Studies on the physiological role of the calcium-dependent protease, calpain:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
1994
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 104 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV009526148 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 940329s1994 gw ad|| m||| 00||| eng d | ||
| 016 | 7 | |a 940537044 |2 DE-101 | |
| 035 | |a (OCoLC)59821027 | ||
| 035 | |a (DE-599)BVBBV009526148 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-91 |a DE-11 |a DE-188 | ||
| 100 | 1 | |a Miller, Anita C. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Studies on the physiological role of the calcium-dependent protease, calpain |c von Anita C. Miller |
| 264 | 1 | |c 1994 | |
| 300 | |a XVII, 104 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Karlsruhe, Univ., Diss., 1994 | ||
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006289947&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-006289947 | |
Datensatz im Suchindex
| _version_ | 1812454009530744832 |
|---|---|
| adam_text |
TABLE OF CONTENTS
ACKNOWLEDGMENTS.
ABBREVIATIONS.
TABLE OF CONTENTS.
LIST OF FIGURES.
LIST OF TABLES.
CHAPTER
I: INTRODUCTION.
CHAPTER
II:
GENERAL BACKGROUND.
THE PROTEASE SYSTEM.
CYSTEINE PROTEINASES.
PAPAIN.
CATHEPSIN .
THE CALPAIN SYSTEM.
HISTORICAL BACKGROUND.
CALPAIN GENE AND PROTEIN STRUCTURE.
CALPAIN ACTIVATION PROCESS.
SUBSTRATE SPECIFICITY.
PEPTIDE SUBSTRATES.
PROTEIN SUBSTRATES.
CALPAIN INHIBITORS.
CALPASTATIN.
OTHER INHIBITORY PROTEINS.
SYNTHETIC INHIBITORS.
CALPAIN ACTIVATORS.
CALCIUM.
PROTEIN KINASE C (PKC).
CHAPTER
III:
MATERIALS AND METHODS.
MATERIALS.
CHEMICALS.
ANIMALS.
CELL CULTURE.
METHODS.
.VI
,.VII
.XI
.XV
.XVII
.1
.3
.3
.5
.5
.5
.5
.5
.5
.8
.10
.10
.10
.11
.11
.13
.13
.14
.14
.16
.19
.19
.19
.19
.19
.21
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/940537044
TABLE OF CONTENTS
XII
PREPARATION OF EXTRACTS FOR ENZYMATIC ACTIVITY AND IMMUNOBLOTTING
ANALYSES.
STUDIES USING LUNG TISSUE.
STUDIES USING LUNG EPITHELIAL CELL LINES.
STUDIES USING THYMOCYTES.
STUDIES USING MACROPHAGES.
ENZYME ANALYSES.
PROTEIN CONCENTRATION.
CALPAIN ACTIVITY ASSAY.
PROTEIN SEPARATION TECHNIQUES.
SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE).
WESTERN IMMUNOBLOT ANALYSIS.
PKC.
CALPAIN.
FAST PROTEIN LIQUID CHROMATOGRAPHY (FPLC).
MOLECULAR BIOLOGY TECHNIQUES.
BACTERIAL TRANSFORMATION.
PLASMID CULTURE AND DNA ISOLATION.
MINIPREPARATION OF PLASMID DNA.
BACTERIAL GROWTH AND PLASMID ISOLATION.
NUCLEIC ACID GEL ELECTROPHORESIS.
PREPARATION OF CDNA PROBE.
PREPARATION OF TOTAL RNA AND NORTHERN ANALYSIS.
STAINING OF APOPTOTIC CELLS.
PHOTOGRAPHS.
STATISTICAL ANALYSIS.
21
21
21
21
22
22
22
22
23
23
24
24
.25
,25
.26
.26
.27
.27
.27
.28
.28
.30
.31
.32
.32
CHAPTER IV:
CALPAIN II AND PKC REGULATION DURING THE PNEUMOTOXICITY
CAUSED BY ACUTE AND CHRONIC BHT TREATMENT.33
.33
.33
.33
INTRODUCTION.
BUTYLATED HYDROXYTOLUENE (BHT).
BHT AND MOUSE LUNG.
V HISTOLOGICAL CHANGES INDUCED BY A SINGLE BHT INJECTION.3^
OTHER MEASUREMENTS FOR ASSESSMENT OF BHT INDUCED LUNG DAMAGE.
3^
BIOMARKERS OF TOXICITY.
D
TUMOR PROMOTION.3^
TABIF. OF CONTENTS
XLLL
RESULTS.
LUNG TUMOR INDUCTION AND PNEUMOTOXICITY.
PKC AND CALPAIN II CONCENTRATIONS.
PKC-ISOZYME ANALYSIS.
TIME COURSE OF THE EFFECTS OF ACUTE AND CHRONIC BHT TREATMENT
ON PKC AND CALPAIN.
REQUIREMENT FOR
BHT METABOLISM.
CALPAIN MRNA LEVELS FOLLOWING BHT TREATMENT.
EXTENT OF BHT-INDUCED LUNG DAMAGE CORRELATES WITH ENZYMES
CHANGES.
DOSE-DEPENDENCE OF BHT-INDUCED DOWN-REGULATION OF CALPAIN
EARLY TIME COURSE AFTER A SINGLE BHT INJECTION.
