Allozyme electrophoresis: a handbook for animal systematics and population studies
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Sydney u.a.
Acad. Pr.
1986
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 410 S. |
| ISBN: | 0125878400 |
Internformat
MARC
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Datensatz im Suchindex
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ALLOZYME
ELECTROPHORESIS
A Handbook for Animal Systematics and Population Studies
B J RICHARDSON
BureaifoTFlora and Fauna
Canberra
P R BAVERSTOCK and M ADAMS
South Australian Museum
Adelaide
ACADEMIC PRESS
(Harcourt Brace Jovanovich, Publishers)
Sydney Orlando San Diego New York Austin
London Montreal Tokyo Toronto
CONTENTS
General Introduction xi
Part I Project Planning 1
Introduction to Part I 1
Chapter 1 Genetics 3
1 1 Elementary Genetics 3
1 2 Population Genetics 8
121 Non-random mating 10
122 Small population size 11
123 Differential selection 11
1 3Clines 12
Chapter 2 Electrophoresis IS
2 1 Protein Structure and Function 15
2 2 Electrophoresis 16
2 3 Staining Systems 20
2 4 Multiple Forms of Enzymes 22
241 The presence of more than one locus 22
242 The presence of more than one allele 22
243 Post-translational modifications
s 24
2 5 Allelic Variation 26
2 6 Applications of Allozyme Electrophoresis 28
Chapter 3 Population Structures 31
3 1 Alternative Population Models 32
3 2 Differentiating Between Population Models in
Simple Situations 35
3 3 More Complex Field Situations 36
331 Overlapping subpopulations 36
332 Very small subpopulations 37
333 Failure to meet isolation-by-distance model assumptions 38
3 4 Sampling Strategies 39
vi CONTENTS
Chapter 4 Systematics 40
4 1 Species 40
4 2 Supraspecific Categories 41
4 3 Electrophoresis in Systematics 46
431 Delineation of biological species 46
432 Phylogenetic reconstruction 53
Chapter 5 Statistical Methods 57
5 1 Binomially Distributed Data 57
5 2 Hypothesis Testing 58
5 3 Setting Sample Sizes for Population Studies 60
5 4 Testing for Differences in Allele Frequency 62
5 5 Testing the Assumption that a Single Panmictic Population
is Sampled 65
5 6 Testing the Assumption that Alleles at a Single Locus
are being Studied 69
5 7 Tests for the Independence of Loci 69
5 8 Measures of Genetic Relatedness for use in Population Studies 70
5 9 Sample Sizes in Taxonomic Studies 74
Chapter 6 Project Planning and Sampling Strategies 76
6 1 Population Studies 76
6 2 Systematic Studies 79
621 Delineating species boundaries 80
622 Phylogenetic studies 82
Part II Collecting the Data 83
Introduction to Part II 83
Chapter 7 Sample Collection, Handling and Preparation 85
7 1 Background 85
7 2 General Principles of Sampling 87
7 3 Tissues 89
7 4 Taking and Processing 91
7 5 Preserving 93
7 6 Transport 94
7 7 Long-term Storage 94
7 8 Preparation of Samples for Electrophoresis 95
7 9 Compromises in Sampling Technique 98
Chapter 8 Electrophoresis Methods 99
8 1 Choice of Electrophoretic Support Medium 99
8 2 General Laboratory Requirements 102
CONTENTS vii
821 Equipment 102
822 Consumables 103
8 3 Standard Electrophoretic Procedures 104
831 Pre-loading 113
832 Loading 115
833 The electrophoretic run 118
834 Staining 119
835 Scoring 122
8 4 Genetic Interpretation of Zymograms 127
841 Factors responsible for complex electrophoretic patterns 128
842 Practical interpretation procedures 136
843 General principles of interpretation 140
844 Line-up gels 142
845 Integration of results across gels 143
Chapter 9 Specific Enzyme Methods for Cellulose Acetate
Electrophoresis 145
9 1 The Structure of the Enzyme Sheets 145
911 General background 145
912 Standardized nomenclature 146
913 Consumables 148
914 The format of the enzyme sheets 148
9 2 The Sheets 161
9 3 Explanatory Notes 219
931 Note 1: Buffers 219
932 Note 2: Stabilizers 219
933 Note 3: Common detection
mechanisms 220
934 Note 4: Isozyme mobility \ 221
935 Note 5: Formazan precipitation 221
936 Note 6: Inhibition of phantom
dehydrogerases 221
937 Note 7: Arsenate 222
938 Note 8: Post-coupling 222
