440 |
| classification_tum | CHE 228f |
| ctrlnum | (OCoLC)844298856 (DE-599)BVBBV009563472 |
| dewey-full | 574.19/245 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 574 - [Unassigned] |
| dewey-raw | 574.19/245 |
| dewey-search | 574.19/245 |
| dewey-sort | 3574.19 3245 |
| dewey-tens | 570 - Biology |
| discipline | Chemie / Pharmazie Biologie Chemie |
| format | Book |
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| id | DE-604.BV009563472 |
| illustrated | Illustrated |
| indexdate | 2024-08-20T00:52:59Z |
| institution | BVB |
| isbn | 3110138964 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006319963 |
| oclc_num | 844298856 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-703 DE-19 DE-BY-UBM DE-20 DE-91G DE-BY-TUM DE-11 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-703 DE-19 DE-BY-UBM DE-20 DE-91G DE-BY-TUM DE-11 DE-188 |
| physical | XV, 352 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| publisher | de Gruyter |
| record_format | marc |
| spelling | Allen, Robert C. 1930- Verfasser (DE-588)130033898 aut Gel electrophoresis of proteins and nucleic acids selected techniques Robert C. Allen ; Bruce Budowle Berlin [u.a.] de Gruyter 1994 XV, 352 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturangaben Eletroforese larpcal Química larpcal Ácidos nucléicos larpcal Electrophoresis, Polyacrylamide Gel methods Gel electrophoresis Methodology Nucleic Acids analysis Nucleic acids Analysis Proteins Analysis Proteins analysis Proteine (DE-588)4076388-2 gnd rswk-swf Isoelektrische Fokussierung (DE-588)4162498-1 gnd rswk-swf Nucleinsäuren (DE-588)4172117-2 gnd rswk-swf Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd rswk-swf Gelelektrophorese (DE-588)4123267-7 gnd rswk-swf Nucleinsäuren (DE-588)4172117-2 s Gelelektrophorese (DE-588)4123267-7 s DE-604 Proteine (DE-588)4076388-2 s Isoelektrische Fokussierung (DE-588)4162498-1 s Zweidimensionale Elektrophorese (DE-588)4221393-9 s Budowle, Bruce Verfasser aut DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006319963&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Allen, Robert C. 1930- Budowle, Bruce Gel electrophoresis of proteins and nucleic acids selected techniques Eletroforese larpcal Química larpcal Ácidos nucléicos larpcal Electrophoresis, Polyacrylamide Gel methods Gel electrophoresis Methodology Nucleic Acids analysis Nucleic acids Analysis Proteins Analysis Proteins analysis Proteine (DE-588)4076388-2 gnd Isoelektrische Fokussierung (DE-588)4162498-1 gnd Nucleinsäuren (DE-588)4172117-2 gnd Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd Gelelektrophorese (DE-588)4123267-7 gnd |
| subject_GND | (DE-588)4076388-2 (DE-588)4162498-1 (DE-588)4172117-2 (DE-588)4221393-9 (DE-588)4123267-7 |
| title | Gel electrophoresis of proteins and nucleic acids selected techniques |
| title_auth | Gel electrophoresis of proteins and nucleic acids selected techniques |
| title_exact_search | Gel electrophoresis of proteins and nucleic acids selected techniques |
| title_full | Gel electrophoresis of proteins and nucleic acids selected techniques Robert C. Allen ; Bruce Budowle |
| title_fullStr | Gel electrophoresis of proteins and nucleic acids selected techniques Robert C. Allen ; Bruce Budowle |
| title_full_unstemmed | Gel electrophoresis of proteins and nucleic acids selected techniques Robert C. Allen ; Bruce Budowle |
| title_short | Gel electrophoresis of proteins and nucleic acids |
| title_sort | gel electrophoresis of proteins and nucleic acids selected techniques |
| title_sub | selected techniques |
| topic | Eletroforese larpcal Química larpcal Ácidos nucléicos larpcal Electrophoresis, Polyacrylamide Gel methods Gel electrophoresis Methodology Nucleic Acids analysis Nucleic acids Analysis Proteins Analysis Proteins analysis Proteine (DE-588)4076388-2 gnd Isoelektrische Fokussierung (DE-588)4162498-1 gnd Nucleinsäuren (DE-588)4172117-2 gnd Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd Gelelektrophorese (DE-588)4123267-7 gnd |
| topic_facet | Eletroforese Química Ácidos nucléicos Electrophoresis, Polyacrylamide Gel methods Gel electrophoresis Methodology Nucleic Acids analysis Nucleic acids Analysis Proteins Analysis Proteins analysis Proteine Isoelektrische Fokussierung Nucleinsäuren Zweidimensionale Elektrophorese Gelelektrophorese |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006319963&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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