DISCUSSION.
PHYSIOLOGICAL CONSEQUENCES OF STRAIN-SPECIFIC ENZYMATIC
DOWN-REGULATION.
MECHANISMS GENERATING THESE STRAIN DIFFERENCES.
36
36
37
39
40
42
42
43
44
45
46
.46
.47
CHAPTER V: THE ROLE OF CALPAIN IN THE EFFECTS OF THE TUMOR PROMOTER
1
2-O-TETRADECANOYL
13
-PHORBOL ACETATE ON MOUSE LUNG EPITHELIAL
CELLS.
INTRODUCTION.
12-D-TETRADECANOYL-13-PHORBOL ACETATE (TPA).
TPA AND GENE EXPRESSION.
TPA AND PROTEIN KINASE .
MOUSE LUNG EPITHELIAL CELL LINES.
RESULTS.
CALPAIN II AND PKC ISOZYME CONTENT OF EPITHELIAL CELL LINES.
TPA EFFECTS ON CALPAIN II AND PKC SUBCELLULAR DISTRIBUTION.
TIME COURSES OF CHANGES.
BASAL CALPAIN II LEVELS INFLUENCE CALPAIN II UP-REGULATION.
CALPAIN AND PKC INHIBITOR STUDIES.
MORPHOLOGY CHANGES INDUCED BY TP A IN THE PRESENCE AND
ABSENCE OF INHIBITORS.
TPA-INDUCED CHANGES IN CALPAIN AND PKC CONTENT IN THE PRESENCE AND
ABSENCE OF INHIBITORS.
DISCUSSION.
.51
.51
.51
.52
.
52
.53
.53
.53
.55
.55
.57
.57
.57
.59
.61
TABLE OF CON
TENTS---
CHAPTER
VI:
THE ROLE OF CALPAIN IN APOPTOSIS.
65
INTRODUCTION.^
RESULTS.
CALPAIN ACTIVITY DURING DEXAMETHASONE-INDUCED APOPTOSIS.67
IMMUNOBLOT OF CALPAIN AFTER DEXAMETHASONE TREATMENT.69
EFFECT OF CALPAIN INHIBITORS ON DEXAMETHASONE-TREATED THYMOCYTES
.70
_ . .71
DISCUSSION.
CHAPTER
VII:
THE BENZENE METABOLITE HYDROQUINONE DECREASES
INTERLEUKIN
LOT
CONVERSION
BY
DOWN-REGULATION OF CALPAIN II.73
73
INTRODUCTION.
RESULTS.^
DISCUSSION.
CHAPTER VIII: THE GROWTH STATE OF EPITHELIAL CELLS AFFECTS CALPAIN II
.77
CONTENT.
77
INTRODUCTION.
77
RESULTS.
~ . .78
DISCUSSION.
CHAPTER
IX:
FINAL CONCLUSIONS
.81
BIBLIOGRAPHY
83 |
| any_adam_object | 1 |
| author | Miller, Anita C. |
| author_facet | Miller, Anita C. |
| author_role | aut |
| author_sort | Miller, Anita C. |
| author_variant | a c m ac acm |
| building | Verbundindex |
| bvnumber | BV009526148 |
| ctrlnum | (OCoLC)59821027 (DE-599)BVBBV009526148 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV009526148</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">940329s1994 gw ad|| m||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">940537044</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)59821027</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV009526148</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-91</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Miller, Anita C.</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Studies on the physiological role of the calcium-dependent protease, calpain</subfield><subfield code="c">von Anita C. Miller</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1994</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XVII, 104 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">Karlsruhe, Univ., Diss., 1994</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006289947&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-006289947</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV009526148 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T16:20:36Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-006289947 |
| oclc_num | 59821027 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-11 DE-188 |
| owner_facet | DE-91 DE-BY-TUM DE-11 DE-188 |
| physical | XVII, 104 S. Ill., graph. Darst. |
| publishDate | 1994 |
| publishDateSearch | 1994 |
| publishDateSort | 1994 |
| record_format | marc |
| spelling | Miller, Anita C. Verfasser aut Studies on the physiological role of the calcium-dependent protease, calpain von Anita C. Miller 1994 XVII, 104 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Karlsruhe, Univ., Diss., 1994 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006289947&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Miller, Anita C. Studies on the physiological role of the calcium-dependent protease, calpain |
| subject_GND | (DE-588)4113937-9 |
| title | Studies on the physiological role of the calcium-dependent protease, calpain |
| title_auth | Studies on the physiological role of the calcium-dependent protease, calpain |
| title_exact_search | Studies on the physiological role of the calcium-dependent protease, calpain |
| title_full | Studies on the physiological role of the calcium-dependent protease, calpain von Anita C. Miller |
| title_fullStr | Studies on the physiological role of the calcium-dependent protease, calpain von Anita C. Miller |
| title_full_unstemmed | Studies on the physiological role of the calcium-dependent protease, calpain von Anita C. Miller |
| title_short | Studies on the physiological role of the calcium-dependent protease, calpain |
| title_sort | studies on the physiological role of the calcium dependent protease calpain |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006289947&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT milleranitac studiesonthephysiologicalroleofthecalciumdependentproteasecalpain |