939 Note 9: NAD-dependent G6PD 222
9 3 10 Note 10: Fluorescent detection
systems 222
9 3 11 Note 11: Cytoplasmic and mitochondrial
enzymes 224
9 3 12 Note 12: Multiple locus systems 225
9 3 13 Note 13: Hybridization between the
isozyme products of separate loci 225
viii CONTENTS
9 3 14 Note 14: Treatment-related modification 226
9 3 15 Note 15: Complex electrophoretic patterns 226
9 3 16 Note 16: Warping 227
9 3 17 Note 17: Uniriterpretable variation 227
9 3 18 Note 18: Atypical heterozygotes 227
9 3 19 Note 19: Stain artifacts 228
Chapter 10 Strategies 229
10 1 Setting Up a New Electrophoresis Unit 229
10 1 1 Background concepts 229
10 1 2 Learning strategies for cellulose acetate 231
10 2 Enzyme Suitability 235
10 2 1 Presence 235
10 2 2 Staining intensity 236
10 2 3 Electrophoretic resolution 236
10 2 4 Reliability 237
10 2 5 Cost of stain 237
10 2 6 Tissue distribution 237
10 2 7 Number of loci 237
10 2 8 Tissue-specific loci 237
10 2 9 Electrophoretic conservativeness 238
10 2 10 Summary 238
10 3 Laboratory Strategies 238
10 3 1 Routine screen 239
10 3 2 Method modification 239
10 3 3 Further investigation 242
10 3 4 Line-ups 243
10 3 5 Random retyping 243
10 3 6 Reinterpretation 243
10 3 7 General laboratory protocol 243
10 3 8 Investigative work on multiple-locus systems 244
10 3 9 Electromorph splitting 249
10 4 Specific Resource-Saving Strategies 251
10 4 1 Tissue handling ' 251
10 4 2 Test strips 251
10 4 3 Choice of running buffers 252
10 4 4 Choice of tissues for screening 253
10 4 5 Pre-loading strategies 254
10 4 6 Selection of loading order 254
10 4 7 Mobility controls 256
10 4 8 Origin placements for cellulose acetate electrophoresis 256
10 4 9 Multiple origins 257
CONTENTS ix
10 4 10 Multiple loading 258
10 4 11 Run strategies 258
10 4 12 Staining strategies — 260
10 4 13 Staining weak activity enzymes 261
10 4 14 Double stains 261
10 4 15 Freezing gels 264
10 4 16 Trouble-shooting 264
10 4 17 Stain trouble-shooting 268
10 4 18 Large-scale genetic typing exercises 269
10 4 19 Inter-laboratory consultation 270
Part III Analysing the Data 271
Introduction to Part III 271
Chapter 11 Population Analysis using Electrophoretic Data 273
11 1 Analysis of the Sample Set Data 274
11 1 1 Locus-specific phenomena 277
11 1 2 Population-specific phenomena 279
11 2 Within-Stock and Between-Stock Analysis 280
11 2 1 Comparisons between replicate sample sets 280
11 2 2 Comparisons within stocks 280
11 2 3 Comparisons of different stocks 282
11 2 4 Geographical analysis of allele frequencies 282
11 3 Further Analysis of Geographical Structuring 283
11 3 1 Theoretical relationships between genetic and
geographical distance 287
11 3 2 The effects of other factors 288
11 3 3 Mapping the distributions of subpopulations 288
11 3 4 Estimating neighbourhood size 289
11 4 Examples of Analysis - 290
11 4 1 The jackass morwong (Cheilodactylus macropterus) 290
11 4 2 The Australian snapper (Chrysophrys auratus) 293
11 4 3 The European rabbit in Australia 295
11 4 4 Brown Trout (Salmo trutta) in Scandinavia 298
11 4 5 Skipjack tuna (Katsuwonuspelamis) in the Bismark Sea 299
Chapter 12 Analysis of Electrophoretic Data in Systematics 303
12 1 Dissection of Species Complexes 303
12 1 1 Sympatric sibling species 303
12 1 2 Allopatric populations 304
12 2 Phylogenetic Reconstruction 309
12 2 1 Phenetic analysis 309
x CONTENTS
12 2 2 Numerical methods based on distance data 312
12 2 3 The Hennigian approach 316
12 2 4 Reticulate evolution 325
12 2 5 The molecular clock 326
12 3 Integration With Other Data 327
12 3 1 Phylogenetic analysis 327
12 3 2 Species complexes 328
12 4 A Worked Example of Phylogenetic Analysis • 330
12 4 1 Distance analysis 331
12 4 2 Hennigian analysis 337
12 4 3 Synthesis 345
12 4 4 Details of computer programs 346
Part IV Other Types of Project 347
Chapter 13 Other Biological Uses for Allozyme Electrophoresis 349
13 1 Genetic Identification 349
13 1 1 Genetic identification of an individual 349
13 1 2 Species identification 349
13 2 Genetic Quality Control of Laboratory Animal Stocks 351
13 2 1 Background 351
13 2 2 Project planning 351
, 13 2 3 Interpretation 352
13 3 Cell Culture Contamination 353
13 4 Parental Testing 353
13 5 Relating a Tissue to an Individual 354
13 6 Identifying Cryptic Species 354
13 7 Identifying Non-adult Forms 355
13 8 Identifying Meat Substitutions 355
13 9 Gene Mapping 356
13 10 Origins and Relationships of Domestic Forms 357
13 11 Assessing the Loss of Variability Due to Inbreeding 357
13 12 Applications in Protozoology and Bacteriology 358
13 13 Applications of Allozyme Electrophoresis to Plants 360
13 13 1 Ploidy level 360
13 13 2 Breeding systems 361
13 13 3 Chemical defence systems 361
Appendix I 363
Appendix II 367
Bibliography 389
Index 401 |
| any_adam_object | 1 |
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| indexdate | 2024-12-06T17:00:27Z |
| institution | BVB |
| isbn | 0125878400 |
| language | English |
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| physical | XII, 410 S. |
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| spelling | Richardson, B. J. Verfasser aut Allozyme electrophoresis a handbook for animal systematics and population studies B. J. Richardson, P. R. Baverstock and M. Adams Sydney u.a. Acad. Pr. 1986 XII, 410 S. txt rdacontent n rdamedia nc rdacarrier Electrophoresis Technique Electrophoresis methods Enzymes Analysis Technique Enzymes analysis Genetics methodology Population genetics Technique Elektrophorese (DE-588)4014373-9 gnd rswk-swf Populationsgenetik (DE-588)4046804-5 gnd rswk-swf Genetische Variabilität (DE-588)4264352-1 gnd rswk-swf Enzym (DE-588)4014988-2 gnd rswk-swf Elektrophorese (DE-588)4014373-9 s Enzym (DE-588)4014988-2 s Genetische Variabilität (DE-588)4264352-1 s DE-604 Populationsgenetik (DE-588)4046804-5 s Baverstock, P. R. Verfasser aut Adams, M. Verfasser aut HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=006132623&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Richardson, B. J. Baverstock, P. R. Adams, M. Allozyme electrophoresis a handbook for animal systematics and population studies Electrophoresis Technique Electrophoresis methods Enzymes Analysis Technique Enzymes analysis Genetics methodology Population genetics Technique Elektrophorese (DE-588)4014373-9 gnd Populationsgenetik (DE-588)4046804-5 gnd Genetische Variabilität (DE-588)4264352-1 gnd Enzym (DE-588)4014988-2 gnd |
| subject_GND | (DE-588)4014373-9 (DE-588)4046804-5 (DE-588)4264352-1 (DE-588)4014988-2 |
| title | Allozyme electrophoresis a handbook for animal systematics and population studies |
| title_auth | Allozyme electrophoresis a handbook for animal systematics and population studies |
| title_exact_search | Allozyme electrophoresis a handbook for animal systematics and population studies |
| title_full | Allozyme electrophoresis a handbook for animal systematics and population studies B. J. Richardson, P. R. Baverstock and M. Adams |
| title_fullStr | Allozyme electrophoresis a handbook for animal systematics and population studies B. J. Richardson, P. R. Baverstock and M. Adams |
| title_full_unstemmed | Allozyme electrophoresis a handbook for animal systematics and population studies B. J. Richardson, P. R. Baverstock and M. Adams |
| title_short | Allozyme electrophoresis |
| title_sort | allozyme electrophoresis a handbook for animal systematics and population studies |
| title_sub | a handbook for animal systematics and population studies |
| topic | Electrophoresis Technique Electrophoresis methods Enzymes Analysis Technique Enzymes analysis Genetics methodology Population genetics Technique Elektrophorese (DE-588)4014373-9 gnd Populationsgenetik (DE-588)4046804-5 gnd Genetische Variabilität (DE-588)4264352-1 gnd Enzym (DE-588)4014988-2 gnd |
| topic_facet | Electrophoresis Technique Electrophoresis methods Enzymes Analysis Technique Enzymes analysis Genetics methodology Population genetics Technique Elektrophorese Populationsgenetik Genetische Variabilität Enzym |
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